BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144430.5 - phase: 0 /pseudo
(125 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227825 similar to UP|Q8H960 (Q8H960) Tobamovirus multiplicatio... 163 2e-41
TC227826 weakly similar to UP|Q8H960 (Q8H960) Tobamovirus multip... 150 2e-37
TC227827 similar to UP|Q8H960 (Q8H960) Tobamovirus multiplicatio... 112 4e-26
BE800618 67 2e-12
CO980799 44 3e-12
TC228044 similar to UP|Q7XHV1 (Q7XHV1) CHP-rich zinc finger prot... 34 0.020
TC216207 similar to GB|AAN72183.1|25084133|BT002172 protein kina... 31 0.13
BG790687 similar to GP|18461212|dbj P0529E05.25 {Oryza sativa (j... 30 0.22
BQ133851 29 0.48
BF423807 similar to GP|10444370|gb|A 60S ribosomal protein L10A ... 28 0.82
BF068736 similar to GP|3549691|emb thaumatin-like protein PR-5b ... 28 1.1
TC224312 28 1.4
AW598757 similar to GP|2982431|emb leucine rich repeat-like prot... 27 1.8
TC207380 27 1.8
TC228128 similar to UP|Q9ZPL9 (Q9ZPL9) Nodule-enhanced protein p... 27 2.4
TC224754 similar to UP|Q94EU9 (Q94EU9) Beta-amylase PCT-BMYI , ... 27 3.1
TC232616 similar to GB|AAD25756.1|4587525|T5I8 Contains the PF|0... 27 3.1
TC215180 homologue to UP|Q8LP17 (Q8LP17) Nine-cis-epoxycarotenoi... 26 4.1
TC211142 similar to GB|AAB68983.1|500656|YSCH9666 Yhr143wp {Sacc... 26 4.1
TC225899 similar to UP|O48685 (O48685) Pyruvate dehydrogenase E1... 26 5.3
>TC227825 similar to UP|Q8H960 (Q8H960) Tobamovirus multiplication 2B,
partial (80%)
Length = 799
Score = 163 bits (413), Expect = 2e-41
Identities = 86/113 (76%), Positives = 97/113 (85%), Gaps = 1/113 (0%)
Frame = -2
Query: 14 AANRRRPLWRPMSTQ-NAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVK 72
A N + WR M+ ++A GG +AKA VAEQISQ VQSTSNLLHLM+HSSPAQA+LVK
Sbjct: 795 ATNGQASFWRRMAAPGSSAGGGGGTAKATVAEQISQAVQSTSNLLHLMRHSSPAQAQLVK 616
Query: 73 LPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNVPQLKTVVQLLANM 125
LPKNLLAKVST+KNT+QVLEQLP VISSLDAHMENGLQNVP LKTVVQ+L+NM
Sbjct: 615 LPKNLLAKVSTIKNTEQVLEQLPGVISSLDAHMENGLQNVPHLKTVVQVLSNM 457
>TC227826 weakly similar to UP|Q8H960 (Q8H960) Tobamovirus multiplication 2B,
partial (80%)
Length = 843
Score = 150 bits (378), Expect = 2e-37
Identities = 78/104 (75%), Positives = 87/104 (83%)
Frame = +3
Query: 22 WRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKV 81
WR M+ + GG +AKA VA+QISQ VQSTSNLL LMQHSS AQA+LVKLPKNLL KV
Sbjct: 9 WRRMAAPGSDGGGGGTAKATVAKQISQVVQSTSNLLQLMQHSSLAQAQLVKLPKNLLTKV 188
Query: 82 STVKNTQQVLEQLPRVISSLDAHMENGLQNVPQLKTVVQLLANM 125
T+KNT+QVLEQLP VISSLDAHMENGL NVP LKTVVQ+L+NM
Sbjct: 189 PTIKNTEQVLEQLPGVISSLDAHMENGLPNVPHLKTVVQVLSNM 320
>TC227827 similar to UP|Q8H960 (Q8H960) Tobamovirus multiplication 2B,
partial (50%)
Length = 825
Score = 112 bits (280), Expect = 4e-26
Identities = 56/63 (88%), Positives = 61/63 (95%)
Frame = +2
Query: 48 QTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMEN 107
+ VQSTSNLLHLM+HSSPAQA+LVKLPKNLLAKVST+KNT+QVLEQLP VISSLDAHMEN
Sbjct: 5 EAVQSTSNLLHLMRHSSPAQAQLVKLPKNLLAKVSTIKNTEQVLEQLPGVISSLDAHMEN 184
Query: 108 GLQ 110
GLQ
Sbjct: 185 GLQ 193
Score = 26.2 bits (56), Expect = 4.1
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = +1
Query: 111 NVPQLKTVVQLLANM 125
+VP LKTVVQ+L+NM
Sbjct: 436 SVPHLKTVVQVLSNM 480
>BE800618
Length = 414
Score = 67.0 bits (162), Expect = 2e-12
Identities = 37/68 (54%), Positives = 46/68 (67%), Gaps = 2/68 (2%)
Frame = +2
Query: 2 KKMEQRRQDPP--TAANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHL 59
+KM++ +DP T AN + WR M+ + GG +AKA VA+QISQ VQSTSNLL L
Sbjct: 41 RKMDRGWKDPTLFTTANGQASFWRRMAAPGSDGGGGGTAKATVAKQISQVVQSTSNLLQL 220
Query: 60 MQHSSPAQ 67
MQHSS AQ
Sbjct: 221 MQHSSLAQ 244
>CO980799
Length = 887
Score = 43.5 bits (101), Expect(2) = 3e-12
Identities = 21/22 (95%), Positives = 21/22 (95%)
Frame = -2
Query: 89 QVLEQLPRVISSLDAHMENGLQ 110
QVLEQLP VISSLDAHMENGLQ
Sbjct: 514 QVLEQLPGVISSLDAHMENGLQ 449
Score = 43.1 bits (100), Expect(2) = 3e-12
Identities = 20/25 (80%), Positives = 23/25 (92%)
Frame = -3
Query: 67 QAKLVKLPKNLLAKVSTVKNTQQVL 91
QA+LVKLPKNLL KV T+KNT+QVL
Sbjct: 675 QAQLVKLPKNLLTKVPTIKNTEQVL 601
Score = 26.2 bits (56), Expect = 4.1
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -1
Query: 111 NVPQLKTVVQLLANM 125
+VP LKTVVQ+L+NM
Sbjct: 218 SVPHLKTVVQVLSNM 174
>TC228044 similar to UP|Q7XHV1 (Q7XHV1) CHP-rich zinc finger protein-like,
partial (37%)
Length = 605
Score = 33.9 bits (76), Expect = 0.020
Identities = 15/38 (39%), Positives = 21/38 (54%)
Frame = +2
Query: 7 RRQDPPTAANRRRPLWRPMSTQNAATGGDNSAKAVVAE 44
RR+DPP A+ RPL R + + + GG + V AE
Sbjct: 368 RRRDPPARASHERPLRRGLGLRGSERGGGRRRRGVAAE 481
>TC216207 similar to GB|AAN72183.1|25084133|BT002172 protein kinase-like
protein {Arabidopsis thaliana;} , partial (77%)
Length = 1503
Score = 31.2 bits (69), Expect = 0.13
Identities = 22/86 (25%), Positives = 43/86 (49%), Gaps = 2/86 (2%)
Frame = +2
Query: 7 RRQDPPTAANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPA 66
+RQ PT ++R W NA+ S+ A ++ + +STS + + SSP+
Sbjct: 44 KRQAAPTLSSRTFQTW------NASLAASTSSAA--RSEVDPSAKSTSRRILIPSRSSPS 199
Query: 67 QAKLVK--LPKNLLAKVSTVKNTQQV 90
++++VK +P + ST+ ++V
Sbjct: 200 RSRIVKQNIPNCFTRQSSTIFFKEEV 277
>BG790687 similar to GP|18461212|dbj P0529E05.25 {Oryza sativa (japonica
cultivar-group)}, partial (6%)
Length = 414
Score = 30.4 bits (67), Expect = 0.22
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +3
Query: 6 QRRQDPPTAANRRRPLWRPMSTQNAA 31
+RR DPP+ R R WRP S + +A
Sbjct: 303 RRRHDPPSLCRRGRVPWRPFSMEGSA 380
>BQ133851
Length = 367
Score = 29.3 bits (64), Expect = 0.48
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +3
Query: 5 EQRRQDPPTAANRRRPLWRPMSTQNAA 31
++ + DP ++N P W P +T+NAA
Sbjct: 84 KRSKSDPNPSSNENNPPWTPFATENAA 164
>BF423807 similar to GP|10444370|gb|A 60S ribosomal protein L10A {Phaseolus
coccineus}, partial (90%)
Length = 373
Score = 28.5 bits (62), Expect = 0.82
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = +2
Query: 72 KLPKNLLAKVSTVKNTQQVLEQLPRVIS 99
K+ KNL K TV ++ ++EQ+PR++S
Sbjct: 287 KVIKNLARKYHTVLASEGIIEQIPRLVS 370
>BF068736 similar to GP|3549691|emb thaumatin-like protein PR-5b {Cicer
arietinum}, partial (47%)
Length = 457
Score = 28.1 bits (61), Expect = 1.1
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +1
Query: 3 KMEQRRQDPPTAANRRRPLWRPMS 26
K+ R+Q PP R P WRP S
Sbjct: 52 KLRDRQQPPPHGVGRGEPGWRPAS 123
>TC224312
Length = 806
Score = 27.7 bits (60), Expect = 1.4
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 3/58 (5%)
Frame = -3
Query: 10 DPPTAANRRRPLWRPMSTQ-NAATGGDNSAKAVVAEQISQTVQSTSNLLH--LMQHSS 64
+ PTAAN P+W + N A G A A + ++ S+SNL L Q SS
Sbjct: 405 ETPTAANSTAPMWPTNAVSINEAIGSAAKANAAGRAMSTISIPSSSNLSQPLLKQRSS 232
>AW598757 similar to GP|2982431|emb leucine rich repeat-like protein
{Arabidopsis thaliana}, partial (6%)
Length = 415
Score = 27.3 bits (59), Expect = 1.8
Identities = 17/68 (25%), Positives = 34/68 (50%)
Frame = +2
Query: 34 GDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQ 93
G N +++ ++ IS ++ NL+HL S+ + NL + S + ++ Q+ Q
Sbjct: 176 GLNLSESSLSGSISTSLGRLQNLIHLDLSSNRLSGPIPPTLSNLTSLESLLLHSNQLTGQ 355
Query: 94 LPRVISSL 101
+P + SL
Sbjct: 356 IPTELHSL 379
>TC207380
Length = 627
Score = 27.3 bits (59), Expect = 1.8
Identities = 14/43 (32%), Positives = 27/43 (62%), Gaps = 5/43 (11%)
Frame = -1
Query: 25 MSTQNAATGGD-----NSAKAVVAEQISQTVQSTSNLLHLMQH 62
MS+Q ++GG +SA ++ E +++++S+S LH+ QH
Sbjct: 291 MSSQRVSSGGKFFTTLSSAGSLAKEV*TKSMESSSTALHICQH 163
>TC228128 similar to UP|Q9ZPL9 (Q9ZPL9) Nodule-enhanced protein phosphatase
type 2C, partial (51%)
Length = 807
Score = 26.9 bits (58), Expect = 2.4
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -2
Query: 11 PPTAANRRRPLWRPMS 26
PP A RR+PLW P S
Sbjct: 80 PPPAPRRRQPLWNPPS 33
>TC224754 similar to UP|Q94EU9 (Q94EU9) Beta-amylase PCT-BMYI , partial
(44%)
Length = 1108
Score = 26.6 bits (57), Expect = 3.1
Identities = 27/73 (36%), Positives = 31/73 (41%), Gaps = 10/73 (13%)
Frame = -2
Query: 46 ISQTVQSTSNLLHL----------MQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLP 95
ISQT QS SN L MQ L +L K L +S V + QQVL L
Sbjct: 690 ISQTPQSASNCLPQTTSYSFSDK*MQPIQNQSYLLTELEKMHLHHISQVHSPQQVLLLLF 511
Query: 96 RVISSLDAHMENG 108
+ SSLD G
Sbjct: 510 WLPSSLDEPTPQG 472
>TC232616 similar to GB|AAD25756.1|4587525|T5I8 Contains the PF|00650
CRAL/TRIO phosphatidyl-inositol-transfer protein domain.
ESTs gb|T76582, gb|N06574 and gb|Z25700 come from this
gene. {Arabidopsis thaliana;} , partial (48%)
Length = 1214
Score = 26.6 bits (57), Expect = 3.1
Identities = 15/53 (28%), Positives = 31/53 (58%), Gaps = 1/53 (1%)
Frame = -2
Query: 50 VQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVL-EQLPRVISSL 101
++ + LLH + S L+ PK+LL ++ T K T+ V+ ++P +I+++
Sbjct: 826 IKPLNTLLHQLAPPSQELTALLLSPKSLLIRLLTFKCTKDVVAHRVPLMINTI 668
>TC215180 homologue to UP|Q8LP17 (Q8LP17) Nine-cis-epoxycarotenoid
dioxygenase1, partial (26%)
Length = 708
Score = 26.2 bits (56), Expect = 4.1
Identities = 15/46 (32%), Positives = 25/46 (53%)
Frame = +2
Query: 70 LVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNVPQL 115
++K+P+NLL S K QQ+L Q + H+E + ++P L
Sbjct: 275 MMKIPENLLCMSSMQKQCQQILLQWS------NCHIEFHMVSMPSL 394
>TC211142 similar to GB|AAB68983.1|500656|YSCH9666 Yhr143wp {Saccharomyces
cerevisiae;} , partial (8%)
Length = 432
Score = 26.2 bits (56), Expect = 4.1
Identities = 17/68 (25%), Positives = 31/68 (45%)
Frame = +1
Query: 3 KMEQRRQDPPTAANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQH 62
K+ ++R T + R P+ + G+ K V E + +++ +++ LMQ
Sbjct: 223 KLREQRFKVETLSRRLSSELVPIRVHDVVINGNTKTKDWVIEAELKGIENATSMQELMQA 402
Query: 63 SSPAQAKL 70
S A AKL
Sbjct: 403 SQIAIAKL 426
>TC225899 similar to UP|O48685 (O48685) Pyruvate dehydrogenase E1 alpha
subunit , partial (40%)
Length = 700
Score = 25.8 bits (55), Expect = 5.3
Identities = 21/78 (26%), Positives = 33/78 (41%), Gaps = 10/78 (12%)
Frame = +1
Query: 7 RRQDPPTAANRRRPLWRP---MSTQNAAT----GGDNSAKAVVAEQISQTVQSTSNLLHL 59
R PPT A RR WRP S +++ T GG S + + +TS +
Sbjct: 271 RCPSPPTTATRRHAPWRPPRRSSLRSSTTWR*CGGWRSRRTRSTRRS*SAASATSTTVRR 450
Query: 60 MQHSS---PAQAKLVKLP 74
+ S+ P++A+ P
Sbjct: 451 LSPSAWRPPSRARTASSP 504
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.311 0.122 0.327
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,077,634
Number of Sequences: 63676
Number of extensions: 38609
Number of successful extensions: 287
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 286
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 287
length of query: 125
length of database: 12,639,632
effective HSP length: 86
effective length of query: 39
effective length of database: 7,163,496
effective search space: 279376344
effective search space used: 279376344
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144430.5