BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.4 + phase: 0
(360 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE609209 40 0.002
CF920966 35 0.069
TC226398 similar to UP|Q7X998 (Q7X998) MRNA-binding protein (Fra... 34 0.12
TC226399 similar to UP|Q7X998 (Q7X998) MRNA-binding protein (Fra... 33 0.15
TC224025 weakly similar to UP|Q6HXC4 (Q6HXC4) Conserved domain p... 33 0.15
AW832328 33 0.15
AW598319 33 0.15
CF922367 33 0.20
TC222393 homologue to UP|Q7QHP5 (Q7QHP5) AgCP6926 (Fragment), pa... 33 0.20
TC231009 33 0.20
TC225109 weakly similar to UP|Q9V5V8 (Q9V5V8) CG13214-PA, partia... 32 0.34
TC224495 weakly similar to GB|AAO63306.1|28950765|BT005242 At3g2... 32 0.45
TC204871 similar to UP|Q84LL5 (Q84LL5) Cullin 4, partial (81%) 32 0.58
BG239010 homologue to SP|O48928|C773 Cytochrome P450 77A3 (EC 1.... 32 0.58
BF068556 similar to GP|7573443|emb| mRNA binding protein precurs... 32 0.58
TC203850 homologue to UP|GCSP_PEA (P26969) Glycine dehydrogenase... 31 0.76
TC203365 similar to UP|RK9_PEA (P11894) 50S ribosomal protein L9... 31 1.00
BE804044 homologue to GP|14531028|gb| zinc finger/leucine zipper... 31 1.00
BE660008 30 1.7
BG839187 29 2.9
>BE609209
Length = 380
Score = 39.7 bits (91), Expect = 0.002
Identities = 21/36 (58%), Positives = 28/36 (77%), Gaps = 1/36 (2%)
Frame = +1
Query: 326 RKENEKK-QEIKPQNIVVSDFESELEKAIKAAAASS 360
R+E+EKK Q KP ++ +D ESELEKAIKAA ++S
Sbjct: 1 RQEDEKKVQPNKPLSVATTDVESELEKAIKAATSTS 108
>CF920966
Length = 614
Score = 34.7 bits (78), Expect = 0.069
Identities = 23/67 (34%), Positives = 33/67 (48%), Gaps = 1/67 (1%)
Frame = -2
Query: 274 KEMKIDL-ENVEKENKEMKRVLDEMKMGVSKGKVSKQKHAGESVQSWGSNKNGRKENEKK 332
KE K + E +KE KE+ E K G SK E + +K+GRK+ EKK
Sbjct: 409 KERKFPIKEKAKKEGKEIPNQR-ESKKGRKGNSQSKSGRKREKKKGNSQSKSGRKKKEKK 233
Query: 333 QEIKPQN 339
+ +P+N
Sbjct: 232 ENSQPKN 212
Score = 30.4 bits (67), Expect = 1.3
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
Frame = -1
Query: 308 KQKHAGESVQSWGSNKNGRKEN---EKKQEIKPQNIVVSDFESELEKAIK 354
KQK G+ + + +K GRK N ++KQ+ K + + ++E E +K K
Sbjct: 422 KQKKEGKEISNQRESKKGRKGNSQSKRKQKRKERKFPIKEWEKERKKERK 273
>TC226398 similar to UP|Q7X998 (Q7X998) MRNA-binding protein (Fragment),
partial (87%)
Length = 1379
Score = 33.9 bits (76), Expect = 0.12
Identities = 28/71 (39%), Positives = 36/71 (50%), Gaps = 4/71 (5%)
Frame = +1
Query: 67 SYTPTTT----SSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPSFSLH 122
SY P T SS++ FS S S L F P+ S L FS+ SL S S S + PSF +
Sbjct: 34 SYRPMATIASSSSTVLFSAS-SSNLLPFSHPSTSRLSFSS--SLHSSSFSLSISPSFLSY 204
Query: 123 FKPQFGHFSLH 133
+ HF+ H
Sbjct: 205 YSSSSTHFATH 237
>TC226399 similar to UP|Q7X998 (Q7X998) MRNA-binding protein (Fragment),
partial (32%)
Length = 579
Score = 33.5 bits (75), Expect = 0.15
Identities = 26/62 (41%), Positives = 35/62 (55%)
Frame = +2
Query: 72 TTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPSFSLHFKPQFGHFS 131
++SS++ FS S S L F P+ S L FS+ SLSS SSS + PSF + HF+
Sbjct: 95 SSSSTVLFSAS-SSYLLPFSHPSTSRLSFSS--SLSSSSSSLSISPSFLSYTSSNSTHFA 265
Query: 132 LH 133
H
Sbjct: 266 TH 271
>TC224025 weakly similar to UP|Q6HXC4 (Q6HXC4) Conserved domain protein,
partial (15%)
Length = 614
Score = 33.5 bits (75), Expect = 0.15
Identities = 37/114 (32%), Positives = 58/114 (50%), Gaps = 18/114 (15%)
Frame = +1
Query: 27 PITIFNNPLLSTITATNNYSTSDF-SFSLSTNFPTGPTFK----LSYTPTTT-SSSLPFS 80
P + N+PL +++ N S S S++++ P P+ L ++P+++ +SSLPFS
Sbjct: 82 PSSSVNSPLPFCPSSSVNSSLPFCPSSSVNSSLPFSPSSSFNSSLPFSPSSSFNSSLPFS 261
Query: 81 LSLKSGLGLFGSPN---HSPLVFSANFSL---------SSLSSSTPLLPSFSLH 122
S L SP+ +S FSA+ SL SSL+SS P PS S +
Sbjct: 262 PSSSFNSSLPFSPSSSFNSSFAFSASSSLNSSFPFSASSSLNSSFPFSPSCSFN 423
>AW832328
Length = 421
Score = 33.5 bits (75), Expect = 0.15
Identities = 21/56 (37%), Positives = 29/56 (51%)
Frame = -1
Query: 284 EKENKEMKRVLDEMKMGVSKGKVSKQKHAGESVQSWGSNKNGRKENEKKQEIKPQN 339
+KEN + KR E K KQK + QS K+GRK+ EKK+ +P+N
Sbjct: 370 KKENFQSKRKQKEKKGNSQSKSGRKQKEKKGNSQS----KSGRKQKEKKENSQPKN 215
>AW598319
Length = 440
Score = 33.5 bits (75), Expect = 0.15
Identities = 17/68 (25%), Positives = 30/68 (44%)
Frame = +3
Query: 283 VEKENKEMKRVLDEMKMGVSKGKVSKQKHAGESVQSWGSNKNGRKENEKKQEIKPQNIVV 342
VEK KE+ + LDE K + + K K+ ++ G + G Q + + +
Sbjct: 33 VEKARKEIVKKLDEWKASSASNNLDKSKNTDTEIREKGKEREGNVVKPGAQVVDSETVNK 212
Query: 343 SDFESELE 350
F ++LE
Sbjct: 213 KCFSADLE 236
>CF922367
Length = 463
Score = 33.1 bits (74), Expect = 0.20
Identities = 28/90 (31%), Positives = 43/90 (47%)
Frame = -2
Query: 250 ERVEEMKKDEWNNDDNCEGNLQVLKEMKIDLENVEKENKEMKRVLDEMKMGVSKGKVSKQ 309
+RV E KK E N + G Q KE K + ++ ++ K+ + K G KQ
Sbjct: 363 QRVGESKKKEKGNSQSKNGRKQ--KEEKGNSQSKNGRKQKEKKGNSQSKSG------RKQ 208
Query: 310 KHAGESVQSWGSNKNGRKENEKKQEIKPQN 339
K + QS K+GRK+ EKK+ + +N
Sbjct: 207 KEKKGNSQS----KSGRKQKEKKENSQSKN 130
>TC222393 homologue to UP|Q7QHP5 (Q7QHP5) AgCP6926 (Fragment), partial (7%)
Length = 469
Score = 33.1 bits (74), Expect = 0.20
Identities = 34/122 (27%), Positives = 52/122 (41%), Gaps = 6/122 (4%)
Frame = +2
Query: 6 KFQNNNENQQTPQTQIMTAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPTGPT-- 63
K + E ++ P Q KVP LS + + S SFS S+N P PT
Sbjct: 65 KSEERQEKREDPWPQ---GKVPCKSQRRKSLSFLFCLFSASFPFSSFSPSSNLPPPPTTP 235
Query: 64 FKLSYTPTTTSSSLPFSLSLKSG----LGLFGSPNHSPLVFSANFSLSSLSSSTPLLPSF 119
F S + T ++++P + + G GL G+ H +F LS+ S+ P L +
Sbjct: 236 FPRSKSRNTNTTAVPATTPTEHGSTTPRGLRGTTIHCKEIFKGWNCLSAHKSNAPHLSTX 415
Query: 120 SL 121
L
Sbjct: 416 LL 421
>TC231009
Length = 929
Score = 33.1 bits (74), Expect = 0.20
Identities = 28/93 (30%), Positives = 43/93 (46%), Gaps = 5/93 (5%)
Frame = +1
Query: 14 QQTPQTQIMTAKVPITIFNNPLLSTITATNN--YST---SDFSFSLSTNFPTGPTFKLSY 68
Q T K +++F++ IT T N Y T + FS ST +PT T +
Sbjct: 145 QATQMHPFRNPKPCLSLFHHLFRFRITPTTNSPYPTIPSNHFSTDSSTAYPTTTTTTTT- 321
Query: 69 TPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFS 101
T TTTS S+ S + + L+ HSP +++
Sbjct: 322 TKTTTSVSIDAGSSNRKPISLWPGMFHSPAIYA 420
>TC225109 weakly similar to UP|Q9V5V8 (Q9V5V8) CG13214-PA, partial (8%)
Length = 952
Score = 32.3 bits (72), Expect = 0.34
Identities = 31/112 (27%), Positives = 39/112 (34%), Gaps = 9/112 (8%)
Frame = +2
Query: 90 FGSPNHSPLVFSANFSLSSLSSSTPLL----PSFSLHFKPQFGHF-----SLHKTVLSDS 140
+ SPN+ P + S ++ TPLL P F L K SL + S S
Sbjct: 50 YSSPNYLPFLNSQSYHTHKHMLQTPLLQHKNPKFVLQHKKMKPTITILLLSLLLLITSPS 229
Query: 141 SNTNPLSVSPQIEKGFVPETNGSCSGWQNLNLEPFGHNNNNNVGVGVGVGVG 192
T P S Q++ HN NNN G G G G G
Sbjct: 230 LATRPASNPDQVK-----------------------HNKNNNQGGGAGAGAG 316
>TC224495 weakly similar to GB|AAO63306.1|28950765|BT005242 At3g26470
{Arabidopsis thaliana;} , partial (35%)
Length = 751
Score = 32.0 bits (71), Expect = 0.45
Identities = 30/90 (33%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = -2
Query: 58 FPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSSL-SSSTPLL 116
FP P ++ PTT SSLP ++SL F PN + + S S + L +SST
Sbjct: 600 FPEPPIPEI---PTTQRSSLPITISLTL---FFPKPNPTAELLSTTSSSTLLTASSTHPP 439
Query: 117 PSFSLHFKPQFGHFSLHKTVLSDSSNTNPL 146
P + P++ FS +T LS SN + +
Sbjct: 438 PPTPIFIFPKY--FSNRRTELSTRSNRSSI 355
>TC204871 similar to UP|Q84LL5 (Q84LL5) Cullin 4, partial (81%)
Length = 2087
Score = 31.6 bits (70), Expect = 0.58
Identities = 29/103 (28%), Positives = 45/103 (43%)
Frame = -1
Query: 22 MTAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPTGPTFKLSYTPTTTSSSLPFSL 81
M+ +P ++F N L ++ N DFS S+ FP F S+ +++SLP +
Sbjct: 1025 MSLNIPSSLFVNWLPHSVF---NLEIIDFSASIEALFPNNSLFARSFL*NASNTSLP-*M 858
Query: 82 SLKSGLGLFGSPNHSPLVFSANFSLSSLSSSTPLLPSFSLHFK 124
+L L P++S SS PLLP+ S K
Sbjct: 857 NLNKTRTLSSVPSNS-------------SSEVPLLPARSFSSK 768
>BG239010 homologue to SP|O48928|C773 Cytochrome P450 77A3 (EC 1.14.-.-).
[Soybean] {Glycine max}, partial (27%)
Length = 459
Score = 31.6 bits (70), Expect = 0.58
Identities = 23/47 (48%), Positives = 25/47 (52%)
Frame = +3
Query: 51 SFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLSLKSGLGLFGSPNHSP 97
SF LS +P P PTTTSSSLP L S L LF S N +P
Sbjct: 3 SFPLSLKWPLFP-------PTTTSSSLP---*LSSYLALFSSSNTNP 113
>BF068556 similar to GP|7573443|emb| mRNA binding protein precursor-like
{Arabidopsis thaliana}, partial (19%)
Length = 442
Score = 31.6 bits (70), Expect = 0.58
Identities = 24/66 (36%), Positives = 32/66 (48%), Gaps = 12/66 (18%)
Frame = +2
Query: 90 FGSPNHSPLVFSANFSLSSLSSSTPLLPSFSLHFKPQFGHFSLH------------KTVL 137
F P + L FS+ SLSS SSS + PSFS + HF+ H K VL
Sbjct: 50 FSHPATARLCFSS--SLSSSSSSLSITPSFSSYTASNATHFATHAVFSISASAAEKKKVL 223
Query: 138 SDSSNT 143
D++N+
Sbjct: 224 IDNTNS 241
>TC203850 homologue to UP|GCSP_PEA (P26969) Glycine dehydrogenase
[decarboxylating], mitochondrial precursor (Glycine
decarboxylase) (Glycine cleavage system P-protein) ,
partial (30%)
Length = 1160
Score = 31.2 bits (69), Expect = 0.76
Identities = 28/83 (33%), Positives = 36/83 (42%), Gaps = 8/83 (9%)
Frame = -1
Query: 169 NLNLEPFGHNNNNNVGVGV-GVGVGP------DGKNNEKCGLSPCVGVMARTVMP-VTQG 220
N++ EP G G + G G+ P DGKN KC +P +G M P Q
Sbjct: 347 NISAEPHGAAEMVPSGCDLPGAGMPPVGTTGCDGKNGAKCFFTP-IGPMPGPPPP*GMQN 171
Query: 221 LLLNFRWGVSFPGKSVLKMPYLA 243
+L FRW S P + L P A
Sbjct: 170 VLWRFRWQTSAPIQPGLVSPTCA 102
>TC203365 similar to UP|RK9_PEA (P11894) 50S ribosomal protein L9,
chloroplast precursor (CL13), partial (98%)
Length = 983
Score = 30.8 bits (68), Expect = 1.00
Identities = 17/52 (32%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +2
Query: 271 QVLKEMKIDLENVEKENKEMKRVLDEMKM-GVSKGKVSKQKHAGESVQSWGS 321
Q+LKEMKI+ E +E E K +K ++ + + G ++ G+ Q +GS
Sbjct: 305 QLLKEMKIEEERIEAEKKRVKEEAQQLALIFETVGAFKVKRKGGKGKQIFGS 460
>BE804044 homologue to GP|14531028|gb| zinc finger/leucine zipper protein
DALF isoform C1 {Drosophila melanogaster}, partial (2%)
Length = 401
Score = 30.8 bits (68), Expect = 1.00
Identities = 37/107 (34%), Positives = 46/107 (42%), Gaps = 4/107 (3%)
Frame = +1
Query: 23 TAKVPITIFNNPLLSTITATNNYSTSDFSFSLSTNFPTGPTFKLSYTPTTTSSSLPFSLS 82
TA T F N ++T + STS F S S G + TP SS+ S S
Sbjct: 97 TASATTTPFGN------SSTASASTSAFGASSSAAPLFGGASSAATTPFGGSSTASASTS 258
Query: 83 LKSGLGLFGSPNH----SPLVFSANFSLSSLSSSTPLLPSFSLHFKP 125
G L SP+ SPL FS F+ + SSS+ SFS KP
Sbjct: 259 A-FGASLSASPSSAATTSPL-FSGAFATTGASSSSASTTSFSGFAKP 393
>BE660008
Length = 641
Score = 30.0 bits (66), Expect = 1.7
Identities = 25/70 (35%), Positives = 31/70 (43%), Gaps = 14/70 (20%)
Frame = -3
Query: 63 TFKLSYTPTTTSS--------------SLPFSLSLKSGLGLFGSPNHSPLVFSANFSLSS 108
T K TP+TT S SLP S+ S S + +PL+FS F SS
Sbjct: 600 TTKQMTTPSTTGSLARSNQMLQSKKSPSLPLPFSI-SPFFCEASSSATPLIFSQAFGTSS 424
Query: 109 LSSSTPLLPS 118
+ SS LPS
Sbjct: 423 ILSSAATLPS 394
>BG839187
Length = 609
Score = 29.3 bits (64), Expect = 2.9
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 4/95 (4%)
Frame = +3
Query: 253 EEMKKDEWNNDDNCEGNLQVLKEMKIDLENVEKENKEMKRVLDEMKMGVSKGKV----SK 308
+ M +DE + + + + + +++ DLE +++EN++++ LDE+ + ++
Sbjct: 300 QSMVEDEISPNSEXKETKKEMADLQGDLERIKRENQKLRDTLDEVNTNYNALQMHFMNMM 479
Query: 309 QKHAGESVQSWGSNKNGRKENEKKQEIKPQNIVVS 343
Q+ GE + +G K KKQ ++VS
Sbjct: 480 QERKGEEGEBQQXVSDG-KVKXKKQGQSGGGVLVS 581
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.128 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,619,225
Number of Sequences: 63676
Number of extensions: 286711
Number of successful extensions: 2046
Number of sequences better than 10.0: 115
Number of HSP's better than 10.0 without gapping: 1957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2023
length of query: 360
length of database: 12,639,632
effective HSP length: 98
effective length of query: 262
effective length of database: 6,399,384
effective search space: 1676638608
effective search space used: 1676638608
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144516.4