BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.10 - phase: 0 /pseudo
(200 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215881 homologue to UP|Q8CBU4 (Q8CBU4) Mus musculus adult male... 325 9e-90
TC215882 similar to UP|Q8H1U3 (Q8H1U3) CDC26, partial (57%) 322 7e-89
CD408879 similar to GP|27351994|dbj blr3734 {Bradyrhizobium japo... 189 6e-49
CD401611 weakly similar to GP|15010776|gb| At2g11890/F23M2.5 {Ar... 145 9e-36
TC226776 similar to GB|AAP68287.1|31711862|BT008848 At1g02720 {A... 32 0.15
TC217330 similar to UP|Q9FH92 (Q9FH92) Emb|CAB62301.1 (At5g67210... 28 2.2
TC205150 homologue to UP|ADHX_ORYSA (P93436) Alcohol dehydrogena... 28 2.2
TC205151 similar to UP|ADHX_ORYSA (P93436) Alcohol dehydrogenase... 28 2.8
TC215244 similar to UP|Q9SQL0 (Q9SQL0) Jasmonic acid 2, partial ... 28 2.8
TC216000 similar to UP|O22253 (O22253) Photolyase/blue-light rec... 28 3.7
TC215249 similar to GB|BAB20599.1|12060424|AB049070 AtNAC3 {Arab... 28 3.7
TC226446 homologue to UP|P2A_MEDSA (Q06009) Serine/threonine pro... 27 4.8
TC204381 similar to UP|CRTC_RICCO (P93508) Calreticulin precurso... 27 4.8
TC205149 similar to UP|ADHX_ORYSA (P93436) Alcohol dehydrogenase... 27 4.8
AW311192 weakly similar to SP|P93508|CRTC_ Calreticulin precurso... 27 6.3
CK606263 27 8.2
>TC215881 homologue to UP|Q8CBU4 (Q8CBU4) Mus musculus adult male urinary
bladder cDNA, RIKEN full-length enriched library,
clone:9530007N19 product:villin 2, full insert sequence.
(Fragment), partial (5%)
Length = 1049
Score = 325 bits (833), Expect = 9e-90
Identities = 162/200 (81%), Positives = 179/200 (89%)
Frame = +1
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 60
MEVEVKLRL +A+++ VTTLLSPFHVITHRQ NLFFDGA SELS+RRA+LRLRFYGDDE
Sbjct: 250 MEVEVKLRLADANAHRHVTTLLSPFHVITHRQQNLFFDGAASELSARRAVLRLRFYGDDE 429
Query: 61 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVG 120
RCV SLKA+AVLVDGVSRVEEDEEDLDP IGR+CV EP KLG VESR++ RV+EEFG
Sbjct: 430 RCVASLKARAVLVDGVSRVEEDEEDLDPSIGRECVAEPAKLGSVESRVLKRVQEEFGT-- 603
Query: 121 ENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKEN 180
E G VGLGGF N+R+VYDWKGLKLEVDET FDFGTL+EIECESSDPE AKR+LEEFLKEN
Sbjct: 604 EKGLVGLGGFGNLRSVYDWKGLKLEVDETSFDFGTLYEIECESSDPERAKRLLEEFLKEN 783
Query: 181 GIDYSYSVASKFAIFRAGKL 200
I+YSYS ASKFAIFR+GKL
Sbjct: 784 EINYSYSTASKFAIFRSGKL 843
>TC215882 similar to UP|Q8H1U3 (Q8H1U3) CDC26, partial (57%)
Length = 1128
Score = 322 bits (825), Expect = 7e-89
Identities = 160/200 (80%), Positives = 178/200 (89%)
Frame = +1
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 60
MEVEVKL L +++++ VTTLLSPFHV+THRQ NLFFDGA SELS+RRA+LRLRFYGDDE
Sbjct: 304 MEVEVKLCLADSNAHRHVTTLLSPFHVVTHRQQNLFFDGAASELSARRAVLRLRFYGDDE 483
Query: 61 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVG 120
RCV SLKA+AVLVDGVSRVEEDEEDLDP+IGR CV EP KLG VESR++GR KEEFG
Sbjct: 484 RCVASLKARAVLVDGVSRVEEDEEDLDPRIGRACVAEPAKLGSVESRVLGRAKEEFGT-- 657
Query: 121 ENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKEN 180
E G VGLGGF N+R+VYDWKGLKLEVDET FDFGTL+EIECESSDPE AKR+LEEFLKEN
Sbjct: 658 EKGLVGLGGFGNLRSVYDWKGLKLEVDETKFDFGTLYEIECESSDPEGAKRLLEEFLKEN 837
Query: 181 GIDYSYSVASKFAIFRAGKL 200
I+YSYS ASKFAIFR+GKL
Sbjct: 838 EINYSYSTASKFAIFRSGKL 897
>CD408879 similar to GP|27351994|dbj blr3734 {Bradyrhizobium japonicum USDA
110}, partial (5%)
Length = 626
Score = 189 bits (481), Expect = 6e-49
Identities = 97/168 (57%), Positives = 128/168 (75%), Gaps = 1/168 (0%)
Frame = +1
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 60
MEVE+KLRLP++ ++ +++ LL+PFH T Q N+FFDG EL+S A+LR+RFY D E
Sbjct: 121 MEVEIKLRLPDSATHQKLSNLLAPFHTKTLIQENIFFDGMNKELTSNLAVLRIRFY-DLE 297
Query: 61 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVES-RIMGRVKEEFGVV 119
CV+SLKAK V+ G+SR+EE EE D +GR C+ EP +L VES I+ RV++E+ V
Sbjct: 298 HCVLSLKAKPVISAGISRMEEHEEPFDVALGRACIAEPWRLLSVESSEILKRVRDEYRV- 474
Query: 120 GENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPE 167
GENG V LGGF+NVR V+ WKGLKLE+DET++DFGT +E+ECES DPE
Sbjct: 475 GENGVVCLGGFRNVRAVHQWKGLKLELDETNYDFGTNYELECESDDPE 618
>CD401611 weakly similar to GP|15010776|gb| At2g11890/F23M2.5 {Arabidopsis
thaliana}, partial (37%)
Length = 562
Score = 145 bits (367), Expect = 9e-36
Identities = 72/88 (81%), Positives = 78/88 (87%)
Frame = -2
Query: 113 KEEFGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRI 172
KEEFG E G VGLGGF N+R+VYDWKGLKLEVDET FDFGTL+EIECESSDPE AKR+
Sbjct: 561 KEEFGT--EKGLVGLGGFGNLRSVYDWKGLKLEVDETKFDFGTLYEIECESSDPEGAKRL 388
Query: 173 LEEFLKENGIDYSYSVASKFAIFRAGKL 200
LEEFLKEN I+YSYS ASKFAIFR+GKL
Sbjct: 387 LEEFLKENEINYSYSTASKFAIFRSGKL 304
>TC226776 similar to GB|AAP68287.1|31711862|BT008848 At1g02720 {Arabidopsis
thaliana;} , partial (68%)
Length = 1307
Score = 32.3 bits (72), Expect = 0.15
Identities = 30/93 (32%), Positives = 40/93 (42%)
Frame = -3
Query: 56 YGDDERCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEE 115
+GD+E L+A VL DGV + E LD + G VDE G V +
Sbjct: 807 FGDEEVEEDVLRALGVLEDGVDGGDGAAEVLDVE-GDGDVDEGG------------VADG 667
Query: 116 FGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDE 148
G G FVG+GG R + K + E +E
Sbjct: 666 GGDGGGGAFVGVGGIAETRGGAEGKARRREAEE 568
>TC217330 similar to UP|Q9FH92 (Q9FH92) Emb|CAB62301.1 (At5g67210), partial
(87%)
Length = 1091
Score = 28.5 bits (62), Expect = 2.2
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +2
Query: 6 KLRLPNADSYHRVTTLLSPFHVITHRQHNLFF 37
KL+ P A S H + + P H H++HNL F
Sbjct: 80 KLQSPMAFSLHILLYISLPCHPYLHKRHNLIF 175
>TC205150 homologue to UP|ADHX_ORYSA (P93436) Alcohol dehydrogenase class III
(Glutathione-dependent formaldehyde dehydrogenase)
(FDH) (FALDH) (GSH-FDH) , partial (38%)
Length = 441
Score = 28.5 bits (62), Expect = 2.2
Identities = 17/78 (21%), Positives = 36/78 (45%), Gaps = 3/78 (3%)
Frame = +3
Query: 123 GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFL---KE 179
G VGL + + + + +++D FD F + E +P E + +++ + +
Sbjct: 138 GTVGLAVAEGAKTAGASRVIGIDIDSKKFDVAKNFGVT-EFINPNEHDKPIQQVIIDRTD 314
Query: 180 NGIDYSYSVASKFAIFRA 197
G+DYS+ ++ RA
Sbjct: 315 GGVDYSFECIGNVSVMRA 368
>TC205151 similar to UP|ADHX_ORYSA (P93436) Alcohol dehydrogenase class III
(Glutathione-dependent formaldehyde dehydrogenase)
(FDH) (FALDH) (GSH-FDH) , partial (54%)
Length = 667
Score = 28.1 bits (61), Expect = 2.8
Identities = 17/78 (21%), Positives = 36/78 (45%), Gaps = 3/78 (3%)
Frame = +2
Query: 123 GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFL---KE 179
G VGL + + + + +++D FD F + E +P E + +++ + +
Sbjct: 308 GTVGLAVAQGAKTAGASRVIGIDIDSKKFDIAKNFGVT-EFINPYEHDKPIQQVIIDRTD 484
Query: 180 NGIDYSYSVASKFAIFRA 197
G+DYS+ ++ RA
Sbjct: 485 GGVDYSFECIGNVSVMRA 538
>TC215244 similar to UP|Q9SQL0 (Q9SQL0) Jasmonic acid 2, partial (57%)
Length = 1463
Score = 28.1 bits (61), Expect = 2.8
Identities = 18/36 (50%), Positives = 20/36 (55%)
Frame = -3
Query: 100 KLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNVRN 135
KLG S + GRV+E V GF LGGF NV N
Sbjct: 531 KLGA--SVLSGRVEEAILVHDPIGFGALGGFANVEN 430
>TC216000 similar to UP|O22253 (O22253) Photolyase/blue-light receptor
(Photolyase/blue light photoreceptor PHR2), partial
(84%)
Length = 1641
Score = 27.7 bits (60), Expect = 3.7
Identities = 24/75 (32%), Positives = 32/75 (42%), Gaps = 8/75 (10%)
Frame = -3
Query: 63 VVSLKAKAVLVDGVSRVE-EDEEDLDPKIGR-------DCVDEPGKLGLVESRIMGRVKE 114
+V + +V GV V+ ED E + G + V EP G E +GRV
Sbjct: 403 LVEAGCRLAVVGGVEAVDGEDGEGVVVGGGEALIVEEAEVVAEPDDGGAAEGGGVGRVAG 224
Query: 115 EFGVVGENGFVGLGG 129
G GE G G+GG
Sbjct: 223 AVGSEGERGVEGVGG 179
>TC215249 similar to GB|BAB20599.1|12060424|AB049070 AtNAC3 {Arabidopsis
thaliana;} , partial (54%)
Length = 781
Score = 27.7 bits (60), Expect = 3.7
Identities = 15/30 (50%), Positives = 17/30 (56%)
Frame = -3
Query: 106 SRIMGRVKEEFGVVGENGFVGLGGFKNVRN 135
S + GRV+E V GF LGGF NV N
Sbjct: 599 SVLSGRVEEAILVHDPIGFGALGGFANVEN 510
>TC226446 homologue to UP|P2A_MEDSA (Q06009) Serine/threonine protein
phosphatase PP2A catalytic subunit , partial (80%)
Length = 1211
Score = 27.3 bits (59), Expect = 4.8
Identities = 11/29 (37%), Positives = 19/29 (64%), Gaps = 3/29 (10%)
Frame = -3
Query: 7 LRLPNADSY---HRVTTLLSPFHVITHRQ 32
LR+PN + H + +++P H+ITH+Q
Sbjct: 153 LRIPNFQCHEE*HSLN*IITPVHIITHKQ 67
>TC204381 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(81%)
Length = 1408
Score = 27.3 bits (59), Expect = 4.8
Identities = 16/47 (34%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = +1
Query: 130 FKNVRNVYDWKGLK-LEVDETHFDFGTLFEIECESSDPEEAKRILEE 175
FK+ ++Y + LK + ++ GTLF+ + DPE AK+++EE
Sbjct: 694 FKDDPDLYVFPNLKYVGIELWQVKSGTLFDNVLITDDPEYAKQLVEE 834
>TC205149 similar to UP|ADHX_ORYSA (P93436) Alcohol dehydrogenase class III
(Glutathione-dependent formaldehyde dehydrogenase)
(FDH) (FALDH) (GSH-FDH) , partial (49%)
Length = 790
Score = 27.3 bits (59), Expect = 4.8
Identities = 16/76 (21%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
Frame = +3
Query: 125 VGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFL---KENG 181
VGL + + + + +++D FD F + E +P E + +++ + + G
Sbjct: 48 VGLAVAEGAKTAGASRVIGIDIDSKKFDIAKNFGVT-EFINPNEHDKPIQQVIIDRTDGG 224
Query: 182 IDYSYSVASKFAIFRA 197
+DYS+ ++ RA
Sbjct: 225 VDYSFECIGNVSVMRA 272
>AW311192 weakly similar to SP|P93508|CRTC_ Calreticulin precursor. [Castor
bean] {Ricinus communis}, partial (23%)
Length = 310
Score = 26.9 bits (58), Expect = 6.3
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +2
Query: 146 VDETHFDFGTLFEIECESSDPEEAKRILEE 175
+D GTLF+ + DPE AK+++EE
Sbjct: 89 IDLRQVKSGTLFDNALITDDPESAKQLVEE 178
>CK606263
Length = 303
Score = 26.6 bits (57), Expect = 8.2
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 151 FDFGTLFEIECESSDPEEAKRILEEF 176
FD GTL + ESS E+ +++ EF
Sbjct: 292 FDLGTLLALHGESSTDEQGQKVEREF 215
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.141 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,657,876
Number of Sequences: 63676
Number of extensions: 97394
Number of successful extensions: 475
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 470
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of query: 200
length of database: 12,639,632
effective HSP length: 92
effective length of query: 108
effective length of database: 6,781,440
effective search space: 732395520
effective search space used: 732395520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144477.10