BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144430.8 - phase: 0 /pseudo
(91 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI427114 33 0.031
TC222789 31 0.091
TC233392 31 0.12
TC221865 30 0.16
TC211809 30 0.20
CA853414 30 0.27
TC206238 weakly similar to UP|Q7PX96 (Q7PX96) AgCP12237 (Fragmen... 30 0.27
BI785148 homologue to GP|25166165|dbj murF {Wigglesworthia brevi... 29 0.35
TC205297 similar to GB|AAL09776.1|15982858|AY057536 At1g29850/F1... 29 0.45
TC205912 weakly similar to UP|O24040 (O24040) LTCOR11, partial (... 28 0.77
TC233895 weakly similar to UP|Q84VF1 (Q84VF1) Myosin heavy chain... 28 0.77
CD402471 28 0.77
AI441437 28 1.0
TC210600 similar to UP|Q51534 (Q51534) PilW protein, partial (7%) 28 1.0
AW423846 28 1.0
CO979823 27 1.3
CD394161 27 1.7
TC225075 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B p... 27 1.7
TC217952 similar to UP|Q8RVQ6 (Q8RVQ6) SEC6, partial (20%) 27 2.2
AW620606 27 2.2
>BI427114
Length = 425
Score = 32.7 bits (73), Expect = 0.031
Identities = 28/91 (30%), Positives = 33/91 (35%), Gaps = 15/91 (16%)
Frame = +3
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLW-------LAISFDN--INFFLEHNLG------WWI 57
C RC+ E H L DC W LA F N + +LE NL +W
Sbjct: 15 CARCDFLEETCLHALRDCPKVAEFWRSVIPKKLAPKFFNGDVTVWLERNLSFSEAAFFW- 191
Query: 58 IIGLDRQLPHLFLSAVWLLWMARNRACIAQE 88
P F AV LLW RN Q+
Sbjct: 192--------PTFFGIAVELLWEFRNAVMFLQD 260
>TC222789
Length = 954
Score = 31.2 bits (69), Expect = 0.091
Identities = 26/94 (27%), Positives = 43/94 (45%), Gaps = 13/94 (13%)
Frame = +2
Query: 2 LHKRGMSPTD-VCTRCNSTSEDVFHCLLDCAFPRNLWL-AISFDNIN---------FFLE 50
LH+R + D C C + ED H C+ +W ++S+ NI+ FL+
Sbjct: 443 LHRRQIQVMDRSCPFCRNVEEDAGHLFFHCSKIIPIWWESLSWVNISGALPKDPRQHFLQ 622
Query: 51 HNLGWWIIIGLDR--QLPHLFLSAVWLLWMARNR 82
H+L I+ G R + +L+ W +W RN+
Sbjct: 623 HDL---IMAGGIRTTRWKCWWLAVTWTIWQQRNK 715
>TC233392
Length = 1145
Score = 30.8 bits (68), Expect = 0.12
Identities = 28/104 (26%), Positives = 37/104 (34%), Gaps = 23/104 (22%)
Frame = -1
Query: 2 LHKRGMSPTD-VCTRCNSTSEDVFHCLLDCAFPRNLW---------LAIS-FDNINFFLE 50
LH+R + D +C C ED H L+C R LW L +S + FL+
Sbjct: 464 LHRRQVVLEDSLCPFCRIREEDASHIFLECNKIRPLWWESQTWVRSLGVSPINKRQHFLQ 285
Query: 51 HNLG------------WWIIIGLDRQLPHLFLSAVWLLWMARNR 82
H G WWI + W LW RN+
Sbjct: 284 HVHGTPGSKRYNRWKTWWIAL-------------TWSLWKHRNQ 192
>TC221865
Length = 1045
Score = 30.4 bits (67), Expect = 0.16
Identities = 20/90 (22%), Positives = 39/90 (43%), Gaps = 12/90 (13%)
Frame = -3
Query: 12 VCTRCNSTSEDVFHCLLDCAFPRNLWL----------AISFDNINFFLEHNLGWWIIIGL 61
+C C + E+ H C + +W A S + + FL+H+ G ++GL
Sbjct: 443 LCLFCRNYEEEASHLFFSCTKTQPVWWESLSWINTSGAFSANPRHHFLQHSFG---LLGL 273
Query: 62 DR--QLPHLFLSAVWLLWMARNRACIAQEP 89
+ + +++ + +W RNR + EP
Sbjct: 272 KQYNR*KCWWVALTFTIWQHRNRTIFSNEP 183
>TC211809
Length = 895
Score = 30.0 bits (66), Expect = 0.20
Identities = 21/80 (26%), Positives = 32/80 (39%), Gaps = 11/80 (13%)
Frame = +2
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLWL----------AISFDNINFFLEH-NLGWWIIIGL 61
C CNS+ ED H C LW A + + FL+H N +
Sbjct: 398 CPLCNSSEEDAAHLFFHCTKTEXLWWETQSWVNSLGAFPHNPKDHFLQHDNRSAGGVXA- 574
Query: 62 DRQLPHLFLSAVWLLWMARN 81
R+ L+++ W++W RN
Sbjct: 575 -RRWKCLWVALTWVIWKHRN 631
>CA853414
Length = 460
Score = 29.6 bits (65), Expect = 0.27
Identities = 26/90 (28%), Positives = 40/90 (43%), Gaps = 13/90 (14%)
Frame = -2
Query: 12 VCTRCNSTSEDVFHCLLDCAFPRNL------WLAISFDNIN-FFLEHNLGWWIIIGLDRQ 64
VC E V H L C N+ W++++F +N LE + +W I GL R
Sbjct: 309 VCPFRQLQPESVQHLFLSCGVVSNIWNKCYSWISLNFGTVNPAMLEGH--FWHITGLGRS 136
Query: 65 LPHLFL-SAVW-----LLWMARNRACIAQE 88
L + ++W +W RN+ CI Q+
Sbjct: 135 KYELEVWKSIWSNVLLCIWNKRNK-CIFQQ 49
>TC206238 weakly similar to UP|Q7PX96 (Q7PX96) AgCP12237 (Fragment), partial
(9%)
Length = 1259
Score = 29.6 bits (65), Expect = 0.27
Identities = 29/92 (31%), Positives = 33/92 (35%), Gaps = 15/92 (16%)
Frame = +3
Query: 5 RGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW-------LAISFDN--INFFLEHNLG- 54
R S C RC E H L DC W LA F N + +LE NL
Sbjct: 201 RHTSMDSSCPRCPELEETCLHALRDCPKVAAFWRSVLPKKLAPKFFNGDVAVWLETNLSF 380
Query: 55 -----WWIIIGLDRQLPHLFLSAVWLLWMARN 81
+W P F AV LLW +RN
Sbjct: 381 SEAAFFW---------PTFFGIAVELLWESRN 449
>BI785148 homologue to GP|25166165|dbj murF {Wigglesworthia brevipalpis},
partial (2%)
Length = 422
Score = 29.3 bits (64), Expect = 0.35
Identities = 11/38 (28%), Positives = 17/38 (43%)
Frame = -2
Query: 5 RGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISF 42
R + + C++ ED HCL D + +WL F
Sbjct: 346 RNLVNSSAFPHCSNPREDSLHCLRDFPHAKEIWLGFGF 233
>TC205297 similar to GB|AAL09776.1|15982858|AY057536 At1g29850/F1N18_19
{Arabidopsis thaliana;} , partial (92%)
Length = 667
Score = 28.9 bits (63), Expect = 0.45
Identities = 18/56 (32%), Positives = 28/56 (49%), Gaps = 3/56 (5%)
Frame = -1
Query: 12 VCTRCNST---SEDVFHCLLDCAFPRNLWLAISFDNINFFLEHNLGWWIIIGLDRQ 64
+C R +++ S F C C FP WLAIS N+ + N G I +G+ ++
Sbjct: 244 ICLRSSASLFASS*AFSCSGFC*FPTPPWLAISSCNLLCLIASNSGSAIWLGVQKK 77
>TC205912 weakly similar to UP|O24040 (O24040) LTCOR11, partial (61%)
Length = 957
Score = 28.1 bits (61), Expect = 0.77
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = -1
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLWLAISF 42
CTR S HC L C RNL LA++F
Sbjct: 597 CTRLASNG----HCTLSCTILRNLCLALAF 520
>TC233895 weakly similar to UP|Q84VF1 (Q84VF1) Myosin heavy chain-like
protein (Fragment), partial (6%)
Length = 761
Score = 28.1 bits (61), Expect = 0.77
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +2
Query: 25 HCLLDCAFPRNLWLAISFDNINFFLEHNLGWWIIIGLDRQLPHLFLSAVWLLWMARNRA 83
H + + RNL + F+ + + L NL W + +Q+ + VWLLW + R+
Sbjct: 116 HAIAFKGYGRNLLSILDFNCLGWSLMFNLHWI*KLIWIKQMK*INSKTVWLLWRLK*RS 292
>CD402471
Length = 655
Score = 28.1 bits (61), Expect = 0.77
Identities = 18/66 (27%), Positives = 27/66 (40%), Gaps = 10/66 (15%)
Frame = -1
Query: 25 HCLLDCAFPRNLWLAI----------SFDNINFFLEHNLGWWIIIGLDRQLPHLFLSAVW 74
H L DC ++WL + S N+ + + W + D + LF + VW
Sbjct: 655 HVLRDCPRAASVWLPLVAPQYQRGFFSCSLCNWMVTYLQQPWSSVNSDDEDCVLFAATVW 476
Query: 75 LLWMAR 80
LLW R
Sbjct: 475 LLWKDR 458
>AI441437
Length = 375
Score = 27.7 bits (60), Expect = 1.0
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +2
Query: 53 LGWWIIIGLDRQLPHLFLSAVWLLWMARNR 82
+ WW +G D + L A WLLW R
Sbjct: 74 VSWWNRVGSDEKPALLLHQADWLLWFLHGR 163
>TC210600 similar to UP|Q51534 (Q51534) PilW protein, partial (7%)
Length = 1093
Score = 27.7 bits (60), Expect = 1.0
Identities = 20/91 (21%), Positives = 28/91 (29%), Gaps = 22/91 (24%)
Frame = +3
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLWL----------AISFDNINFFLEHNLG-------- 54
C CNS ED H C LW A + + FL+H+
Sbjct: 579 CPLCNSLEEDAAHLFFPCTKTLPLWWETQSWINSLGAFPHNPKDHFLQHDNSSADGVRAR 758
Query: 55 ----WWIIIGLDRQLPHLFLSAVWLLWMARN 81
WW+ + W++W RN
Sbjct: 759 RWKCWWVAL-------------TWVIWKHRN 812
>AW423846
Length = 385
Score = 27.7 bits (60), Expect = 1.0
Identities = 27/97 (27%), Positives = 39/97 (39%), Gaps = 10/97 (10%)
Frame = +3
Query: 2 LHKRGMSPTDV-CTRCNSTSEDVFHCLLDCAFPRNLWLA-ISFDNINF-FLE----HNLG 54
LH+R + D+ C C + ED H C+ LW A +S+ N F E H L
Sbjct: 78 LHRRHVEIHDLTCPFCKNKEEDAAHLFFTCSKIMPLWWASLSWTNTTAPFPENPHMHFLQ 257
Query: 55 WWIIIGLDRQLPHL---FLSAVWLLWMARNRACIAQE 88
+ G D + S W +W RN+ +E
Sbjct: 258 QVLRNGKDTSYQKWQCWWTSLTWSIWQHRNKIVXEEE 368
>CO979823
Length = 853
Score = 27.3 bits (59), Expect = 1.3
Identities = 21/82 (25%), Positives = 36/82 (43%), Gaps = 11/82 (13%)
Frame = -2
Query: 12 VCTRCNSTSEDVFHCLLDCAFPRNLWL-AISFDNI---------NFFLEHNLGWWIIIGL 61
VC CN+ E H +C LW ++S+ N+ FL++ G I GL
Sbjct: 477 VCPLCNNIEEGAAHLFFNCTKTLPLWWESMSWVNLKTAMPQTPRQHFLQY--GTDIADGL 304
Query: 62 -DRQLPHLFLSAVWLLWMARNR 82
++ +++ W +W RN+
Sbjct: 303 KSKRWKCWWIALTWTIWQHRNK 238
>CD394161
Length = 531
Score = 26.9 bits (58), Expect = 1.7
Identities = 9/25 (36%), Positives = 12/25 (48%)
Frame = -1
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLW 37
C C + EDV H +C + LW
Sbjct: 519 CPLCGTALEDVAHLFFNCNLTKGLW 445
>TC225075 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (87%)
Length = 1744
Score = 26.9 bits (58), Expect = 1.7
Identities = 10/34 (29%), Positives = 16/34 (46%)
Frame = -2
Query: 12 VCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNI 45
V C+ +EDV +C DCA L + + +
Sbjct: 1101 VLEHCSGVTEDVINCAADCAVSEELAHCVGVETV 1000
>TC217952 similar to UP|Q8RVQ6 (Q8RVQ6) SEC6, partial (20%)
Length = 1013
Score = 26.6 bits (57), Expect = 2.2
Identities = 9/27 (33%), Positives = 20/27 (73%)
Frame = +1
Query: 45 INFFLEHNLGWWIIIGLDRQLPHLFLS 71
+NFFL++ L W+ ++ L ++L +L ++
Sbjct: 55 LNFFLKYELAWFYLMLLAKKLTYLLVA 135
>AW620606
Length = 420
Score = 26.6 bits (57), Expect = 2.2
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = +1
Query: 8 SPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFFL 49
S + + C+ +D+ HCL DC R + + F + FL
Sbjct: 67 SSSSAFSPCS*NDQDIIHCLWDCPKAREV*SCLGFLSPKAFL 192
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.331 0.141 0.509
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,968,049
Number of Sequences: 63676
Number of extensions: 123545
Number of successful extensions: 811
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 807
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 811
length of query: 91
length of database: 12,639,632
effective HSP length: 67
effective length of query: 24
effective length of database: 8,373,340
effective search space: 200960160
effective search space used: 200960160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144430.8