BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143341.7 + phase: 0
(745 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222041 similar to UP|Q8LBQ6 (Q8LBQ6) Isp4-like protein, partia... 367 e-101
BQ080748 weakly similar to PIR|T08925|T08 isp4 protein homolog T... 270 2e-72
BM085219 weakly similar to GP|9758213|dbj sexual differentiation... 258 5e-69
TC222979 similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partia... 241 1e-63
CO978558 218 1e-56
AW310775 weakly similar to PIR|T08925|T08 isp4 protein homolog T... 194 2e-49
AI736639 188 7e-48
TC213712 similar to UP|Q9LWW1 (Q9LWW1) ESTs D25093(R3139) (Gluta... 104 1e-22
BG839504 similar to GP|20466754|gb Isp4-like protein {Arabidopsi... 104 2e-22
TC223528 weakly similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein,... 70 4e-12
AI959812 67 4e-11
CK605780 65 1e-10
TC206603 similar to UP|Q9LSD4 (Q9LSD4) Gb|AAF23830.1, partial (47%) 34 0.21
TC224701 homologue to PRF|1909359A.0|445612|1909359A ribosomal p... 34 0.21
TC206604 similar to UP|Q9LSD4 (Q9LSD4) Gb|AAF23830.1, partial (37%) 33 0.35
TC228384 33 0.60
TC221285 similar to UP|Q6R3L0 (Q6R3L0) Metal-nicotianamine trans... 33 0.60
CA853857 similar to PIR|H96681|H96 protein F1E22.10 [imported] -... 32 0.79
TC226304 similar to GB|AAN72000.1|25082773|BT001989 expressed pr... 32 0.79
TC224807 homologue to PRF|1909359A.0|445612|1909359A ribosomal p... 32 0.79
>TC222041 similar to UP|Q8LBQ6 (Q8LBQ6) Isp4-like protein, partial (38%)
Length = 1012
Score = 367 bits (941), Expect = e-101
Identities = 162/284 (57%), Positives = 209/284 (73%), Gaps = 2/284 (0%)
Frame = +2
Query: 462 IALIFTLPIGVIEATTNIRSGLNVITELVIGFIYPGKPLANVAFKTYGHISMVQALGFLG 521
+A FTLPI +I ATTN GLN+ITE V G IYPG+P+ANV FKTYG+ISM QA+ FL
Sbjct: 2 LAFGFTLPISIITATTNQTPGLNIITEYVFGLIYPGRPIANVCFKTYGYISMAQAVSFLS 181
Query: 522 DFKLGHYMKIAPKSMFIVQLVGTVVASSVHFGTAWWLLTSIENICDESLLPKGSPWTCPG 581
DFKLGHYMKI P+SMF+VQ +GT++A +++ G AWWLL SI+NIC + LLP+GSPWTCPG
Sbjct: 182 DFKLGHYMKIPPRSMFLVQFIGTILAGTINIGVAWWLLNSIKNICHDDLLPEGSPWTCPG 361
Query: 582 DDVFYNASIIWGVVGPKRMFTKDGVYPELNWFFLIGLIAPVPVWLLSLKFPNQKWIQFIN 641
D VF++AS+IWG+VGPKR+F G Y +NWFFL G + P+ VWLL FP Q WI IN
Sbjct: 362 DRVFFDASVIWGLVGPKRIFGSQGNYSAMNWFFLGGALGPIIVWLLHKAFPKQSWIPLIN 541
Query: 642 IPIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAFIG 701
+P+++ +PP +NY W VG FNF+++R K WW R+ Y+LSA LD+GVAF+
Sbjct: 542 LPVLLGATGMMPPATPLNYNAWIFVGTIFNFFIFRYRKKWWQRYNYVLSAALDSGVAFMT 721
Query: 702 LLLYFSLQSYGIYGPT--WWGLEPDHCPLAKCPTAPGVHAEGCP 743
++LYFSL G+ + WWG + +HCPLA CPTA G+ +GCP
Sbjct: 722 VMLYFSL---GLENKSLNWWGNDGEHCPLAACPTAKGIIVDGCP 844
>BQ080748 weakly similar to PIR|T08925|T08 isp4 protein homolog T15N24.40 -
Arabidopsis thaliana, partial (17%)
Length = 421
Score = 270 bits (689), Expect = 2e-72
Identities = 122/139 (87%), Positives = 130/139 (92%)
Frame = +3
Query: 576 PWTCPGDDVFYNASIIWGVVGPKRMFTKDGVYPELNWFFLIGLIAPVPVWLLSLKFPNQK 635
PWTCPGDDVFYNASIIWGVVGPKRMFTKDGVYP +NWFFLIGL+AP+PVWLLS KFPN K
Sbjct: 3 PWTCPGDDVFYNASIIWGVVGPKRMFTKDGVYPGMNWFFLIGLLAPLPVWLLSKKFPNHK 182
Query: 636 WIQFINIPIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDA 695
WIQ IN PIIIAGAS+IPP RSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSA LDA
Sbjct: 183 WIQLINFPIIIAGASNIPPFRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAALDA 362
Query: 696 GVAFIGLLLYFSLQSYGIY 714
GVAF+G+ LYF+LQS GI+
Sbjct: 363 GVAFMGVALYFALQSNGIF 419
>BM085219 weakly similar to GP|9758213|dbj sexual differentiation process
protein ISP4-like {Arabidopsis thaliana}, partial (17%)
Length = 426
Score = 258 bits (660), Expect = 5e-69
Identities = 111/139 (79%), Positives = 127/139 (90%)
Frame = -2
Query: 582 DDVFYNASIIWGVVGPKRMFTKDGVYPELNWFFLIGLIAPVPVWLLSLKFPNQKWIQFIN 641
D+VFYNASIIWGVVGP+RMFTKDG+YP +NWFFLIGL+APVPVWLL+ KFPN KWI+ IN
Sbjct: 425 DEVFYNASIIWGVVGPQRMFTKDGIYPGMNWFFLIGLLAPVPVWLLARKFPNHKWIELIN 246
Query: 642 IPIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAFIG 701
+P+IIAG IPP RSVNYITWG VGIFFNFYVY KFKAWWARHTYILSA LDAGVAF+G
Sbjct: 245 MPLIIAGGGGIPPARSVNYITWGFVGIFFNFYVYSKFKAWWARHTYILSAALDAGVAFMG 66
Query: 702 LLLYFSLQSYGIYGPTWWG 720
++LYF+LQ+YG++GP WWG
Sbjct: 65 VILYFALQNYGVFGPIWWG 9
>TC222979 similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partial (30%)
Length = 661
Score = 241 bits (614), Expect = 1e-63
Identities = 108/216 (50%), Positives = 146/216 (67%)
Frame = +2
Query: 497 GKPLANVAFKTYGHISMVQALGFLGDFKLGHYMKIAPKSMFIVQLVGTVVASSVHFGTAW 556
GKP+AN+ FK YG IS V AL FL D KLGHYMKI P+ M+ QL GT+ A + AW
Sbjct: 2 GKPIANLLFKIYGRISTVHALSFLSDLKLGHYMKIPPRCMYTAQLEGTLFAGVENLAVAW 181
Query: 557 WLLTSIENICDESLLPKGSPWTCPGDDVFYNASIIWGVVGPKRMFTKDGVYPELNWFFLI 616
W+ SI++IC + L SPWTCP V ++AS+IWG++GPKR+F G+Y L W FLI
Sbjct: 182 WM*DSIKDICMDDKLHHDSPWTCPKYRVTFDASVIWGLIGPKRLFGPGGLYRNLVWLFLI 361
Query: 617 GLIAPVPVWLLSLKFPNQKWIQFINIPIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYR 676
G PVP+W+LS FP +KWI INIP+I + +PP N +W + G+ FN++V+R
Sbjct: 362 GAELPVPIWVLSKIFPEKKWIPLINIPVITYRFAGMPPATPANIASWLVTGMIFNYFVFR 541
Query: 677 KFKAWWARHTYILSAGLDAGVAFIGLLLYFSLQSYG 712
K WW ++ Y+LSA LDA AF+G+L++F+LQ+ G
Sbjct: 542 YNKRWWQKYNYVLSAALDAXTAFMGVLIFFALQNAG 649
>CO978558
Length = 721
Score = 218 bits (554), Expect = 1e-56
Identities = 94/205 (45%), Positives = 138/205 (66%)
Frame = -2
Query: 540 QLVGTVVASSVHFGTAWWLLTSIENICDESLLPKGSPWTCPGDDVFYNASIIWGVVGPKR 599
Q+VGT+++ ++ TAWWL+ +I ++CD ++L SPWTCP D+VF++AS+IWG++GP+R
Sbjct: 717 QVVGTILSVFIYTVTAWWLMRTIPHLCDTTMLDPDSPWTCPMDNVFFDASVIWGLLGPRR 538
Query: 600 MFTKDGVYPELNWFFLIGLIAPVPVWLLSLKFPNQKWIQFINIPIIIAGASDIPPVRSVN 659
+F G Y ++N FFL G IAP VWL FP Q+WI I++P+++ S +PP +VN
Sbjct: 537 IFGDLGEYAKVNLFFLGGAIAPFLVWLAHKAFPEQRWITLIHMPVLLGATSMMPPATAVN 358
Query: 660 YITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAFIGLLLYFSLQSYGIYGPTWW 719
+ +W +VG + YR + WW R Y+LS GLDAG AF+ +LL+ +L + I WW
Sbjct: 357 FTSWILVGFLSGYIAYRYRQEWWKRCNYVLSGGLDAGTAFMTILLFLALNNNNIM-LNWW 181
Query: 720 GLEPDHCPLAKCPTAPGVHAEGCPV 744
G P+ CPLA CPT G+ +GCPV
Sbjct: 180 GNNPEGCPLANCPTEKGIVVKGCPV 106
>AW310775 weakly similar to PIR|T08925|T08 isp4 protein homolog T15N24.40 -
Arabidopsis thaliana, partial (14%)
Length = 466
Score = 194 bits (492), Expect = 2e-49
Identities = 84/113 (74%), Positives = 97/113 (85%), Gaps = 1/113 (0%)
Frame = -1
Query: 633 NQKWIQFINIPIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAG 692
N KWI+ IN+P+IIAG+ IPP RSVNYITWG+VGI FNFYVY KFKAWWARH YILSA
Sbjct: 466 NHKWIELINMPLIIAGSGGIPPARSVNYITWGMVGILFNFYVYNKFKAWWARHNYILSAA 287
Query: 693 LDAGVAFIGLLLYFSLQSYGIYGPTWWGLE-PDHCPLAKCPTAPGVHAEGCPV 744
LDAGVAF+G++LYF+LQ Y I+GPTWWG + DHCPLAKCPTAPG+ + GCPV
Sbjct: 286 LDAGVAFMGVILYFALQYYDIFGPTWWGQDADDHCPLAKCPTAPGIISHGCPV 128
>AI736639
Length = 427
Score = 188 bits (478), Expect = 7e-48
Identities = 81/141 (57%), Positives = 109/141 (76%)
Frame = +2
Query: 171 GWAGIFRRFLVDSPYMWWPESLVQVSLFRAFHEKEKRPRGGTSRLQFFFVVFVASFAYYI 230
GWAG+FR+FLV+ MWWP +LVQVSLF A HEK KRP+GGT+R QFF +V V+ AYY+
Sbjct: 5 GWAGLFRKFLVEPGEMWWPSNLVQVSLFSALHEKSKRPKGGTTRTQFFLLVLVSGLAYYV 184
Query: 231 IPGYFFQAISTVSFVCLIWKESITAQQIGSGMKGLGIGSFGLDWNTVAGFLGSPLAVPGF 290
PGY F +++ S++C + +S+ QQ+GSG++GLGI +FG+DW+T++ +LGSPLA P F
Sbjct: 185 FPGYLFSMLTSFSWMCWLAPKSVLVQQLGSGLRGLGIAAFGIDWSTISAYLGSPLASPWF 364
Query: 291 AIINIMAGFFLYMYVLIPISY 311
A NI GFFL MYV+ PI+Y
Sbjct: 365 ATANIAVGFFLVMYVMTPIAY 427
>TC213712 similar to UP|Q9LWW1 (Q9LWW1) ESTs D25093(R3139) (Glutathione
transporter), partial (14%)
Length = 393
Score = 104 bits (260), Expect = 1e-22
Identities = 49/105 (46%), Positives = 62/105 (58%)
Frame = +2
Query: 640 INIPIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAF 699
IN+P++I +PP +VNY +W I G F V+R WW RH Y+LS LDAG+AF
Sbjct: 14 INMPVLIGATGMMPPATAVNYTSWIIAGFLSGFVVFRYKPEWWKRHNYVLSGALDAGLAF 193
Query: 700 IGLLLYFSLQSYGIYGPTWWGLEPDHCPLAKCPTAPGVHAEGCPV 744
+G+LLY L I WWG + D C LA CPTA V +GC V
Sbjct: 194 MGVLLYLCLGLEDI-SLNWWGNDLDGCTLAHCPTAKVVEVQGCAV 325
>BG839504 similar to GP|20466754|gb Isp4-like protein {Arabidopsis thaliana},
partial (15%)
Length = 499
Score = 104 bits (259), Expect = 2e-22
Identities = 46/94 (48%), Positives = 62/94 (65%), Gaps = 2/94 (2%)
Frame = +3
Query: 652 IPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAFIGLLLYFSLQSY 711
+PP +NY W VG FNF+++R WW R+ Y+LSA LD+GVAF+ +L YF+L
Sbjct: 75 MPPATPLNYNAWIFVGTIFNFFIFRYRXKWWXRYNYVLSAALDSGVAFMTVLXYFAL--- 245
Query: 712 GIYGPT--WWGLEPDHCPLAKCPTAPGVHAEGCP 743
G+ + WWG + +HCPLA CPTA GV +GCP
Sbjct: 246 GLENKSXNWWGNDGEHCPLAVCPTAKGVIVDGCP 347
>TC223528 weakly similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partial
(4%)
Length = 399
Score = 69.7 bits (169), Expect = 4e-12
Identities = 26/33 (78%), Positives = 30/33 (90%)
Frame = +3
Query: 712 GIYGPTWWGLEPDHCPLAKCPTAPGVHAEGCPV 744
GI+GPTWWGL+ DHC LAKCPTAP VHA+GCP+
Sbjct: 6 GIFGPTWWGLDADHCHLAKCPTAPDVHAKGCPL 104
>AI959812
Length = 193
Score = 66.6 bits (161), Expect = 4e-11
Identities = 26/59 (44%), Positives = 40/59 (67%)
Frame = -2
Query: 409 DVHTRIMKKNYEQVPEWWFVTILILMVMMALLACEGFGKQLQLPWWGILLSLTIALIFT 467
D+HTR+M++ Y+ VP WWF IL + + + CE + + LQLPWWG+LL+ I++ T
Sbjct: 174 DIHTRLMRR-YKSVPTWWFYIILAANIALIIFICEYYNESLQLPWWGVLLACAISIFRT 1
>CK605780
Length = 491
Score = 65.1 bits (157), Expect = 1e-10
Identities = 29/78 (37%), Positives = 39/78 (49%)
Frame = -3
Query: 643 PIIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAFIGL 702
P I A IPP W IVG+ FN+++ R + WW R+ Y+LS LD G A +
Sbjct: 471 PSIFGAAGQIPPATCWYLGQWVIVGLIFNYFIRRAYFGWWTRYNYVLSGALDIGTALCIV 292
Query: 703 LLYFSLQSYGIYGPTWWG 720
+ +L G P WWG
Sbjct: 291 VSALALGLSGASFPDWWG 238
>TC206603 similar to UP|Q9LSD4 (Q9LSD4) Gb|AAF23830.1, partial (47%)
Length = 1062
Score = 34.3 bits (77), Expect = 0.21
Identities = 34/141 (24%), Positives = 55/141 (38%)
Frame = +1
Query: 423 PEWWFVTILILMVMMALLACEGFGKQLQLPWWGILLSLTIALIFTLPIGVIEATTNIRSG 482
P W++ + ++ AL C +G L W + A+T + G
Sbjct: 502 PLKWYLVLCSYILAPALAFCNSYGTGLTD--WSL------------------ASTYGKIG 621
Query: 483 LNVITELVIGFIYPGKPLANVAFKTYGHISMVQALGFLGDFKLGHYMKIAPKSMFIVQLV 542
L +I V G +A VA + A + DFK G+ + KSMF+ QL+
Sbjct: 622 LFIIAAAVG---QNGGVIAGVAACAVMMSIVATAADLMQDFKTGYLTLSSAKSMFVSQLI 792
Query: 543 GTVVASSVHFGTAWWLLTSIE 563
GT + + T W T+ +
Sbjct: 793 GTAMGCVIAPLTFWMFWTAFD 855
>TC224701 homologue to PRF|1909359A.0|445612|1909359A ribosomal protein S19.
{Solanum tuberosum;} , complete
Length = 849
Score = 34.3 bits (77), Expect = 0.21
Identities = 22/86 (25%), Positives = 42/86 (48%)
Frame = -1
Query: 203 EKEKRPRGGTSRLQFFFVVFVASFAYYIIPGYFFQAISTVSFVCLIWKESITAQQIGSGM 262
+K + R G + FFF F AS A + F A+ +SF+C + ++ + +
Sbjct: 576 QKNLKIRTGCLKTHFFFPAFAASDALVFLTPRIFLALFFLSFICFLDFSTLVSSPF---L 406
Query: 263 KGLGIGSFGLDWNTVAGFLGSPLAVP 288
L +GS+ L ++T + +P+ +P
Sbjct: 405 ISLYLGSYFLAFSTES*IKPNPVDLP 328
>TC206604 similar to UP|Q9LSD4 (Q9LSD4) Gb|AAF23830.1, partial (37%)
Length = 1091
Score = 33.5 bits (75), Expect = 0.35
Identities = 20/67 (29%), Positives = 31/67 (45%)
Frame = +2
Query: 497 GKPLANVAFKTYGHISMVQALGFLGDFKLGHYMKIAPKSMFIVQLVGTVVASSVHFGTAW 556
G +A VA + A + DFK G+ + KSMF+ QL+GT + + T W
Sbjct: 143 GGVIAGVASCAVMMSIVATAADLMQDFKTGYLTLSSAKSMFVSQLIGTAMGCVIAPLTFW 322
Query: 557 WLLTSIE 563
T+ +
Sbjct: 323 MFWTAFD 343
>TC228384
Length = 1332
Score = 32.7 bits (73), Expect = 0.60
Identities = 20/42 (47%), Positives = 23/42 (54%), Gaps = 7/42 (16%)
Frame = -3
Query: 80 SAQIIALPLGKLM-------AATLPTKPIQVPFTTWSFSLNP 114
SA ++ LP GKL A TLPT+PI FTT SL P
Sbjct: 820 SAPLLLLPSGKLTT*GLG*WAETLPTEPISCEFTTGKQSLPP 695
>TC221285 similar to UP|Q6R3L0 (Q6R3L0) Metal-nicotianamine transporter YSL1,
partial (32%)
Length = 945
Score = 32.7 bits (73), Expect = 0.60
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +1
Query: 522 DFKLGHYMKIAPKSMFIVQLVGTVV 546
DFK +Y +PK+MFI QLVGT +
Sbjct: 103 DFKTAYYTCTSPKAMFICQLVGTAL 177
>CA853857 similar to PIR|H96681|H96 protein F1E22.10 [imported] - Arabidopsis
thaliana, partial (24%)
Length = 531
Score = 32.3 bits (72), Expect = 0.79
Identities = 23/75 (30%), Positives = 38/75 (50%)
Frame = -2
Query: 522 DFKLGHYMKIAPKSMFIVQLVGTVVASSVHFGTAWWLLTSIENICDESLLPKGSPWTCPG 581
DFK G+ +P+SMF+ Q++GT + + W + N+ +P GS + P
Sbjct: 326 DFKTGYMTLASPRSMFLSQVLGTAMGCVISPCVFWLFYKAFGNLG----IP-GSAYPAPY 162
Query: 582 DDVFYNASIIWGVVG 596
V+ N +I+ GV G
Sbjct: 161 ALVYRNMAIL-GVDG 120
>TC226304 similar to GB|AAN72000.1|25082773|BT001989 expressed protein
{Arabidopsis thaliana;} , partial (87%)
Length = 1345
Score = 32.3 bits (72), Expect = 0.79
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +3
Query: 345 ETFDIDMESYSNYSKIYLSVAFAFEYGFCFAALTATISHVVLFH 388
ETF D E++ ++IY+S+ + FC+ L T V LFH
Sbjct: 582 ETFMADHEAWRELAEIYVSLQMYKQAAFCYEELILTQPTVPLFH 713
>TC224807 homologue to PRF|1909359A.0|445612|1909359A ribosomal protein S19.
{Solanum tuberosum;} , complete
Length = 955
Score = 32.3 bits (72), Expect = 0.79
Identities = 20/86 (23%), Positives = 42/86 (48%)
Frame = -1
Query: 203 EKEKRPRGGTSRLQFFFVVFVASFAYYIIPGYFFQAISTVSFVCLIWKESITAQQIGSGM 262
+K + + G + FFF AS A+ + F A+ +SF+C + ++ + +
Sbjct: 592 QKNLKIKTGCLKTHFFFPALAASEAFVFLTPRIFLALFFLSFICFLDFSTLVSSPF---L 422
Query: 263 KGLGIGSFGLDWNTVAGFLGSPLAVP 288
L +GS+ L ++T + +P+ +P
Sbjct: 421 ISLYLGSYFLAFSTES*IKPNPVDLP 344
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.141 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,815,454
Number of Sequences: 63676
Number of extensions: 600243
Number of successful extensions: 4356
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 4271
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4343
length of query: 745
length of database: 12,639,632
effective HSP length: 104
effective length of query: 641
effective length of database: 6,017,328
effective search space: 3857107248
effective search space used: 3857107248
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC143341.7