BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143341.10 + phase: 0
(633 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232083 weakly similar to GB|AAH66215.1|42490896|BC066215 Ighmb... 339 3e-93
TC230009 similar to UP|SMB2_HUMAN (P38935) DNA-binding protein S... 231 6e-61
TC230008 weakly similar to UP|O27671 (O27671) Transcriptional co... 147 1e-58
TC230007 weakly similar to GB|AAA58611.1|183250|HUMGLIA1F glial ... 181 9e-46
TC230778 similar to GB|AAK40099.1|13811296|AF339908 RNA helicase... 83 3e-16
BF424356 homologue to GP|24059907|db putaitive UPF1 {Oryza sativ... 73 3e-13
TC209483 similar to UP|RNT1_HUMAN (Q92900) Regulator of nonsense... 72 6e-13
TC229971 weakly similar to GB|CAB10335.1|2244913|ATFCA4 SEN1 lik... 70 4e-12
BM085282 similar to GP|13811296|g RNA helicase SDE3 {Arabidopsis... 57 2e-08
TC224185 weakly similar to UP|Q9SHX6 (Q9SHX6) F1E22.16, partial ... 54 2e-07
AI960553 weakly similar to PIR|B96682|B96 protein F1E22.14 [impo... 44 3e-04
TC219704 43 5e-04
TC204690 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type A... 33 0.50
TC205434 homologue to UP|PRS6_ARATH (Q9SEI4) 26S protease regula... 32 0.86
AI443839 32 1.1
TC204689 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type A... 32 1.1
BF070568 homologue to GP|10798790|dbj replication factor C 36kDa... 31 1.5
TC230028 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 ... 31 1.9
TC204913 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, ... 31 1.9
TC230029 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 ... 31 1.9
>TC232083 weakly similar to GB|AAH66215.1|42490896|BC066215 Ighmbp2 protein
{Mus musculus;} , partial (14%)
Length = 566
Score = 339 bits (869), Expect = 3e-93
Identities = 167/188 (88%), Positives = 185/188 (97%)
Frame = +2
Query: 255 RIGHPARLLPQVVDSALDAQVLRGDNSGLANDIRKEMKVLNGKLLKTKEKNTRREIQKEL 314
R+GHPARLLPQV+DSALDAQVLRGDNSGLANDIRKEMK LNGKLLKTK++NTR++IQ+EL
Sbjct: 2 RLGHPARLLPQVLDSALDAQVLRGDNSGLANDIRKEMKALNGKLLKTKDRNTRKDIQREL 181
Query: 315 RTLSREERKRQQLAVTDVIKTSDVILTTLIGASSKKLGNTSFDLVIIDEAAQALEVACWI 374
RTLS+EERKRQQLAVTDV+K++DVILTTLIGA SKKL +TSFDLVIIDEAAQALE+ACWI
Sbjct: 182 RTLSKEERKRQQLAVTDVLKSADVILTTLIGAFSKKLDSTSFDLVIIDEAAQALEIACWI 361
Query: 375 PLLKGTRCILAGDHLQLPPTIQSVEAEKKGLGRTLFERLAELYGDEVTSMLTVQYRMHQL 434
P+LKG+RCILAGDHLQLPPTIQSVEAEKKGLGRTLFERLAE+YGDE+TSMLTVQYRMH+L
Sbjct: 362 PILKGSRCILAGDHLQLPPTIQSVEAEKKGLGRTLFERLAEVYGDEITSMLTVQYRMHEL 541
Query: 435 IMDWSSKE 442
IMDWSSKE
Sbjct: 542 IMDWSSKE 565
>TC230009 similar to UP|SMB2_HUMAN (P38935) DNA-binding protein SMUBP-2
(Immunoglobulin MU binding protein 2) (SMUBP-2) (Glial
factor-1) (GF-1), partial (5%)
Length = 719
Score = 231 bits (590), Expect = 6e-61
Identities = 117/137 (85%), Positives = 123/137 (89%)
Frame = +1
Query: 497 NEGESEVAMAHAKRLVQSGVLPSDIGIITPYAAQVVLLKMLKNKENSLKDIEISTVDGFQ 556
NEGE+E + HAKR VQSGV+ D IITPYAAQVVLLKMLKNKE LKD+EISTVDGFQ
Sbjct: 1 NEGEAEXTVXHAKRXVQSGVIXXDXXIITPYAAQVVLLKMLKNKEXRLKDVEISTVDGFQ 180
Query: 557 GREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTRARRQCCIVCDTETVSSDGFLKRLIE 616
GREKEAIIISMVRSNSKKEVGFLSD RRMNVAVTR+RRQCC+V DTETVS DGFLKRLIE
Sbjct: 181 GREKEAIIISMVRSNSKKEVGFLSDHRRMNVAVTRSRRQCCLVSDTETVSGDGFLKRLIE 360
Query: 617 YFEEHGEYQSASEYQNE 633
YFEEHGEY SASEYQNE
Sbjct: 361 YFEEHGEYLSASEYQNE 411
>TC230008 weakly similar to UP|O27671 (O27671) Transcriptional control factor
(Enhancer-binding protein), partial (10%)
Length = 700
Score = 147 bits (371), Expect(2) = 1e-58
Identities = 77/98 (78%), Positives = 82/98 (83%)
Frame = +3
Query: 536 MLKNKENSLKDIEISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTRARRQ 595
MLKNKE+ LKD+EISTVDGFQGREKEAIIISMVRSNS VGFLSD RRMN VTR+ RQ
Sbjct: 342 MLKNKEDQLKDVEISTVDGFQGREKEAIIISMVRSNSFFLVGFLSDHRRMNGGVTRSTRQ 521
Query: 596 CCIVCDTETVSSDGFLKRLIEYFEEHGEYQSASEYQNE 633
CC+V DTET S DGFLKR IE FEEHGE SASE Q+E
Sbjct: 522 CCLVSDTETASGDGFLKR*IESFEEHGESLSASECQSE 635
Score = 98.6 bits (244), Expect(2) = 1e-58
Identities = 49/60 (81%), Positives = 53/60 (87%)
Frame = +2
Query: 475 LLLIDTAGCDMEEKKDEEDSTLNEGESEVAMAHAKRLVQSGVLPSDIGIITPYAAQVVLL 534
LL++ CDMEEKKDEEDST NEGE+EV + HAKRLVQSGVLPSDIGIITPYAAQVVLL
Sbjct: 158 LLILMRFRCDMEEKKDEEDSTFNEGEAEVTVTHAKRLVQSGVLPSDIGIITPYAAQVVLL 337
>TC230007 weakly similar to GB|AAA58611.1|183250|HUMGLIA1F glial factor-1
{Homo sapiens;} , partial (22%)
Length = 491
Score = 181 bits (459), Expect = 9e-46
Identities = 88/96 (91%), Positives = 93/96 (96%)
Frame = +3
Query: 538 KNKENSLKDIEISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTRARRQCC 597
KNKE+ LKD+EISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTR+RRQCC
Sbjct: 9 KNKEDQLKDVEISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTRSRRQCC 188
Query: 598 IVCDTETVSSDGFLKRLIEYFEEHGEYQSASEYQNE 633
+VCDTETVS DGFLKRLIEYFE+HGEY SASEYQNE
Sbjct: 189 LVCDTETVSGDGFLKRLIEYFEDHGEYLSASEYQNE 296
>TC230778 similar to GB|AAK40099.1|13811296|AF339908 RNA helicase SDE3
{Arabidopsis thaliana;} , partial (17%)
Length = 989
Score = 83.2 bits (204), Expect = 3e-16
Identities = 56/156 (35%), Positives = 85/156 (53%), Gaps = 9/156 (5%)
Frame = +1
Query: 482 GCDMEEKKDEEDSTLNEGESEVAMAHAKRLVQSG-VLPSDIGIITPYAAQVVLLKMLKNK 540
GCD E++ S N E+ + +RL+ G + +IGIITPY QV LK+ +
Sbjct: 100 GCD--EREGNNPSWFNRIEASKVIEVVRRLIAGGNIKEENIGIITPYRQQV--LKIKQTL 267
Query: 541 EN-SLKDIEISTVDGFQGREKEAIIISMVRSNSKKE-------VGFLSDRRRMNVAVTRA 592
EN + +I++ +V+ FQG+EKE IIIS VRS K +GFLS+ RR NVA+TRA
Sbjct: 268 ENLDMPEIKVGSVEQFQGQEKEVIIISTVRSTIKHNEFDRVHCLGFLSNYRRFNVAITRA 447
Query: 593 RRQCCIVCDTETVSSDGFLKRLIEYFEEHGEYQSAS 628
I+ + + D +++ + ++ YQ S
Sbjct: 448 ISLLVIIGNPHIICKDDHWSQMLWHCVDNSSYQGCS 555
>BF424356 homologue to GP|24059907|db putaitive UPF1 {Oryza sativa (japonica
cultivar-group)}, partial (7%)
Length = 298
Score = 73.2 bits (178), Expect = 3e-13
Identities = 35/75 (46%), Positives = 54/75 (71%)
Frame = +2
Query: 547 IEISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTRARRQCCIVCDTETVS 606
IE+++VD FQGREK+ II+S VRSN + +GFL+D RR+NVA+TRAR I+ + + +S
Sbjct: 2 IEVASVDSFQGREKDYIILSCVRSNEHQGIGFLNDPRRLNVALTRARYGIVILGNPKVLS 181
Query: 607 SDGFLKRLIEYFEEH 621
+ L+ +++EH
Sbjct: 182 NQPLWNSLLTHYKEH 226
>TC209483 similar to UP|RNT1_HUMAN (Q92900) Regulator of nonsense transcripts
1 (Nonsense mRNA reducing factor 1) (NORF1)
(Up-frameshift suppressor 1 homolog), partial (3%)
Length = 811
Score = 72.4 bits (176), Expect = 6e-13
Identities = 45/94 (47%), Positives = 66/94 (69%), Gaps = 6/94 (6%)
Frame = +3
Query: 507 HAKRLVQS-GVLPSDIGIITPYAAQVVLLK-----MLKNKENSLKDIEISTVDGFQGREK 560
H ++ V+S G+ +GIITPY Q+ L+ +L ++E KD+ I+TVD FQG+E+
Sbjct: 93 HVQKTVKSLGLGKITVGIITPYKLQLKCLQREFDEVLNSEEG--KDLYINTVDAFQGQER 266
Query: 561 EAIIISMVRSNSKKEVGFLSDRRRMNVAVTRARR 594
+ II+S VR++S VGF++D RRMNVA+TRARR
Sbjct: 267 DVIIMSCVRASS-HGVGFVADIRRMNVALTRARR 365
>TC229971 weakly similar to GB|CAB10335.1|2244913|ATFCA4 SEN1 like protein
{Arabidopsis thaliana;} , partial (14%)
Length = 919
Score = 69.7 bits (169), Expect = 4e-12
Identities = 39/105 (37%), Positives = 60/105 (57%), Gaps = 3/105 (2%)
Frame = +1
Query: 519 SDIGIITPYAAQVVLLKMLKNKENSL---KDIEISTVDGFQGREKEAIIISMVRSNSKKE 575
+ + II+PY+ QV L + + + K ++I TVDG QGREK+ I S VR++ K
Sbjct: 100 NQVAIISPYSQQVKLFQKRFEETFGMSAEKVVDICTVDGCQGREKDIAIFSCVRASKDKG 279
Query: 576 VGFLSDRRRMNVAVTRARRQCCIVCDTETVSSDGFLKRLIEYFEE 620
+GF+ D RRMNV +TRA+ +V T+ +L+E E+
Sbjct: 280 IGFVEDIRRMNVGITRAKSAVLVVGSASTLRRSEQWNKLVESAEK 414
>BM085282 similar to GP|13811296|g RNA helicase SDE3 {Arabidopsis thaliana},
partial (13%)
Length = 424
Score = 57.0 bits (136), Expect = 2e-08
Identities = 32/73 (43%), Positives = 45/73 (60%), Gaps = 4/73 (5%)
Frame = +3
Query: 179 INKNLDYSQKDAISKALSSKNV--FLLHGPPGTGKTTTVVEIILQEVK--RGSKILACAA 234
I+ + Q +I L K +++HGPPGTGKT T+VE ILQ K + ++IL CA
Sbjct: 27 ISGTFNEEQISSIKMILGCKGAPPYMIHGPPGTGKTRTMVEAILQLYKYHKNARILVCAP 206
Query: 235 SNIAVDNIVERLV 247
SN A D I+E+L+
Sbjct: 207 SNSAADYILEKLL 245
>TC224185 weakly similar to UP|Q9SHX6 (Q9SHX6) F1E22.16, partial (9%)
Length = 592
Score = 54.3 bits (129), Expect = 2e-07
Identities = 28/73 (38%), Positives = 45/73 (61%), Gaps = 6/73 (8%)
Frame = -1
Query: 180 NKNLDYSQKDAISKALSSKN------VFLLHGPPGTGKTTTVVEIILQEVKRGSKILACA 233
++NL+ SQK+A+ ++S+ + L+ GPPGTGKT TV ++ +K ++ LACA
Sbjct: 565 SQNLNQSQKEAVVSCVTSRECHHNDTIKLIWGPPGTGKTKTVASLLFSLLKLKARTLACA 386
Query: 234 ASNIAVDNIVERL 246
+N AV + RL
Sbjct: 385 PTNTAVLEVAARL 347
>AI960553 weakly similar to PIR|B96682|B96 protein F1E22.14 [imported] -
Arabidopsis thaliana, partial (5%)
Length = 279
Score = 43.5 bits (101), Expect = 3e-04
Identities = 20/44 (45%), Positives = 28/44 (63%)
Frame = -2
Query: 202 LLHGPPGTGKTTTVVEIILQEVKRGSKILACAASNIAVDNIVER 245
L+ GPPGTGKT TV ++ +K ++ LACA +N AV + R
Sbjct: 164 LIWGPPGTGKTKTVASLLFSLLKLKARTLACAPTNTAVLEVAAR 33
>TC219704
Length = 889
Score = 42.7 bits (99), Expect = 5e-04
Identities = 22/53 (41%), Positives = 37/53 (69%)
Frame = +3
Query: 548 EISTVDGFQGREKEAIIISMVRSNSKKEVGFLSDRRRMNVAVTRARRQCCIVC 600
+++TVD FQG++ + I++S+VR+ + VG L D RR+ VA++RAR + C
Sbjct: 9 QVTTVDKFQGQQNDFILLSIVRT---RFVGHLRDVRRLVVAMSRARLGLYVFC 158
>TC204690 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATPase,
partial (94%)
Length = 1630
Score = 32.7 bits (73), Expect = 0.50
Identities = 39/166 (23%), Positives = 72/166 (42%), Gaps = 9/166 (5%)
Frame = +2
Query: 201 FLLHGPPGTGKTTTVVEIILQEVKRGSKILACAASNIAVD--NIVERLVPHRVKLVRIGH 258
FLL+GPPGTGK+ + + S + ++S++ E+LV + ++ R
Sbjct: 632 FLLYGPPGTGKSYLAKAV---ATEADSTFFSVSSSDLVSKWMGESEKLVSNLFQMARESA 802
Query: 259 PARLLPQVVDSALDAQVLRGDNSGLANDIRKEMKVL-------NGKLLKTKEKNTRREIQ 311
P+ + +DS L Q G+ S + I+ E+ V + K+L NT +
Sbjct: 803 PSIIFVDEIDS-LCGQRGEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALD 979
Query: 312 KELRTLSREERKRQQLAVTDVIKTSDVILTTLIGASSKKLGNTSFD 357
+ +R R KR + + D +K + +G + L + F+
Sbjct: 980 QAIR---RRFDKRIYIPLPD-LKARQHMFKVHLGDTPHNLAESDFE 1105
>TC205434 homologue to UP|PRS6_ARATH (Q9SEI4) 26S protease regulatory subunit
6B homolog (26S proteasome AAA-ATPase subunit RPT3)
(Regulatory particle triple-A ATPase subunit 3), partial
(96%)
Length = 1573
Score = 32.0 bits (71), Expect = 0.86
Identities = 28/98 (28%), Positives = 42/98 (42%), Gaps = 11/98 (11%)
Frame = +1
Query: 126 LEKVANEVTYHRMKDALIQLSKGVHKGPASDLIPVLFGERQPTVSKKDVVFTSINKNLDY 185
L K + V HR +AL+ V A I +L +P V+ D+ I K
Sbjct: 451 LLKPSASVALHRHSNALVD----VLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQ--- 609
Query: 186 SQKDAISKALSSKNVF-----------LLHGPPGTGKT 212
++A+ L+ ++ LL+GPPGTGKT
Sbjct: 610 EIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKT 723
>AI443839
Length = 360
Score = 31.6 bits (70), Expect = 1.1
Identities = 14/30 (46%), Positives = 19/30 (62%)
Frame = +3
Query: 200 VFLLHGPPGTGKTTTVVEIILQEVKRGSKI 229
+ LLHGP G GKTT + + +L E +G I
Sbjct: 66 LLLLHGPRGVGKTTLINQRLLPEWNKGPHI 155
>TC204689 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATPase,
partial (98%)
Length = 1726
Score = 31.6 bits (70), Expect = 1.1
Identities = 40/166 (24%), Positives = 75/166 (45%), Gaps = 9/166 (5%)
Frame = +3
Query: 201 FLLHGPPGTGKTTTVVEIILQEVKRGSKILACAASNIAVD--NIVERLVPHRVKLVRIGH 258
FLL+GPPGTGK + + + + E + S + ++S++ E+LV + ++ R
Sbjct: 681 FLLYGPPGTGK-SYLAKAVATEAE--STFFSVSSSDLVSKWMGESEKLVSNLFEMARESA 851
Query: 259 PARLLPQVVDSALDAQVLRGDNSGLANDIRKEMKVL-------NGKLLKTKEKNTRREIQ 311
P+ + +DS L Q G+ S + I+ E+ V + K+L NT +
Sbjct: 852 PSIIFIDEIDS-LCGQRGEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALD 1028
Query: 312 KELRTLSREERKRQQLAVTDVIKTSDVILTTLIGASSKKLGNTSFD 357
+ +R R KR + + D +K + +G + L + F+
Sbjct: 1029QAIR---RRFDKRIYIPLPD-LKARQHMFKVHLGDTPHNLTESDFE 1154
>BF070568 homologue to GP|10798790|dbj replication factor C 36kDa subunit
{Oryza sativa (japonica cultivar-group)}, partial (15%)
Length = 348
Score = 31.2 bits (69), Expect = 1.5
Identities = 11/17 (64%), Positives = 16/17 (93%)
Frame = +3
Query: 202 LLHGPPGTGKTTTVVEI 218
LL+GPPGTGKT+T++ +
Sbjct: 294 LLYGPPGTGKTSTILAV 344
>TC230028 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 {Arabidopsis
thaliana;} , partial (24%)
Length = 435
Score = 30.8 bits (68), Expect = 1.9
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +1
Query: 202 LLHGPPGTGKTTTVVEIILQ 221
L +GPPGTGKTTT + I Q
Sbjct: 331 LFYGPPGTGKTTTALAIAHQ 390
>TC204913 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, partial
(14%)
Length = 1080
Score = 30.8 bits (68), Expect = 1.9
Identities = 31/109 (28%), Positives = 53/109 (48%), Gaps = 6/109 (5%)
Frame = +3
Query: 202 LLHGPPGTGKTTTVVEIILQEVKRGSKILACAASNIAVDNIVERLVPHRVKLVR--IGHP 259
LL GPPGTGKT + + G+ + NI++ +I + K V+
Sbjct: 426 LLFGPPGTGKTMLAKAV---ATEAGANFI-----NISMSSITSKWFGEGEKYVKAVFSLA 581
Query: 260 ARLLPQVV-DSALDAQVLRGDNSGLANDIRKEMK---VLNGKLLKTKEK 304
+++ P V+ +D+ + R +N G +RK MK ++N L+TK+K
Sbjct: 582 SKIAPSVIFVDEVDSMLGRRENPGEHEAMRK-MKNEFMVNWDGLRTKDK 725
>TC230029 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 {Arabidopsis
thaliana;} , partial (47%)
Length = 687
Score = 30.8 bits (68), Expect = 1.9
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +1
Query: 202 LLHGPPGTGKTTTVVEIILQ 221
L +GPPGTGKTTT + I Q
Sbjct: 349 LFYGPPGTGKTTTALAIAHQ 408
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.133 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,050,941
Number of Sequences: 63676
Number of extensions: 233444
Number of successful extensions: 1366
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 1350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1362
length of query: 633
length of database: 12,639,632
effective HSP length: 103
effective length of query: 530
effective length of database: 6,081,004
effective search space: 3222932120
effective search space used: 3222932120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC143341.10