BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144375.6 - phase: 0
(1419 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW394773 129 9e-30
BF009976 67 7e-11
TC211950 similar to UP|PNK1_ARATH (Q8L5Y9) Probable pantothenate... 46 1e-04
TC213405 similar to UP|Q96280 (Q96280) Orf12 protein, partial (8%) 43 0.001
BI498628 40 0.007
TC232402 homologue to UP|Q75I55 (Q75I55) Expressed protein, part... 38 0.028
BG654070 35 0.31
TC223062 similar to UP|Q96280 (Q96280) Orf12 protein, partial (8%) 34 0.40
TC222589 similar to UP|Q9LIR7 (Q9LIR7) Arabidopsis thaliana geno... 34 0.40
TC219088 similar to UP|Q8IP68 (Q8IP68) CG31813-PA, partial (9%) 34 0.40
TC205641 UP|Q8S5C1 (Q8S5C1) 4-coumarate:CoA ligase isoenzyme 2 ... 34 0.40
TC207392 similar to UP|BNK_DROME (P40794) Bottleneck protein, pa... 33 0.68
TC211378 similar to UP|Q6JCT1 (Q6JCT1) Cytochrome b (Fragment), ... 33 0.68
TC220240 similar to UP|TNF6_HUMAN (P48023) Tumor necrosis factor... 33 1.2
BM270199 32 1.5
TC209285 28 1.9
TC225961 similar to UP|Q8H9B8 (Q8H9B8) Low temperature and salt ... 32 2.0
TC216648 weakly similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, pa... 32 2.0
TC225959 similar to UP|Q8H9B8 (Q8H9B8) Low temperature and salt ... 32 2.0
BU549122 32 2.6
>AW394773
Length = 432
Score = 129 bits (324), Expect = 9e-30
Identities = 64/104 (61%), Positives = 80/104 (76%)
Frame = +1
Query: 561 NTNSTTKKLDLFRSQESKLTNLRKALSNQISPSKRKKGSESKSNKKVKVKAKSESSGSKQ 620
NTN+T K +D+F SQ+SKLTNLR + + ISP+KRK+ ++SK NKK KVKAKSE SGSKQ
Sbjct: 34 NTNNTGKNIDIFPSQDSKLTNLRHTVPSPISPAKRKRSTDSKQNKKAKVKAKSEPSGSKQ 213
Query: 621 ATITKFFGKAMPVMPGDTQSDQFGSKPGESPEVEELVPTDAGNM 664
ATIT+FF K +P MPG TQSD K +S +VE+L+PTD G M
Sbjct: 214 ATITRFFSKVIPEMPGGTQSDNSEPKLDQSSKVEDLLPTDDGQM 345
>BF009976
Length = 394
Score = 66.6 bits (161), Expect = 7e-11
Identities = 29/59 (49%), Positives = 37/59 (62%), Gaps = 1/59 (1%)
Frame = +1
Query: 44 PHSKLIPNTRFLIDSFRH-TTPSSFTYFLSHFHSDHYSPLSSSWSHGIIFCSPITSHLL 101
P K +P T F +D+FR+ YFL+HFH DHY LS WSHG I+CSP+T L+
Sbjct: 193 PFYKKMPGTMFTVDAFRYGCVEGCSAYFLTHFHCDHYGGLSKKWSHGPIYCSPLTGRLV 369
>TC211950 similar to UP|PNK1_ARATH (Q8L5Y9) Probable pantothenate kinase 1
(Pantothenic acid kinase 1) , partial (10%)
Length = 779
Score = 46.2 bits (108), Expect = 1e-04
Identities = 29/64 (45%), Positives = 36/64 (55%), Gaps = 8/64 (12%)
Frame = +2
Query: 1112 KNGCRIMSFQELSSRGRGGKDTLVTKESIKVGICIFVFD--------IMFANGEHLADPL 1163
KNGCRIMS LSS RG K T+VT E+IKV I + VF + + + L DPL
Sbjct: 83 KNGCRIMSL*VLSSSMRGRKHTIVTAETIKVAIGVLVFASDWWKWQLVRWQSHLELTDPL 262
Query: 1164 KYCL 1167
+ L
Sbjct: 263 CFSL 274
>TC213405 similar to UP|Q96280 (Q96280) Orf12 protein, partial (8%)
Length = 518
Score = 42.7 bits (99), Expect = 0.001
Identities = 19/38 (50%), Positives = 28/38 (73%), Gaps = 1/38 (2%)
Frame = +3
Query: 336 VPYSEHSNYEELREYVRFLKPKKVVPTVGLD-VEKSDS 372
VPYSEHS++ EL+E+VR + P ++P+V D E SD+
Sbjct: 69 VPYSEHSSFTELKEFVRVVSPDNIIPSVNNDGPESSDA 182
>BI498628
Length = 308
Score = 40.0 bits (92), Expect = 0.007
Identities = 30/83 (36%), Positives = 38/83 (45%), Gaps = 11/83 (13%)
Frame = +1
Query: 67 FTYFLSHFHSDHYSPLSSSWSHGIIFCSPITSHL-----------LINILHIPSPFVHPL 115
F L+H HSDH L+ S H +FCS +T+ L L ILH S H +
Sbjct: 61 FLQTLTHLHSDHTHGLTPS*HHAPLFCSAVTAKLLPFKFLGFDLSLFRILH--SGTTHTV 234
Query: 116 SLNQSVVIDGSVVTLIDANHCPG 138
L + VT+IDA HC G
Sbjct: 235 ILPSLTL----QVTVIDACHCLG 291
>TC232402 homologue to UP|Q75I55 (Q75I55) Expressed protein, partial (18%)
Length = 1098
Score = 38.1 bits (87), Expect = 0.028
Identities = 30/82 (36%), Positives = 39/82 (46%), Gaps = 4/82 (4%)
Frame = +2
Query: 58 SFRHTTPSSFTYFLSHFHS-DHYSPLSSSWSHGIIFCS---PITSHLLINILHIPSPFVH 113
S + TP T LS F + + Y+ L + HG++ S T H +I H PS F
Sbjct: 2 SHKLPTPPEMTLALSQFPALEVYAKLGGNARHGVVLSS*RQQQTLHQIIFSSHFPSSF-- 175
Query: 114 PLSLNQSVVIDGSVVTLIDANH 135
PL LNQS S+ L ANH
Sbjct: 176 PLVLNQSHPFPLSLSLLFKANH 241
>BG654070
Length = 391
Score = 34.7 bits (78), Expect = 0.31
Identities = 17/62 (27%), Positives = 33/62 (52%)
Frame = -3
Query: 471 VVQELSCCLPTWVTRSQMLDLISISGSNVVEAVSNFFERETEFHEQVNSSQTPVPTHRSC 530
+V ++SCC+ + +L+L+S + A + E FH+ +S Q P+P+ R+C
Sbjct: 188 IVSDVSCCI------NDLLELLSHDIRLTIRAALPY*SIEKAFHQTCHSVQQPLPSPRAC 27
Query: 531 SS 532
+
Sbjct: 26 KT 21
>TC223062 similar to UP|Q96280 (Q96280) Orf12 protein, partial (8%)
Length = 605
Score = 34.3 bits (77), Expect = 0.40
Identities = 14/41 (34%), Positives = 29/41 (70%)
Frame = +3
Query: 342 SNYEELREYVRFLKPKKVVPTVGLDVEKSDSKHVDKMRKYF 382
S++ EL+++V+ L+P K++PTV + ++ + +KM+ YF
Sbjct: 3 SSFSELQDFVQVLRPDKIIPTVNV----GNAANREKMQSYF 113
>TC222589 similar to UP|Q9LIR7 (Q9LIR7) Arabidopsis thaliana genomic DNA,
chromosome 3, BAC clone:F14O13, partial (16%)
Length = 1017
Score = 34.3 bits (77), Expect = 0.40
Identities = 34/134 (25%), Positives = 53/134 (39%), Gaps = 23/134 (17%)
Frame = +2
Query: 511 TEFHEQVNSSQTPVPTHRSCSSNDTSPL---------------------SKSNLKS--FS 547
TE E S++P P H S SP+ S+S L+S +S
Sbjct: 374 TEEKETKQKSRSPSPPHGRSRSKSRSPINYRRRRRSRSYSPARHSKDHRSRSPLRSHHYS 553
Query: 548 SNDASPFSKSNLNNTNSTTKKLDLFRSQESKLTNLRKALSNQISPSKRKKGSESKSNKKV 607
S D S ++ + ++ DL RS + + R+ S +SP RK SK +++
Sbjct: 554 SYDRERRSFRDIREHSDRYRRRDLDRSLDHHSSASRRNRSRSVSPYTRKSSXSSKRHRET 733
Query: 608 KVKAKSESSGSKQA 621
S G KQ+
Sbjct: 734 -----SPHRGRKQS 760
>TC219088 similar to UP|Q8IP68 (Q8IP68) CG31813-PA, partial (9%)
Length = 1012
Score = 34.3 bits (77), Expect = 0.40
Identities = 25/59 (42%), Positives = 29/59 (48%), Gaps = 5/59 (8%)
Frame = +3
Query: 2 APKSKSQTLESLNSTQLYLNALQSLNLPPPTLPLPP-----LPSSTIPHSKLIPNTRFL 55
APKS T+ + NS LYL + LPPPTLP P P IP+ PNT L
Sbjct: 273 APKSSLHTIGTSNS--LYLPSPLLQTLPPPTLPCNPTLTTMFPPHPIPNPN--PNTNIL 437
>TC205641 UP|Q8S5C1 (Q8S5C1) 4-coumarate:CoA ligase isoenzyme 2 , complete
Length = 1946
Score = 34.3 bits (77), Expect = 0.40
Identities = 41/131 (31%), Positives = 61/131 (46%), Gaps = 9/131 (6%)
Frame = +2
Query: 1 MAPKSKSQTLESLNSTQLYLNALQSLNLPPPTL---PLPPLPSSTIPHSKLIPNTRFLID 57
+AP S S+ L + T + +L+ PPP L P+PP+PSS+ P P T
Sbjct: 323 IAPNSSSRFLA--HPTAVPWPLPPTLSSPPPRLQSKPMPPMPSSSSPR----PLT----- 469
Query: 58 SFRHTTPSSFTYFLSHFHSDHYSPL----SSSWSHGIIFCSPITSHLLINILHIPSP--F 111
TT S + S + S P+ S S SH C IT L++ ++ I SP +
Sbjct: 470 ----TTKSRTSATSSSYSSTLVPPIQRRSSISISH---ICVRITVTLMLMLMLISSPMTW 628
Query: 112 VHPLSLNQSVV 122
+H L L + +V
Sbjct: 629 LHCLILREQLV 661
>TC207392 similar to UP|BNK_DROME (P40794) Bottleneck protein, partial (7%)
Length = 1544
Score = 33.5 bits (75), Expect = 0.68
Identities = 51/238 (21%), Positives = 85/238 (35%), Gaps = 22/238 (9%)
Frame = +2
Query: 492 ISISGSNVVEAVSNFFERETEFHEQVNSSQTPVPTHRSCSSNDTSPLSKSNLKSFSSNDA 551
+S GSN + F+ E E EQ P + N T L K S N
Sbjct: 131 LSSGGSNSRRRMLLEFDAEKEEFEQ--------PPKTKSTKNQTK-LIKPTTNLSSKNQT 283
Query: 552 SPFSKSNLNNTNSTTKKLDLFRSQESKLTNLRKALS----NQISPSKRKKGSESKSNKKV 607
+NLN++ + K + LTN + ++ N + +K S SK K
Sbjct: 284 KTIKSNNLNSSKNQLKLAKTKAAAGDTLTNTTEVVAVKKLNSTTLKSKKLNSTSKGLTKS 463
Query: 608 KVKAKSESSGSKQAT-----------ITKFFGKAMPVMPGDTQSDQFGSKPGESPE---- 652
+++SG K T +T K + D +S+ +K + +
Sbjct: 464 SSLDLAKTSGGKNKTTKATATNKDKEVTNKVTKFLDQTESDKKSNNTNNKKKKEQQGQPS 643
Query: 653 --VEELVPTDAGNMYKQEIDQFMQIINGD-ESLKKQAITIIEEAKGDINKALDIYYSN 707
E+ V D + +K +F Q + D E + + I +A +I + Y N
Sbjct: 644 WVFEDEVDDDLVSEFKDLPMKFQQTLIPDLERISTTSKAYITKANKEITRGFKPYVGN 817
>TC211378 similar to UP|Q6JCT1 (Q6JCT1) Cytochrome b (Fragment), partial (6%)
Length = 538
Score = 33.5 bits (75), Expect = 0.68
Identities = 19/66 (28%), Positives = 36/66 (53%)
Frame = +3
Query: 531 SSNDTSPLSKSNLKSFSSNDASPFSKSNLNNTNSTTKKLDLFRSQESKLTNLRKALSNQI 590
++N L + LKS SS+ +SPF +T + + LF + + +L ++LS ++
Sbjct: 276 NNNSMHSLIEMELKSSSSSSSSPF-----QTLINTIRSISLFTPKSPSVLSLPRSLSRKL 440
Query: 591 SPSKRK 596
S S+R+
Sbjct: 441 SSSRRR 458
>TC220240 similar to UP|TNF6_HUMAN (P48023) Tumor necrosis factor ligand
superfamily member 6 (FAS antigen ligand) (Apoptosis
antigen ligand) (APTL) (CD178 antigen), partial (7%)
Length = 837
Score = 32.7 bits (73), Expect = 1.2
Identities = 30/92 (32%), Positives = 39/92 (41%), Gaps = 2/92 (2%)
Frame = +2
Query: 29 PPPTLP-LPPLPSSTIPHSKLIPNTRFLIDSFRHTTPSSFTYFLSHFHSDHYSPLSSSWS 87
PPP LP LPP P PH FLI R P F++ + H P SS
Sbjct: 371 PPPRLPHLPPPPPPFFPH--------FLI---RRRFPLPFSFLIHQIH-----PYYSSRE 502
Query: 88 HGII-FCSPITSHLLINILHIPSPFVHPLSLN 118
H I+ F +P + L + P + P SL+
Sbjct: 503 HRILFFAAPPRAPQLHALAPTPQIHLRPQSLH 598
>BM270199
Length = 360
Score = 32.3 bits (72), Expect = 1.5
Identities = 18/55 (32%), Positives = 25/55 (44%)
Frame = +1
Query: 594 KRKKGSESKSNKKVKVKAKSESSGSKQATITKFFGKAMPVMPGDTQSDQFGSKPG 648
K+KK + K KK K K K ++ G T + G +P +PG KPG
Sbjct: 22 KKKKKKKKKKKKKKKKKKKKKNGGGGPGTQSPQKGVKLPPLPGRFLKTPGKEKPG 186
>TC209285
Length = 979
Score = 28.1 bits (61), Expect(2) = 1.9
Identities = 20/77 (25%), Positives = 35/77 (44%)
Frame = -2
Query: 91 IFCSPITSHLLINILHIPSPFVHPLSLNQSVVIDGSVVTLIDANHCPGAVQFLFKVNETE 150
IFC+ +TS + N S F HP +L +V+ V +I ++VN+ +
Sbjct: 846 IFCTLLTSIITHNSCSWSSSFSHPYTLLS*IVL----VWIIS-----------WRVNQLK 712
Query: 151 SPRYVHTGDFRFNREML 167
Y H ++N++ L
Sbjct: 711 GDVYTHLNQLKWNKQRL 661
Score = 22.3 bits (46), Expect(2) = 1.9
Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 5/37 (13%)
Frame = -1
Query: 50 PNTRFLIDSFRHT-----TPSSFTYFLSHFHSDHYSP 81
P + FL SF T T SSF HF HY+P
Sbjct: 964 PKSLFLPSSFSST*LTKTTTSSFIVLSPHF---HYTP 863
>TC225961 similar to UP|Q8H9B8 (Q8H9B8) Low temperature and salt responsive
protein, partial (98%)
Length = 607
Score = 32.0 bits (71), Expect = 2.0
Identities = 14/42 (33%), Positives = 28/42 (66%), Gaps = 1/42 (2%)
Frame = +2
Query: 77 DHYSPLSSSWSHGIIFCSP-ITSHLLINILHIPSPFVHPLSL 117
+ +S +++ WS G ++C P + +L +++L + SP HPL+L
Sbjct: 170 ESFSNMAARWSSGFVWC*PFLVIYLELSMLSMLSPSDHPLTL 295
>TC216648 weakly similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (41%)
Length = 2064
Score = 32.0 bits (71), Expect = 2.0
Identities = 71/339 (20%), Positives = 127/339 (36%), Gaps = 12/339 (3%)
Frame = +1
Query: 299 VERGYSKVVGFVPTGWTYEVKRDKFKVREK----------DSCKIHLVPYSE-HSNYEEL 347
++R + G+V WT + + K++EK D C + + ++ + EEL
Sbjct: 688 LKRNIGQFSGYV---WTENEDKQRAKIKEKIDKFVKEKLLDFCDVLNIQINKTNMKKEEL 858
Query: 348 R-EYVRFLKPKKVVPTVGLDVEKSDSKHVDKMRKYFAGLVDETANKHEFLKGFKQCDSGR 406
+ + FL+ P D+ +D + K R + KQ + +
Sbjct: 859 SAKLLEFLES----PHATTDILLADKEQKGKKRTRKSVPSKSPGEASTETPAKKQKQTSQ 1026
Query: 407 SGFEVGKDVGNDTEPGHSVEKEVKPSDVGGDKSIDQDVAMSLSSCMGETCIEDPTLLNDE 466
SG + K +D E + + + SD G D+DVA+ + +D ++E
Sbjct: 1027SG-KKQKQSSDDDE-----DDKAELSDAKGVSQEDEDVAVPNNES------DDEESRSEE 1170
Query: 467 EKEKVVQELSCCLPTWVTRSQMLDLISISGSNVVEAVSNFFERETEFHEQVNSSQTPVPT 526
E+EK T + + S+S ++ +V +TPV
Sbjct: 1171EEEKSKSRKR-------TSKKAVKESSVSKADRTSSVK----------------KTPVKD 1281
Query: 527 HRSCSSNDTSPLSKSNLKSFSSNDASPFSKSNLNNTNSTTKKLDLFRSQESKLTNLRKAL 586
+S P SK + S AS SKS ++ K+ Q++K K
Sbjct: 1282AKSIEKTKKKPTSKKGVAEHDSASAS-VSKSK---QPASKKQKTASEKQDTKGKAASKKR 1449
Query: 587 SNQISPSKRKKGSESKSNKKVKVKAKSESSGSKQATITK 625
+++ S + K +SKSNKK K + + + I K
Sbjct: 1450TDKSSKALVKDQGKSKSNKKAKAEPTKQDMHAVVVDILK 1566
>TC225959 similar to UP|Q8H9B8 (Q8H9B8) Low temperature and salt responsive
protein, partial (98%)
Length = 601
Score = 32.0 bits (71), Expect = 2.0
Identities = 14/42 (33%), Positives = 28/42 (66%), Gaps = 1/42 (2%)
Frame = +2
Query: 77 DHYSPLSSSWSHGIIFCSP-ITSHLLINILHIPSPFVHPLSL 117
+ +S +++ WS G ++C P + +L +++L + SP HPL+L
Sbjct: 182 ESFSNMAARWSSGFVWC*PFLVIYLELSMLSMLSPSDHPLTL 307
>BU549122
Length = 670
Score = 31.6 bits (70), Expect = 2.6
Identities = 24/58 (41%), Positives = 31/58 (53%)
Frame = +1
Query: 13 LNSTQLYLNALQSLNLPPPTLPLPPLPSSTIPHSKLIPNTRFLIDSFRHTTPSSFTYF 70
L Q L++LQ L P PLPP S TI HS++ P ++ + S TTPS F F
Sbjct: 247 LGPRQAPLHSLQPLT--PLLSPLPPNHSPTIFHSQIPPISQIPL-SKSPTTPSPFPLF 411
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.327 0.140 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,469,301
Number of Sequences: 63676
Number of extensions: 981413
Number of successful extensions: 8814
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 8341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8766
length of query: 1419
length of database: 12,639,632
effective HSP length: 109
effective length of query: 1310
effective length of database: 5,698,948
effective search space: 7465621880
effective search space used: 7465621880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC144375.6