BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142143.6 - phase: 0 /pseudo
(175 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI972659 69 9e-13
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 64 3e-11
AI496436 62 2e-10
CO982349 46 8e-06
TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {A... 35 0.014
TC234877 32 0.15
BI698930 32 0.20
AW757033 28 2.2
TC203544 similar to GB|AAN28739.1|23505759|AY143800 At2g23090/F2... 27 3.8
TC215334 similar to UP|P93344 (P93344) Aldehyde dehydrogenase (N... 27 4.9
TC227884 27 6.4
TC227885 27 6.4
TC218007 similar to UP|Q940M8 (Q940M8) At1g73650/F25P22_7, parti... 27 6.4
TC205666 26 8.4
CF808357 26 8.4
CO981465 26 8.4
>BI972659
Length = 453
Score = 69.3 bits (168), Expect = 9e-13
Identities = 33/74 (44%), Positives = 50/74 (66%), Gaps = 1/74 (1%)
Frame = +1
Query: 9 FLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRCS-KFLSFGGRMVLFKFVLSFIHVYALSF 67
F YLG+PI S M WEP++++ K +LS+ + K+LS GGR+ L K VL+ + +Y LSF
Sbjct: 178 FHYLGMPIAVKASSRMVWEPLINKFKAKLSKWNQKYLSMGGRVTLIKSVLNALPIYLLSF 357
Query: 68 FKVPSGIISSMKSI 81
FK+P I+ + S+
Sbjct: 358 FKIPQRIVDKLVSL 399
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 64.3 bits (155), Expect = 3e-11
Identities = 28/74 (37%), Positives = 48/74 (64%), Gaps = 1/74 (1%)
Frame = +1
Query: 9 FLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRCS-KFLSFGGRMVLFKFVLSFIHVYALSF 67
F+YLG+PIG + R W+ ++ + +++LS+ + +SFGGR+ L K VL+ I +Y SF
Sbjct: 592 FIYLGIPIGANSRRCHMWDSIIRKCERKLSKWK*RHISFGGRVTLIKSVLTSIPIYFFSF 771
Query: 68 FKVPSGIISSMKSI 81
F+VP ++ + I
Sbjct: 772 FRVPQSVVDKLVKI 813
>AI496436
Length = 414
Score = 61.6 bits (148), Expect = 2e-10
Identities = 27/68 (39%), Positives = 46/68 (66%), Gaps = 1/68 (1%)
Frame = +1
Query: 9 FLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRCS-KFLSFGGRMVLFKFVLSFIHVYALSF 67
F+YLG+PIG + WEPV+ + +++L+ K++SFGGR+ L VL+ + +Y LSF
Sbjct: 139 FVYLGIPIGANSRHSDVWEPVVRKCERKLASWKQKYVSFGGRVTLINSVLTALPIYLLSF 318
Query: 68 FKVPSGII 75
F++P ++
Sbjct: 319 FRIPDKVV 342
>CO982349
Length = 795
Score = 46.2 bits (108), Expect = 8e-06
Identities = 22/74 (29%), Positives = 43/74 (57%), Gaps = 1/74 (1%)
Frame = +3
Query: 9 FLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRCS-KFLSFGGRMVLFKFVLSFIHVYALSF 67
F YLG+PI + +P++ + + +L+R + +S GGR+ L +L+ + +Y SF
Sbjct: 495 FSYLGIPIEANPRCREI*DPIIRKCETKLARWKQRHISLGGRVTLINAILTALPIYFFSF 674
Query: 68 FKVPSGIISSMKSI 81
F+ PS ++ + +I
Sbjct: 675 FRAPSKVVQKLVNI 716
>TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {Arabidopsis
thaliana;} , partial (18%)
Length = 759
Score = 35.4 bits (80), Expect = 0.014
Identities = 15/35 (42%), Positives = 23/35 (64%)
Frame = +2
Query: 47 GGRMVLFKFVLSFIHVYALSFFKVPSGIISSMKSI 81
GGR+ L K VLS + + LSFFK+P I+ + ++
Sbjct: 5 GGRVTLIKSVLSALPIXXLSFFKIPQRIVDKLVTL 109
>TC234877
Length = 534
Score = 32.0 bits (71), Expect = 0.15
Identities = 20/78 (25%), Positives = 39/78 (49%), Gaps = 1/78 (1%)
Frame = +1
Query: 4 MGKVIFLYLGLPIGGDLSRLMFWEPVLDRIKKRL-SRCSKFLSFGGRMVLFKFVLSFIHV 62
+G + F+YL +P+ + R + P+ DRIK L S LSF G + L ++ + +
Sbjct: 28 VGSLPFIYLSVPLFQEKPRPVHLRPIADRIKAELQSWKGNLLSFMGCVQLIISIIDGMLL 207
Query: 63 YALSFFKVPSGIISSMKS 80
+ + P ++ M++
Sbjct: 208 *SFHLYSWPKALLIEMQT 261
>BI698930
Length = 384
Score = 31.6 bits (70), Expect = 0.20
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +3
Query: 44 LSFGGRMVLFKFVLSFIHVYALSFFKVPSGI 74
LSF GR L +LS + +++ SFFKVP +
Sbjct: 105 LSFAGRTCLINSMLSSLPLFSFSFFKVPLNV 197
>AW757033
Length = 441
Score = 28.1 bits (61), Expect = 2.2
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = -2
Query: 9 FLYLGLPIGGDLSRLMFWEPVL 30
F YLG+PIG + R W+P++
Sbjct: 71 FSYLGIPIGANPRRRETWDPII 6
>TC203544 similar to GB|AAN28739.1|23505759|AY143800 At2g23090/F21P24.15
{Arabidopsis thaliana;} , complete
Length = 951
Score = 27.3 bits (59), Expect = 3.8
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = +3
Query: 62 VYALSFFKVPSGIISSMKSIFIKKKKKKLGCV*GS*ENLMG 102
VY SFF +PS + S++ + + +KKK C+ G ++ MG
Sbjct: 114 VYIRSFFFLPSFLPSTLSTQIL*EKKKLGFCISGQTDSDMG 236
>TC215334 similar to UP|P93344 (P93344) Aldehyde dehydrogenase (NAD+) ,
partial (92%)
Length = 2249
Score = 26.9 bits (58), Expect = 4.9
Identities = 19/49 (38%), Positives = 26/49 (52%)
Frame = -2
Query: 19 DLSRLMFWEPVLDRIKKRLSRCSKFLSFGGRMVLFKFVLSFIHVYALSF 67
DLSRL W P++ ++ CSK + +VLFK +S Y LSF
Sbjct: 760 DLSRLFIWLPIIPSLE-----CSKVI-----IVLFK-QISSAQQYPLSF 647
>TC227884
Length = 1152
Score = 26.6 bits (57), Expect = 6.4
Identities = 25/91 (27%), Positives = 38/91 (41%), Gaps = 9/91 (9%)
Frame = +3
Query: 8 IFLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRCSKFLSFGGRMVLF---------KFVLS 58
IFL+ +P W+ ++ LS + F++FGG MV K VL
Sbjct: 618 IFLFARIP--------QIWQNFSNKSTGELSFITSFMNFGGSMVRVFTTIQESAPKSVLL 773
Query: 59 FIHVYALSFFKVPSGIISSMKSIFIKKKKKK 89
+ + F + S II+ K K+KK K
Sbjct: 774 GYAIGVATNFTILSQIIAYQKPQVGKEKKTK 866
>TC227885
Length = 656
Score = 26.6 bits (57), Expect = 6.4
Identities = 25/91 (27%), Positives = 38/91 (41%), Gaps = 9/91 (9%)
Frame = +1
Query: 8 IFLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRCSKFLSFGGRMVLF---------KFVLS 58
IFL+ +P W+ ++ LS + F++FGG MV K VL
Sbjct: 100 IFLFARIP--------QIWQNFSNKSTGELSFITSFMNFGGSMVRVFTTIQENAPKSVLL 255
Query: 59 FIHVYALSFFKVPSGIISSMKSIFIKKKKKK 89
+ + F + S II+ K K+KK K
Sbjct: 256 GYAIGVATNFTILSQIIAYQKPQVGKEKKTK 348
>TC218007 similar to UP|Q940M8 (Q940M8) At1g73650/F25P22_7, partial (12%)
Length = 772
Score = 26.6 bits (57), Expect = 6.4
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = -3
Query: 47 GGRMVLFKFVLSFIHVYALSFF 68
GG++ F F +S+IH++ +F+
Sbjct: 68 GGKLTWFSFCISYIHLHCQTFY 3
>TC205666
Length = 488
Score = 26.2 bits (56), Expect = 8.4
Identities = 11/32 (34%), Positives = 18/32 (55%)
Frame = -1
Query: 40 CSKFLSFGGRMVLFKFVLSFIHVYALSFFKVP 71
C+ FL G + K +++F+HV +FF P
Sbjct: 329 CTIFLRRGNTNMQNKGIITFLHVILCTFFNYP 234
>CF808357
Length = 616
Score = 26.2 bits (56), Expect = 8.4
Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 5/37 (13%)
Frame = +3
Query: 58 SFIHVYALS-----FFKVPSGIISSMKSIFIKKKKKK 89
SF+++ AL FF V ++SS K + KKKKKK
Sbjct: 42 SFLYLVALRMLLSLFFNVLF*LLSS*KHLLFKKKKKK 152
>CO981465
Length = 742
Score = 26.2 bits (56), Expect = 8.4
Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 3/50 (6%)
Frame = -1
Query: 40 CSKFLSFGGRMVLFKFVLSF---IHVYALSFFKVPSGIISSMKSIFIKKK 86
C + F F+F +S IH+ LSFF P+ I S +I +K K
Sbjct: 280 CFSHVKFLCHTFAFRFNISTVYPIHIKELSFFFFPNPIASYYYAIILKHK 131
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.357 0.167 0.590
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,456,964
Number of Sequences: 63676
Number of extensions: 95939
Number of successful extensions: 1527
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1511
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1520
length of query: 175
length of database: 12,639,632
effective HSP length: 91
effective length of query: 84
effective length of database: 6,845,116
effective search space: 574989744
effective search space used: 574989744
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC142143.6