BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.14 + phase: 1 /pseudo
(190 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209288 similar to UP|Q9FZP1 (Q9FZP1) Similarity to heparanase,... 278 1e-75
TC224795 weakly similar to UP|Q9FF10 (Q9FF10) Similarity to hepa... 211 1e-55
TC224773 weakly similar to UP|Q9FF10 (Q9FF10) Similarity to hepa... 203 3e-53
TC217497 46 9e-06
BM732841 similar to GP|10177640|dbj contains similarity to hepar... 34 0.036
AW350854 similar to GP|20197988|gb expressed protein {Arabidopsi... 34 0.047
AI759964 30 0.88
TC208030 similar to UP|SIM1_YEAST (P40472) SIM1 protein precurso... 28 2.0
TC208029 UP|Q9A223 (Q9A223) DNA-binding response regulator, part... 28 2.0
TC231383 28 2.6
BI424128 similar to GP|10178015|db Na+/H+ antiporter-like protei... 27 4.4
TC230296 similar to PIR|F86392|F86392 T1K7.7 protein - Arabidops... 27 5.7
AW350693 27 7.4
AI759932 similar to GP|10177640|dbj contains similarity to hepar... 26 9.7
BU550562 26 9.7
TC206671 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotrans... 26 9.7
>TC209288 similar to UP|Q9FZP1 (Q9FZP1) Similarity to heparanase, partial
(53%)
Length = 1048
Score = 278 bits (710), Expect = 1e-75
Identities = 125/171 (73%), Positives = 147/171 (85%)
Frame = +2
Query: 15 IHGWELGNELCGKGIGISISPYQYANDATILRNIVQEVYREVVQKPLIIAPGGFFDANWF 74
IHGWELGNELCG GIG S++ QYA+D LRNIV+ YR + KPL+IAPGGFFD++WF
Sbjct: 2 IHGWELGNELCGSGIGASVAADQYASDVAALRNIVENAYRGIEPKPLVIAPGGFFDSDWF 181
Query: 75 KKFLNRSEKLADVVTHHIYNLGPGVDDHITEKILDPTYLDGVAGTFSSLKNVLQRSSTTA 134
K+F+++S K ADV+THHIYNLGPGVDDH+TEKILDP+YLDG A TFSSLK +LQ S+ +
Sbjct: 182 KEFISKSGKSADVITHHIYNLGPGVDDHLTEKILDPSYLDGEANTFSSLKGILQSSAASV 361
Query: 135 KAWVGEAGGAWNSGHHLVSDAFVNSFWYFDQLGMSATYSTKTYCRQTLIGG 185
K+WVGEAGGA+NSGHHLVSDAFV SFWY DQLGMSA Y T+TYCRQ+LIGG
Sbjct: 362 KSWVGEAGGAYNSGHHLVSDAFVYSFWYLDQLGMSAVYDTRTYCRQSLIGG 514
>TC224795 weakly similar to UP|Q9FF10 (Q9FF10) Similarity to heparanase,
partial (50%)
Length = 877
Score = 211 bits (538), Expect = 1e-55
Identities = 97/186 (52%), Positives = 125/186 (67%), Gaps = 1/186 (0%)
Frame = +3
Query: 1 AKSFIRYTVSKNYTIHGWELGNELCGKGIGISISPYQYANDATILRNIVQEVYREVVQKP 60
AK FI YT+SK Y I WE GNEL GKGIG S+ QY D L+ I+ +Y+ KP
Sbjct: 120 AKDFINYTISKGYKIDSWEFGNELSGKGIGASVGAAQYGKDLIKLKEILHTLYKNSTFKP 299
Query: 61 LIIAPGGFFDANWFKKFLN-RSEKLADVVTHHIYNLGPGVDDHITEKILDPTYLDGVAGT 119
++APGGF++ WF K L + +V+THH+YNLGPG D+H+ KILDP L +
Sbjct: 300 SLVAPGGFYNKEWFDKLLQVTGPGIVNVLTHHVYNLGPGSDEHLDRKILDPENLSKIESI 479
Query: 120 FSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDAFVNSFWYFDQLGMSATYSTKTYCR 179
FS+L +Q+ + AWVGEAGGA+NSG +S+ FVNSFWY DQLG+++ Y+TK YCR
Sbjct: 480 FSNLSETIQKYGPWSSAWVGEAGGAFNSGGRSISNTFVNSFWYLDQLGIASRYNTKVYCR 659
Query: 180 QTLIGG 185
QTLIGG
Sbjct: 660 QTLIGG 677
>TC224773 weakly similar to UP|Q9FF10 (Q9FF10) Similarity to heparanase,
partial (56%)
Length = 1433
Score = 203 bits (517), Expect = 3e-53
Identities = 96/186 (51%), Positives = 126/186 (67%), Gaps = 1/186 (0%)
Frame = +2
Query: 1 AKSFIRYTVSKNYTIHGWELGNELCGKGIGISISPYQYANDATILRNIVQEVYREVVQKP 60
A +FI YT+SK Y I WELGNEL GKGIG S+ QY D L+ I+ +Y KP
Sbjct: 71 AYNFIEYTISKGYKIDSWELGNELSGKGIGASVGVAQYGKDLIKLKQILSTLYENSKFKP 250
Query: 61 LIIAPGGFFDANWFKKFLNRSEK-LADVVTHHIYNLGPGVDDHITEKILDPTYLDGVAGT 119
++APGGF++ +W+ + L S + +V+THH+YNLGPG D+H+ KILDP L V
Sbjct: 251 SLVAPGGFYEKHWYDRLLQVSGSGIINVLTHHLYNLGPGSDEHLERKILDPERLSRVESI 430
Query: 120 FSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDAFVNSFWYFDQLGMSATYSTKTYCR 179
F +L ++ + AWVGEAGGA+NSG + VS+ F+NSFWY DQLG+++ YSTK YCR
Sbjct: 431 FGNLSETIKIYGPWSSAWVGEAGGAYNSGGNHVSNRFLNSFWYLDQLGIASCYSTKVYCR 610
Query: 180 QTLIGG 185
QTLIGG
Sbjct: 611 QTLIGG 628
>TC217497
Length = 1108
Score = 46.2 bits (108), Expect = 9e-06
Identities = 20/43 (46%), Positives = 29/43 (66%)
Frame = +1
Query: 120 FSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDAFVNSFWY 162
F +L ++ + AWVGEAG A+NSG + VS+ F+NSFW+
Sbjct: 79 FGNLSKTIKIYGPCSSAWVGEAGEAYNSGGNHVSNRFLNSFWW 207
Score = 42.4 bits (98), Expect = 1e-04
Identities = 17/24 (70%), Positives = 20/24 (82%)
Frame = +1
Query: 162 YFDQLGMSATYSTKTYCRQTLIGG 185
Y DQ G+++ YSTK YCRQTLIGG
Sbjct: 382 YLDQFGIASCYSTKVYCRQTLIGG 453
>BM732841 similar to GP|10177640|dbj contains similarity to
heparanase~gene_id:MGG23.2 {Arabidopsis thaliana},
partial (16%)
Length = 421
Score = 34.3 bits (77), Expect = 0.036
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = +1
Query: 170 ATYSTKTYCRQTLIGG 185
A Y TKTYCRQTLIGG
Sbjct: 1 AAYDTKTYCRQTLIGG 48
>AW350854 similar to GP|20197988|gb expressed protein {Arabidopsis thaliana},
partial (35%)
Length = 538
Score = 33.9 bits (76), Expect = 0.047
Identities = 20/47 (42%), Positives = 25/47 (52%), Gaps = 7/47 (14%)
Frame = -1
Query: 145 WNSGHHLVSDA-----FVNSFWYFDQLGMSATYSTKTYC--RQTLIG 184
W S HH V + FVN F F+ +G S +Y+ TYC TLIG
Sbjct: 328 WKSYHHFVGYSCVVLGFVNVFQGFEVMGASRSYAKLTYCLGLSTLIG 188
>AI759964
Length = 109
Score = 29.6 bits (65), Expect = 0.88
Identities = 14/20 (70%), Positives = 14/20 (70%)
Frame = +2
Query: 169 SATYSTKTYCRQTLIGGTTV 188
SA Y TKTY RQ LIGG V
Sbjct: 2 SAAYDTKTYRRQMLIGGNYV 61
>TC208030 similar to UP|SIM1_YEAST (P40472) SIM1 protein precursor, partial
(4%)
Length = 800
Score = 28.5 bits (62), Expect = 2.0
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = +2
Query: 110 PTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWN 146
P +L +A + SSL N+L+ SS + G G WN
Sbjct: 335 PVFLSNMASSSSSLSNILESSSVS-----GSEFGCWN 430
>TC208029 UP|Q9A223 (Q9A223) DNA-binding response regulator, partial (6%)
Length = 614
Score = 28.5 bits (62), Expect = 2.0
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = +3
Query: 110 PTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWN 146
P +L +A + SSL N+L+ SS + G G WN
Sbjct: 126 PVFLSNMASSSSSLSNILESSSVS-----GSEFGCWN 221
>TC231383
Length = 730
Score = 28.1 bits (61), Expect = 2.6
Identities = 19/62 (30%), Positives = 28/62 (44%), Gaps = 6/62 (9%)
Frame = +1
Query: 79 NRSEKLADVVTHHIYNLG------PGVDDHITEKILDPTYLDGVAGTFSSLKNVLQRSST 132
N+S K ++V HH N+G P +HI +KI + V T K R++T
Sbjct: 1 NKSTKHNEMVQHHNINMGFMGQSKPPTTEHIVDKINESEASSDVNTTLGLSKYNAYRAAT 180
Query: 133 TA 134
A
Sbjct: 181 MA 186
>BI424128 similar to GP|10178015|db Na+/H+ antiporter-like protein
{Arabidopsis thaliana}, partial (16%)
Length = 421
Score = 27.3 bits (59), Expect = 4.4
Identities = 16/46 (34%), Positives = 21/46 (44%), Gaps = 2/46 (4%)
Frame = +1
Query: 123 LKNVLQRSSTTAKAWVGEAGGA--WNSGHHLVSDAFVNSFWYFDQL 166
LK +RSST G WN GHH S+ + +F + QL
Sbjct: 142 LKEFSERSSTILMVHKARRNGLPFWNKGHHADSNHVIVAFEAYRQL 279
>TC230296 similar to PIR|F86392|F86392 T1K7.7 protein - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (71%)
Length = 1103
Score = 26.9 bits (58), Expect = 5.7
Identities = 20/105 (19%), Positives = 41/105 (39%), Gaps = 10/105 (9%)
Frame = +2
Query: 2 KSFIRYTVSKNYTIHGWELGNELCGK----------GIGISISPYQYANDATILRNIVQE 51
K +I + + G+++G + G+ + PY A++ + V +
Sbjct: 476 KHWITFNEPHTFATQGYDVGLQAPGRCSILLHLFCRAGNSATEPYIVAHNVLLSHATVAD 655
Query: 52 VYREVVQKPLIIAPGGFFDANWFKKFLNRSEKLADVVTHHIYNLG 96
+YR+ +K + G FD W++ N E + + LG
Sbjct: 656 IYRKKYKKIQGGSLGVAFDVIWYEPLTNTKEDIDAAQRAQDFQLG 790
>AW350693
Length = 563
Score = 26.6 bits (57), Expect = 7.4
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = -1
Query: 148 GHHLVSDAFVNSFWYFDQLGMSATYSTKT 176
G LV D+F++S WY Q ++ Y T
Sbjct: 491 GIFLVXDSFLSSMWYIYQASVAKKYPAVT 405
>AI759932 similar to GP|10177640|dbj contains similarity to
heparanase~gene_id:MGG23.2 {Arabidopsis thaliana},
partial (6%)
Length = 109
Score = 26.2 bits (56), Expect = 9.7
Identities = 11/16 (68%), Positives = 13/16 (80%)
Frame = +2
Query: 169 SATYSTKTYCRQTLIG 184
SA Y T+TY RQ+LIG
Sbjct: 2 SAAYDTRTYRRQSLIG 49
>BU550562
Length = 657
Score = 26.2 bits (56), Expect = 9.7
Identities = 13/41 (31%), Positives = 21/41 (50%), Gaps = 1/41 (2%)
Frame = -1
Query: 7 YTVSKNYTIHGWELGNELC-GKGIGISISPYQYANDATILR 46
+T+ + T+ NE C KG+ I SPYQ+ ++R
Sbjct: 300 WTLKRGKTLDNKS*KNEPCLSKGVAIGPSPYQFIKIVVLMR 178
>TC206671 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like
protein (At5g07010), partial (40%)
Length = 1308
Score = 26.2 bits (56), Expect = 9.7
Identities = 11/38 (28%), Positives = 18/38 (46%), Gaps = 1/38 (2%)
Frame = -2
Query: 110 PTYLD-GVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWN 146
PTY++ G L ++ + T K+W+ G WN
Sbjct: 569 PTYINYSTIGLLDGLGQCMEWNMCTKKSWLSHVG*VWN 456
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.137 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,695,176
Number of Sequences: 63676
Number of extensions: 106478
Number of successful extensions: 516
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 512
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 514
length of query: 190
length of database: 12,639,632
effective HSP length: 92
effective length of query: 98
effective length of database: 6,781,440
effective search space: 664581120
effective search space used: 664581120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144477.14