Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144474.7 - phase: 0 
         (329 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC213008 weakly similar to UP|Q94AA3 (Q94AA3) AT3g09210/F3L24_8,...   113  1e-25
BF219556 weakly similar to GP|15146210|gb| AT3g09210/F3L24_8 {Ar...   103  8e-23
TC216648 weakly similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, pa...    33  0.23
AW508862                                                               33  0.23
TC223428 similar to UP|Q8VZE1 (Q8VZE1) At2g17250/T23A1.11, parti...    31  0.67
TC214570 homologue to PIR|T05766|T05766 peptidylprolyl isomerase...    31  0.67
CA853170                                                               30  1.5
TC216756 similar to PIR|T05768|T05768 subtilisin-like proteinase...    30  1.5
TC206106 weakly similar to UP|Q7RK82 (Q7RK82) Maebl (Fragment), ...    29  2.6
TC216018 homologue to UP|O04720 (O04720) Cysteine proteinase inh...    29  2.6
TC216677 similar to GB|AAS58514.1|45357110|AY550303 MYB transcri...    29  2.6
TC227415 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (89%)    29  3.3
TC221939 similar to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 ...    28  4.4
CA938733 similar to PIR|T07139|T071 cysteine proteinase inhibito...    28  4.4
TC203793 similar to PIR|T02602|T02602 vacuolar sorting receptor ...    28  4.4
BM887094 similar to GP|13265463|gb| AT4g27350 {Arabidopsis thali...    28  4.4
TC228275 weakly similar to UP|Q9M3D0 (Q9M3D0) Lipase-like protei...    28  5.7
TC215938 similar to UP|Q940M0 (Q940M0) At1g63980/F22C12_9, parti...    28  5.7
TC221115 similar to UP|Q9MSB5 (Q9MSB5) Phosphoenolpyruvate/phosp...    28  7.4
TC232450 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-int...    28  7.4

>TC213008 weakly similar to UP|Q94AA3 (Q94AA3) AT3g09210/F3L24_8, partial
           (17%)
          Length = 422

 Score =  113 bits (283), Expect = 1e-25
 Identities = 64/121 (52%), Positives = 79/121 (64%), Gaps = 16/121 (13%)
 Frame = +1

Query: 10  WNPP---PRFYTPSLSTKPP----LSISATSSPEL-------LTAKERRRLRNERRE--S 53
           WNPP      + PS+S  P      ++SA+S+P         L+A+ERRR R+ERRE  S
Sbjct: 58  WNPPCFHDALHLPSVSLLPTRPFFFTLSASSTPSAPVESASQLSARERRRARSERRETRS 237

Query: 54  NATNWKEEVENKLIQKTKKVNKSWKDELNLDNLMKLGPQWWGVRVSRVKGQYTAEALARS 113
            A NW+E VE +L++K KK   SW DELNLDNL KLGPQWW +RVSRVKG   A+ LARS
Sbjct: 238 GAKNWREVVEERLMEKPKKQKGSWMDELNLDNLAKLGPQWWVIRVSRVKGNDIAQLLARS 417

Query: 114 L 114
           L
Sbjct: 418 L 420


>BF219556 weakly similar to GP|15146210|gb| AT3g09210/F3L24_8 {Arabidopsis
           thaliana}, partial (19%)
          Length = 704

 Score =  103 bits (258), Expect = 8e-23
 Identities = 53/92 (57%), Positives = 65/92 (70%)
 Frame = -2

Query: 238 DVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPKLVTGSTVQIISGSFLGFAGTLKKL 297
           D+  AIVD   KR SRK S     T+ +S+      LV GSTV+++SG+F GF GT KKL
Sbjct: 685 DILNAIVDYXSKRXSRKASXXXKATDASSTRINYKLLVPGSTVRVLSGTFSGFTGTXKKL 506

Query: 298 NSKTKMATVHFTMFGKENIVDLDVSEIVPETN 329
           N KTK+ATVHFT+FGKENI D+DV+EI  ETN
Sbjct: 505 NRKTKLATVHFTLFGKENIADIDVNEIAIETN 410


>TC216648 weakly similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (41%)
          Length = 2064

 Score = 32.7 bits (73), Expect = 0.23
 Identities = 39/154 (25%), Positives = 65/154 (41%), Gaps = 8/154 (5%)
 Frame = +1

Query: 169 EYEGVGGFIGSKVGNTKKQINKPRPVAEDDMEAIFK--QAKVEQENADK--AFEEEEKKA 224
           E    G   G   GN  +++ +    AE+  E I +  + K EQE  ++    EE+EK+ 
Sbjct: 229 ENANAGDKDGGAAGN--QEVEEEADAAEEKKEEIDEDGEEKDEQEEKEEEDEAEEKEKEK 402

Query: 225 AVNSGNPNKELESDVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPKLVTGSTVQIIS 284
           A   G   +  +  V+ A    +P R  RKT  + ++   A SA+       G  ++   
Sbjct: 403 AKGKGKRRRSKKDPVTPA-PSERPTR-ERKTVERFSVPSPAKSARSSAS--KGLIIEKGR 570

Query: 285 GSFL----GFAGTLKKLNSKTKMATVHFTMFGKE 314
           G+ L      A  L K      +  +H  +FGK+
Sbjct: 571 GTQLKDIPNVAFKLSKRKPDDNLHMLHTLLFGKK 672



 Score = 30.8 bits (68), Expect = 0.88
 Identities = 21/86 (24%), Positives = 43/86 (49%)
 Frame = +1

Query: 185  KKQINKPRPVAEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKAIV 244
            K +++  + V+++D +      + + E + ++ EEEEK  +    +     ES VSKA  
Sbjct: 1072 KAELSDAKGVSQEDEDVAVPNNESDDEES-RSEEEEEKSKSRKRTSKKAVKESSVSKADR 1248

Query: 245  DSKPKRGSRKTSNQLTITEEASSAKK 270
             S  K+   K +  +  T++  ++KK
Sbjct: 1249 TSSVKKTPVKDAKSIEKTKKKPTSKK 1326


>AW508862 
          Length = 188

 Score = 32.7 bits (73), Expect = 0.23
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +3

Query: 3  GMLTLHLWNPPPRFYTPSLSTKPPLSISATSSP 35
          G  T   W+PPP   +P+    PP S +A SSP
Sbjct: 21 GSTTTSCWSPPPMLCSPTAPPSPPPSETAPSSP 119


>TC223428 similar to UP|Q8VZE1 (Q8VZE1) At2g17250/T23A1.11, partial (22%)
          Length = 635

 Score = 31.2 bits (69), Expect = 0.67
 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
 Frame = +2

Query: 12  PPPRFYTPSLSTKPPL--SISATSSPELLTAKERRRLRNERRESNATNW 58
           PPP    PS ST P    S +++SSP   ++  RR +R  R      +W
Sbjct: 224 PPPATTNPSSSTSPGSVPSSTSSSSPSSTSSLRRREMRRSRNSCWTRSW 370


>TC214570 homologue to PIR|T05766|T05766 peptidylprolyl isomerase  M4E13.20 -
           Arabidopsis thaliana {Arabidopsis thaliana;}  , partial
           (13%)
          Length = 944

 Score = 31.2 bits (69), Expect = 0.67
 Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 3/99 (3%)
 Frame = +2

Query: 178 GSKVGNTKKQINKP-RPVAEDDMEAIFKQAKVEQENADKAFEEEE--KKAAVNSGNPNKE 234
           GS +  T   +  P + V  D++E + KQ KV+ +  D+   E E  KK        NKE
Sbjct: 344 GSGILTTDSPVAVPAKKVKVDEVEEVEKQEKVKVKKVDEETVEVEVEKKEKKKKKKKNKE 523

Query: 235 LESDVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPK 273
                S     ++ K+   K   + +  E   S KKK K
Sbjct: 524 NSEAASSDEEKTEKKKKKHKDKVEDSSPELDKSEKKKKK 640


>CA853170 
          Length = 493

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 13/46 (28%), Positives = 24/46 (51%)
 Frame = -1

Query: 65  KLIQKTKKVNKSWKDELNLDNLMKLGPQWWGVRVSRVKGQYTAEAL 110
           K  +++ K+   +K E++L  +++ GP+WW     R  G    E L
Sbjct: 331 KYKRESNKIEIGFKQEVSLQRVVE*GPRWWFHEGRRCSGLMATEIL 194


>TC216756 similar to PIR|T05768|T05768 subtilisin-like proteinase  -
           Arabidopsis thaliana {Arabidopsis thaliana;}  , partial
           (13%)
          Length = 764

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 25/76 (32%), Positives = 35/76 (45%), Gaps = 8/76 (10%)
 Frame = -3

Query: 12  PPPRFYTPSLSTKPPLSISATSSPEL---LTAKERRRLRNERRESNA-----TNWKEEVE 63
           PPP     S+S+ PP + +AT+SP     L A  R R +   R  +      T+WK    
Sbjct: 363 PPPPSPHSSVSSPPPRTRAATASPSRSPHLRAPPRLRGKQSSRAPHCSRSV*TSWKPPSS 184

Query: 64  NKLIQKTKKVNKSWKD 79
            K  Q+TK+  K   D
Sbjct: 183 TK--QETKQQRKGNSD 142


>TC206106 weakly similar to UP|Q7RK82 (Q7RK82) Maebl (Fragment), partial (7%)
          Length = 1040

 Score = 29.3 bits (64), Expect = 2.6
 Identities = 21/93 (22%), Positives = 42/93 (44%), Gaps = 3/93 (3%)
 Frame = +3

Query: 5   LTLHLWNPPPRFYTPSLSTKPPLS---ISATSSPELLTAKERRRLRNERRESNATNWKEE 61
           L+ H W   P+        +PPL    +   +  +L   K + +L+  ++ S    W+++
Sbjct: 225 LSNHPWPRNPK-------NQPPLKKWCLFLRTRHQLPNLKLKLKLKQMQKASKKW-WRKQ 380

Query: 62  VENKLIQKTKKVNKSWKDELNLDNLMKLGPQWW 94
              +++ KT+  N   K +L L  + K   +WW
Sbjct: 381 RRPRVV*KTRFPNLKLKLKLKLKQMQKASKKWW 479


>TC216018 homologue to UP|O04720 (O04720) Cysteine proteinase inhibitor,
           complete
          Length = 1064

 Score = 29.3 bits (64), Expect = 2.6
 Identities = 11/27 (40%), Positives = 20/27 (73%)
 Frame = -2

Query: 41  KERRRLRNERRESNATNWKEEVENKLI 67
           +ERR+ R  RR+SNA+ WK + ++ ++
Sbjct: 130 EERRK*RERRRKSNASQWKSKKKSLML 50


>TC216677 similar to GB|AAS58514.1|45357110|AY550303 MYB transcription factor
           {Arabidopsis thaliana;} , partial (51%)
          Length = 1373

 Score = 29.3 bits (64), Expect = 2.6
 Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
 Frame = +3

Query: 11  NPPPRFYTP-----SLSTKPPL-SISATSSPELLTAKERRRLRNERRESNAT-NWKEEVE 63
           NPP  FY       +L  KPP  S+SA ++    +    +++R     + +  NW E   
Sbjct: 78  NPPEGFYLDPSGGMALPGKPPFASVSAAAAAANSSEDAAKKIRKPYTITKSRENWTEPEH 257

Query: 64  NKLIQKTKKVNKSWK 78
           +K ++  +  ++ WK
Sbjct: 258 DKFLEAIQLFDRDWK 302


>TC227415 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (89%)
          Length = 1748

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +2

Query: 9   LWNPPPRFYTPSLSTKPPLSISATSS 34
           +W+PPP     S S+ PPL  S+TSS
Sbjct: 236 VWDPPPGSS*SSSSSSPPLPSSSTSS 313


>TC221939 similar to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2  , partial
           (7%)
          Length = 695

 Score = 28.5 bits (62), Expect = 4.4
 Identities = 17/38 (44%), Positives = 20/38 (51%)
 Frame = +2

Query: 14  PRFYTPSLSTKPPLSISATSSPELLTAKERRRLRNERR 51
           PR Y  S+S K     +  +SPE  TAK RRR R   R
Sbjct: 533 PRTYGSSISVKQ----NRETSPENATAKRRRRRRRNER 634


>CA938733 similar to PIR|T07139|T071 cysteine proteinase inhibitor -
          soybean, partial (28%)
          Length = 408

 Score = 28.5 bits (62), Expect = 4.4
 Identities = 11/21 (52%), Positives = 16/21 (75%)
 Frame = -1

Query: 41 KERRRLRNERRESNATNWKEE 61
          +ERR+ R  RR+SNA+ WK +
Sbjct: 66 EERRK*RERRRKSNASQWKSK 4


>TC203793 similar to PIR|T02602|T02602 vacuolar sorting receptor protein
           homolog At2g14740 - Arabidopsis thaliana {Arabidopsis
           thaliana;} , partial (31%)
          Length = 711

 Score = 28.5 bits (62), Expect = 4.4
 Identities = 25/97 (25%), Positives = 44/97 (44%), Gaps = 3/97 (3%)
 Frame = +2

Query: 194 VAEDDMEAIFKQAKVEQENADKAFEE---EEKKAAVNSGNPNKELESDVSKAIVDSKPKR 250
           V +  +    K+ K  ++ AD   E    + KK     G+PN + E+ V K   D++  +
Sbjct: 134 VTDFQIRCPMKEKKYNKKCADAVIESLGLDIKKIERCMGDPNADSENPVLKEEQDAQVGK 313

Query: 251 GSRKTSNQLTITEEASSAKKKPKLVTGSTVQIISGSF 287
           GSR     L  T   ++ + + KL  G+ ++ I   F
Sbjct: 314 GSRGDVTILP-TLVVNNRQYRGKLEKGAVMKAICAGF 421


>BM887094 similar to GP|13265463|gb| AT4g27350 {Arabidopsis thaliana},
           partial (20%)
          Length = 429

 Score = 28.5 bits (62), Expect = 4.4
 Identities = 17/45 (37%), Positives = 21/45 (45%), Gaps = 5/45 (11%)
 Frame = -2

Query: 12  PPPRFYTPSLSTKPP-----LSISATSSPELLTAKERRRLRNERR 51
           PP +  TP+  T PP      S    S P+   A  RRR R+ RR
Sbjct: 290 PPVKSRTPATRTSPPPPGPRASAPPMSGPQSPRAARRRRRRHRRR 156


>TC228275 weakly similar to UP|Q9M3D0 (Q9M3D0) Lipase-like protein, partial
          (25%)
          Length = 1153

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 13/29 (44%), Positives = 18/29 (61%)
 Frame = +3

Query: 12 PPPRFYTPSLSTKPPLSISATSSPELLTA 40
          PP  F TPS ++KPP + S+ S   L T+
Sbjct: 60 PPKAFATPSPTSKPPTAKSSPSPSSLSTS 146


>TC215938 similar to UP|Q940M0 (Q940M0) At1g63980/F22C12_9, partial (60%)
          Length = 1505

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 33/159 (20%), Positives = 58/159 (35%), Gaps = 25/159 (15%)
 Frame = +3

Query: 21  LSTKPPLSISATSSPELLTAKERRRLRNERRESNATNWKEEVENKLIQKTKKVNKSWKDE 80
           + TKP + +    S    T  + R  R ER +  +    +++E  L++K      +   +
Sbjct: 330 VETKPSVPVGNEHSVSKTTRPQGRYTRRERGKLVSQYSLKDLEGILVKKGDISGSTDNSD 509

Query: 81  LNLDNLMK---------------LGPQWWGVRVSRVKGQYT-AEALAR--------SLAK 116
             LD L                 + P WWG +   V G +  AE+  +            
Sbjct: 510 GELDMLKTSEIQKFEDEGSKYPAIPPDWWGYKYGFVSGGFLGAESKKKKSMISGKAERTA 689

Query: 117 FFPDIEFKVYAPAIHEKKRLKSGSISVKSKP-LFPGCIF 154
           FF + +  +Y   + EK       + +K +P    GC F
Sbjct: 690 FFEEDQENLY-NLVQEKSTTGKQGLGIKDRPKKVAGCYF 803


>TC221115 similar to UP|Q9MSB5 (Q9MSB5) Phosphoenolpyruvate/phosphate
           translocator, partial (18%)
          Length = 515

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 12/31 (38%), Positives = 19/31 (60%)
 Frame = +3

Query: 4   MLTLHLWNPPPRFYTPSLSTKPPLSISATSS 34
           +L LHL N  P  +TPS+  +PP   +A ++
Sbjct: 189 LLILHLSNSLPHTFTPSMPEQPPCRRAAPAT 281


>TC232450 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-interacting
           protein PHIP1, partial (4%)
          Length = 481

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 18/62 (29%), Positives = 34/62 (54%), Gaps = 2/62 (3%)
 Frame = +1

Query: 13  PPRFYTPSLSTKPPLSISATSSPELLTAKERRRLRNER--RESNATNWKEEVENKLIQKT 70
           PP F   + ++   L  SAT +P     ++RR+LR+++  ++ N  N + E +    +KT
Sbjct: 136 PPSFSINNNNSLKTLLDSATPTPRFFKREKRRKLRSDQPPQQPNKEN-ETETQGSPNKKT 312

Query: 71  KK 72
           K+
Sbjct: 313 KR 318


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.311    0.129    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,828,174
Number of Sequences: 63676
Number of extensions: 168048
Number of successful extensions: 1315
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 1292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1309
length of query: 329
length of database: 12,639,632
effective HSP length: 98
effective length of query: 231
effective length of database: 6,399,384
effective search space: 1478257704
effective search space used: 1478257704
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)


Medicago: description of AC144474.7