BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144459.6 - phase: 0
(1105 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220322 96 8e-31
BM308209 58 3e-18
BF425842 81 3e-15
TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F1... 77 5e-14
TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transp... 72 2e-12
BE806360 similar to GP|6175165|gb| Mutator-like transposase {Ara... 53 6e-07
TC220753 49 9e-06
TC232149 49 1e-05
TC214000 43 9e-04
TC214082 similar to UP|Q75QC2 (Q75QC2) Glutamate-rich protein, p... 35 0.24
TC230490 similar to UP|Q8LEE4 (Q8LEE4) Zinc finger protein, part... 34 0.31
BM108247 weakly similar to PIR|H84710|H847 Mutator-like transpos... 34 0.31
TC214054 similar to UP|Q75QC2 (Q75QC2) Glutamate-rich protein, p... 34 0.41
TC207649 similar to UP|Q9SY66 (Q9SY66) F14N23.12 (At1g10240/F14N... 34 0.41
TC227468 similar to UP|Q8H1D0 (Q8H1D0) Transcription factor MYBS... 32 1.5
BI701322 similar to GP|9988432|dbj| P0671B11.14 {Oryza sativa (j... 32 1.5
TC205816 similar to UP|Q39082 (Q39082) Dihydrolipoamide acetyltr... 31 2.6
TC227415 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (89%) 31 2.6
AI960729 similar to GP|21593313|gb| protein disulfide isomerase ... 31 2.6
BE660213 similar to PIR|T10797|T10 cellulose synthase (EC 2.4.1.... 31 3.5
>TC220322
Length = 1264
Score = 95.9 bits (237), Expect(2) = 8e-31
Identities = 58/173 (33%), Positives = 87/173 (49%), Gaps = 2/173 (1%)
Frame = +2
Query: 567 AKATYYQGWLEKMNELKKIDLGAWEWLMAVEPKKWCKHALPFYSKCDVLMNXISESLNAT 626
AK+T + M LK I+ AWE+L + W K K D + N E N
Sbjct: 8 AKSTTVAEFEGHMAHLKTINCQAWEYLNKWPKQAWTKAHFSTIPKVDNICNNTCEVFNFR 187
Query: 627 ILSARDQPIISMAEWIRHYLMRRMTTSATKLQKWQHNVMPMPRKRLDKEITLAAHWRSTW 686
IL R +PII+M E IR Y+MR M KL + + KRL+KE A W W
Sbjct: 188 ILQYRCKPIITMLEEIRSYIMRTMAARKVKLSGKPGPLCLVQYKRLEKEFHFANQWTPIW 367
Query: 687 SG--IGEQFQVMHTYNRQQFIVDIAKRSCSCNFWEIVGIPCRHAVSALGKRKQ 737
G +G +++V N+ + V++A +C+C W++ G+PCRHA++ + K+
Sbjct: 368 CGDNMGLRYEVHMWGNKVE--VNLAGWTCTCGVWQLTGMPCRHAIATITLTKE 520
Score = 57.8 bits (138), Expect(2) = 8e-31
Identities = 29/94 (30%), Positives = 46/94 (48%), Gaps = 3/94 (3%)
Frame = +1
Query: 738 RPEMFVDDYYSRTKYALCYSFAISPINGMDMWPEVEAPELLPPNYKNGPGRPRKLRIREF 797
+PE ++ S Y Y I P+ G W + + +PP + GRP+K R R
Sbjct: 523 KPEDMCHEWLSIEAYNKTYQHFIEPVQGPQYWAQTQYTHPVPPYKRVQRGRPKKNRKRSV 702
Query: 798 DEN---GSRMRRQGVAYRCTKCDQFGHNQRRCKS 828
DE+ G +++R+ + C +C Q HN R CK+
Sbjct: 703 DEDNVTGHKLKRKLAEFTCGRCGQTNHNIRSCKN 804
>BM308209
Length = 435
Score = 57.8 bits (138), Expect(2) = 3e-18
Identities = 23/52 (44%), Positives = 38/52 (72%)
Frame = +2
Query: 582 LKKIDLGAWEWLMAVEPKKWCKHALPFYSKCDVLMNXISESLNATILSARDQ 633
+K+++ A++WL+ V K W KH +Y+KCDVLMN + ++ N+TIL AR++
Sbjct: 122 IKEVNNEAYDWLIKVGTKLWRKHTFSYYTKCDVLMNNLCDAFNSTILCAREK 277
Score = 53.5 bits (127), Expect(2) = 3e-18
Identities = 24/42 (57%), Positives = 32/42 (76%)
Frame = +3
Query: 543 RHLYANFKKKFGGGSQIRDLMMGAAKATYYQGWLEKMNELKK 584
RH+Y NFK KF GG+ +R+LMM AAKA++ + W EKMN K+
Sbjct: 6 RHIYNNFKNKFVGGTLLRNLMMRAAKASFEEAWDEKMNISKR 131
Score = 47.4 bits (111), Expect = 4e-05
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = +3
Query: 679 AAHWRSTWSGIGEQFQVMHTYNRQQFIVDIAKRSCSCNFWEIVGIPCRHAVSAL 732
+ +W +T SG F+V H+ +FIV++ SCS N WEI GI CRHA +A+
Sbjct: 276 SGNWFATLSG-HLVFEVSHSMLSDKFIVNLNSWSCS*NLWEITGILCRHACAAI 434
>BF425842
Length = 414
Score = 80.9 bits (198), Expect = 3e-15
Identities = 44/119 (36%), Positives = 62/119 (51%), Gaps = 5/119 (4%)
Frame = +3
Query: 440 FDGCKKGFISACRPFVGVDGCHLKTRYGGQLLIAVGRDPNDQYFPLAFGVVETECKESWK 499
F+ C +GF C+P V VDG L +Y LL A+G+D + FPLAF +VE+E KE+W
Sbjct: 45 FNPCIEGF-KYCKPLVQVDGTFLTGKYNDTLLTAIGQDGSRDNFPLAFAIVESETKEAWI 221
Query: 500 WFIQLLMEDIGQDKRYVFISDQQKGLLSVFDEMF-----DTIDHRLCLRHLYANFKKKF 553
WF+ L + ISD+ GLL+ + C+RH+ +NF K F
Sbjct: 222 WFLHYL*RYVTPQPNLCIISDRGTGLLATLQSKHVGWNGPDVSSVYCIRHIASNFNK*F 398
>TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;} , partial (6%)
Length = 814
Score = 76.6 bits (187), Expect = 5e-14
Identities = 41/128 (32%), Positives = 66/128 (51%), Gaps = 5/128 (3%)
Frame = +1
Query: 705 IVDIAKRSCSCNFWEIVGIPCRHAVSALGKRKQRPEMFVDDYYSRTKYALCYSFAISPIN 764
IVDI CSC W++ G+PC HA++ + P + Y++ Y L Y+ +I P+
Sbjct: 64 IVDIDNWDCSCKGWQLTGVPCCHAIAVFECVGRSPYDYCSRYFTVENYRLTYAESIHPVP 243
Query: 765 GMDMWPEVEAPE-----LLPPNYKNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCDQF 819
+D P V+ ++PP K PGRP+ ++ D ++RQ +C+KC
Sbjct: 244 NVDK-PPVQGESTALVMVIPPPTKRPPGRPKMKQVESID----IIKRQ---LQCSKCKGL 399
Query: 820 GHNQRRCK 827
GHN++ CK
Sbjct: 400 GHNRKTCK 423
>TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transposase
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (12%)
Length = 617
Score = 71.6 bits (174), Expect = 2e-12
Identities = 43/135 (31%), Positives = 65/135 (47%), Gaps = 7/135 (5%)
Frame = +2
Query: 705 IVDIAKRSCSCNFWEIVGIPCRHAVSALGKRKQRPEMFVDDYYSRTKYALCYSFAISPIN 764
IV+I SCSC W++ GIPC HA +AL ++ F ++ + Y+ I PI
Sbjct: 17 IVNIGSHSCSCRDWQLNGIPCSHAAAALISCRKDVYAFSQKCFTAASFRDTYAETIHPIP 196
Query: 765 GMDMWPEVEAPEL-------LPPNYKNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCD 817
G W + + PP + PGRP K R+ D N R+ C++C+
Sbjct: 197 GKLEWSKTGNSSMDDNILVVRPPKLRRPPGRPEK-RMCVDDLN-----REKHTVHCSRCN 358
Query: 818 QFGHNQRRCKSDVQN 832
Q GH +R CK+++ N
Sbjct: 359 QTGHYKRTCKAEMIN 403
>BE806360 similar to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (17%)
Length = 431
Score = 53.1 bits (126), Expect = 6e-07
Identities = 30/88 (34%), Positives = 51/88 (57%), Gaps = 4/88 (4%)
Frame = +2
Query: 486 AFGVVETECKESWKWFIQLL--MEDIGQDK--RYVFISDQQKGLLSVFDEMFDTIDHRLC 541
AFGVV+ E ++W WF+ L + +I + R +SD+QKG++ + F T H C
Sbjct: 2 AFGVVDEENDDNWMWFLSELHNLLEIHTENMLRLTILSDRQKGIVDGVEASFPTAFHGFC 181
Query: 542 LRHLYANFKKKFGGGSQIRDLMMGAAKA 569
++HL +F+K+F + + +L+ AA A
Sbjct: 182 MQHLSDSFRKEF-NNTMLVNLLWEAANA 262
>TC220753
Length = 1312
Score = 49.3 bits (116), Expect = 9e-06
Identities = 32/118 (27%), Positives = 49/118 (41%), Gaps = 4/118 (3%)
Frame = +2
Query: 713 CSCNFWEIVGIPCRHAVSALGKRKQRPEMFVDDYYSRTKYALCYSFAISPINGMDMWPEV 772
C ++ + +PC HA++A + FVD Y Y + D WP+
Sbjct: 236 CDYGQFQALRLPCSHAIAACAFCNLNYDDFVDPVYKLENIFKVYQHHFHSLGSEDTWPQY 415
Query: 773 EAPELL--PPNYKNGPGRPRKLRI-REFDEN-GSRMRRQGVAYRCTKCDQFGHNQRRC 826
P + P + GRP RI E DE+ +R++ +C+ C GHNQ C
Sbjct: 416 LGPHFMSDPSKRRQISGRPATTRIHNEMDESIPNRLK------KCSLCKTEGHNQSNC 571
>TC232149
Length = 1005
Score = 48.9 bits (115), Expect = 1e-05
Identities = 37/143 (25%), Positives = 60/143 (41%), Gaps = 32/143 (22%)
Frame = -3
Query: 635 IISMAEWIRHYLMRRMTTSATKLQKWQHNVMPMPRKRLDKEITLAAHWRSTWSG--IGEQ 692
I++M E IR Y+M +M + K+ ++PM + R++K + + W G G +
Sbjct: 427 ILNMCEDIRTYIMCKMKSDKLKMATRLRPLVPMQQSRIEKGKVESNK*TANWVGDPDGTR 248
Query: 693 FQVMHTYNRQQFIVDIAKRSCSCNFWEIVGI----------------------------- 723
F+V H R VD+ S +C W+++GI
Sbjct: 247 FKVCHYDTRLN--VDLQN*SFTCRMWQLIGIPLS*LNLSILDIMYCVTMNLMFFWFIYIG 74
Query: 724 -PCRHAVSALGKRKQRPEMFVDD 745
PCRHA+ A+ PE + DD
Sbjct: 73 MPCRHAIVAIRYNYHIPEGYSDD 5
>TC214000
Length = 659
Score = 42.7 bits (99), Expect = 9e-04
Identities = 27/92 (29%), Positives = 41/92 (44%), Gaps = 2/92 (2%)
Frame = +3
Query: 791 KLRIREFDE--NGSRMRRQGVAYRCTKCDQFGHNQRRCKSDVQNPEAAKRKRKTPRRKNT 848
+LR RE DE N ++R C +C + GHN+R CK+ +P+ AK T
Sbjct: 6 RLRRREVDED*NDHKLRGTNTTN*CWRCHKLGHNKRTCKNSPIHPKPAK----------T 155
Query: 849 STATEPMQTDPMQAEVAAATEPMQAEVEAGTE 880
E ++ Q+ V Q + EA T+
Sbjct: 156 RVEGEGAPSEEQQSHVQVGPNGTQVQTEANTK 251
>TC214082 similar to UP|Q75QC2 (Q75QC2) Glutamate-rich protein, partial (34%)
Length = 1032
Score = 34.7 bits (78), Expect = 0.24
Identities = 39/155 (25%), Positives = 60/155 (38%), Gaps = 8/155 (5%)
Frame = +1
Query: 841 KTPRRKNTSTATEPMQT-----DPMQAEVAAATEPMQAEVEAGTEPMQTEPIQTDPMQAE 895
+TP + TE ++T +P+ A A ATE AEV A E TE + A
Sbjct: 130 QTPTTVPENETTEVIKTQETTPEPVPATEAPATEQAAAEVPAQEEQQATE------VPAP 291
Query: 896 VAAGTKPMQAEVEAGTEPMQPEVEAGTQPMHTASTQDPPRHTTKIVKVKNSEAPFK---I 952
+ P + EA T T+ + T +T++ K V V+ E K
Sbjct: 292 ESTTEAPKEETTEAPT----------TETVETTTTEEAKPEEPKEVAVETEEVVTKETEE 441
Query: 953 QKVVTSEAANALPEKIVTEFYEALPESQLETSLGP 987
+K E + E++ E E ++ ET P
Sbjct: 442 EKPAAEEKSEEKTEEVKEEAEEPKETTETETESAP 546
>TC230490 similar to UP|Q8LEE4 (Q8LEE4) Zinc finger protein, partial (19%)
Length = 574
Score = 34.3 bits (77), Expect = 0.31
Identities = 18/58 (31%), Positives = 25/58 (43%)
Frame = +3
Query: 788 RPRKLRIREFDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDVQNPEAAKRKRKTPRR 845
RP++ + + M Q C C QFGHN+R C V +KR+ RR
Sbjct: 228 RPQQKNM*KVQR*ALAMEEQ*YTISCKICHQFGHNRRTCPQAVPFAVPSKRRNAASRR 401
>BM108247 weakly similar to PIR|H84710|H847 Mutator-like transposase
[imported] - Arabidopsis thaliana, partial (4%)
Length = 265
Score = 34.3 bits (77), Expect = 0.31
Identities = 23/82 (28%), Positives = 39/82 (47%)
Frame = +2
Query: 557 SQIRDLMMGAAKATYYQGWLEKMNELKKIDLGAWEWLMAVEPKKWCKHALPFYSKCDVLM 616
S++ L+ A+ AT + EKM E++++ A +WL P +W + F +
Sbjct: 8 SRLVHLLWKASHATTTIAFKEKMGEIEEVSPEAAKWLQQFXPSQWA--LVHFKGTRFGHL 181
Query: 617 NXISESLNATILSARDQPIISM 638
+ E N IL R+ PII +
Sbjct: 182 SSNIEEFNKWILDTRELPIIXV 247
>TC214054 similar to UP|Q75QC2 (Q75QC2) Glutamate-rich protein, partial (68%)
Length = 1306
Score = 33.9 bits (76), Expect = 0.41
Identities = 37/155 (23%), Positives = 58/155 (36%), Gaps = 5/155 (3%)
Frame = +1
Query: 834 EAAKRKRKTPRRKNTSTATEPMQTDPMQAEVAAATEPMQAEVEAGTEPMQTEPIQTDPMQ 893
E A++ T T+ ++ +T P+ A ATE AEV A +P P +
Sbjct: 106 EVAQQTPTTVPENETTEVSKTQETTPVTE--APATEQPAAEVPATEQPAAEAPAPESTTE 279
Query: 894 AEVAAGTKPMQAEVEAGTEPMQPEVEAGTQPMHTASTQDPPRHTTKIVKVKNSEAPFKIQ 953
A T+ VE T + PE E P+ T K + K+ E +++
Sbjct: 280 APKEETTEAPTETVEKTTTEVAPE-EPKEVPVETEEVVAKETEEEKPAEEKSEEKTEEVK 456
Query: 954 KVV-----TSEAANALPEKIVTEFYEALPESQLET 983
+ T+E +A T E P +ET
Sbjct: 457 EEAEEPKETTETESAAAAPPATTEEENKPAESVET 561
>TC207649 similar to UP|Q9SY66 (Q9SY66) F14N23.12 (At1g10240/F14N23_12),
partial (30%)
Length = 971
Score = 33.9 bits (76), Expect = 0.41
Identities = 27/90 (30%), Positives = 43/90 (47%), Gaps = 12/90 (13%)
Frame = +2
Query: 666 PMPRKRLDKEITLAAHWRSTWSGIGEQFQVMHT----------YNRQQFIVDIAKRSCSC 715
P +L +++ LAAH+ S I + F V H + Q+ I+ SCSC
Sbjct: 281 PFAFSKLQEQLVLAAHYASF--SIEDGFLVRHHTKAEGGRKVYWAPQEGII-----SCSC 439
Query: 716 NFWEIVGIPCRHAVSAL--GKRKQRPEMFV 743
+ +E GI CRH++ L G Q P+ ++
Sbjct: 440 HQFEFTGILCRHSLRVLSTGNCFQIPDRYL 529
>TC227468 similar to UP|Q8H1D0 (Q8H1D0) Transcription factor MYBS3, partial
(56%)
Length = 1404
Score = 32.0 bits (71), Expect = 1.5
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +3
Query: 803 RMRRQGVAYRCTKCDQFGHNQRRCKS 828
R RR G+ RC+ C GHN R C S
Sbjct: 36 RRRRIGMTRRCSHCTNNGHNSRTCPS 113
>BI701322 similar to GP|9988432|dbj| P0671B11.14 {Oryza sativa (japonica
cultivar-group)}, partial (12%)
Length = 421
Score = 32.0 bits (71), Expect = 1.5
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 804 MRRQGVAYRCTKCDQFGHNQRRCKS 828
+ G+A +C+ C GHN R CKS
Sbjct: 43 VENMGIARKCSYCGNLGHNARTCKS 117
>TC205816 similar to UP|Q39082 (Q39082) Dihydrolipoamide acetyltransferase (E2)
subunit of PDC (Fragment) , partial (19%)
Length = 447
Score = 31.2 bits (69), Expect = 2.6
Identities = 36/121 (29%), Positives = 54/121 (43%), Gaps = 1/121 (0%)
Frame = +2
Query: 966 EKIVTEFYEALPESQLETSLGPDVFDDLDDSVLAAITDQMFEAAEKEFNVVNVVKSRNEQ 1025
+K EF E+L E L L P+ ++ AIT + +A++ E +V S N+Q
Sbjct: 41 DKATLEF-ESLEEGYLAKILAPEGSKEVAVGHSIAITVE--DASDIEAIKNSVSSSTNQQ 211
Query: 1026 KKKTLGLKKSVKAGRKSSRLMKLKTKAIKGAGSSMDQPMVLDESEEGTLTQED-HGLIKS 1084
K G K VKA + + + K + A +D P + GTL + D IKS
Sbjct: 212 KAPQRGTKSEVKAQKNNITRISPAAKLLI-AEYGLDAPTLNATGPYGTLLKGDVLSAIKS 388
Query: 1085 G 1085
G
Sbjct: 389 G 391
>TC227415 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (89%)
Length = 1748
Score = 31.2 bits (69), Expect = 2.6
Identities = 20/97 (20%), Positives = 35/97 (35%)
Frame = -2
Query: 821 HNQRRCKSDVQNPEAAKRKRKTPRRKNTSTATEPMQTDPMQAEVAAATEPMQAEVEAGTE 880
H R C +D + ++ R R+ PR +N + +Q + EP+Q E
Sbjct: 1081 HPSRNCGADRKGIKSQLRIREKPREQNQNLKVPCLQAQHDRGRSGELEEPIQTAKRLPRE 902
Query: 881 PMQTEPIQTDPMQAEVAAGTKPMQAEVEAGTEPMQPE 917
P+ P V + + + P QP+
Sbjct: 901 PLPRAPAPLALHYVHVLSNHHYPPRQAQHQRRPQQPQ 791
>AI960729 similar to GP|21593313|gb| protein disulfide isomerase
precursor-like {Arabidopsis thaliana}, partial (6%)
Length = 438
Score = 31.2 bits (69), Expect = 2.6
Identities = 25/69 (36%), Positives = 31/69 (44%)
Frame = +2
Query: 111 PRFFDLDVGYDEEDVMVSTDDDSGKFVGFDDSEDERTTALEDGFEDVEVEAPTNGTNRVT 170
P FD D G DE DD G F GFD S +E E +DV V N T V
Sbjct: 254 PDEFDEDDGDDE--------DDFGDFAGFDHSSEEAFKEPEVDDKDVVVLKERNFTT-VV 406
Query: 171 VNNKTLRIK 179
NN+ + ++
Sbjct: 407 ENNRFVMVE 433
>BE660213 similar to PIR|T10797|T10 cellulose synthase (EC 2.4.1.-) catalytic
chain celA1 - upland cotton, partial (17%)
Length = 644
Score = 30.8 bits (68), Expect = 3.5
Identities = 14/49 (28%), Positives = 26/49 (52%)
Frame = +1
Query: 823 QRRCKSDVQNPEAAKRKRKTPRRKNTSTATEPMQTDPMQAEVAAATEPM 871
+ R +S + + K+K+ P+ +N + Q + MQ+ AAA EP+
Sbjct: 139 KNRVESWKEKDKKKKKKKSAPKAENEAPIPPEQQMEEMQSSEAAAAEPL 285
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.134 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,676,953
Number of Sequences: 63676
Number of extensions: 633116
Number of successful extensions: 3445
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 3336
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3424
length of query: 1105
length of database: 12,639,632
effective HSP length: 107
effective length of query: 998
effective length of database: 5,826,300
effective search space: 5814647400
effective search space used: 5814647400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)
Medicago: description of AC144459.6