BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140670.5 + phase: 0 /pseudo/partial
(181 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227598 similar to UP|Q7XA68 (Q7XA68) At1g01640, partial (85%) 246 3e-66
TC227597 similar to UP|Q7XA68 (Q7XA68) At1g01640, partial (42%) 107 3e-30
TC226288 similar to UP|RK13_SPIOL (P12629) 50S ribosomal protein... 68 2e-12
AW597763 31 0.37
TC216574 similar to UP|RK4_SPIOL (O49937) 50S ribosomal protein ... 29 1.4
AW509406 weakly similar to GP|15529208|gb| At2g01710/T8O11.12 {A... 28 2.4
BI315593 weakly similar to GP|4454049|emb| 98b like protein {Ara... 28 2.4
CA852220 similar to GP|5853244|gb| ribosomal protein L16a {Emeri... 28 3.1
CK605974 28 3.1
TC203429 homologue to UP|R13A_LUPLU (O49885) 60S ribosomal prote... 27 4.0
TC203732 homologue to UP|R1AD_ARATH (Q9FKC0) 60S ribosomal prote... 27 4.0
TC211473 weakly similar to UP|Q84S70 (Q84S70) Kelch-like protein... 27 4.0
BQ610089 homologue to SP|Q9FKC0|R1AD_ 60S ribosomal protein L13a... 27 4.0
NP595172 polyprotein [Glycine max] 27 4.0
TC203302 homologue to UP|R1AD_ARATH (Q9FKC0) 60S ribosomal prote... 27 4.0
AW278183 27 5.3
AI966486 27 5.3
AW596969 similar to GP|12054507|em ribosomal protein L2 {Glycine... 27 6.9
TC213100 similar to GB|AAO42770.1|28416479|BT004524 At5g10910/T3... 27 6.9
TC204006 homologue to UP|Q9FF57 (Q9FF57) Similarity to lysophosp... 26 9.0
>TC227598 similar to UP|Q7XA68 (Q7XA68) At1g01640, partial (85%)
Length = 1262
Score = 246 bits (629), Expect = 3e-66
Identities = 134/191 (70%), Positives = 146/191 (76%), Gaps = 10/191 (5%)
Frame = +1
Query: 1 RAAAGIKRIKLDGLRWRVFDAKGQILGRLASQIATVVMGKDKPTYTPNRDDGDMCIVLNA 60
+AAAG++RI LDGLRWRVFDAKGQ+LGRLASQIATVV GKDKPTYTPNRDDGDMCIVLNA
Sbjct: 331 KAAAGLRRINLDGLRWRVFDAKGQVLGRLASQIATVVQGKDKPTYTPNRDDGDMCIVLNA 510
Query: 61 KDIAVTGRKLTDKVYYWHTGKEL*RIRWPKTLQMLFAKLFCA*FQKTTYVM--------- 111
KDI VTGRKLTDKVYYWHTG + ++ +TL+ AK +K M
Sbjct: 511 KDICVTGRKLTDKVYYWHTG-YIGHLK-QRTLKDQMAKDPTEVIRKAVLRMLPRNKLRDD 684
Query: 112 -DRKLRIFPGSEHPFGDRPLEPYVMPPRTVREMRPRARRAMIRAQKKAEQQQENAEQRQN 170
DRKLRIFPGSEHPF DRPLEPYVMPPR+VREMRPRARRAMIRAQKKAEQQQ Q
Sbjct: 685 RDRKLRIFPGSEHPFVDRPLEPYVMPPRSVREMRPRARRAMIRAQKKAEQQQ------QK 846
Query: 171 AEEMKNSKKSE 181
A+ MK K E
Sbjct: 847 ADGMKKGKNGE 879
>TC227597 similar to UP|Q7XA68 (Q7XA68) At1g01640, partial (42%)
Length = 640
Score = 107 bits (266), Expect(2) = 3e-30
Identities = 53/68 (77%), Positives = 59/68 (85%), Gaps = 2/68 (2%)
Frame = +3
Query: 112 DRKLRIFPGSEHPFGDRPLEPYVMPPRTVREMRPRARRAMIRAQKKAEQQQENAE--QRQ 169
DRKLRIFPGSEHPF DRPLEPYVMPPR+VREMRPRARRAMIRAQKKAEQQQ+ A+ + +
Sbjct: 114 DRKLRIFPGSEHPFVDRPLEPYVMPPRSVREMRPRARRAMIRAQKKAEQQQQKADGMKGK 293
Query: 170 NAEEMKNS 177
N E + S
Sbjct: 294 NGEAQEES 317
Score = 41.2 bits (95), Expect(2) = 3e-30
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = +1
Query: 79 TGKEL*RIRWPKTLQMLFAKLFCA*FQKTTYVM 111
+ K L*RI+WPKTL FAKLFC +T YVM
Sbjct: 10 SSKGL*RIKWPKTLLKSFAKLFCVCCLETNYVM 108
>TC226288 similar to UP|RK13_SPIOL (P12629) 50S ribosomal protein L13,
chloroplast precursor (CL13), partial (62%)
Length = 1054
Score = 68.2 bits (165), Expect = 2e-12
Identities = 33/66 (50%), Positives = 46/66 (69%)
Frame = +1
Query: 16 WRVFDAKGQILGRLASQIATVVMGKDKPTYTPNRDDGDMCIVLNAKDIAVTGRKLTDKVY 75
W V DA ++LGRLAS IA + GK+ TYTP+ D G IV+NA+ +AV+G+K T K+Y
Sbjct: 373 WYVVDATDKVLGRLASTIAIHIRGKNLATYTPSVDMGAFVIVVNAEKVAVSGKKRTQKLY 552
Query: 76 YWHTGK 81
H+G+
Sbjct: 553 RRHSGR 570
>AW597763
Length = 382
Score = 30.8 bits (68), Expect = 0.37
Identities = 17/57 (29%), Positives = 24/57 (41%)
Frame = -3
Query: 122 EHPFGDRPLEPYVMPPRTVREMRPRARRAMIRAQKKAEQQQENAEQRQNAEEMKNSK 178
+HP R P R + RPR RR AQ+K Q R++ E+ + K
Sbjct: 341 QHPSSQRRRRSEASPRRRIFRQRPRRRRQSSAAQRKGSTAQRT--MRRSGEQRRRKK 177
>TC216574 similar to UP|RK4_SPIOL (O49937) 50S ribosomal protein L4,
chloroplast precursor (R-protein L4), partial (74%)
Length = 1364
Score = 28.9 bits (63), Expect = 1.4
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +2
Query: 113 RKLRIFPGSEHPFGDRPLEPYVMPPRTVREMRPRARRAMIRAQKKAE 159
R+ P ++ L+P+ PPR R +RP A R + + Q + E
Sbjct: 407 RRTEALPAEKNRSRPPRLQPHAPPPRRRRHLRPEAPRLVHQDQPQGE 547
>AW509406 weakly similar to GP|15529208|gb| At2g01710/T8O11.12 {Arabidopsis
thaliana}, partial (14%)
Length = 299
Score = 28.1 bits (61), Expect = 2.4
Identities = 13/31 (41%), Positives = 17/31 (53%)
Frame = -3
Query: 120 GSEHPFGDRPLEPYVMPPRTVREMRPRARRA 150
G P G RP+ +P +T RE P A+RA
Sbjct: 273 GERVPLGRRPVNVVKIPRKTHREKTPTAKRA 181
>BI315593 weakly similar to GP|4454049|emb| 98b like protein {Arabidopsis
thaliana}, partial (13%)
Length = 421
Score = 28.1 bits (61), Expect = 2.4
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +3
Query: 138 RTVREMRPRARRAMIRAQKKAE 159
R ++ RPRAR A +RAQK AE
Sbjct: 351 RRAQKPRPRAREASVRAQKIAE 416
>CA852220 similar to GP|5853244|gb| ribosomal protein L16a {Emericella
nidulans}, partial (88%)
Length = 683
Score = 27.7 bits (60), Expect = 3.1
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +3
Query: 18 VFDAKGQILGRLASQIATVVMGKDKPT 44
V DAKG ++GRLA+ +A ++ K T
Sbjct: 69 VVDAKGHLMGRLAATVAKQLLNGQKVT 149
>CK605974
Length = 459
Score = 27.7 bits (60), Expect = 3.1
Identities = 19/40 (47%), Positives = 23/40 (57%)
Frame = -3
Query: 142 EMRPRARRAMIRAQKKAEQQQENAEQRQNAEEMKNSKKSE 181
E R RA A AQKK EQQ E A+Q + +E +NS E
Sbjct: 217 EARARAAEA---AQKKQEQQGE-AKQPETVKEPENSNNGE 110
>TC203429 homologue to UP|R13A_LUPLU (O49885) 60S ribosomal protein L13a,
complete
Length = 835
Score = 27.3 bits (59), Expect = 4.0
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = +2
Query: 18 VFDAKGQILGRLASQIATVVMGKDKPTYTPNRDDGDMCIVLNAKDIAVTGRKLTDKVYY 76
V DA+ +LGRLAS +A ++ +G +V+ ++I ++G + K+ Y
Sbjct: 86 VVDARHHMLGRLASIVAKELL------------NGQKVVVVRCEEICISGGLVRQKMKY 226
>TC203732 homologue to UP|R1AD_ARATH (Q9FKC0) 60S ribosomal protein L13a-4,
complete
Length = 1043
Score = 27.3 bits (59), Expect = 4.0
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = +3
Query: 18 VFDAKGQILGRLASQIATVVMGKDKPTYTPNRDDGDMCIVLNAKDIAVTGRKLTDKVYY 76
V DA+ +LGRLAS +A ++ +G +V+ ++I ++G + K+ Y
Sbjct: 108 VVDARHHMLGRLASIVAKELL------------NGQKVVVVRCEEICISGGLVRQKMKY 248
>TC211473 weakly similar to UP|Q84S70 (Q84S70) Kelch-like protein, partial
(19%)
Length = 940
Score = 27.3 bits (59), Expect = 4.0
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 44 TYTPNRDDGDMCIVLNAKDIAVTGRKLTDKVY 75
+Y P D+ +C LN K +++G L DK++
Sbjct: 237 SYNPVHDNWTLCPSLNQKKGSLSGAALNDKIF 332
>BQ610089 homologue to SP|Q9FKC0|R1AD_ 60S ribosomal protein L13a-4.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (44%)
Length = 415
Score = 27.3 bits (59), Expect = 4.0
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = +3
Query: 18 VFDAKGQILGRLASQIATVVMGKDKPTYTPNRDDGDMCIVLNAKDIAVTGRKLTDKVYY 76
V DA+ +LGRLAS +A ++ +G +V+ ++I ++G + K+ Y
Sbjct: 111 VVDARHHMLGRLASIVAKELL------------NGQKVVVVRCEEICISGGLVRQKMKY 251
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 27.3 bits (59), Expect = 4.0
Identities = 23/68 (33%), Positives = 32/68 (46%), Gaps = 11/68 (16%)
Frame = +1
Query: 124 PFGDRPLEPYVMPPRTVREMRP-------RARRAMIRAQKKAEQ---QQENAEQRQNA-E 172
PF +PY+ P TV EM P A R +IR + EQ Q EN Q + E
Sbjct: 4252 PFNGTAQDPYLPLPLTVTEMGPVMQPVKILASRIIIRGHNQIEQILVQWENGLQDEATWE 4431
Query: 173 EMKNSKKS 180
++++ K S
Sbjct: 4432 DIEDIKAS 4455
>TC203302 homologue to UP|R1AD_ARATH (Q9FKC0) 60S ribosomal protein L13a-4,
complete
Length = 896
Score = 27.3 bits (59), Expect = 4.0
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = +1
Query: 18 VFDAKGQILGRLASQIATVVMGKDKPTYTPNRDDGDMCIVLNAKDIAVTGRKLTDKVYY 76
V DA+ +LGRLAS +A ++ +G +V+ ++I ++G + K+ Y
Sbjct: 97 VVDARHHMLGRLASIVAKELL------------NGQKVVVVRCEEICISGGLVRQKMKY 237
>AW278183
Length = 430
Score = 26.9 bits (58), Expect = 5.3
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -3
Query: 118 FPGSEHPFGDRPLEPYVMPP 137
FP S HP RP + MPP
Sbjct: 278 FPSSRHPLSPRPTQSASMPP 219
>AI966486
Length = 199
Score = 26.9 bits (58), Expect = 5.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2
Query: 45 YTPNRDDGDMCIVLNAKDIAVTGRKL 70
Y+PN +G+ C+V N ++T KL
Sbjct: 144 YSPNSSEGNSCLVFNLPQTSITLPKL 67
>AW596969 similar to GP|12054507|em ribosomal protein L2 {Glycine max},
partial (47%)
Length = 400
Score = 26.6 bits (57), Expect = 6.9
Identities = 15/39 (38%), Positives = 20/39 (50%)
Frame = +1
Query: 113 RKLRIFPGSEHPFGDRPLEPYVMPPRTVREMRPRARRAM 151
R + I G HP P P V PPR+ R ++ RA R +
Sbjct: 178 RSVYILNGKRHPCCHEPPTP*VHPPRS-RHLQRRASRPL 291
>TC213100 similar to GB|AAO42770.1|28416479|BT004524 At5g10910/T30N20_180
{Arabidopsis thaliana;} , partial (8%)
Length = 434
Score = 26.6 bits (57), Expect = 6.9
Identities = 15/46 (32%), Positives = 21/46 (45%), Gaps = 9/46 (19%)
Frame = +1
Query: 113 RKLRIFPGSEHPFGDRPLEP---------YVMPPRTVREMRPRARR 149
+K R FPG +HP P P V PP+ + +R R +R
Sbjct: 55 QKWRCFPG*QHPHYYYPYPPNPFATPFCFLVQPPQPAQNIRTRRKR 192
>TC204006 homologue to UP|Q9FF57 (Q9FF57) Similarity to lysophosphatidic acid
acyltransferase, partial (43%)
Length = 786
Score = 26.2 bits (56), Expect = 9.0
Identities = 14/55 (25%), Positives = 24/55 (43%)
Frame = +3
Query: 122 EHPFGDRPLEPYVMPPRTVREMRPRARRAMIRAQKKAEQQQENAEQRQNAEEMKN 176
EHPF +EP ++P R PR+RR + R ++ + + +N
Sbjct: 69 EHPFQHSRIEP*LVPGR-----GPRSRRRLTRCSSSLGTSKKKRSRCRTCRRFRN 218
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.327 0.140 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,659,097
Number of Sequences: 63676
Number of extensions: 76471
Number of successful extensions: 452
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 452
length of query: 181
length of database: 12,639,632
effective HSP length: 91
effective length of query: 90
effective length of database: 6,845,116
effective search space: 616060440
effective search space used: 616060440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC140670.5