BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.8 + phase: 0 /pseudo
(879 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 155 1e-37
CD417230 weakly similar to GP|2244915|emb| reverse transcriptase... 82 6e-27
CO982349 112 9e-25
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 68 6e-19
AW757033 67 3e-11
BI972659 62 1e-09
TC212765 60 3e-09
BI471325 58 2e-08
BI315739 37 0.029
BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase ... 36 0.084
TC233219 weakly similar to UP|Q8LKI9 (Q8LKI9) NBS/LRR resistance... 35 0.19
TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {A... 35 0.19
TC212055 34 0.32
BI698930 32 0.93
TC226735 similar to UP|O22165 (O22165) 60S ribosomal protein L30... 30 4.6
BQ297648 similar to GP|25012747|gb| RE67695p {Drosophila melanog... 30 6.0
BQ297454 30 6.0
TC217148 similar to PIR|E96809|E96809 protein F28K19.24 [importe... 30 6.0
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 155 bits (391), Expect = 1e-37
Identities = 95/289 (32%), Positives = 144/289 (48%)
Frame = +1
Query: 67 LDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRFSDQWVKWI 126
L + + A E+I K + K K+D K YD + W++L +M +M FS +W+KWI
Sbjct: 1 LHSALIANEVIDEAK---RSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWI 171
Query: 127 MMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEING 186
CV++ + VLVNG ++ +P RGLRQGDPL+P+LF I EGL+ L+R+
Sbjct: 172 EECVKSASISVLVNGSPTAEFLPQRGLRQGDPLTPFLFNIVAEGLNGLMRRAVEENLYKP 351
Query: 187 VKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCSRN 246
+ N IS L +ADD F + ++ IL ++E +N KS F
Sbjct: 352 YLVGANGVPISILQYADDTIFFGEAAKENVEAIKVILRSFELVSNLRINFAKS-CFGVFG 528
Query: 247 VLTTVHNNIASILGVQVVIGTGKYLGLPSMIGRSKKSTFSFIKDRIWKKINLWSSKSLSK 306
V A+ L ++ YLG+P + + I + +K++ W + +S
Sbjct: 529 VTDQWKQEAANYLHCSLLAFPFIYLGIPIGANSRRCHMWDSIIRKCERKLSKWK*RHISF 708
Query: 307 AGREVLIKFVLQSIPTYFMSLFTHPVSLCDEIERIMNSFWWGHSGGQSR 355
GR LIK VL SIP YF S F P S+ D++ +I +F WG Q +
Sbjct: 709 GGRVTLIKSVLTSIPIYFFSFFRVPQSVVDKLVKI*RTFLWGGGPDQKK 855
>CD417230 weakly similar to GP|2244915|emb| reverse transcriptase like
protein {Arabidopsis thaliana}, partial (7%)
Length = 688
Score = 82.4 bits (202), Expect(2) = 6e-27
Identities = 40/120 (33%), Positives = 65/120 (53%)
Frame = -3
Query: 123 VKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARG 182
V+ + C+ T +L NG+V+ P G+RQ DP++PYLF++C+E LS L+ +
Sbjct: 686 VQLVWYCMSTTTMSLLWNGEVLDHFSPNIGVRQRDPIAPYLFVLCLERLSHLINLVMNKK 507
Query: 183 EINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIF 242
+++ +ISHL F DD LF N+DQ + L + + G VN+ K++ F
Sbjct: 506 L*RSIQVNRGGLLISHLAFVDDLILFVEANMDQVDVINLTLELFSDSSGAKVNVDKTKSF 327
Score = 57.8 bits (138), Expect(2) = 6e-27
Identities = 33/89 (37%), Positives = 48/89 (53%)
Frame = -2
Query: 237 QKSEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLGLPSMIGRSKKSTFSFIKDRIWKKI 296
Q + S+NV V +I+S LG+Q GKYLG+P TF FI D++ K+
Sbjct: 342 QNQKFSFSKNVNWWVEEDISSGLGLQSTDDLGKYLGVPIFHKNPTSHTFDFIIDKVNKRF 163
Query: 297 NLWSSKSLSKAGREVLIKFVLQSIPTYFM 325
+ W S LS GR L K V+ ++P++ M
Sbjct: 162 SRWKSNLLSMVGRLTLTK*VV*ALPSHIM 76
>CO982349
Length = 795
Score = 112 bits (279), Expect = 9e-25
Identities = 70/233 (30%), Positives = 105/233 (45%), Gaps = 2/233 (0%)
Frame = +3
Query: 125 WIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEI 184
WI C+ + + +LVN I++ P RGLRQGDPL+P LF I EGL+ L+R+ R
Sbjct: 69 WIRGCLHSASVSILVNESPINEFSPQRGLRQGDPLAPLLFNIVAEGLTGLMREAVDRKRF 248
Query: 185 NGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCS 244
N + N +S L +ADD F ++ ++ IL +E A G +N +S
Sbjct: 249 NSFLVGKNKEPVSILQYADDTIFFEEATMENMKVIKTILRCFELASGLKINFAESRFGA- 425
Query: 245 RNVLTTVH--NNIASILGVQVVIGTGKYLGLPSMIGRSKKSTFSFIKDRIWKKINLWSSK 302
+ H A L ++ YLG+P + I + K+ W +
Sbjct: 426 --IWKPDHWCKEAAEFLNCSMLSMPFSYLGIPIEANPRCREI*DPIIRKCETKLARWKQR 599
Query: 303 SLSKAGREVLIKFVLQSIPTYFMSLFTHPVSLCDEIERIMNSFWWGHSGGQSR 355
+S GR LI +L ++P YF S F P + ++ I F WG Q R
Sbjct: 600 HISLGGRVTLINAILTALPIYFFSFFRAPSKVVQKLVNIQRKFLWGGGSXQRR 758
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 67.8 bits (164), Expect(2) = 6e-19
Identities = 37/100 (37%), Positives = 57/100 (57%)
Frame = -3
Query: 65 SILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRFSDQWVK 124
+I D T E+I+ + KV G G+ ALK+DI K +D +D +L V+ K +S +
Sbjct: 837 TIKDCTCVTSEVINMLDKKVFG--GNLALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCN 664
Query: 125 WIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLF 164
WI + + + + VNG+ + RG+RQGDPLSP L+
Sbjct: 663 WIRVILLSAKLSISVNGEPVGFFSCQRGVRQGDPLSPSLY 544
Score = 45.4 bits (106), Expect(2) = 6e-19
Identities = 33/146 (22%), Positives = 59/146 (39%), Gaps = 10/146 (6%)
Frame = -2
Query: 197 SHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCS----------RN 246
SH++ ADD +F + + Y+ ++GQ ++ KS+IF +
Sbjct: 451 SHVMHADDIMIFCKGTSRNVHNLINFFQLYKDSFGQVISKHKSKIFIGSIPGQRLRVISD 272
Query: 247 VLTTVHNNIASILGVQVVIGTGKYLGLPSMIGRSKKSTFSFIKDRIWKKINLWSSKSLSK 306
+L H++I Y G+P G+ + + D+I K+ W LS
Sbjct: 271 LLQYFHSDIPF-----------NYFGVPIFKGKPTRRHLYCVADKIKCKLASWKGSILSI 125
Query: 307 AGREVLIKFVLQSIPTYFMSLFTHPV 332
GR L+ V+ + Y S++ V
Sbjct: 124 MGRIQLVNSVIHGMLLYSFSVYAWQV 47
>AW757033
Length = 441
Score = 67.0 bits (162), Expect = 3e-11
Identities = 43/137 (31%), Positives = 65/137 (47%)
Frame = -2
Query: 149 PGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLF 208
P RGLRQGDPL+P LF I EGL+ L+R+ AR + +S L +ADD F
Sbjct: 425 PHRGLRQGDPLAPLLFNIAAEGLTGLMREAVARNHYKSFAVGKYKKPVSILQYADDTIFF 246
Query: 209 FRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCSRNVLTTVHNNIASILGVQVVIGTG 268
++ ++ IL +E A G +N KS F + +V A + ++
Sbjct: 245 GEATMENVRVIKTILRGFELASGLKINFAKSR-FGAISVPD*WRKEAAEFMNCSLLSLPF 69
Query: 269 KYLGLPSMIGRSKKSTF 285
YLG+P ++ T+
Sbjct: 68 SYLGIPIGANPRRRETW 18
>BI972659
Length = 453
Score = 62.0 bits (149), Expect = 1e-09
Identities = 37/142 (26%), Positives = 68/142 (47%)
Frame = +1
Query: 214 DQASKMRAILSTYERAYGQAVNLQKSEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLGL 273
D ++++L +E G +N KS+ H+ A +L + + YLG+
Sbjct: 19 DNIIVLKSMLRGFEMVSGLRINYAKSQFGIVGFQPNWAHD-AAQLLNCRQLDIPFHYLGM 195
Query: 274 PSMIGRSKKSTFSFIKDRIWKKINLWSSKSLSKAGREVLIKFVLQSIPTYFMSLFTHPVS 333
P + S + + + ++ K++ W+ K LS GR LIK VL ++P Y +S F P
Sbjct: 196 PIAVKASSRMVWEPLINKFKAKLSKWNQKYLSMGGRVTLIKSVLNALPIYLLSFFKIPQR 375
Query: 334 LCDEIERIMNSFWWGHSGGQSR 355
+ D++ + +F WG + +R
Sbjct: 376 IVDKLVSLQRTFMWGGNQHHNR 441
>TC212765
Length = 637
Score = 60.5 bits (145), Expect = 3e-09
Identities = 35/129 (27%), Positives = 65/129 (50%)
Frame = +3
Query: 161 PYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMR 220
PYLF++C+E L+ + ++ + + ++ ++ +L+ DD FLF D A
Sbjct: 15 PYLFVLCMERLALCIHDLTSQ*IWRPILVSDDNRLVPYLMLVDDIFLFINAIGD*AYLAS 194
Query: 221 AILSTYERAYGQAVNLQKSEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLGLPSMIGRS 280
L + + +G +NL KS ++CS + + + I++ L + GKYL L + GRS
Sbjct: 195 LTL*NFFQTFGLKLNLDKSSMYCS*RMAPVLRDLISNTLNIVHAPSLGKYLCLKFIHGRS 374
Query: 281 KKSTFSFIK 289
K F ++
Sbjct: 375 KIVDFLLVE 401
>BI471325
Length = 421
Score = 57.8 bits (138), Expect = 2e-08
Identities = 34/83 (40%), Positives = 49/83 (58%)
Frame = +2
Query: 183 EINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIF 242
+I+ VK+ I+SHLL D FLF RT +A ++ IL+T+E + G VNL+KSEI
Sbjct: 5 DIHRVKVHRGITILSHLLSVDFYFLFCRTIDKEADVLKNILTTFEASSGLVVNLRKSEIC 184
Query: 243 CSRNVLTTVHNNIASILGVQVVI 265
SRN ++I LG+ + I
Sbjct: 185 FSRNTSQIARDSIFFHLGMSIWI 253
>BI315739
Length = 442
Score = 37.4 bits (85), Expect = 0.029
Identities = 19/52 (36%), Positives = 25/52 (47%)
Frame = +2
Query: 196 ISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCSRNV 247
ISHL F D+C LF + Q +R +L + G LQKS +NV
Sbjct: 164 ISHLFFPDNCLLFVKATSSQVKLVRDVLQQFCLT*GLKAGLQKSRFLA*KNV 319
Score = 35.4 bits (80), Expect = 0.11
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +3
Query: 158 PLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFF 209
PLSPYLF IC+E L+ L++ +KI N P IS +++ ++F
Sbjct: 48 PLSPYLF*ICMEKLAILIQSKVNEETWEPIKISRNGPGISP-IYSSQIIVYF 200
>BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase (EC
2.7.7.49) (clone AmLi2) - garden snapdragon (fragment),
partial (22%)
Length = 111
Score = 35.8 bits (81), Expect = 0.084
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +1
Query: 129 CVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPY 162
C+ + + +LVNG + P RGLRQG P P+
Sbjct: 7 CLSSASISILVNGSPTEEFKPXRGLRQGGPFGPF 108
>TC233219 weakly similar to UP|Q8LKI9 (Q8LKI9) NBS/LRR resistance
protein-like protein (Fragment), partial (74%)
Length = 781
Score = 34.7 bits (78), Expect = 0.19
Identities = 38/127 (29%), Positives = 57/127 (43%), Gaps = 6/127 (4%)
Frame = +3
Query: 152 GLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFFRT 211
GL QG LSPYLF + ++ L+ +++ +R C +LFADD L
Sbjct: 132 GLHQGSTLSPYLFTLILDVLTEQIQEIVSR--------C--------MLFADDIVLL--- 254
Query: 212 NVDQASKMRAILSTYERA---YGQAVNLQKSEIF-CSRNVLTTVHNNIASILGVQVV--I 265
+ K+ L T+ RA +G ++ KSE C N V N+ I G ++ +
Sbjct: 255 -GESREKLNERLETWRRALETHGFRLSRSKSEYMECKFNKRRRVSNSEVKI-GDHIIPQV 428
Query: 266 GTGKYLG 272
KYLG
Sbjct: 429 TRFKYLG 449
>TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {Arabidopsis
thaliana;} , partial (18%)
Length = 759
Score = 34.7 bits (78), Expect = 0.19
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = +2
Query: 308 GREVLIKFVLQSIPTYFMSLFTHPVSLCDEIERIMNSFWWGHSGGQSR 355
GR LIK VL ++P +S F P + D++ + F WG + +R
Sbjct: 8 GRVTLIKSVLSALPIXXLSFFKIPQRIVDKLVTLQRQFLWGGTQHHNR 151
>TC212055
Length = 776
Score = 33.9 bits (76), Expect = 0.32
Identities = 19/77 (24%), Positives = 37/77 (47%)
Frame = +1
Query: 163 LFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAI 222
LF I EGL+ L+R+ + + + + N ++ L +ADD + ++++
Sbjct: 1 LFNIVAEGLTGLMREALDKSLYSSLMVGKNKIPVNILQYADDTIFLGEATMQNVMTIKSM 180
Query: 223 LSTYERAYGQAVNLQKS 239
L +E A G ++ KS
Sbjct: 181 LRVFELASGLKIHFAKS 231
>BI698930
Length = 384
Score = 32.3 bits (72), Expect = 0.93
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +3
Query: 294 KKINLWSSKSLSKAGREVLIKFVLQSIPTYFMSLFTHPVSL 334
K + LW + LS AGR LI +L S+P + S F P+++
Sbjct: 75 K*LALWKHEVLSFAGRTCLINSMLSSLPLFSFSFFKVPLNV 197
>TC226735 similar to UP|O22165 (O22165) 60S ribosomal protein L30
(At2g44860/T13E15.13), partial (74%)
Length = 936
Score = 30.0 bits (66), Expect = 4.6
Identities = 17/38 (44%), Positives = 25/38 (65%)
Frame = -3
Query: 626 CFLSFLLFLFCYNKIWITRILSSKHYMIYLRTMHLYFV 663
CFL F+LFL+C++ + TRILS + + LRT F+
Sbjct: 454 CFLPFILFLWCFSSL-ATRILSI--FGMALRTFSARFL 350
>BQ297648 similar to GP|25012747|gb| RE67695p {Drosophila melanogaster},
partial (10%)
Length = 422
Score = 29.6 bits (65), Expect = 6.0
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +2
Query: 289 KDRIWKKINLWSSKSLSKAGREVLIKFVLQSIP 321
KDR W + SKSLS A VL+KF ++ P
Sbjct: 293 KDRFWNPLPQTESKSLSSATLLVLVKFPSKASP 391
>BQ297454
Length = 135
Score = 29.6 bits (65), Expect = 6.0
Identities = 13/31 (41%), Positives = 16/31 (50%)
Frame = -1
Query: 317 LQSIPTYFMSLFTHPVSLCDEIERIMNSFWW 347
L SI YF S F P + D++ RI F W
Sbjct: 108 LTSISIYFFSFFRVPKKVVDKLARIQRKFLW 16
>TC217148 similar to PIR|E96809|E96809 protein F28K19.24 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(36%)
Length = 1201
Score = 29.6 bits (65), Expect = 6.0
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Frame = -2
Query: 622 MFGACFLSFLLFLFCY--NKIWITRILSSKHYMIYLRTMHLYF--VVCCGVYGNKETTKC 677
MFG F++ + N +WI + S H +I LR H Y+ V C G +G T C
Sbjct: 567 MFGVVRHEFIIIWCSHGNNNMWIDEFVVSGH-LIMLRIRHDYYFLVGCFGFHGQTTTHAC 391
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.354 0.159 0.546
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,495,553
Number of Sequences: 63676
Number of extensions: 592816
Number of successful extensions: 7391
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 4101
Number of HSP's successfully gapped in prelim test: 387
Number of HSP's that attempted gapping in prelim test: 3175
Number of HSP's gapped (non-prelim): 4700
length of query: 879
length of database: 12,639,632
effective HSP length: 106
effective length of query: 773
effective length of database: 5,889,976
effective search space: 4552951448
effective search space used: 4552951448
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.5 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144477.8