BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144517.16 - phase: 0
(445 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215130 homologue to UP|O65744 (O65744) GDP dissociation inhibi... 760 0.0
TC215131 homologue to UP|O65744 (O65744) GDP dissociation inhibi... 247 7e-66
BF009173 similar to GP|3175990|emb GDP dissociation inhibitor {C... 137 1e-32
TC215133 homologue to UP|O65744 (O65744) GDP dissociation inhibi... 100 8e-24
BF067342 weakly similar to PIR|T01782|T017 GDP dissociation inhi... 83 3e-16
BG237608 homologue to PIR|T01782|T017 GDP dissociation inhibitor... 83 3e-16
AW508490 similar to GP|3175990|emb| GDP dissociation inhibitor {... 82 6e-16
BG154274 similar to PIR|T01782|T017 GDP dissociation inhibitor -... 74 2e-13
TC219335 weakly similar to UP|Q9LJ18 (Q9LJ18) ESTs AU056916(S209... 65 6e-11
>TC215130 homologue to UP|O65744 (O65744) GDP dissociation inhibitor,
complete
Length = 1747
Score = 760 bits (1963), Expect = 0.0
Identities = 364/444 (81%), Positives = 407/444 (90%)
Frame = +3
Query: 1 MDEEYDVIVLGTGLKECILSGLLSVDGLKVLHMDQNDYYGGASTSLNLTQLFKRYRGDDK 60
MDEEYDVIVLGTGLKECILSGLLSVDGLKVLHMD+NDYYGG STSLNL QL+KR+RGDDK
Sbjct: 123 MDEEYDVIVLGTGLKECILSGLLSVDGLKVLHMDRNDYYGGESTSLNLIQLWKRFRGDDK 302
Query: 61 PPEELGSSREYNVDMIPKFMMANGALVRVLIHTDVTKYLNFKAVDGSFVYNKGKIYKVPA 120
PP LG+SR+YN+DM PKF+MANG LVRVLIHTDVTKYL+FKAVDGSFV+NKGK++KVPA
Sbjct: 303 PPPHLGASRDYNIDMNPKFIMANGNLVRVLIHTDVTKYLSFKAVDGSFVFNKGKVHKVPA 482
Query: 121 TDVEALKSPLMGLFEKRRARKFFIYVQDYEANDPKSHEGLDLNQVTARQLISKYGLEDDT 180
D+EALKSPLMGLFEKRRARKFFIYVQ+Y +DPK+HEG+DL +VT ++LI+KYGL+D+T
Sbjct: 483 NDMEALKSPLMGLFEKRRARKFFIYVQNYNESDPKTHEGMDLTRVTTKELIAKYGLDDNT 662
Query: 181 VDFIGHALALHLDDSYLDKPAKDFVDRVKTYAESLARFQGGSPYIYPLYGLGELPQAFAR 240
+DFIGHALALH DD YL +PA D V R+K YAESLARFQGGSPYIYPLYGLGELPQAFAR
Sbjct: 663 IDFIGHALALHSDDRYLAEPALDTVKRMKLYAESLARFQGGSPYIYPLYGLGELPQAFAR 842
Query: 241 LSAVYGGTYMLNKPECKVEFDGDGKAIGVTSDGETAKCKKVVCDPSYLPDKVQNVGKVAR 300
LSAVYGGTYMLNKPECKVEFD +GK +GVTS+GETAKCKKVVCDPSYLP KV+ VG+VAR
Sbjct: 843 LSAVYGGTYMLNKPECKVEFDEEGKVVGVTSEGETAKCKKVVCDPSYLPGKVRKVGRVAR 1022
Query: 301 AICIMSHPIPDTNDSHSAQVILPQKQLGRKSDMYLFCCSYAHNVAPKGKYIAFVTTEAET 360
AI IMSHPIP T+DSHS Q+ILPQKQLGRKSDMYLFCCSY HNVAPKGK+IAFV+TEAET
Sbjct: 1023AIAIMSHPIPSTDDSHSVQIILPQKQLGRKSDMYLFCCSYTHNVAPKGKFIAFVSTEAET 1202
Query: 361 DQPQVELKPGIDLLGPVDEIFYDIYDRFEPTNDHATDGCFISTSYDPTTHFETTVKDVVQ 420
DQP +ELKPGIDLLGPVDEIF+DIYDR+EP N+ D CF+STSYD TTHFE+TV DV+
Sbjct: 1203DQPAIELKPGIDLLGPVDEIFFDIYDRYEPVNEPTLDNCFVSTSYDATTHFESTVDDVLN 1382
Query: 421 MYSKITGKVLDLSVDLSAASAAAE 444
MY+ ITGKV+DLSVDLSAASAA E
Sbjct: 1383MYTLITGKVIDLSVDLSAASAAEE 1454
>TC215131 homologue to UP|O65744 (O65744) GDP dissociation inhibitor, partial
(32%)
Length = 798
Score = 247 bits (631), Expect = 7e-66
Identities = 117/144 (81%), Positives = 129/144 (89%)
Frame = +1
Query: 301 AICIMSHPIPDTNDSHSAQVILPQKQLGRKSDMYLFCCSYAHNVAPKGKYIAFVTTEAET 360
AI IMSHPIP T+DSHS Q+ILPQKQLGRKSDMYLFCCSY HNVAPKGK+IAFV+TEAET
Sbjct: 1 AIAIMSHPIPSTDDSHSVQIILPQKQLGRKSDMYLFCCSYTHNVAPKGKFIAFVSTEAET 180
Query: 361 DQPQVELKPGIDLLGPVDEIFYDIYDRFEPTNDHATDGCFISTSYDPTTHFETTVKDVVQ 420
DQP +ELKPGIDLLGPVDEIF+DIYDR+EP N+ D CFISTSYD TTHFE+TV DV+
Sbjct: 181 DQPAIELKPGIDLLGPVDEIFFDIYDRYEPVNEPTLDNCFISTSYDATTHFESTVDDVLN 360
Query: 421 MYSKITGKVLDLSVDLSAASAAAE 444
MY+ ITGKV+DLSVDLSAASAA E
Sbjct: 361 MYTLITGKVIDLSVDLSAASAAEE 432
>BF009173 similar to GP|3175990|emb GDP dissociation inhibitor {Cicer
arietinum}, partial (25%)
Length = 418
Score = 137 bits (344), Expect = 1e-32
Identities = 78/140 (55%), Positives = 95/140 (67%)
Frame = +2
Query: 3 EEYDVIVLGTGLKECILSGLLSVDGLKVLHMDQNDYYGGASTSLNLTQLFKRYRGDDKPP 62
EEY VIVLGT L ECIL+GLL V GL VLHMD+NDY GG STSLNL QL++ +RGDDK P
Sbjct: 5 EEYYVIVLGTALTECILTGLLCVHGLIVLHMDRNDYLGGESTSLNLIQLWRTFRGDDK-P 181
Query: 63 EELGSSREYNVDMIPKFMMANGALVRVLIHTDVTKYLNFKAVDGSFVYNKGKIYKVPATD 122
+ R+ + ++ VRVLIHTDVT YL K V GS V++ K++KVPA D
Sbjct: 182 APIRRCRD-STSLLTLSYPG*WQFVRVLIHTDVTMYLYIKDVHGSSVFDIRKVHKVPAHD 358
Query: 123 VEALKSPLMGLFEKRRARKF 142
+ AL PLM L E+RR K+
Sbjct: 359 M*ALMCPLMALLERRRPLKY 418
>TC215133 homologue to UP|O65744 (O65744) GDP dissociation inhibitor, partial
(14%)
Length = 418
Score = 100 bits (248), Expect(2) = 8e-24
Identities = 48/52 (92%), Positives = 50/52 (95%)
Frame = +2
Query: 1 MDEEYDVIVLGTGLKECILSGLLSVDGLKVLHMDQNDYYGGASTSLNLTQLF 52
MDEEYDVIVLGTGLKECILSGLLSVDGLKVLHMD+NDYYGG STSLNL Q+F
Sbjct: 122 MDEEYDVIVLGTGLKECILSGLLSVDGLKVLHMDRNDYYGGESTSLNLIQVF 277
Score = 28.5 bits (62), Expect(2) = 8e-24
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +3
Query: 48 LTQLFKRYRGDDKPP 62
L QL+KR+ GDDKPP
Sbjct: 366 LLQLWKRFXGDDKPP 410
>BF067342 weakly similar to PIR|T01782|T017 GDP dissociation inhibitor -
common tobacco, partial (13%)
Length = 184
Score = 82.8 bits (203), Expect = 3e-16
Identities = 37/61 (60%), Positives = 48/61 (78%)
Frame = +2
Query: 288 LPDKVQNVGKVARAICIMSHPIPDTNDSHSAQVILPQKQLGRKSDMYLFCCSYAHNVAPK 347
LP +V+ VG+V+ AI IMSHPIP ++D+HS Q+I+P Q+G KS+MYLFCCSY H A K
Sbjct: 2 LPRRVRKVGRVSIAIPIMSHPIPTSDDTHSVQIIIPHIQMGLKSNMYLFCCSYTHIAATK 181
Query: 348 G 348
G
Sbjct: 182 G 184
>BG237608 homologue to PIR|T01782|T017 GDP dissociation inhibitor - common
tobacco, partial (13%)
Length = 432
Score = 82.8 bits (203), Expect = 3e-16
Identities = 47/76 (61%), Positives = 54/76 (70%), Gaps = 3/76 (3%)
Frame = +3
Query: 193 DDSYLDKPAK-DFVDRVKTYAESLAR-FQGGSPYIY-PLYGLGELPQAFARLSAVYGGTY 249
D YL +P+ V + + ++SL FQG Y LYGLGELPQAFARLSAVYGGTY
Sbjct: 78 DAXYLAEPSTWTTVKKNEVISQSLLHAFQGXVTLTYIALYGLGELPQAFARLSAVYGGTY 257
Query: 250 MLNKPECKVEFDGDGK 265
MLNKPECKVEFD +GK
Sbjct: 258 MLNKPECKVEFDEEGK 305
Score = 47.8 bits (112), Expect = 1e-05
Identities = 31/62 (50%), Positives = 39/62 (62%), Gaps = 4/62 (6%)
Frame = +2
Query: 169 QLISKYGLEDDTVDFIGHALALHLDDSYLDKPAKDFVDRVKT---YAESLARF-QGGSPY 224
+LI+KYGL+D+T+DFIGHALALH A+ DR + AESLAR + G Y
Sbjct: 2 ELIAKYGLDDNTIDFIGHALALHSR*CAXLG*AEHVDDREEE*SYIAESLARLSRXGHAY 181
Query: 225 IY 226
IY
Sbjct: 182 IY 187
>AW508490 similar to GP|3175990|emb| GDP dissociation inhibitor {Cicer
arietinum}, partial (12%)
Length = 190
Score = 81.6 bits (200), Expect = 6e-16
Identities = 37/58 (63%), Positives = 49/58 (83%)
Frame = +2
Query: 116 YKVPATDVEALKSPLMGLFEKRRARKFFIYVQDYEANDPKSHEGLDLNQVTARQLISK 173
+KVPA ++ALKSPLMGLFEKRRARK FIYV +Y+ +D +HEG+DL +VT ++L+SK
Sbjct: 17 HKVPANYMKALKSPLMGLFEKRRARKLFIYVHNYDESDLSTHEGMDLTRVTTKELVSK 190
>BG154274 similar to PIR|T01782|T017 GDP dissociation inhibitor - common
tobacco, partial (10%)
Length = 142
Score = 73.6 bits (179), Expect = 2e-13
Identities = 37/47 (78%), Positives = 39/47 (82%)
Frame = +2
Query: 2 DEEYDVIVLGTGLKECILSGLLSVDGLKVLHMDQNDYYGGASTSLNL 48
DEEYDVIVLGTGL EC+LSGLLS DGLKVLHMD D+Y G T LNL
Sbjct: 2 DEEYDVIVLGTGLNECMLSGLLSSDGLKVLHMD*TDFY*GEYT*LNL 142
>TC219335 weakly similar to UP|Q9LJ18 (Q9LJ18) ESTs AU056916(S20978), partial
(25%)
Length = 1389
Score = 65.1 bits (157), Expect = 6e-11
Identities = 57/199 (28%), Positives = 91/199 (45%), Gaps = 26/199 (13%)
Frame = +3
Query: 202 KDFVDRVKTYAESLARFQGG-SPYIYPLYGLGELPQAFARLSAVYGGTYMLNKPECKVEF 260
KD +D + Y+ S+ RF +YP+YG GELPQAF R +AV G Y+L +
Sbjct: 21 KDGIDSLALYSSSVGRFPNAPGALLYPIYGEGELPQAFCRRAAVKGCIYVLRMSVVSLLM 200
Query: 261 D---GDGKAIGVTSDGETAKCKKVVCDPSYL-------------PDKVQNV------GKV 298
D G K + + S G+ +++ DPS++ +K+Q + G V
Sbjct: 201 DKVTGSYKGVRLAS-GQDLYSHQLILDPSFMIPSPLSSSPRDFPSEKLQMLSQRDIKGMV 377
Query: 299 ARAICIMSHPIPDTNDSHSAQVILPQKQLGRKSDMYLFCCSYAHNVA--PKGKYIAFVTT 356
AR ICI I D S V+ P + L + + N+A P G +I +++T
Sbjct: 378 ARGICITRSSIKP--DVSSCSVVYPPRSLYPEQVTSIKALQIGSNLAVCPAGTFILYLST 551
Query: 357 EA-ETDQPQVELKPGIDLL 374
+ D+ + LK ++ L
Sbjct: 552 MCNDADEGKKLLKAAMNAL 608
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.138 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,198,899
Number of Sequences: 63676
Number of extensions: 222792
Number of successful extensions: 848
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 847
length of query: 445
length of database: 12,639,632
effective HSP length: 100
effective length of query: 345
effective length of database: 6,272,032
effective search space: 2163851040
effective search space used: 2163851040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144517.16