BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.16 + phase: 0 /pseudo/partial
(221 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC224628 similar to UP|Q9ZST2 (Q9ZST2) Pyrophosphate-dependent p... 32 0.23
TC224758 similar to UP|PFPA_RICCO (Q41140) Pyrophosphate--fructo... 30 1.1
TC214804 29 1.5
TC230047 homologue to GB|AAN46796.1|24111345|BT001042 At1g34430/... 29 1.9
BM731889 28 2.5
TC210266 similar to UP|Q8LDN5 (Q8LDN5) Topoisomerase-like protei... 28 3.3
BG043254 28 3.3
TC220800 similar to UP|Q8H134 (Q8H134) At5g09860/MYH9_7, partial... 28 3.3
TC214023 similar to GB|BAA23769.1|2668492|D89981 metal-transport... 28 4.2
TC233180 similar to UP|Q944K0 (Q944K0) At1g18030/T10F20_3, parti... 27 5.5
TC229535 homologue to UP|Q8LK24 (Q8LK24) SOS2-like protein kinas... 27 5.5
BU926469 27 5.5
CA936032 27 5.5
TC226977 similar to UP|Q8S9L4 (Q8S9L4) At2g43020/MFL8.12, partia... 27 7.2
TC229250 similar to UP|Q9AR00 (Q9AR00) PSTVd RNA-biding protein,... 27 7.2
TC208881 UP|P93159 (P93159) Beta-glucan-elicitor receptor, complete 27 7.2
TC231062 weakly similar to UP|Q6N8I3 (Q6N8I3) CycJ cytochrome-c ... 27 9.5
TC232911 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B p... 27 9.5
BQ740358 27 9.5
TC205792 similar to UP|Q93X72 (Q93X72) Leucine-rich repeat resis... 27 9.5
>TC224628 similar to UP|Q9ZST2 (Q9ZST2) Pyrophosphate-dependent
phosphofructokinase alpha subunit , complete
Length = 2315
Score = 32.0 bits (71), Expect = 0.23
Identities = 21/64 (32%), Positives = 31/64 (47%), Gaps = 5/64 (7%)
Frame = -2
Query: 61 LRKPSGMELLCLRKLNLLQV-----RRRGMVMDTTRRKKLKWGWCQLEQVNQWLLLLLSM 115
L S + + L+ LN V R+ + TR KLKW W ++ +++ L LLS
Sbjct: 1879 LHNGSNLFKVLLKFLNAAHVVLVFNRKSYNLRTRTRTIKLKWTWISVDIIHKKALCLLSQ 1700
Query: 116 QLKC 119
QL C
Sbjct: 1699 QLIC 1688
>TC224758 similar to UP|PFPA_RICCO (Q41140) Pyrophosphate--fructose
6-phosphate 1-phosphotransferase alpha subunit (PFP)
(6-phosphofructokinase, pyrophosphate dependent)
(Pyrophosphate-dependent 6-phosphofructose-1-kinase)
(PPi-PFK) , partial (29%)
Length = 1043
Score = 29.6 bits (65), Expect = 1.1
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = -2
Query: 94 KLKWGWCQLEQVNQWLLLLLSMQLKC 119
KLKW W ++ +++ L LLS QL C
Sbjct: 382 KLKWTWISVDVIHKKALCLLSQQLIC 305
>TC214804
Length = 777
Score = 29.3 bits (64), Expect = 1.5
Identities = 18/54 (33%), Positives = 26/54 (47%)
Frame = -1
Query: 142 LCRRASLKCPSMRLPNR*NNRCRFNNNNKISKLGLLSLRYRCYMLSCFQLYSRE 195
L ++AS PSM LP+ +N C + NN + L C+ CF L S +
Sbjct: 429 LYQKASHPLPSMSLPSIQSNPCSYACNNSPASL--------CHFSPCFPLISSD 292
>TC230047 homologue to GB|AAN46796.1|24111345|BT001042 At1g34430/F7P12_2
{Arabidopsis thaliana;} , partial (21%)
Length = 858
Score = 28.9 bits (63), Expect = 1.9
Identities = 18/53 (33%), Positives = 29/53 (53%), Gaps = 5/53 (9%)
Frame = +3
Query: 52 RRWSLWEPVLRKPS--GMELLCLRKLNLLQVRRRGMVMDTTRR---KKLKWGW 99
RRW+L LR+PS ++L R + LL+ RG+++ RR + +W W
Sbjct: 678 RRWNLNRKRLRRPSRRRLQLRS*RPVILLRREGRGLLLHLMRRSSPRS*RWSW 836
>BM731889
Length = 422
Score = 28.5 bits (62), Expect = 2.5
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 16/72 (22%)
Frame = +2
Query: 75 LNLLQ-----VRRRGMVMDTTRRKKLKWGWCQLEQVNQWLL-----------LLLSMQLK 118
LNLL+ +R G V+D +K++K L VNQWL+ LL + K
Sbjct: 167 LNLLENLKTTLRVVGAVLDDAEKKQIK-----LSSVNQWLIELKDALYDADDLLDEISTK 331
Query: 119 CLRHTHICRILS 130
+C++LS
Sbjct: 332 SATQKKVCKVLS 367
>TC210266 similar to UP|Q8LDN5 (Q8LDN5) Topoisomerase-like protein, partial
(30%)
Length = 860
Score = 28.1 bits (61), Expect = 3.3
Identities = 14/39 (35%), Positives = 24/39 (60%), Gaps = 2/39 (5%)
Frame = -1
Query: 114 SMQLKCLRHTHICRILSIRSSHRFIISTLCRR--ASLKC 150
S + C+++ H C+I+S + H+ IS LCR S++C
Sbjct: 830 SKDVNCIQYVH-CKIISRFNKHKKPISNLCRTDDISIQC 717
>BG043254
Length = 421
Score = 28.1 bits (61), Expect = 3.3
Identities = 11/21 (52%), Positives = 16/21 (75%)
Frame = -2
Query: 136 RFIISTLCRRASLKCPSMRLP 156
RF+ S + RR SL CPS+++P
Sbjct: 354 RFLSSAMSRRKSLVCPSVQVP 292
>TC220800 similar to UP|Q8H134 (Q8H134) At5g09860/MYH9_7, partial (20%)
Length = 881
Score = 28.1 bits (61), Expect = 3.3
Identities = 25/75 (33%), Positives = 34/75 (45%)
Frame = +3
Query: 60 VLRKPSGMELLCLRKLNLLQVRRRGMVMDTTRRKKLKWGWCQLEQVNQWLLLLLSMQLKC 119
V RK + LRK+ LLQ++ R MVM LEQ+ Q L L +QL
Sbjct: 246 VKRKKQKALPIKLRKIRLLQMQLRSMVM-------------ALEQIPQQHLWNLMLQLPL 386
Query: 120 LRHTHICRILSIRSS 134
+ R+ + RSS
Sbjct: 387 VHKVGPQRLKNFRSS 431
>TC214023 similar to GB|BAA23769.1|2668492|D89981 metal-transporting P-type
ATPase {Arabidopsis thaliana;} , partial (21%)
Length = 828
Score = 27.7 bits (60), Expect = 4.2
Identities = 27/97 (27%), Positives = 44/97 (44%), Gaps = 14/97 (14%)
Frame = +3
Query: 66 GMELLCLRKLNLLQVRRRGMVMDTTRRKKLKWGWCQLEQVNQWLLLLLSMQLKCLRHTHI 125
GM L LR L RR + + R++ + ++ + QWL++ L+MQL L H +
Sbjct: 399 GMLLSMLRPLLEFPKRRCYLK*NQMRKRSS*MNYRKITTL*QWLVMELTMQLPWLLHMLV 578
Query: 126 ------------CRIL--SIRSSHRFIISTLCRRASL 148
C +L SSHR ++ TL ++L
Sbjct: 579 LH*VEVSGLLVRCLLLY*CATSSHRSLLFTLIFSSNL 689
>TC233180 similar to UP|Q944K0 (Q944K0) At1g18030/T10F20_3, partial (11%)
Length = 916
Score = 27.3 bits (59), Expect = 5.5
Identities = 12/21 (57%), Positives = 17/21 (80%), Gaps = 2/21 (9%)
Frame = +3
Query: 42 G*SSLPRTTFRR--WSLWEPV 60
G SS+P TTFRR W+++EP+
Sbjct: 291 GRSSMPSTTFRRRHWNVYEPL 353
>TC229535 homologue to UP|Q8LK24 (Q8LK24) SOS2-like protein kinase, partial
(34%)
Length = 969
Score = 27.3 bits (59), Expect = 5.5
Identities = 17/63 (26%), Positives = 28/63 (43%)
Frame = -2
Query: 47 PRTTFRRWSLWEPVLRKPSGMELLCLRKLNLLQVRRRGMVMDTTRRKKLKWGWCQLEQVN 106
P +T RW P+LR + + L + L+ + +V ++ L G+ QL
Sbjct: 194 PSSTCFRWQFLPPILRLAWPLNSVFLSRKPLISI--HSLVQPGPKQTTLPVGFSQLLNRT 21
Query: 107 QWL 109
QWL
Sbjct: 20 QWL 12
>BU926469
Length = 447
Score = 27.3 bits (59), Expect = 5.5
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = -3
Query: 92 RKKLKWGWCQLEQVNQWLLLLLSMQLKC 119
R++L G C+L Q++QW L L L C
Sbjct: 400 RRELGRG*CKLRQIHQWCLRWLIHSLHC 317
>CA936032
Length = 454
Score = 27.3 bits (59), Expect = 5.5
Identities = 32/126 (25%), Positives = 54/126 (42%), Gaps = 13/126 (10%)
Frame = -1
Query: 74 KLNLLQVRRRGMVMDTTRRKKLKWGWCQ-LEQVNQWLLLLLSMQLKCLRHTHIC------ 126
K +L +VR++ + TTR +++K + + Q N W +L K H H+
Sbjct: 418 KHHLRKVRKQVFIQRTTRVRQMKEKKKKTIYQYN*W*RTILG---KSCSHFHVLTSNAPL 248
Query: 127 -----RILSIRSSHRFIISTLCRRASLKCPSMRLPNR*NNRCRFNNNNKISKLGLLSL-R 180
+ +RSS + TLC R K + L CR N++ + + +L R
Sbjct: 247 SWQHKHLYFLRSSSSDSVDTLCIRLISKIQYLFLLEGGRRLCRRGRNHQRRRHRIRTLHR 68
Query: 181 YRCYML 186
RC +L
Sbjct: 67 NRCLLL 50
>TC226977 similar to UP|Q8S9L4 (Q8S9L4) At2g43020/MFL8.12, partial (14%)
Length = 629
Score = 26.9 bits (58), Expect = 7.2
Identities = 23/78 (29%), Positives = 36/78 (45%), Gaps = 4/78 (5%)
Frame = -2
Query: 104 QVNQWLLLLLSMQLKCLRHTHICR-ILSIRSSHRFII---STLCRRASLKCPSMRLPNR* 159
Q +QW+L SMQ +C + + ++S+ S+ R + LKCPS P
Sbjct: 625 QWSQWILWANSMQPRCTKVNRKAK*VISVNSATRSFP*F*KNNLK*GILKCPSSSNP--- 455
Query: 160 NNRCRFNNNNKISKLGLL 177
+ N+NN+ L LL
Sbjct: 454 -SHATSNDNNRWGPLLLL 404
>TC229250 similar to UP|Q9AR00 (Q9AR00) PSTVd RNA-biding protein, Virp1 (PSTVd
RNA-binding protein Virp1a) (PSTVd RNA-binding protein
Virp1b) (PSTVd RNA-binding protein Virp1c) (PSTVd
RNA-binding protein Virp1d), partial (34%)
Length = 1450
Score = 26.9 bits (58), Expect = 7.2
Identities = 14/54 (25%), Positives = 24/54 (43%), Gaps = 1/54 (1%)
Frame = -1
Query: 56 LWEPVLRKPSGMELLCLRKLNLLQVRR-RGMVMDTTRRKKLKWGWCQLEQVNQW 108
LW L+ + L + L+ V R ++ R+ WGWC +++QW
Sbjct: 1297 LWRQTLQP*PQLVFLFKTHIPLVGVLSLRLWLLQRLHRRSSHWGWCAHRRLHQW 1136
>TC208881 UP|P93159 (P93159) Beta-glucan-elicitor receptor, complete
Length = 2267
Score = 26.9 bits (58), Expect = 7.2
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 168 NNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLD 201
N+ + ++ + Y Y C + Y R+G+VQLD
Sbjct: 698 NSLVFRIRIRQQSYPDYARKCIRSYLRKGVVQLD 597
>TC231062 weakly similar to UP|Q6N8I3 (Q6N8I3) CycJ cytochrome-c biosynthesis
protein precursor, partial (41%)
Length = 837
Score = 26.6 bits (57), Expect = 9.5
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +3
Query: 45 SLPRTTFRRWSLWEPVLRKPSGMELLCLRKLNLLQVRRRGMV 86
+ PR R +++ P PS M +CLRKL LL R R ++
Sbjct: 411 TFPRRRGR*SAIFRPRRFSPSMMRSICLRKLLLLLRRIRRLL 536
>TC232911 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (34%)
Length = 843
Score = 26.6 bits (57), Expect = 9.5
Identities = 15/58 (25%), Positives = 25/58 (42%)
Frame = +1
Query: 116 QLKCLRHTHICRILSIRSSHRFIISTLCRRASLKCPSMRLPNR*NNRCRFNNNNKISK 173
+L CLRHT R H+F + +R++ C S + R R + + I +
Sbjct: 337 RLVCLRHTESQREAQQHREHKFRFENMAKRST--CKSRHMHGRIRRHQRHSEGSSIHR 504
>BQ740358
Length = 421
Score = 26.6 bits (57), Expect = 9.5
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = -2
Query: 109 LLLLLSMQLKCLRHTHICRILSIRSSHRFIISTLCRRAS 147
LL+LLS+ ++ H C+IL I S+ F LC R S
Sbjct: 381 LLVLLSITVRSPFQKHKCQILEI-STRSFDTQDLCSRVS 268
>TC205792 similar to UP|Q93X72 (Q93X72) Leucine-rich repeat resistance
protein-like protein, partial (93%)
Length = 1540
Score = 26.6 bits (57), Expect = 9.5
Identities = 15/29 (51%), Positives = 17/29 (57%)
Frame = -3
Query: 158 R*NNRCRFNNNNKISKLGLLSLRYRCYML 186
R* RF ++ IS L LL LRYRC L
Sbjct: 800 R*RRSGRFASSLGISPLKLLKLRYRCVKL 714
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.343 0.149 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,694,755
Number of Sequences: 63676
Number of extensions: 223114
Number of successful extensions: 2790
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 2775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2789
length of query: 221
length of database: 12,639,632
effective HSP length: 94
effective length of query: 127
effective length of database: 6,654,088
effective search space: 845069176
effective search space used: 845069176
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144345.16