BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140720.11 - phase: 0
(139 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC234062 similar to UP|Q9FJW1 (Q9FJW1) Arabidopsis thaliana geno... 120 2e-28
TC208430 110 2e-25
BG405065 105 7e-24
TC222875 similar to GB|AAD14477.1|4249380|T2K10 ESTs gb|Z37637, ... 96 7e-21
TC220218 93 5e-20
AW350674 89 9e-19
AW705173 78 1e-15
BE807747 similar to GP|20161304|dbj B1131G08.9 {Oryza sativa (ja... 32 0.075
TC230271 32 0.098
TC208891 UP|Q6UWM8 (Q6UWM8) GNNC2999, partial (10%) 30 0.37
TC208890 similar to GB|AAO39927.1|28372890|BT003699 At3g10120 {A... 29 0.83
TC228802 similar to UP|Q6ZLD8 (Q6ZLD8) Fiber protein-like, parti... 26 5.4
TC208834 similar to UP|Q39224 (Q39224) SRG1 protein (F6I1.30/F6I... 26 5.4
BG352937 26 7.0
BM177416 similar to GP|10177733|dbj contains similarity to myosi... 25 9.2
BI699536 25 9.2
TC220291 25 9.2
TC210224 similar to UP|Q84T10 (Q84T10) Unknow protein, partial (... 25 9.2
TC214068 similar to PIR|T07370|T07370 ubiquinol-cytochrome-c red... 25 9.2
>TC234062 similar to UP|Q9FJW1 (Q9FJW1) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K9I9 (At5g67620), partial (29%)
Length = 568
Score = 120 bits (302), Expect = 2e-28
Identities = 59/69 (85%), Positives = 63/69 (90%)
Frame = +1
Query: 54 ENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNRGIQMKH 113
ENG P+K LKLLRPDDTL IGQVYRLISFEDVLKEFASKKCGKLGKLLKE+G+ GIQMKH
Sbjct: 1 ENGTPMKQLKLLRPDDTLHIGQVYRLISFEDVLKEFASKKCGKLGKLLKETGSHGIQMKH 180
Query: 114 RDFRAPNPS 122
+D RA NPS
Sbjct: 181 KDSRALNPS 207
>TC208430
Length = 933
Score = 110 bits (276), Expect = 2e-25
Identities = 59/129 (45%), Positives = 81/129 (62%), Gaps = 15/129 (11%)
Frame = +2
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSE------ 54
MGNCQA + AT+VI +P KVER+YW VSA EVM +NP HYVAL++S+ S+
Sbjct: 86 MGNCQAVDTATLVIQQPNGKVERLYWPVSASEVMKTNPDHYVALLISTTLCSSKDNENRS 265
Query: 55 ---------NGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESG 105
N + L +KLL+P DTL++GQVYRLIS ++V+K +KK K+ + L +S
Sbjct: 266 NKSDNTNTTNPVRLTRIKLLKPTDTLVLGQVYRLISAQEVMKGLWAKKQAKMKRNLSDSA 445
Query: 106 NRGIQMKHR 114
+ MK R
Sbjct: 446 QKPNLMKER 472
>BG405065
Length = 394
Score = 105 bits (262), Expect = 7e-24
Identities = 48/56 (85%), Positives = 52/56 (92%)
Frame = +2
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENG 56
MGNCQAAE ATVVI PGNK+ER+YWS+SAHEVMNSNPGHYVALVVSSPTL +ENG
Sbjct: 227 MGNCQAAEAATVVIQHPGNKIERVYWSLSAHEVMNSNPGHYVALVVSSPTLMAENG 394
>TC222875 similar to GB|AAD14477.1|4249380|T2K10 ESTs gb|Z37637, gb|AA042498
and gb|AA042269 come from this gene. {Arabidopsis
thaliana;} , partial (57%)
Length = 563
Score = 95.5 bits (236), Expect = 7e-21
Identities = 47/104 (45%), Positives = 65/104 (62%), Gaps = 5/104 (4%)
Frame = +3
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK 60
MGNCQA + A +VI P K+ER+YW V+A EVM +NPGHYV+L++ P E K
Sbjct: 93 MGNCQAVDAAVLVIQHPCGKIERLYWPVTASEVMRTNPGHYVSLIIPLPVPPQEQNQEKK 272
Query: 61 -----HLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGK 99
+KLLRP++TL +G YRLI+ ++V+K + K K K
Sbjct: 273 TVRFTRVKLLRPNETLNLGHAYRLITTQEVMKVLKADKHAKTRK 404
>TC220218
Length = 652
Score = 92.8 bits (229), Expect = 5e-20
Identities = 51/124 (41%), Positives = 78/124 (62%), Gaps = 17/124 (13%)
Frame = +2
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSS---PT------- 50
MGNCQA + AT+VI P KV++ Y +SA VM +NPGHYVAL++S+ PT
Sbjct: 122 MGNCQAIDAATLVIQHPNGKVDKFYAPLSATHVMKTNPGHYVALLISTTLCPTKDIQNCP 301
Query: 51 ----LKSENG---MPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKE 103
+K++N + + +KLL+P DTL++GQVYRLI ++V+K K+ K+ + + E
Sbjct: 302 NKSDIKNKNANNPVRITRIKLLKPTDTLVLGQVYRLIKTQEVMKGLKEKQKAKMRQNVPE 481
Query: 104 SGNR 107
S ++
Sbjct: 482 SAHK 493
>AW350674
Length = 657
Score = 88.6 bits (218), Expect = 9e-19
Identities = 47/71 (66%), Positives = 53/71 (74%), Gaps = 8/71 (11%)
Frame = -2
Query: 70 TLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNRGIQMKHRDFRAPNPS------- 122
TL IGQVYRLISF+DV ASKKCGKLGKLLKE+G+ GIQMKH+D RA NPS
Sbjct: 656 TLHIGQVYRLISFKDVXXXXASKKCGKLGKLLKETGSHGIQMKHKDSRAXNPSRNSKSEF 477
Query: 123 -PVKVIKFHVN 132
+KVI F+VN
Sbjct: 476 GSIKVINFYVN 444
>AW705173
Length = 411
Score = 78.2 bits (191), Expect = 1e-15
Identities = 43/104 (41%), Positives = 66/104 (63%), Gaps = 9/104 (8%)
Frame = +3
Query: 10 ATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSS---PTL------KSENGMPLK 60
AT+VI KV++ Y +SA VM +NPGHYVAL++S+ PT + N + +
Sbjct: 3 ATLVIQHTNGKVDKFYAPLSATHVMKTNPGHYVALLISTTLCPTKDIQNCSNANNPVRIT 182
Query: 61 HLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKES 104
+KLL+P DTL++GQVYRLI+ ++V+K K+ K+ + + ES
Sbjct: 183 RIKLLKPTDTLVLGQVYRLITTQEVMKGLREKQKAKMRQNVPES 314
>BE807747 similar to GP|20161304|dbj B1131G08.9 {Oryza sativa (japonica
cultivar-group)}, partial (48%)
Length = 407
Score = 32.3 bits (72), Expect = 0.075
Identities = 20/68 (29%), Positives = 36/68 (52%)
Frame = +1
Query: 13 VIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLKHLKLLRPDDTLL 72
++H G +VE I +V A +VM ++P H + SSP + + + ++ PD L
Sbjct: 142 IVHSNG-RVEEISGTVKASDVMKAHPKHVLKKPCSSPADAAGISGGVHKIVVVPPDAELQ 318
Query: 73 IGQVYRLI 80
G++Y L+
Sbjct: 319 RGKIYFLM 342
>TC230271
Length = 700
Score = 32.0 bits (71), Expect = 0.098
Identities = 27/108 (25%), Positives = 47/108 (43%)
Frame = +2
Query: 13 VIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLKHLKLLRPDDTLL 72
++H G +VE I ++ A E+M ++P H V+ P+ S + + ++ PD L
Sbjct: 182 IVHSNG-RVEEISGTIKASEIMKAHPKH----VLKKPSSPSTQDGVVPKIVVVPPDADLQ 346
Query: 73 IGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNRGIQMKHRDFRAPN 120
G++Y L+ E S + SG + + H D R N
Sbjct: 347 RGKIYFLMPLPSPPSEKNSSH-----RQRSSSGKKKRKEHHNDNRNNN 475
>TC208891 UP|Q6UWM8 (Q6UWM8) GNNC2999, partial (10%)
Length = 661
Score = 30.0 bits (66), Expect = 0.37
Identities = 25/80 (31%), Positives = 34/80 (42%)
Frame = +1
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK 60
MGNC + V I + KV + EV+ GH V SE+ L+
Sbjct: 94 MGNCLVLQENVVKIVKTDGKVLEYLTPIKVEEVLIPFSGHAV----------SESLTVLR 243
Query: 61 HLKLLRPDDTLLIGQVYRLI 80
HL+ P LL GQ+Y L+
Sbjct: 244 HLE---PHTKLLRGQLYYLV 294
>TC208890 similar to GB|AAO39927.1|28372890|BT003699 At3g10120 {Arabidopsis
thaliana;} , partial (8%)
Length = 787
Score = 28.9 bits (63), Expect = 0.83
Identities = 25/80 (31%), Positives = 33/80 (41%)
Frame = +2
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK 60
MGNC + V I + KV + EV+ GH V SE+ L
Sbjct: 53 MGNCLVLQENVVKIVKTDGKVLEYKTPIKVEEVLIQFSGHAV----------SES---LT 193
Query: 61 HLKLLRPDDTLLIGQVYRLI 80
L+ L P LL GQ+Y L+
Sbjct: 194 VLRYLEPHTKLLRGQLYYLV 253
>TC228802 similar to UP|Q6ZLD8 (Q6ZLD8) Fiber protein-like, partial (21%)
Length = 873
Score = 26.2 bits (56), Expect = 5.4
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 39 GHYVALVVSSPTLKSENGMPLKHLKLLR 66
G VA VVS PTL G L HL++++
Sbjct: 296 G*IVAYVVSGPTLAVTEGRVLVHLRIMQ 379
>TC208834 similar to UP|Q39224 (Q39224) SRG1 protein (F6I1.30/F6I1.30)
(At1g17020/F6I1.30), partial (54%)
Length = 1070
Score = 26.2 bits (56), Expect = 5.4
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 7/56 (12%)
Frame = +2
Query: 79 LISFEDVLKEFASKKCGKLGKLLK-------ESGNRGIQMKHRDFRAPNPSPVKVI 127
L ++ +K+ A G +GK LK E GIQ+ ++ P P P KVI
Sbjct: 326 LEAYSREIKDLAIVIIGLMGKALKIQEREIRELFEDGIQLMRMNYYPPCPEPEKVI 493
>BG352937
Length = 486
Score = 25.8 bits (55), Expect = 7.0
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = -3
Query: 18 GNKVERIYWSVSAHEVMNSNPG 39
G KV RI W HE MN G
Sbjct: 412 GRKVTRIEWQPERHEAMNLENG 347
>BM177416 similar to GP|10177733|dbj contains similarity to myosin heavy
chain kinase~gene_id:MSG15.6 {Arabidopsis thaliana},
partial (4%)
Length = 422
Score = 25.4 bits (54), Expect = 9.2
Identities = 16/50 (32%), Positives = 23/50 (46%), Gaps = 8/50 (16%)
Frame = -1
Query: 24 IYWSVSAHEVMNSNPG--HYV------ALVVSSPTLKSENGMPLKHLKLL 65
+ W HE MN+N G H+V ++ +S T+ PL HL L
Sbjct: 398 LQWKTHHHETMNANTGIVHHVHNCC*SSIYISLVTISWTP*RPLSHLLYL 249
>BI699536
Length = 422
Score = 25.4 bits (54), Expect = 9.2
Identities = 13/44 (29%), Positives = 22/44 (49%)
Frame = +2
Query: 33 VMNSNPGHYVALVVSSPTLKSENGMPLKHLKLLRPDDTLLIGQV 76
++N NP + LV S+ L S+ +PLK+ T +G +
Sbjct: 257 IVNKNPNRFGDLVSSALGLGSKTNVPLKNAPAPNKTTTFSMGNM 388
>TC220291
Length = 437
Score = 25.4 bits (54), Expect = 9.2
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +1
Query: 105 GNRGIQMKHRDFRAPNPSPV 124
GNR +Q+ D+ P P+P+
Sbjct: 211 GNRKMQINEEDYLGPGPNPI 270
>TC210224 similar to UP|Q84T10 (Q84T10) Unknow protein, partial (19%)
Length = 7461
Score = 25.4 bits (54), Expect = 9.2
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 79 LISFEDVLKEFASKKCGKLGKLLKESGN 106
LIS E ++K++ S + +L LL +SGN
Sbjct: 4888 LISAESIVKDWESSEIVQLVSLLSKSGN 4971
>TC214068 similar to PIR|T07370|T07370 ubiquinol-cytochrome-c reductase
8.2K chain - potato {Solanum tuberosum;} , complete
Length = 748
Score = 25.4 bits (54), Expect = 9.2
Identities = 11/26 (42%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
Frame = +3
Query: 14 IHRPGNKVERIYWS-VSAHEVMNSNP 38
I PGN+V + YW+ + NSNP
Sbjct: 33 IWEPGNRVTQSYWTGIGTERRSNSNP 110
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,986,784
Number of Sequences: 63676
Number of extensions: 71704
Number of successful extensions: 329
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 325
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 325
length of query: 139
length of database: 12,639,632
effective HSP length: 87
effective length of query: 52
effective length of database: 7,099,820
effective search space: 369190640
effective search space used: 369190640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC140720.11