BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144541.1 - phase: 0
(124 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC225635 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 235 4e-63
TC225638 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 234 1e-62
TC225634 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 232 4e-62
TC225636 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 229 2e-61
TC216242 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 226 3e-60
TC216243 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 166 2e-42
TC212660 weakly similar to UP|Q8ISQ5 (Q8ISQ5) Ribosomal protein ... 138 7e-34
BG315730 134 8e-33
CD395795 homologue to SP|Q9M9W1|R22B_ 60S ribosomal protein L22-... 99 4e-22
TC224013 28 1.4
TC222535 similar to UP|OCRL_HUMAN (Q01968) Inositol polyphosphat... 27 3.2
TC204234 similar to PIR|B86367|B86367 protein F26F24.16 [importe... 27 3.2
TC228900 similar to UP|Q8LCM3 (Q8LCM3) Ribosomal protein-like, p... 25 7.0
TC221098 weakly similar to UP|UMP3_ARATH (Q9LEV3) Protein At5g10... 25 7.0
TC230471 similar to UP|Q9SF87 (Q9SF87) F8A24.10 protein (AT3g098... 25 7.0
TC212231 similar to UP|Q8LCM3 (Q8LCM3) Ribosomal protein-like, p... 25 9.2
>TC225635 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
complete
Length = 857
Score = 235 bits (599), Expect = 4e-63
Identities = 117/124 (94%), Positives = 120/124 (96%)
Frame = +3
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRGSA AKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDS++V
Sbjct: 117 MSRGSATAAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSITVV 296
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 120
REK+KI VTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG
Sbjct: 297 REKTKIIVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 476
Query: 121 EEED 124
EEED
Sbjct: 477 EEED 488
>TC225638 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
complete
Length = 742
Score = 234 bits (596), Expect = 1e-62
Identities = 116/124 (93%), Positives = 120/124 (96%)
Frame = +1
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRG AA AKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSV+VT
Sbjct: 271 MSRGGAAAAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVT 450
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 120
REK+KI +TSD NFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRS+YELRYFNIAENEG
Sbjct: 451 REKTKIILTSDCNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSVYELRYFNIAENEG 630
Query: 121 EEED 124
EEED
Sbjct: 631 EEED 642
>TC225634 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
partial (95%)
Length = 720
Score = 232 bits (591), Expect = 4e-62
Identities = 117/125 (93%), Positives = 121/125 (96%), Gaps = 1/125 (0%)
Frame = +2
Query: 1 MSRGSA-AGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSV 59
MSRG A A AKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDS++V
Sbjct: 83 MSRGGAVAAAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSITV 262
Query: 60 TREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENE 119
TREK+KI VTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRS+YELRYFNIAENE
Sbjct: 263 TREKTKIIVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSVYELRYFNIAENE 442
Query: 120 GEEED 124
GEEED
Sbjct: 443 GEEED 457
>TC225636 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
partial (98%)
Length = 444
Score = 229 bits (585), Expect = 2e-61
Identities = 114/122 (93%), Positives = 118/122 (96%)
Frame = +2
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRG AA AKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSV+VT
Sbjct: 77 MSRGGAAAAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVT 256
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 120
REK+KI +TSD NFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRS+YELRYFNIAENEG
Sbjct: 257 REKTKIILTSDCNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSVYELRYFNIAENEG 436
Query: 121 EE 122
EE
Sbjct: 437 EE 442
>TC216242 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
partial (94%)
Length = 667
Score = 226 bits (575), Expect = 3e-60
Identities = 110/118 (93%), Positives = 117/118 (98%)
Frame = +3
Query: 7 AGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKI 66
+GAKGKKKGA+F IDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDS++VTR+KSKI
Sbjct: 69 SGAKGKKKGASFVIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSITVTRDKSKI 248
Query: 67 TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEED 124
TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNK+RS+YELRYFNIAENEGEEED
Sbjct: 249 TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKERSVYELRYFNIAENEGEEED 422
>TC216243 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
partial (70%)
Length = 447
Score = 166 bits (421), Expect = 2e-42
Identities = 80/87 (91%), Positives = 86/87 (97%)
Frame = -1
Query: 38 KFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLR 97
KFLQE IKVGGKAGALGDS++VTR+KSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLR
Sbjct: 447 KFLQESIKVGGKAGALGDSITVTRDKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLR 268
Query: 98 VIASNKDRSIYELRYFNIAENEGEEED 124
VIASNK+R++YELRYFNIAENEGEEED
Sbjct: 267 VIASNKERNVYELRYFNIAENEGEEED 187
>TC212660 weakly similar to UP|Q8ISQ5 (Q8ISQ5) Ribosomal protein L22, partial
(85%)
Length = 470
Score = 138 bits (347), Expect = 7e-34
Identities = 68/118 (57%), Positives = 88/118 (73%)
Frame = +1
Query: 6 AAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSK 65
A KGKK FT++CA PV+D+I DI S EKFL ERIKVGGK G LGD +++ RE+S
Sbjct: 106 AQAKKGKKTSLKFTVNCAAPVDDEIFDIQSFEKFLHERIKVGGKTGVLGDIIAIERERSN 285
Query: 66 ITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEE 123
I VTS + SKRY+KYLTKK+LKK +RD++RVI+S+K + Y+L+YFNI E E+E
Sbjct: 286 IHVTSKTAMSKRYVKYLTKKFLKKQQLRDYIRVISSSK--NAYDLKYFNIHNEEEEQE 453
>BG315730
Length = 244
Score = 134 bits (338), Expect = 8e-33
Identities = 66/80 (82%), Positives = 74/80 (92%)
Frame = +2
Query: 13 KKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDS 72
KKGATFTIDCAKPV+D IMDIASLEKFLQ+RI VGGKAGAL D+++VTREK++I VTSDS
Sbjct: 5 KKGATFTIDCAKPVDDTIMDIASLEKFLQDRINVGGKAGALADTITVTREKTQIIVTSDS 184
Query: 73 NFSKRYLKYLTKKYLKKHNV 92
NF +RYLK LTKKYLKKHNV
Sbjct: 185NFLQRYLKDLTKKYLKKHNV 244
>CD395795 homologue to SP|Q9M9W1|R22B_ 60S ribosomal protein L22-2.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (37%)
Length = 633
Score = 99.4 bits (246), Expect = 4e-22
Identities = 48/65 (73%), Positives = 53/65 (80%)
Frame = -1
Query: 60 TREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENE 119
TR+ ++++ LKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENE
Sbjct: 381 TRD*FSLSLSPSCRIRHEDLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENE 202
Query: 120 GEEED 124
GEEED
Sbjct: 201 GEEED 187
>TC224013
Length = 421
Score = 27.7 bits (60), Expect = 1.4
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = -2
Query: 62 EKSKITVTSDSNFSKRYLKYLTKKYLKKHNV 92
+K+ +TS+SNF RYL K+ K+ NV
Sbjct: 189 QKTXFLITSESNFQ*RYLYKKKKEQCKEKNV 97
>TC222535 similar to UP|OCRL_HUMAN (Q01968) Inositol polyphosphate
5-phosphatase OCRL-1 (Lowe's oculocerebrorenal syndrome
protein) , partial (3%)
Length = 754
Score = 26.6 bits (57), Expect = 3.2
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = +2
Query: 80 KYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAE 117
K ++K K NVR WL I SN D+ + Y+++ E
Sbjct: 254 KKISKSSWPKFNVRKWLN-IRSNDDKFHSDASYYSLPE 364
>TC204234 similar to PIR|B86367|B86367 protein F26F24.16 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(36%)
Length = 1218
Score = 26.6 bits (57), Expect = 3.2
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = -1
Query: 74 FSKRYLKYLTKKYLKKHNVRDWLRVIASNK 103
F+ ++KY+ +YLK++N+ WL +N+
Sbjct: 306 FNHNFIKYVDSEYLKEYNI--WLNKCNTNQ 223
>TC228900 similar to UP|Q8LCM3 (Q8LCM3) Ribosomal protein-like, partial
(98%)
Length = 1033
Score = 25.4 bits (54), Expect = 7.0
Identities = 19/56 (33%), Positives = 26/56 (45%), Gaps = 6/56 (10%)
Frame = +1
Query: 48 GKAGALGDSVSVTREKSK--ITVTSDSNF----SKRYLKYLTKKYLKKHNVRDWLR 97
G L S+SV+R S + + N + R LK+ KK LKK N +W R
Sbjct: 7 GAVAVLSPSLSVSRRLSAS*LGQATKENILL*RTMRKLKFHEKKLLKKVNFLEWKR 174
>TC221098 weakly similar to UP|UMP3_ARATH (Q9LEV3) Protein At5g10860,
mitochondrial precursor, partial (31%)
Length = 882
Score = 25.4 bits (54), Expect = 7.0
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +3
Query: 59 VTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRD 94
+T E + ITVTSD+N K +K +T+ +++ V D
Sbjct: 378 MTDENNLITVTSDTNILKA-MKIMTENHIRHVPVID 482
>TC230471 similar to UP|Q9SF87 (Q9SF87) F8A24.10 protein
(AT3g09850/F8A24_10), partial (10%)
Length = 660
Score = 25.4 bits (54), Expect = 7.0
Identities = 12/37 (32%), Positives = 19/37 (50%)
Frame = +1
Query: 2 SRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEK 38
++ S GAK G F+ AKP +K ++ + EK
Sbjct: 133 NKSSRVGAKSLGVGVEFSNSPAKPAPNKSSNVGAFEK 243
>TC212231 similar to UP|Q8LCM3 (Q8LCM3) Ribosomal protein-like, partial
(34%)
Length = 307
Score = 25.0 bits (53), Expect = 9.2
Identities = 14/32 (43%), Positives = 18/32 (55%), Gaps = 3/32 (9%)
Frame = +1
Query: 69 TSDSNF---SKRYLKYLTKKYLKKHNVRDWLR 97
T +SN + R LK+ KK LKK N +W R
Sbjct: 25 TKESNLL*RTMRKLKFHEKKLLKKVNFLEWKR 120
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.312 0.131 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,707,410
Number of Sequences: 63676
Number of extensions: 32705
Number of successful extensions: 171
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of query: 124
length of database: 12,639,632
effective HSP length: 85
effective length of query: 39
effective length of database: 7,227,172
effective search space: 281859708
effective search space used: 281859708
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144541.1