BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144564.12 + phase: 0
(357 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC231280 220 3e-61
TC218904 119 2e-27
TC221214 41 7e-04
TC225641 similar to GB|AAA18379.1|475719|ATU08467 RNA-binding pr... 33 0.26
TC225640 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, part... 32 0.44
BM527420 31 0.75
TC233840 UP|MATK_SOYBN (Q9TKS6) Maturase K (Intron maturase), co... 31 0.75
TC231731 similar to UP|Q03731 (Q03731) Core protein precursor, p... 30 1.7
TC208420 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (79%) 30 2.2
TC204822 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, part... 30 2.2
TC221817 similar to GB|AAP37839.1|30725634|BT008480 At5g64750 {A... 29 2.9
CD404540 29 3.7
TC205645 UP|Q8RUD9 (Q8RUD9) Seed coat BURP domain protein 1, com... 28 4.9
CF922319 28 6.4
TC222663 similar to UP|Q8LPF7 (Q8LPF7) At1g78800/F9K20_16, parti... 28 6.4
TC205357 similar to UP|Q8LRL5 (Q8LRL5) Nam-like protein 10, part... 28 6.4
TC228019 similar to UP|Q9C5T8 (Q9C5T8) Cytokinin receptor CRE1b,... 28 8.3
TC217340 similar to UP|Q9VAD4 (Q9VAD4) CG7883-PA (HL01112p) (EIF... 28 8.3
TC217404 28 8.3
>TC231280
Length = 866
Score = 220 bits (561), Expect(2) = 3e-61
Identities = 110/133 (82%), Positives = 123/133 (91%)
Frame = +1
Query: 221 MFKQLLELSVEELIAKGNSSLLARRNAASKLLDKVFRVRLGRGFYGECLGVRADGNSNLS 280
M +LL+LS E+LI KGNS + +R+NAASKLL+K FRVRLGRGFYGECLGVRADGNSNLS
Sbjct: 43 MAVKLLKLSAEDLITKGNSCVSSRQNAASKLLEKAFRVRLGRGFYGECLGVRADGNSNLS 222
Query: 281 DEIGMLLSVKSASIGLRPIGAVIFMQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSS 340
DEIG LLSVKSA+ GLRPIGAVIFMQRNNLKMCLRS+D++TDTSEVAKAYGGGGS SSSS
Sbjct: 223 DEIGKLLSVKSAATGLRPIGAVIFMQRNNLKMCLRSTDSSTDTSEVAKAYGGGGSPSSSS 402
Query: 341 FIIRMDEYNQWLS 353
FIIR+DEYNQW+S
Sbjct: 403 FIIRLDEYNQWIS 441
Score = 33.1 bits (74), Expect(2) = 3e-61
Identities = 14/17 (82%), Positives = 15/17 (87%)
Frame = +2
Query: 195 SLPDIKPFNIGLSEWRS 211
SLP I+ FNIGLSEWRS
Sbjct: 2 SLPGIRAFNIGLSEWRS 52
>TC218904
Length = 824
Score = 119 bits (299), Expect = 2e-27
Identities = 68/180 (37%), Positives = 99/180 (54%), Gaps = 6/180 (3%)
Frame = +1
Query: 178 RVELILKYIEDADLRHWSLPDIKPFNIGLSEWRSRFSCISNPYMFKQLLELSVEELIAKG 237
RV + YIED DL W LP+ K F GL + F NP +F QLL L ++ +I+ G
Sbjct: 10 RVRKLFLYIEDGDLWRWRLPNSKAFRSGLKDLDIEFDARKNPSLFDQLLSLDLDNIISTG 189
Query: 238 NSSLLARRNAASKLLDKVFRVRLGRGFYGECLGVRAD-GNSNLSDEIGMLLSVKSASIGL 296
SL ++ L K + + LG +G CL V AD S L E+G L+ KS + L
Sbjct: 190 TVSLSDKQKLIDDCLRKSYEIALGNAEFGYCLAVNADTALSELRSELGHQLATKSQKMNL 369
Query: 297 RPIGAVIF-----MQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSSFIIRMDEYNQW 351
R IGAV++ LK+ LRS +N DT+ +++ +GGGG ++SSF++ DE+ +W
Sbjct: 370 RGIGAVVYNVPELENDQRLKISLRSVEN-EDTTPISQEFGGGGHRNASSFMLTADEFEKW 546
>TC221214
Length = 626
Score = 41.2 bits (95), Expect = 7e-04
Identities = 32/120 (26%), Positives = 57/120 (46%), Gaps = 4/120 (3%)
Frame = +3
Query: 41 VLYNYPSFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAI 100
+LY+YP GAF++L AHL+ + S L F + S ++ L + +
Sbjct: 213 ILYHYPCPDGAFAALAAHLYFKATSSFS-PLFFPNTVFNPLSAEDLPLNEIGDLYLLNFV 389
Query: 101 CLTFFLQTNSL----IIGFDHRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYFTDK 156
F+Q S +I DH K+ L ++ + EN++ ++ E+S + ++YF DK
Sbjct: 390 GPDGFVQEISTKVPRVIVLDHHKTALERLGNEASLGENVVKVIDMERSGATIAFDYFKDK 569
>TC225641 similar to GB|AAA18379.1|475719|ATU08467 RNA-binding protein 2
{Arabidopsis thaliana;} , partial (59%)
Length = 1223
Score = 32.7 bits (73), Expect = 0.26
Identities = 19/51 (37%), Positives = 28/51 (54%)
Frame = +3
Query: 59 LFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFLQTN 109
LF+ ++ SLS+P + + +L F C N L K RH+ LTF QT+
Sbjct: 141 LFYAKNKTLSLSIPTNPL-NLQFPCSNSSLFPLTTKTTRHSSLLTFVAQTS 290
>TC225640 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, partial (66%)
Length = 1290
Score = 32.0 bits (71), Expect = 0.44
Identities = 19/51 (37%), Positives = 27/51 (52%)
Frame = +3
Query: 59 LFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFLQTN 109
LF+ ++N SLS+P + +L C N L K RH+ LTF QT+
Sbjct: 174 LFYAKNNTLSLSIPTKPL-NLHLPCSNSSLFPLTTKTTRHSSLLTFVAQTS 323
>BM527420
Length = 420
Score = 31.2 bits (69), Expect = 0.75
Identities = 16/57 (28%), Positives = 34/57 (59%)
Frame = +3
Query: 136 MINLNHEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKDKGRVELILKYIEDADLR 192
+++ ++++S AVYE F + ++ +NG VP+L D + + + +L E A++R
Sbjct: 240 IVSNGNKRASEMAVYEQFRSEGQNQIHTNGFVPTLTDERPQ---KSLLPPFESAEMR 401
>TC233840 UP|MATK_SOYBN (Q9TKS6) Maturase K (Intron maturase), complete
Length = 1518
Score = 31.2 bits (69), Expect = 0.75
Identities = 25/60 (41%), Positives = 28/60 (46%)
Frame = +1
Query: 208 EWRSRFSCISNPYMFKQLLELSVEELIAKGNSSLLARRNAASKLLDKVFRVRLGRGFYGE 267
+W S F SNP F L L V E S L RN +SKL K FRV R F+ E
Sbjct: 577 KWISTFFSKSNPRFFLFLYNLYVWEY----ESIFLFLRNKSSKLRLKYFRVFFERIFFYE 744
>TC231731 similar to UP|Q03731 (Q03731) Core protein precursor, partial (10%)
Length = 616
Score = 30.0 bits (66), Expect = 1.7
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = +3
Query: 39 NIVLYNYPSFSGAFSSLFAHLFHTRHNLPSLSL--PFSSVPSLAFS 82
N L+ + AFS +F+ L H +H+LP L L P S+ LA S
Sbjct: 354 NTALHFSGFLANAFSFIFSDLHHEKHSLPPLCLSPPTPSLDLLALS 491
>TC208420 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (79%)
Length = 1523
Score = 29.6 bits (65), Expect = 2.2
Identities = 16/57 (28%), Positives = 34/57 (59%)
Frame = +1
Query: 136 MINLNHEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKDKGRVELILKYIEDADLR 192
+++ ++++S AVYE F + + +NG VP++VD + + + +L E A++R
Sbjct: 244 IVSNGNKRASEMAVYEQFRGEGLNQIHTNGFVPTIVDERPQ---KSLLPPFESAEMR 405
>TC204822 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, partial (67%)
Length = 1232
Score = 29.6 bits (65), Expect = 2.2
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = +3
Query: 59 LFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFLQTN 109
LFHT+ PSL P S +L FSC N + + + +TF QT+
Sbjct: 63 LFHTKTK-PSLFTPPSKPLTLQFSCLNSSVSLSLAAPTHRSPLVTFVAQTS 212
>TC221817 similar to GB|AAP37839.1|30725634|BT008480 At5g64750 {Arabidopsis
thaliana;} , partial (24%)
Length = 1008
Score = 29.3 bits (64), Expect = 2.9
Identities = 35/123 (28%), Positives = 51/123 (41%), Gaps = 6/123 (4%)
Frame = +1
Query: 49 SGAFSSLFAHLFH------TRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICL 102
+G SS+ H H + N+PS + PFSS +A S +LK R
Sbjct: 220 TGDSSSVLHHQPHDDDAVTVKPNMPSTT-PFSSTYVVATS---------SLKRSR----- 354
Query: 103 TFFLQTNSLIIGFDHRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYFTDKHEDIKT 162
Q ++ F H S +P++ EC ++ M N AVYEY T E++K
Sbjct: 355 ----QHDTYTPSFSHPPSSFPAMPTSAECSKSGMGN---------AVYEYRTRGTENVKK 495
Query: 163 SNG 165
G
Sbjct: 496 EEG 504
>CD404540
Length = 601
Score = 28.9 bits (63), Expect = 3.7
Identities = 15/57 (26%), Positives = 28/57 (48%)
Frame = -1
Query: 17 YRSLRSDAALEAIAKASEDKVPNIVLYNYPSFSGAFSSLFAHLFHTRHNLPSLSLPF 73
Y++ D +A+ ++K+PN++LY + S +SL+ L H + PF
Sbjct: 538 YKAFGRDIEFSWLARNMQEKIPNLILYLFFS----INSLYLQLLFVIHVISPSCSPF 380
>TC205645 UP|Q8RUD9 (Q8RUD9) Seed coat BURP domain protein 1, complete
Length = 1369
Score = 28.5 bits (62), Expect = 4.9
Identities = 22/68 (32%), Positives = 30/68 (43%), Gaps = 12/68 (17%)
Frame = -2
Query: 97 RHAICLTFFLQTNSLIIG------FDHRKSVLSQIP------STNECPENIMINLNHEKS 144
+H I +T ++ N+LII F H K P STN PE +NH
Sbjct: 744 KHNIRVTHWMTGNNLIISQLPHPIFHHYKLSRLSFPFFLC*ESTNVLPERGNDEINHGLQ 565
Query: 145 SSRAVYEY 152
SRAV+ +
Sbjct: 564 RSRAVFPF 541
>CF922319
Length = 391
Score = 28.1 bits (61), Expect = 6.4
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +1
Query: 56 FAHLFHTRHNLPSLSLPFSSVPSLAFSC 83
F H+ + NLP+L + SS P L+F C
Sbjct: 169 FDHILIAKCNLPNLPIRQSSTPILSFKC 252
>TC222663 similar to UP|Q8LPF7 (Q8LPF7) At1g78800/F9K20_16, partial (43%)
Length = 801
Score = 28.1 bits (61), Expect = 6.4
Identities = 27/101 (26%), Positives = 40/101 (38%), Gaps = 2/101 (1%)
Frame = -1
Query: 8 SFPPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYPSFS--GAFSSLFAHLFHTRHN 65
+ P C G + + A E V N++L PSFS SS ++ L R +
Sbjct: 324 TMPKCSSFGV*RTHKHSERSSFLSAVEQDVMNLILLETPSFSAKSLSSSKYSTLSFNRLS 145
Query: 66 LPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFL 106
P + + V SL + L I + A C+ FFL
Sbjct: 144 KPPAIVKEALVISLCLRTPSGELSIAKAEI---ANCIFFFL 31
>TC205357 similar to UP|Q8LRL5 (Q8LRL5) Nam-like protein 10, partial (46%)
Length = 777
Score = 28.1 bits (61), Expect = 6.4
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = -1
Query: 215 CISNPYMFKQLLELSVEELIAKGNSSLLARR 245
C SNP++ K L++L + + KGN +AR+
Sbjct: 759 CNSNPFLIKILVKLEI*GELNKGNRKKIARK 667
>TC228019 similar to UP|Q9C5T8 (Q9C5T8) Cytokinin receptor CRE1b, partial
(21%)
Length = 1344
Score = 27.7 bits (60), Expect = 8.3
Identities = 23/85 (27%), Positives = 34/85 (39%)
Frame = -1
Query: 49 SGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFLQT 108
+ AF F H HT H L + + PS GL C L FF +
Sbjct: 624 TAAFQEQFCHFLHTSHQLQNFLMHLQR-PS--------GLPHCHQP-------LRFFSRK 493
Query: 109 NSLIIGFDHRKSVLSQIPSTNECPE 133
+ ++H S LS++ S++ PE
Sbjct: 492 EGFLQKWNH*DSCLSRVASSSPFPE 418
>TC217340 similar to UP|Q9VAD4 (Q9VAD4) CG7883-PA (HL01112p) (EIF2B-alpha
protein), partial (32%)
Length = 1349
Score = 27.7 bits (60), Expect = 8.3
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 105 FLQTNSLIIGFDHRKSVLSQIPSTNECPENIM 136
F +T+SL FD+RK V S +PS + +++
Sbjct: 845 FTETSSLASSFDNRKPVRSGLPSVQKTRNSLL 750
>TC217404
Length = 618
Score = 27.7 bits (60), Expect = 8.3
Identities = 14/32 (43%), Positives = 19/32 (58%)
Frame = +3
Query: 46 PSFSGAFSSLFAHLFHTRHNLPSLSLPFSSVP 77
PSF G SSL +FH+ N+ + FSS+P
Sbjct: 159 PSFQGFLSSLLPPIFHSPQNIST----FSSLP 242
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,600,600
Number of Sequences: 63676
Number of extensions: 244154
Number of successful extensions: 1422
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 1405
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1418
length of query: 357
length of database: 12,639,632
effective HSP length: 98
effective length of query: 259
effective length of database: 6,399,384
effective search space: 1657440456
effective search space used: 1657440456
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144564.12