BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144375.7 - phase: 0
(123 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC214278 similar to UP|P93704 (P93704) HMG-1, partial (98%) 129 3e-31
TC214794 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein... 122 3e-29
TC214076 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein... 122 3e-29
TC224564 similar to PIR|T51159|T51159 HMG protein [imported] - A... 122 3e-29
TC214793 UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11 protei... 121 9e-29
TC224567 similar to PIR|T51159|T51159 HMG protein [imported] - A... 120 2e-28
BF068004 similar to SP|P26585|HMGL HMG1/2-like protein (SB11 pro... 99 5e-22
TC224760 similar to UP|O04692 (O04692) DNA-binding protein, part... 87 2e-21
TC208616 weakly similar to UP|Q41026 (Q41026) HMG 1 protein, par... 85 7e-18
BE023042 similar to SP|P26585|HMGL HMG1/2-like protein (SB11 pro... 83 3e-17
TC208617 weakly similar to UP|Q41026 (Q41026) HMG 1 protein, par... 82 5e-17
BU547954 72 6e-17
TC214450 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein... 81 1e-16
TC207913 weakly similar to UP|O49597 (O49597) HMG protein (HMG d... 74 1e-14
TC207912 weakly similar to UP|O49598 (O49598) HMG protein, parti... 73 3e-14
TC224722 similar to PIR|T51159|T51159 HMG protein [imported] - A... 69 4e-13
TC214451 weakly similar to UP|Q40094 (Q40094) High mobility grou... 67 2e-12
TC214412 homologue to UP|Q6V1W5 (Q6V1W5) Aminotransferase 1, par... 67 3e-12
TC214307 similar to UP|P93704 (P93704) HMG-1, partial (48%) 64 2e-11
BE059069 63 4e-11
>TC214278 similar to UP|P93704 (P93704) HMG-1, partial (98%)
Length = 932
Score = 129 bits (325), Expect = 3e-31
Identities = 69/122 (56%), Positives = 88/122 (71%), Gaps = 8/122 (6%)
Frame = +1
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK--- 60
+KA DP+KPKRP S FF+FMS+FR +F+KE+P+NK V+VVGKA GEKW+S+SDAEK
Sbjct: 370 RKAAKDPNKPKRPPSAFFVFMSEFREQFKKEHPNNKSVAVVGKAGGEKWKSLSDAEKAPF 549
Query: 61 -----KKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDDDDDDNEEEEEEDDEV 115
KKK EY K I AYNK++ GK S +S KS SE+NDD++D EEE+DDE
Sbjct: 550 VATAEKKKQEYEKTISAYNKQLEGKNSEEDES-DKSKSEVNDDEED------EEEDDDE* 708
Query: 116 RF 117
R+
Sbjct: 709 RW 714
>TC214794 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), complete
Length = 917
Score = 122 bits (307), Expect = 3e-29
Identities = 64/117 (54%), Positives = 81/117 (68%), Gaps = 9/117 (7%)
Frame = +3
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK---- 60
KA DP+KPKRP S FF+FM +FR F KE+PDNK VS VGKAAG KW++MSDAEK
Sbjct: 210 KAAKDPNKPKRPPSAFFVFMEEFRKVFNKEHPDNKAVSAVGKAAGAKWKTMSDAEKAPYV 389
Query: 61 ----KKKMEYVKAIHAYNKKVA-GKKSSSGKSFVKSNSELNDDDDDDDDNEEEEEED 112
K+K+EY K + AYNKK A G + KS SE+ND+DDD++ + EEE++D
Sbjct: 390 AKSEKRKVEYEKNMRAYNKKQAEGPTGGDEEESEKSVSEVNDEDDDEEGSGEEEDDD 560
>TC214076 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), complete
Length = 1064
Score = 122 bits (307), Expect = 3e-29
Identities = 64/117 (54%), Positives = 81/117 (68%), Gaps = 9/117 (7%)
Frame = +1
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK---- 60
KA DP+KPKRP S FF+FM +FR F KE+PDNK VS VGKAAG KW++MSDAEK
Sbjct: 361 KAAKDPNKPKRPPSAFFVFMEEFRKVFNKEHPDNKAVSAVGKAAGAKWKTMSDAEKAPYV 540
Query: 61 ----KKKMEYVKAIHAYNKKVA-GKKSSSGKSFVKSNSELNDDDDDDDDNEEEEEED 112
K+K+EY K + AYNKK A G + KS SE+ND+DDD++ + EEE++D
Sbjct: 541 AKSEKRKVEYEKNMRAYNKKQAEGPTGGDEEESEKSVSEVNDEDDDEEGSGEEEDDD 711
>TC224564 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (82%)
Length = 833
Score = 122 bits (307), Expect = 3e-29
Identities = 66/125 (52%), Positives = 85/125 (67%), Gaps = 14/125 (11%)
Frame = +1
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK--- 60
KKAK DP+KPKRP S FF+F+ +FR F+ ENP+ K VSVVGKA GEKW+S+S AEK
Sbjct: 214 KKAKKDPNKPKRPPSAFFVFLEEFRKTFKAENPNVKAVSVVGKAGGEKWKSLSSAEKAPY 393
Query: 61 -----KKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDD------DDDDNEEEE 109
K+K EY K I AY+KK A S+ + KS SE+ND+DD ++DD EEE+
Sbjct: 394 EAKAAKRKAEYEKLIKAYDKKQA--SSADDEESDKSKSEVNDEDDASGEEEEEDDEEEED 567
Query: 110 EEDDE 114
+EDD+
Sbjct: 568 DEDDD 582
>TC214793 UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11 protein), complete
Length = 1033
Score = 121 bits (303), Expect = 9e-29
Identities = 63/117 (53%), Positives = 81/117 (68%), Gaps = 9/117 (7%)
Frame = +3
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK---- 60
KA DP+KPKRP S FF+FM +FR F KE+P+NK VS VGKAAG KW++MSDAEK
Sbjct: 342 KAAKDPNKPKRPPSAFFVFMEEFRKVFNKEHPENKAVSAVGKAAGAKWKTMSDAEKAPYV 521
Query: 61 ----KKKMEYVKAIHAYNKKVA-GKKSSSGKSFVKSNSELNDDDDDDDDNEEEEEED 112
K+K+EY K + AYNKK A G + KS SE+ND+DDD++ + EEE++D
Sbjct: 522 AKSEKRKVEYEKNMRAYNKKQAEGPTGGDEEESEKSVSEVNDEDDDEEGSGEEEDDD 692
>TC224567 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (71%)
Length = 876
Score = 120 bits (300), Expect = 2e-28
Identities = 67/128 (52%), Positives = 82/128 (63%), Gaps = 17/128 (13%)
Frame = +2
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK--- 60
KKAK DP+KPKRP S FF+F+ +FR F+ ENP K VSVVGKA GEKW+S+S AEK
Sbjct: 257 KKAKKDPNKPKRPPSAFFVFLEEFRKTFKAENPLVKAVSVVGKAGGEKWKSLSSAEKAPY 436
Query: 61 -----KKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDD---------DDDDNE 106
K+K EY K I AY KK A S+ KS SE+ND+DD D+DD E
Sbjct: 437 EAKAAKRKAEYEKLIKAYEKKQA--SSADDDESDKSKSEVNDEDDASGEEDHQEDEDDEE 610
Query: 107 EEEEEDDE 114
EE++EDD+
Sbjct: 611 EEDDEDDD 634
>BF068004 similar to SP|P26585|HMGL HMG1/2-like protein (SB11 protein).
[Soybean] {Glycine max}, partial (98%)
Length = 448
Score = 99.0 bits (245), Expect = 5e-22
Identities = 54/115 (46%), Positives = 73/115 (62%), Gaps = 9/115 (7%)
Frame = +2
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK---- 60
KA DP+KP RP S F+ M +FR F K +P+NK +S VGKAAG KW++MSDAEK
Sbjct: 104 KAVKDPNKPNRPASAIFVLMEEFRNVFNK*HPENKALSAVGKAAGAKWKTMSDAEKAPYV 283
Query: 61 ----KKKMEYVKAIHAYNKKVAGKKSSSGKS-FVKSNSELNDDDDDDDDNEEEEE 110
K+K+EY K + AYNKK A + + KS S++ND+ DD+ + EEE+
Sbjct: 284 A*SEKRKVEYEKNMRAYNKKQAECPTGGDEE*SEKSVSDVNDEHDDEQSDGEEED 448
>TC224760 similar to UP|O04692 (O04692) DNA-binding protein, partial (73%)
Length = 654
Score = 87.4 bits (215), Expect(2) = 2e-21
Identities = 52/106 (49%), Positives = 64/106 (60%), Gaps = 17/106 (16%)
Frame = +3
Query: 26 DFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK--------KKKMEYVKAIHAYNKKV 77
+FR F+ ENP K VSVVGKA GEKW+S+S AEK K+K EY K I AY KK
Sbjct: 141 EFRKTFKAENPLVKAVSVVGKAGGEKWKSLSSAEKAPYEAKAAKRKAEYEKLIKAYEKKQ 320
Query: 78 AGKKSSSGKSFVKSNSELNDDDD---------DDDDNEEEEEEDDE 114
A S+ KS SE+ND+DD D+DD EEE++EDD+
Sbjct: 321 A--SSADDDESDKSKSEVNDEDDASGEEDHQEDEDDEEEEDDEDDD 452
Score = 30.4 bits (67), Expect(2) = 2e-21
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +1
Query: 7 KSDPSKPKRPQSPFFLFM 24
+ DP+KPKRP S FF+F+
Sbjct: 7 EKDPNKPKRPPSAFFVFL 60
>TC208616 weakly similar to UP|Q41026 (Q41026) HMG 1 protein, partial (51%)
Length = 880
Score = 85.1 bits (209), Expect = 7e-18
Identities = 48/116 (41%), Positives = 69/116 (59%), Gaps = 20/116 (17%)
Frame = +3
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEKKK- 62
KK K DP+ PKRP + FF+F+ DFR F++ NPD+K V VGK AGEKWRSM+D EKK
Sbjct: 186 KKVK-DPNMPKRPPTAFFVFLDDFRKSFKEANPDSKDVKRVGKEAGEKWRSMTDEEKKPY 362
Query: 63 -------KMEYVKAIHAY------------NKKVAGKKSSSGKSFVKSNSELNDDD 99
K EY KA+ +Y +K+ + K++++ + ++ EL D+D
Sbjct: 363 LDKVAELKEEYEKAMESYEAGQDEEDQTVSDKETSDKEAAAKEVAIEVEEELTDED 530
>BE023042 similar to SP|P26585|HMGL HMG1/2-like protein (SB11 protein).
[Soybean] {Glycine max}, partial (84%)
Length = 388
Score = 83.2 bits (204), Expect = 3e-17
Identities = 44/82 (53%), Positives = 55/82 (66%), Gaps = 8/82 (9%)
Frame = +2
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK---- 60
KA DPSKPKRP S F F+ +FR F KE+P+NK V VGK+A KW++MSDAEK
Sbjct: 104 KAAKDPSKPKRPASAVFDFIEEFRKVFSKEHPENKAVCAVGKSARTKWKTMSDAEKAPYV 283
Query: 61 ----KKKMEYVKAIHAYNKKVA 78
K+K+E+ K+I AY KK A
Sbjct: 284 AESEKRKVEFEKSIRAYIKKQA 349
>TC208617 weakly similar to UP|Q41026 (Q41026) HMG 1 protein, partial (51%)
Length = 872
Score = 82.4 bits (202), Expect = 5e-17
Identities = 47/111 (42%), Positives = 63/111 (56%)
Frame = +1
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEKKKK 63
KK K DP+ PKRP + FF+F+ DFR F++ NPD+K V VGK AGEKWRSM+D EKK
Sbjct: 295 KKVK-DPNMPKRPPTAFFVFLDDFRKSFKEANPDSKDVKRVGKEAGEKWRSMTDEEKK-- 465
Query: 64 MEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDDDDDDNEEEEEEDDE 114
Y KVA K+ K+ + +++D D E +E +E
Sbjct: 466 --------PYLDKVAELKAEYEKAMESYEAGQDEEDQAGSDKEAAAKEVEE 594
>BU547954
Length = 622
Score = 72.0 bits (175), Expect(2) = 6e-17
Identities = 39/78 (50%), Positives = 52/78 (66%), Gaps = 9/78 (11%)
Frame = -1
Query: 44 VGKAAGEKWRSMSDAEK--------KKKMEYVKAIHAYNKKVA-GKKSSSGKSFVKSNSE 94
VGKAAG KW++MSDAEK K+K+EY K + AYNKK A G + KS SE
Sbjct: 472 VGKAAGAKWKTMSDAEKAPYVAKSEKRKVEYEKNMRAYNKKQAEGPTGGDEEESEKSVSE 293
Query: 95 LNDDDDDDDDNEEEEEED 112
+ND+DDD++ + EEE++D
Sbjct: 292 VNDEDDDEEGSGEEEDDD 239
Score = 30.4 bits (67), Expect(2) = 6e-17
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -2
Query: 22 LFMSDFRVRFRKENPDNKYVSVV 44
+FM +FR F KE+P+NK VS V
Sbjct: 621 VFMEEFRKVFNKEHPENKAVSAV 553
>TC214450 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (72%)
Length = 548
Score = 80.9 bits (198), Expect = 1e-16
Identities = 36/56 (64%), Positives = 45/56 (80%)
Frame = +3
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK 60
KA DP+KPK+P + FF+FM +FR F KE+P+NK VS VGKAAG KW++MSDAEK
Sbjct: 327 KAAKDPNKPKKPPNAFFVFMEEFRKVFNKEHPENKAVSAVGKAAGAKWKTMSDAEK 494
>TC207913 weakly similar to UP|O49597 (O49597) HMG protein (HMG delta
protein), partial (41%)
Length = 517
Score = 74.3 bits (181), Expect = 1e-14
Identities = 39/112 (34%), Positives = 62/112 (54%), Gaps = 8/112 (7%)
Frame = +3
Query: 3 RKKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK-- 60
+ K K D KP++P + FF F+ DFR F+++NPD K + +GKA GEKW++M+ EK
Sbjct: 36 KNKQKFDAMKPRKPPTAFFYFLEDFRKEFQEQNPDVKSMRDIGKACGEKWKTMTYEEKVP 215
Query: 61 ------KKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDDDDDDNE 106
KK+ E+ A+ +NKK+ N E ++ DD+ + +E
Sbjct: 216 FYDIAPKKREEFDSAMAEFNKKM-------------ENGEFDETDDESEFDE 332
>TC207912 weakly similar to UP|O49598 (O49598) HMG protein, partial (35%)
Length = 758
Score = 73.2 bits (178), Expect = 3e-14
Identities = 39/112 (34%), Positives = 61/112 (53%), Gaps = 8/112 (7%)
Frame = +3
Query: 3 RKKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAGEKWRSMSDAEK-- 60
+ K D KPK+P + FF F+ DFR F+++NPD K + +GKA GEKW++M+ EK
Sbjct: 225 KNKQMFDAMKPKKPPTAFFYFLEDFRKEFQEQNPDVKSMRDIGKACGEKWKTMTYEEKVQ 404
Query: 61 ------KKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDDDDDDNE 106
KK+ E+ A+ +NKK+ N E ++ DD+ + +E
Sbjct: 405 YYDIATKKREEFDNAMAEFNKKM-------------ENGEFDETDDESEFDE 521
>TC224722 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (57%)
Length = 456
Score = 69.3 bits (168), Expect = 4e-13
Identities = 38/83 (45%), Positives = 46/83 (54%), Gaps = 26/83 (31%)
Frame = +2
Query: 4 KKAKSDPSKPKRPQSPFFLFM--------------------------SDFRVRFRKENPD 37
KKAK DP+KPKRP S FF+F+ +FR F+ ENP+
Sbjct: 170 KKAKKDPNKPKRPPSAFFVFLFVSSLLFPSYLSLRKMI*LIFLLCDSEEFRKTFKAENPN 349
Query: 38 NKYVSVVGKAAGEKWRSMSDAEK 60
K VSVVGKA GEKW+S+S AEK
Sbjct: 350 VKAVSVVGKAGGEKWKSLSSAEK 418
>TC214451 weakly similar to UP|Q40094 (Q40094) High mobility group protein 2
HMG2, partial (59%)
Length = 918
Score = 67.0 bits (162), Expect = 2e-12
Identities = 44/106 (41%), Positives = 60/106 (56%), Gaps = 8/106 (7%)
Frame = +2
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSV--------VGKAAGEKWRSM 55
KKA P+K KRP S FF+FMS+FR +F+KE+P+ +S + + +M
Sbjct: 197 KKAAKVPNKRKRPPSAFFIFMSEFREQFKKEHPNLLMISTFL*PSLH*FNVS*KSPFVAM 376
Query: 56 SDAEKKKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDDD 101
+ KKKK EY K I AYNKK+ GK +S K E+NDD+ D
Sbjct: 377 A---KKKKQEYKKTILAYNKKLEGKNPEEYES-DKLKFEVNDDEVD 502
>TC214412 homologue to UP|Q6V1W5 (Q6V1W5) Aminotransferase 1, partial (24%)
Length = 809
Score = 66.6 bits (161), Expect = 3e-12
Identities = 31/45 (68%), Positives = 35/45 (76%)
Frame = +3
Query: 5 KAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAAG 49
KA DP+KPKRP S FF+FM +FR F KE+PDNK VS VGKAAG
Sbjct: 672 KAAKDPNKPKRPPSAFFVFMEEFRKVFNKEHPDNKAVSAVGKAAG 806
>TC214307 similar to UP|P93704 (P93704) HMG-1, partial (48%)
Length = 553
Score = 63.9 bits (154), Expect = 2e-11
Identities = 39/77 (50%), Positives = 51/77 (65%), Gaps = 8/77 (10%)
Frame = +3
Query: 49 GEKWRSMSDAEK--------KKKMEYVKAIHAYNKKVAGKKSSSGKSFVKSNSELNDDDD 100
G+K S+SDAEK KKK EY K I AYNKK+ GK S +S KS SE+NDD++
Sbjct: 6 GDKS*SLSDAEKAPFVATAEKKKQEYEKTILAYNKKLEGKNSEEDES-DKSKSEVNDDEE 182
Query: 101 DDDDNEEEEEEDDEVRF 117
D+ EEE+++DDE R+
Sbjct: 183 DE---EEEDDDDDE*RW 224
>BE059069
Length = 411
Score = 62.8 bits (151), Expect = 4e-11
Identities = 29/45 (64%), Positives = 35/45 (77%)
Frame = +2
Query: 4 KKAKSDPSKPKRPQSPFFLFMSDFRVRFRKENPDNKYVSVVGKAA 48
KKAK DP+KPKRP S FF+F+ +FR F+ ENP+ K VSVVG AA
Sbjct: 71 KKAKKDPNKPKRPPSAFFVFLEEFRKTFKAENPNVKAVSVVGNAA 205
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.128 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,777,686
Number of Sequences: 63676
Number of extensions: 56125
Number of successful extensions: 3634
Number of sequences better than 10.0: 420
Number of HSP's better than 10.0 without gapping: 1169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2226
length of query: 123
length of database: 12,639,632
effective HSP length: 85
effective length of query: 38
effective length of database: 7,227,172
effective search space: 274632536
effective search space used: 274632536
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144375.7