BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.13 + phase: 0
(471 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222199 117 8e-27
TC216808 57 7e-18
BI317622 56 4e-08
BG508280 55 5e-08
BE611176 35 0.001
BI315644 33 0.27
TC216877 weakly similar to UP|Q753E9 (Q753E9) AFR365Cp, partial ... 29 4.0
TC218692 similar to UP|Q9SH45 (Q9SH45) F2K11.3, partial (47%) 29 5.2
TC221643 similar to UP|Q6P7C7 (Q6P7C7) Glycoprotein (Transmembra... 29 5.2
TC232048 similar to UP|ACOC_CUCMA (P49608) Aconitate hydratase, ... 28 8.8
>TC222199
Length = 691
Score = 117 bits (294), Expect = 8e-27
Identities = 66/226 (29%), Positives = 111/226 (48%), Gaps = 16/226 (7%)
Frame = +2
Query: 70 DYQLMPTLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKEKLTLKGNLPS 129
D+QL+PT+EE+ ++G P+ ++ P+ P + I + + + +
Sbjct: 5 DFQLVPTIEEFEEILGCPLGERKPYLSSGCLPSLSRIPTMVKDSARGLDRVKQTRNGIAG 184
Query: 130 LPTKFLYQQAFNFSKTNNVEAFYSILALLIYGLVLFPNIDNYVDIHAIQIFLT-----KN 184
LP K+L +A + + F +LALLI+G+VLFPN+D VD+ AI FL ++
Sbjct: 185 LPRKYLEDKARGIANQGDWVPFMDVLALLIFGVVLFPNVDGLVDLAAIDAFLAYHHSKES 364
Query: 185 PVPTLLADIYHSIHDRTQVGRGAILGCAPLLYKWFTSHLPQ----------THSFQANPE 234
PV +LAD++ + + R + I+ C P L W SHL Q +H
Sbjct: 365 PVVAVLADLFDTFNRRYEKSSARIICCLPALCVWLVSHLFQQDTRRPCPLLSHRSCTEKR 544
Query: 235 NLSWPKRIMSLTPSDITWY-RATCNFTNIIVSCGEYSNVPLLGTQG 279
+ W + + + I+W+ R ++ SCG Y N+PL+GT+G
Sbjct: 545 RIDWDQLLAGIGGRTISWFPRWKEGKEGVLFSCGRYPNIPLVGTRG 682
>TC216808
Length = 1144
Score = 57.0 bits (136), Expect(2) = 7e-18
Identities = 33/79 (41%), Positives = 46/79 (57%), Gaps = 5/79 (6%)
Frame = +3
Query: 150 AFYSILALLIYGLVLFPNIDNYVDIHAIQIFLT-----KNPVPTLLADIYHSIHDRTQVG 204
+F ILALLI+G+VLFPN++ VD+ AI FL ++PV +LA +Y + +
Sbjct: 591 SFIDILALLIFGVVLFPNMEGLVDLAAIDAFLAFHHGKESPVVAILA-MYDTFDRGCEKS 767
Query: 205 RGAILGCAPLLYKWFTSHL 223
I+ CA LY W SHL
Sbjct: 768 SARIVCCALALYVWLVSHL 824
Score = 51.6 bits (122), Expect(2) = 7e-18
Identities = 31/143 (21%), Positives = 63/143 (43%), Gaps = 2/143 (1%)
Frame = +2
Query: 12 RKLDLENLKKLAFEVTNLEN--FCDRHGKLLGVLWTNIEKGCLETLVQFYDPAYHCFTFP 69
+ L++ +L +L + L+ F +GK+ + + + +L Q+YD CFTF
Sbjct: 170 KSLEVTSLGELGQLMGQLQR*AFRKAYGKIWDLARVEVSMEPIASLSQYYDQPLRCFTFE 349
Query: 70 DYQLMPTLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKEKLTLKGNLPS 129
D+QL+PT++E + G + + P+ +P +A + + + + +
Sbjct: 350 DFQLVPTVKELEEIRGCQLGGRKPYLFSGFYPSTTRVAKVVKISAQELNRVKQNRNGVVG 529
Query: 130 LPTKFLYQQAFNFSKTNNVEAFY 152
+P K L ++A FY
Sbjct: 530 VPRKCLEERAKTLENQGEWAFFY 598
>BI317622
Length = 370
Score = 55.8 bits (133), Expect = 4e-08
Identities = 34/123 (27%), Positives = 57/123 (45%), Gaps = 16/123 (13%)
Frame = -2
Query: 169 DNYVDIHAIQIFLTKN-----PVPTLLADIYHSIHDRTQVGRGAILGCAPLLYKWFTSHL 223
D VD+ AI FL + P+ +LAD+Y + + R + I+ C P LY W SHL
Sbjct: 369 DGLVDLAAIGAFLAYHDYKEIPIVAMLADLYDTFNRRCEKSSTRIVCCTPALYVWLVSHL 190
Query: 224 ----------PQTHSFQANPENLSWPKRIMSLTPSDITWY-RATCNFTNIIVSCGEYSNV 272
++H +W + + + + + W+ R T +++SCG + NV
Sbjct: 189 FRQEVRHACPLESHCSCTEKGGENWGQLLANKEGASVNWFPRWKEGRTRVLISCGVFLNV 10
Query: 273 PLL 275
PL+
Sbjct: 9 PLM 1
>BG508280
Length = 418
Score = 55.5 bits (132), Expect = 5e-08
Identities = 32/96 (33%), Positives = 54/96 (55%), Gaps = 2/96 (2%)
Frame = +1
Query: 260 TNIIVSCGEYSNVPLLGTQGGISYNPILAKRQFGCPMEAKP--DNIYLQGEFYFNHEDPS 317
T +++SCG + NVPL+GT+G ISYNP+LA +Q G PM P D + FN +
Sbjct: 64 TRVLISCGGFPNVPLMGTRGCISYNPVLAIKQLGYPM*GVPLEDELAPVISRGFNKTNVE 243
Query: 318 NKRGRFVQAWHAIRTLNRSQLARRSDSLQGSYTQWV 353
+ + +AW ++ ++ +L ++ G Y +W+
Sbjct: 244 TLQ-KVRKAWEVLQKKDK-ELRGSNNGPIGGYRKWL 345
>BE611176
Length = 424
Score = 35.4 bits (80), Expect(2) = 0.001
Identities = 19/62 (30%), Positives = 32/62 (50%), Gaps = 2/62 (3%)
Frame = -2
Query: 14 LDLENLKKLA--FEVTNLENFCDRHGKLLGVLWTNIEKGCLETLVQFYDPAYHCFTFPDY 71
LD+ ++K+L ++ F + K+L + I + +L Q+YD CFTF D+
Sbjct: 330 LDITSIKELGRLMGPLQMQAFRKAYEKILDLTIAEISTEAIASLTQYYD*PLRCFTFGDF 151
Query: 72 QL 73
QL
Sbjct: 150 QL 145
Score = 24.6 bits (52), Expect(2) = 0.001
Identities = 10/29 (34%), Positives = 18/29 (61%), Gaps = 2/29 (6%)
Frame = -1
Query: 73 LMPTLEEYSHLIGLPVLDKVP--FTGLEP 99
+ PT+EE+ ++G P+ + P F+G P
Sbjct: 148 IRPTVEEFEEILGCPLRGRKPYFFSGFLP 62
>BI315644
Length = 421
Score = 33.1 bits (74), Expect = 0.27
Identities = 14/26 (53%), Positives = 18/26 (68%)
Frame = +3
Query: 277 TQGGISYNPILAKRQFGCPMEAKPDN 302
T+G I+YNP+LA RQ G PM P +
Sbjct: 276 TRGCINYNPVLAIRQLGYPMRGVPSD 353
>TC216877 weakly similar to UP|Q753E9 (Q753E9) AFR365Cp, partial (17%)
Length = 511
Score = 29.3 bits (64), Expect = 4.0
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -2
Query: 377 PALPLAPATVEECQERLAQSECVGATWKR 405
P P +PATV + +A C GA W R
Sbjct: 225 PTQPKSPATVSDDGSEVATPHCTGADWDR 139
>TC218692 similar to UP|Q9SH45 (Q9SH45) F2K11.3, partial (47%)
Length = 644
Score = 28.9 bits (63), Expect = 5.2
Identities = 26/62 (41%), Positives = 31/62 (49%), Gaps = 2/62 (3%)
Frame = -1
Query: 76 TLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKEKL--TLKGNLPSLPTK 133
T YS I L + DKVP GLE A L+ SL++E TL+ NLP L K
Sbjct: 404 TFLTYSSKISLLLFDKVPLGGLESPALAG-------LELSLLEESYASTLRPNLP-LGFK 249
Query: 134 FL 135
FL
Sbjct: 248 FL 243
>TC221643 similar to UP|Q6P7C7 (Q6P7C7) Glycoprotein (Transmembrane) nmb,
partial (3%)
Length = 558
Score = 28.9 bits (63), Expect = 5.2
Identities = 21/74 (28%), Positives = 34/74 (45%)
Frame = +1
Query: 367 PRYLSSTTPAPALPLAPATVEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEH 426
P YLSST+P+PA P+ E + R++ S + A K E+E+++
Sbjct: 55 PPYLSSTSPSPA--STPSITERSKPRISDSLNLNA-----------KTTNSDEEVEEKDE 195
Query: 427 EVHKLRRQIVKKNV 440
E + R +K V
Sbjct: 196 ETWETRETKREKKV 237
>TC232048 similar to UP|ACOC_CUCMA (P49608) Aconitate hydratase, cytoplasmic
(Citrate hydro-lyase) (Aconitase) , partial (28%)
Length = 882
Score = 28.1 bits (61), Expect = 8.8
Identities = 17/62 (27%), Positives = 27/62 (43%), Gaps = 3/62 (4%)
Frame = -2
Query: 252 WYRATCNFTNIIV---SCGEYSNVPLLGTQGGISYNPILAKRQFGCPMEAKPDNIYLQGE 308
W FT + S Y+ + + T G SYN I + CP+E + N+ L+
Sbjct: 701 WLHLQSQFTCFLCNQCSSKHYARI*SICTTGDSSYNNITVPKMCWCPLECELCNLILRFF 522
Query: 309 FY 310
+Y
Sbjct: 521 WY 516
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.136 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,991,995
Number of Sequences: 63676
Number of extensions: 363176
Number of successful extensions: 1966
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1961
length of query: 471
length of database: 12,639,632
effective HSP length: 101
effective length of query: 370
effective length of database: 6,208,356
effective search space: 2297091720
effective search space used: 2297091720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144345.13