BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140774.9 - phase: 0 /pseudo
(174 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC223705 55 2e-08
BI944018 40 4e-04
TC216559 similar to UP|O49574 (O49574) Predicted protein, partia... 35 0.023
TC216558 weakly similar to UP|O49574 (O49574) Predicted protein,... 35 0.023
TC215978 weakly similar to UP|Q8S1K2 (Q8S1K2) PDI-like protein, ... 35 0.023
TC215977 weakly similar to UP|Q6QUQ2 (Q6QUQ2) Protein disulfide ... 35 0.023
BU549990 32 0.20
TC213729 similar to UP|Q9LUH9 (Q9LUH9) Arabidopsis thaliana geno... 31 0.34
TC205047 weakly similar to UP|Q6QUQ2 (Q6QUQ2) Protein disulfide ... 30 0.75
CF808357 29 0.98
TC220226 28 2.2
TC220056 weakly similar to UP|ETFD_HUMAN (Q16134) Electron trans... 28 2.2
BM528730 28 2.2
TC217395 similar to UP|Q84VA4 (Q84VA4) DNAJ-like protein (Fragme... 28 2.9
TC227413 similar to UP|Q7PS25 (Q7PS25) ENSANGP00000020753 (Fragm... 25 3.3
TC223869 27 3.7
TC215184 homologue to UP|Q9XIS5 (Q9XIS5) Starch branching enzyme... 27 3.7
TC224891 homologue to UP|Q6JJ29 (Q6JJ29) Prephenate dehydratase,... 27 3.7
TC214182 27 3.7
TC206707 27 4.9
>TC223705
Length = 918
Score = 55.1 bits (131), Expect = 2e-08
Identities = 26/66 (39%), Positives = 37/66 (55%)
Frame = +3
Query: 72 HPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFCALAPPALKAHPFHSQHAM 131
H QH L+ F C GCKE G G R+ C C++ LH C++ P+L HPF+ + +
Sbjct: 3 HKQHKLAFDYSESPFKCDGCKELGIGSRYKCSICDFDLHTHCSIPSPSL-FHPFYPKCSF 179
Query: 132 LFHSKP 137
F S+P
Sbjct: 180 HFLSQP 197
>BI944018
Length = 409
Score = 40.4 bits (93), Expect = 4e-04
Identities = 17/46 (36%), Positives = 25/46 (53%)
Frame = +3
Query: 92 KEYGSGKRFVCQQCNYQLHDFCALAPPALKAHPFHSQHAMLFHSKP 137
+E G G R+ C C++ LH CA+ HPF+++ F SKP
Sbjct: 3 REVGIGSRYKCSICDFDLHMHCAMITSPTLHHPFYTKCNFQFMSKP 140
>TC216559 similar to UP|O49574 (O49574) Predicted protein, partial (85%)
Length = 866
Score = 34.7 bits (78), Expect = 0.023
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2
Query: 74 QHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFC 113
+H L + D+ ++C CK+ G F C C+Y LH C
Sbjct: 587 EHVLKL-DMAKAYVCDSCKKQGKFWTFSCDVCDYDLHPSC 703
>TC216558 weakly similar to UP|O49574 (O49574) Predicted protein, partial
(64%)
Length = 743
Score = 34.7 bits (78), Expect = 0.023
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +3
Query: 74 QHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFC 113
+H L + D+ ++C CK+ G F C C+Y LH C
Sbjct: 330 EHVLKL-DMAKAYVCDSCKKQGKFWTFSCDVCDYDLHPSC 446
>TC215978 weakly similar to UP|Q8S1K2 (Q8S1K2) PDI-like protein, partial (34%)
Length = 1554
Score = 34.7 bits (78), Expect = 0.023
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +3
Query: 86 FICVGCKEYGSGKRFVCQQCNYQLHDFC 113
FIC C E GS + C QC Y++H C
Sbjct: 1167 FICCVCDEQGSSWAYQCLQCGYEVHPKC 1250
>TC215977 weakly similar to UP|Q6QUQ2 (Q6QUQ2) Protein disulfide isomerase,
partial (8%)
Length = 1237
Score = 34.7 bits (78), Expect = 0.023
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +1
Query: 86 FICVGCKEYGSGKRFVCQQCNYQLHDFC 113
FIC C E GS + C QC Y++H C
Sbjct: 763 FICCVCDEQGSSWAYQCLQCGYEVHPKC 846
>BU549990
Length = 670
Score = 31.6 bits (70), Expect = 0.20
Identities = 19/58 (32%), Positives = 26/58 (44%)
Frame = +1
Query: 39 SKVTTIVTKKSPPPIEFPTSPQLIFGEEIIHFSHPQHPLSMVDLPDLFICVGCKEYGS 96
S VTT+ PPP+ PTSP + + P P S+ P+ I +G GS
Sbjct: 142 SIVTTVTIALPPPPLSLPTSPLTLL---LX*SPSPLTPTSLTTTPNTTINIGIVTIGS 306
>TC213729 similar to UP|Q9LUH9 (Q9LUH9) Arabidopsis thaliana genomic DNA,
chromosome 3, P1 clone: MWI23 (At3g22820), partial (50%)
Length = 722
Score = 30.8 bits (68), Expect = 0.34
Identities = 18/54 (33%), Positives = 25/54 (45%)
Frame = -1
Query: 108 QLHDFCALAPPALKAHPFHSQHAMLFHSKPDDKIQMRRLWQTNKRVYIPMHCMR 161
Q H + A P ++ PF+ Q L H P + +LWQT Y+ M C R
Sbjct: 464 QAHLEVSCASPLMRP-PFYLQRPSLAHFSP*VPESLYQLWQTWDTEYLNMECQR 306
>TC205047 weakly similar to UP|Q6QUQ2 (Q6QUQ2) Protein disulfide isomerase,
partial (91%)
Length = 2014
Score = 29.6 bits (65), Expect = 0.75
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +3
Query: 85 LFICVGCKEYGSGKRFVCQQCNYQLHDFCAL 115
++ C C E G + C C++ LH CAL
Sbjct: 1584 VYYCDACNEEGHIWSYYCGDCDFDLHPKCAL 1676
>CF808357
Length = 616
Score = 29.3 bits (64), Expect = 0.98
Identities = 13/24 (54%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +2
Query: 91 CKEYGSGKRFVCQQC-NYQLHDFC 113
C GSG R+VC QC N+Q+ D C
Sbjct: 467 CILMGSGNRWVCNQCKNFQICDRC 538
>TC220226
Length = 448
Score = 28.1 bits (61), Expect = 2.2
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 4/65 (6%)
Frame = +2
Query: 79 MVDLPDLFICV----GCKEYGSGKRFVCQQCNYQLHDFCALAPPALKAHPFHSQHAMLFH 134
++ LP + C+ GC +G + Q N Q++ A P A +HP H
Sbjct: 29 VISLPFIIFCLVLGFGCYFFGRARGRREVQTNPQVYGMPAPPPTACASHP---------H 181
Query: 135 SKPDD 139
SKPD+
Sbjct: 182 SKPDN 196
>TC220056 weakly similar to UP|ETFD_HUMAN (Q16134) Electron transfer
flavoprotein-ubiquinone oxidoreductase, mitochondrial
precursor (ETF-QO) (ETF-ubiquinone oxidoreductase) (ETF
dehydrogenase) (Electron-transferring-flavoprotein
dehydrogenase) , partial (18%)
Length = 781
Score = 28.1 bits (61), Expect = 2.2
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +2
Query: 112 FCALAPPALKAHPFHSQH 129
FC L P +K HPFH+QH
Sbjct: 590 FCCL-PQKIKPHPFHTQH 640
>BM528730
Length = 424
Score = 28.1 bits (61), Expect = 2.2
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = -2
Query: 5 KQKQVEKKTNTFLLEK 20
K+KQVEKK+NTF+ +K
Sbjct: 258 KKKQVEKKSNTFIAQK 211
>TC217395 similar to UP|Q84VA4 (Q84VA4) DNAJ-like protein (Fragment), partial
(68%)
Length = 1014
Score = 27.7 bits (60), Expect = 2.9
Identities = 9/27 (33%), Positives = 16/27 (58%)
Frame = +2
Query: 77 LSMVDLPDLFICVGCKEYGSGKRFVCQ 103
L VD P ++C G + Y + + ++CQ
Sbjct: 365 LQKVDAPSAYVCAGSRIYDATEWYICQ 445
>TC227413 similar to UP|Q7PS25 (Q7PS25) ENSANGP00000020753 (Fragment),
partial (38%)
Length = 1408
Score = 25.4 bits (54), Expect(2) = 3.3
Identities = 16/46 (34%), Positives = 21/46 (44%), Gaps = 1/46 (2%)
Frame = -1
Query: 60 QLIFGE-EIIHFSHPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQ 104
QLI + ++I H HP M L ++CV C R CQQ
Sbjct: 679 QLIHSQHQVISLCHKPHPHRMTLLAQQWLCVCC------SRCYCQQ 560
Score = 20.4 bits (41), Expect(2) = 3.3
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 4/33 (12%)
Frame = -3
Query: 38 YSKVT----TIVTKKSPPPIEFPTSPQLIFGEE 66
YS +T + +T K+PPPI LI EE
Sbjct: 800 YSPLTR*SGSFITGKTPPPILTIQRNLLISSEE 702
>TC223869
Length = 479
Score = 27.3 bits (59), Expect = 3.7
Identities = 22/74 (29%), Positives = 33/74 (43%), Gaps = 3/74 (4%)
Frame = +2
Query: 21 SPTKEYQITAPSHDDQYYSKVTTIVTKKSPPPIEFPTSPQLIFGEEIIHFSHPQHP---L 77
+P+K + T PS S ++ + T +SPP PT+ F +I+ FS P H
Sbjct: 221 TPSKPTKTTPPSSQS---S*ISALQTPQSPPWTT-PTACSTKFPNQILFFSTPWHAGMRA 388
Query: 78 SMVDLPDLFICVGC 91
M L F + C
Sbjct: 389 LMTHLEQFFCVLRC 430
>TC215184 homologue to UP|Q9XIS5 (Q9XIS5) Starch branching enzyme , partial
(16%)
Length = 1220
Score = 27.3 bits (59), Expect = 3.7
Identities = 10/24 (41%), Positives = 14/24 (57%)
Frame = +3
Query: 139 DKIQMRRLWQTNKRVYIPMHCMRF 162
DK + +LW N R+ +PMH F
Sbjct: 921 DKCESTKLWSANLRLNVPMHVSIF 992
>TC224891 homologue to UP|Q6JJ29 (Q6JJ29) Prephenate dehydratase, partial
(75%)
Length = 1993
Score = 27.3 bits (59), Expect = 3.7
Identities = 14/49 (28%), Positives = 21/49 (42%)
Frame = +3
Query: 60 QLIFGEEIIHFSHPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQ 108
Q I + HF H HP+S L LF+C + Y Q+ ++
Sbjct: 24 QAIVTHYLFHFPHHSHPIS---LSSLFLCQATR*YAESFTTYFQRAQFE 161
>TC214182
Length = 963
Score = 27.3 bits (59), Expect = 3.7
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = -1
Query: 2 MMRKQKQVEKKTNTFLLEKSPTKEYQITAPSH 33
+M+KQK+ K+ + EK+P+K + +PSH
Sbjct: 429 LMQKQKESSKRAAEY--EKNPSKALESNSPSH 340
>TC206707
Length = 624
Score = 26.9 bits (58), Expect = 4.9
Identities = 13/25 (52%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +2
Query: 84 DLFICVGCKEYGSGKRFV-CQQCNY 107
DL +C CK G G RFV Q CN+
Sbjct: 539 DL*VCAECKCNGXGLRFVSVQACNF 613
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.136 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,555,996
Number of Sequences: 63676
Number of extensions: 190016
Number of successful extensions: 1520
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1467
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1517
length of query: 174
length of database: 12,639,632
effective HSP length: 91
effective length of query: 83
effective length of database: 6,845,116
effective search space: 568144628
effective search space used: 568144628
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC140774.9