BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140721.12 - phase: 0
(109 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213121 76 2e-15
CD402931 similar to GP|9081793|dbj P0489A01.23 {Oryza sativa (ja... 33 0.016
TC208075 similar to UP|Q9FPS8 (Q9FPS8) Ubiquitin-specific protea... 28 0.51
TC208074 similar to UP|Q754G3 (Q754G3) AFR107Wp, partial (6%) 28 0.51
TC208021 weakly similar to UP|Q8YSP4 (Q8YSP4) All3040 protein, p... 28 0.87
TC215241 homologue to UP|Q6WV72 (Q6WV72) Histone H4, complete 26 2.5
TC206134 26 3.3
BU547047 26 3.3
TC227823 homologue to UP|Q9AT07 (Q9AT07) ADP-glucose pyrophospho... 25 4.3
AW311473 25 4.3
BI893296 similar to GP|21553404|gb 26S proteasome regulatory par... 25 5.6
TC205434 homologue to UP|PRS6_ARATH (Q9SEI4) 26S protease regula... 25 5.6
TC225592 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 ... 25 5.6
TC216335 similar to GB|AAO42967.1|28416873|BT004721 At5g19875 {A... 25 5.6
BE211146 similar to PIR|S17434|S174 translation elongation facto... 25 7.4
TC225775 similar to GB|AAP21361.1|30102886|BT006553 At4g39880 {A... 24 9.6
TC206135 24 9.6
TC218826 weakly similar to UP|Q6NQK3 (Q6NQK3) At1g12450, partial... 24 9.6
>TC213121
Length = 713
Score = 76.3 bits (186), Expect = 2e-15
Identities = 40/84 (47%), Positives = 50/84 (58%)
Frame = +2
Query: 12 FTNKLIVDELNYNKDELTKTQTDMLLMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTG 71
+ NKLI +EL Y+KD L + LTDE + +KIM GG +FLYGYGGTG
Sbjct: 329 YGNKLIYNELAYDKDILAA*FSRCYHSLTDEHASIFNKIMHMIATQSGGVYFLYGYGGTG 508
Query: 72 KTFIWKTSLAVVRSKGLIVLNAAS 95
KTF+WKT + + S IVL AS
Sbjct: 509 KTFVWKTLSSTIHSNSGIVLTMAS 580
>CD402931 similar to GP|9081793|dbj P0489A01.23 {Oryza sativa (japonica
cultivar-group)}, partial (5%)
Length = 617
Score = 33.5 bits (75), Expect = 0.016
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +2
Query: 69 GTGKTFIWKTSLAVVRSKGLIVLNAASRV 97
GT KTF+WKT A +RSKGL+ + R+
Sbjct: 11 GTSKTFLWKTLSAGMRSKGLMSSSCLKRL 97
>TC208075 similar to UP|Q9FPS8 (Q9FPS8) Ubiquitin-specific protease 16,
partial (4%)
Length = 694
Score = 28.5 bits (62), Expect = 0.51
Identities = 14/55 (25%), Positives = 27/55 (48%)
Frame = +3
Query: 25 KDELTKTQTDMLLMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTS 79
+++ + + ++ +DE C D DS DD + L+G G G + +W+ S
Sbjct: 240 EEDSSSDNSSLISSNSDEGSCSTDSTRDSTSTDDFSEY-LFGDSGNGWSSVWRNS 401
>TC208074 similar to UP|Q754G3 (Q754G3) AFR107Wp, partial (6%)
Length = 759
Score = 28.5 bits (62), Expect = 0.51
Identities = 14/55 (25%), Positives = 27/55 (48%)
Frame = +3
Query: 25 KDELTKTQTDMLLMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTS 79
+++ + + ++ +DE C D DS DD + L+G G G + +W+ S
Sbjct: 87 EEDSSSDNSSLISSNSDEGSCSTDSTRDSTSTDDFSEY-LFGDSGNGWSSVWRNS 248
>TC208021 weakly similar to UP|Q8YSP4 (Q8YSP4) All3040 protein, partial (40%)
Length = 1747
Score = 27.7 bits (60), Expect = 0.87
Identities = 20/63 (31%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Frame = +3
Query: 47 HDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKGL--IVLNAASRVYREFYWQ 104
H K++D+ G + GF F+ TGK I+ TS A S + + L +S Y + Q
Sbjct: 1551 HSKLLDAIGI*NQGFIFMAPRFHTGKD*IFSTSRAKTTSWSITYVWLFTSSMFYLALFIQ 1730
Query: 105 QTL 107
+L
Sbjct: 1731 FSL 1739
>TC215241 homologue to UP|Q6WV72 (Q6WV72) Histone H4, complete
Length = 707
Score = 26.2 bits (56), Expect = 2.5
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 52 DSKGFDDGGFFFLYGYGG 69
+ +G D G FFF++ YGG
Sbjct: 508 NKRGMDWGSFFFIFFYGG 561
>TC206134
Length = 789
Score = 25.8 bits (55), Expect = 3.3
Identities = 17/65 (26%), Positives = 28/65 (42%)
Frame = +3
Query: 18 VDELNYNKDELTKTQTDMLLMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWK 77
V E N N+ + T + L+D+ + + +MD FD + YGG +W
Sbjct: 249 VKEDNKNEFDKTVIEDTKEEELSDDGQGLSFDLMDKLNFDTDDTGSIVLYGGGALVALWL 428
Query: 78 TSLAV 82
TS +
Sbjct: 429 TSAVI 443
>BU547047
Length = 559
Score = 25.8 bits (55), Expect = 3.3
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = -2
Query: 52 DSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKGLIVLNAASRVYREFYWQQT 106
+ + DG FF + Y + WK AVV + L L S + F+ Q+T
Sbjct: 411 EQENSQDGESFFTHSYNIMAQR--WKQLRAVVEAGDLFTLPQFSPAFCTFFGQET 253
>TC227823 homologue to UP|Q9AT07 (Q9AT07) ADP-glucose pyrophosphorylase large
subunit CagpL2, partial (25%)
Length = 1272
Score = 25.4 bits (54), Expect = 4.3
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2
Query: 2 PRPNTIDMPTFTNKLIVDEL 21
P N+ D+ TFT+ +IVDE+
Sbjct: 854 PLYNSFDISTFTSDIIVDEM 795
>AW311473
Length = 186
Score = 25.4 bits (54), Expect = 4.3
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3
Query: 2 PRPNTIDMPTFTNKLIVDEL 21
P N+ D+ TFT+ +IVDE+
Sbjct: 27 PLYNSFDISTFTSDIIVDEM 86
>BI893296 similar to GP|21553404|gb 26S proteasome regulatory particle chain
RPT6-like protein {Arabidopsis thaliana}, partial (32%)
Length = 398
Score = 25.0 bits (53), Expect = 5.6
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 64 LYGYGGTGKTFIWKTSLAVVRSKGLIVLN 92
LYG GTGKT + K A+ + G + +N
Sbjct: 106 LYGPPGTGKTMLAK---AIAKESGAVFIN 183
>TC205434 homologue to UP|PRS6_ARATH (Q9SEI4) 26S protease regulatory subunit
6B homolog (26S proteasome AAA-ATPase subunit RPT3)
(Regulatory particle triple-A ATPase subunit 3), partial
(96%)
Length = 1573
Score = 25.0 bits (53), Expect = 5.6
Identities = 16/46 (34%), Positives = 21/46 (44%), Gaps = 5/46 (10%)
Frame = +1
Query: 47 HDKIMDSKGFDDGGFFFLYGYGGTGKTFIWK-----TSLAVVRSKG 87
H ++ G D LYG GTGKT + K T+ A +R G
Sbjct: 643 HHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANHTTAAFIRVVG 780
>TC225592 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
thaliana;} , partial (94%)
Length = 1612
Score = 25.0 bits (53), Expect = 5.6
Identities = 18/65 (27%), Positives = 28/65 (42%), Gaps = 9/65 (13%)
Frame = +3
Query: 47 HDKIMDSKGFDDGGFFFLYGYGGTGKTFIWK-----TSLAVVRSKGLIV----LNAASRV 97
H ++ +S G LYG GTGKT + + T +R G + + SR+
Sbjct: 645 HPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSRM 824
Query: 98 YREFY 102
RE +
Sbjct: 825 VRELF 839
>TC216335 similar to GB|AAO42967.1|28416873|BT004721 At5g19875 {Arabidopsis
thaliana;} , partial (39%)
Length = 743
Score = 25.0 bits (53), Expect = 5.6
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Frame = +3
Query: 50 IMDSKGFDDGGFFFLY--GYGGTGKTFIWKTSLAV 82
++ S+GF D FFFLY + +IW T LA+
Sbjct: 603 LVSSQGFSDFIFFFLYLFVFVEIKGIYIWCTVLAI 707
>BE211146 similar to PIR|S17434|S174 translation elongation factor eEF-1
alpha chain (gene tefS1) - soybean, partial (17%)
Length = 241
Score = 24.6 bits (52), Expect = 7.4
Identities = 11/41 (26%), Positives = 23/41 (55%)
Frame = -1
Query: 50 IMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRSKGLIV 90
I+DSK G + + G G+ ++ KTS+ +++ G ++
Sbjct: 148 ILDSKVIKWGVIRESFNHHGFGRNYLNKTSITILQKLGFLL 26
>TC225775 similar to GB|AAP21361.1|30102886|BT006553 At4g39880 {Arabidopsis
thaliana;} , partial (61%)
Length = 1166
Score = 24.3 bits (51), Expect = 9.6
Identities = 16/52 (30%), Positives = 25/52 (47%)
Frame = -1
Query: 34 DMLLMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSLAVVRS 85
D LL+L D+ V + +GF+ FF+ G+ T + +L V RS
Sbjct: 773 DPLLVLIDDSDRVEIRGNGERGFEGDVGFFVVGFCNQQATTLLLLALHVERS 618
>TC206135
Length = 784
Score = 24.3 bits (51), Expect = 9.6
Identities = 19/62 (30%), Positives = 25/62 (39%), Gaps = 3/62 (4%)
Frame = +3
Query: 24 NKDELTKT---QTDMLLMLTDEQRCVHDKIMDSKGFDDGGFFFLYGYGGTGKTFIWKTSL 80
NK+E KT T L D Q D ++D FD + YGG +W TS
Sbjct: 276 NKNEFDKTVIEDTKQDLFDDDGQGLSFD-LLDKLNFDTDDTGSIVVYGGGALVALWLTSA 452
Query: 81 AV 82
+
Sbjct: 453 VI 458
>TC218826 weakly similar to UP|Q6NQK3 (Q6NQK3) At1g12450, partial (75%)
Length = 1078
Score = 24.3 bits (51), Expect = 9.6
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -1
Query: 40 TDEQRCVHDKIMDSKGFDDGGFFFLYGYGG 69
TDE +H +I++ K F D L YGG
Sbjct: 466 TDETSSLHQQILE*KPFSDTSPATLQFYGG 377
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.140 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,410,927
Number of Sequences: 63676
Number of extensions: 46017
Number of successful extensions: 221
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of query: 109
length of database: 12,639,632
effective HSP length: 85
effective length of query: 24
effective length of database: 7,227,172
effective search space: 173452128
effective search space used: 173452128
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC140721.12