Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140721.8 + phase: 0 /pseudo
         (59 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC206635 similar to UP|Q9SC39 (Q9SC39) Germin-like protein (Frag...    58  1e-09
TC206636 homologue to UP|Q9SC39 (Q9SC39) Germin-like protein (Fr...    57  2e-09
BI427150                                                               47  2e-06
TC227306 similar to GB|AAB51752.1|1934730|ATU95036 germin-like p...    31  0.15
TC221249 weakly similar to UP|GL32_ARATH (Q9SR72) Germin-like pr...    30  0.33
TC224703 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor pr...    29  0.43
TC232400 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-int...    29  0.56
AW233823 weakly similar to SP|Q9S8P4|RHRE Rhicadhesin receptor p...    28  0.74
TC227305 similar to GB|AAB51752.1|1934730|ATU95036 germin-like p...    28  0.74
BI424039 homologue to GP|23093676|gb| CG32071-PA {Drosophila mel...    27  1.6
TC213376                                                               27  1.6
TC211313 weakly similar to UP|Q9SC38 (Q9SC38) Germin-like protei...    26  3.7
AI440812 similar to GP|11692852|gb| At1g09560 {Arabidopsis thali...    26  4.8
BI320231 weakly similar to GP|22086620|gb S-locus receptor-like ...    25  8.1

>TC206635 similar to UP|Q9SC39 (Q9SC39) Germin-like protein (Fragment),
           partial (95%)
          Length = 897

 Score = 57.8 bits (138), Expect = 1e-09
 Identities = 27/39 (69%), Positives = 34/39 (86%)
 Frame = +3

Query: 1   MKILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDG 39
           MK++YLLV++LAL S++  AYDPSPLQDFCVA K+ KDG
Sbjct: 66  MKVVYLLVALLALTSTVVSAYDPSPLQDFCVAAKE-KDG 179


>TC206636 homologue to UP|Q9SC39 (Q9SC39) Germin-like protein (Fragment),
          complete
          Length = 931

 Score = 57.0 bits (136), Expect = 2e-09
 Identities = 27/39 (69%), Positives = 33/39 (84%)
 Frame = +2

Query: 1  MKILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDG 39
          MK++Y LV+ILAL S++  AYDPSPLQDFCVA K+ KDG
Sbjct: 59 MKVVYFLVAILALTSTVVSAYDPSPLQDFCVAAKE-KDG 172


>BI427150 
          Length = 422

 Score = 47.0 bits (110), Expect = 2e-06
 Identities = 21/24 (87%), Positives = 21/24 (87%)
 Frame = +2

Query: 16 SIAFAYDPSPLQDFCVAIKDPKDG 39
          SIAFAYDPSPLQDFCVAI D K G
Sbjct: 2  SIAFAYDPSPLQDFCVAINDTKTG 73


>TC227306 similar to GB|AAB51752.1|1934730|ATU95036 germin-like protein
           {Arabidopsis thaliana;} , partial (46%)
          Length = 446

 Score = 30.8 bits (68), Expect = 0.15
 Identities = 14/29 (48%), Positives = 17/29 (58%)
 Frame = +1

Query: 5   YLLVSILALASSIAFAYDPSPLQDFCVAI 33
           + +  I   A S AFA DP  LQD CVA+
Sbjct: 118 FAIFVIFCCAISFAFASDPDTLQDLCVAL 204


>TC221249 weakly similar to UP|GL32_ARATH (Q9SR72) Germin-like protein
           subfamily 3 member 2 precursor, partial (58%)
          Length = 1000

 Score = 29.6 bits (65), Expect = 0.33
 Identities = 18/41 (43%), Positives = 25/41 (60%)
 Frame = +2

Query: 2   KILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDGDYS 42
           K+  LLV +L + +  A A DP P+ DFC+A K P D  +S
Sbjct: 95  KVAVLLVVLLFIIA--ALASDPDPVMDFCIA-KSP-DNSFS 205


>TC224703 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
           (Germin-like protein), partial (91%)
          Length = 1092

 Score = 29.3 bits (64), Expect = 0.43
 Identities = 14/28 (50%), Positives = 16/28 (57%)
 Frame = +1

Query: 5   YLLVSILALASSIAFAYDPSPLQDFCVA 32
           +L    LAL  +   A DP PLQD CVA
Sbjct: 145 FLQAVTLALVLATVSASDPDPLQDLCVA 228


>TC232400 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-interacting
           protein PHIP1, partial (8%)
          Length = 621

 Score = 28.9 bits (63), Expect = 0.56
 Identities = 14/32 (43%), Positives = 18/32 (55%)
 Frame = -1

Query: 4   LYLLVSILALASSIAFAYDPSPLQDFCVAIKD 35
           L LLV +L L +S+A    P PL  F  A+ D
Sbjct: 459 LSLLVVVLVLVNSVASGISPPPLDSFSSAVVD 364


>AW233823 weakly similar to SP|Q9S8P4|RHRE Rhicadhesin receptor precursor
          (Germin-like protein). [Garden pea] {Pisum sativum},
          partial (64%)
          Length = 454

 Score = 28.5 bits (62), Expect = 0.74
 Identities = 13/22 (59%), Positives = 14/22 (63%)
 Frame = +2

Query: 11 LALASSIAFAYDPSPLQDFCVA 32
          LAL  +   A DP PLQD CVA
Sbjct: 8  LALVLATVSASDPDPLQDLCVA 73


>TC227305 similar to GB|AAB51752.1|1934730|ATU95036 germin-like protein
           {Arabidopsis thaliana;} , partial (96%)
          Length = 1034

 Score = 28.5 bits (62), Expect = 0.74
 Identities = 12/29 (41%), Positives = 16/29 (54%)
 Frame = +3

Query: 5   YLLVSILALASSIAFAYDPSPLQDFCVAI 33
           + ++     A S  FA DP  LQD CVA+
Sbjct: 126 FAILVFFCCAISFTFASDPDTLQDLCVAL 212


>BI424039 homologue to GP|23093676|gb| CG32071-PA {Drosophila melanogaster},
           partial (6%)
          Length = 380

 Score = 27.3 bits (59), Expect = 1.6
 Identities = 10/14 (71%), Positives = 12/14 (85%)
 Frame = +2

Query: 45  THLVYLWLALILPL 58
           THL Y+W AL+LPL
Sbjct: 296 THLCYIWRALLLPL 337


>TC213376 
          Length = 390

 Score = 27.3 bits (59), Expect = 1.6
 Identities = 14/25 (56%), Positives = 18/25 (72%), Gaps = 1/25 (4%)
 Frame = +3

Query: 26 LQDFC-VAIKDPKDGDYSDLTHLVY 49
          LQ  C  AI++PK+GDYS   HL+Y
Sbjct: 51 LQFLCYAAIRNPKEGDYS---HLIY 116


>TC211313 weakly similar to UP|Q9SC38 (Q9SC38) Germin-like protein
           (Fragment), partial (88%)
          Length = 737

 Score = 26.2 bits (56), Expect = 3.7
 Identities = 11/17 (64%), Positives = 13/17 (75%)
 Frame = +3

Query: 40  DYSDLTHLVYLWLALIL 56
           +Y   THLVYLWLA I+
Sbjct: 114 NYQG*THLVYLWLA*IM 164


>AI440812 similar to GP|11692852|gb| At1g09560 {Arabidopsis thaliana},
           partial (23%)
          Length = 367

 Score = 25.8 bits (55), Expect = 4.8
 Identities = 15/32 (46%), Positives = 20/32 (61%)
 Frame = +2

Query: 1   MKILYLLVSILALASSIAFAYDPSPLQDFCVA 32
           M  L LLV++  L ++ A + DP  LQD CVA
Sbjct: 161 MANLLLLVTLAQLLAT-AISSDPDLLQDLCVA 253


>BI320231 weakly similar to GP|22086620|gb S-locus receptor-like kinase
          RLK14 {Oryza sativa}, partial (15%)
          Length = 422

 Score = 25.0 bits (53), Expect = 8.1
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +3

Query: 26 LQDFCVAIKDPKDGDYSDLTHLVYLWL 52
          L+DFC++IK P  G + +++  V  +L
Sbjct: 21 LEDFCISIKIPD*GLFIEISKQVMFYL 101


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.328    0.145    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,241,250
Number of Sequences: 63676
Number of extensions: 35559
Number of successful extensions: 224
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of query: 59
length of database: 12,639,632
effective HSP length: 35
effective length of query: 24
effective length of database: 10,410,972
effective search space: 249863328
effective search space used: 249863328
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)


Medicago: description of AC140721.8