BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140546.3 + phase: 0
(266 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215893 similar to UP|Q6K674 (Q6K674) Translation initiation fa... 315 1e-86
TC218960 weakly similar to UP|Q6K674 (Q6K674) Translation initia... 258 1e-69
BE802235 218 3e-57
BG725791 156 8e-39
AI940849 60 1e-09
TC206925 homologue to UP|Q39844 (Q39844) Small GTP-binding prote... 35 0.035
TC206875 UP|Q39844 (Q39844) Small GTP-binding protein (Fragment)... 32 0.23
BG352991 32 0.23
BU550885 weakly similar to SP|P25766|RGP1 Ras-related protein RG... 30 1.5
BM528845 29 2.5
TC216412 similar to UP|Q41384 (Q41384) Protein kinase (Fragment)... 27 7.3
BE801609 27 7.3
AW396705 similar to GP|1209703|gb|A maize gl1 homolog {Arabidops... 27 9.5
>TC215893 similar to UP|Q6K674 (Q6K674) Translation initiation factor
IF-3-like, partial (58%)
Length = 1171
Score = 315 bits (807), Expect = 1e-86
Identities = 186/288 (64%), Positives = 208/288 (71%), Gaps = 22/288 (7%)
Frame = +1
Query: 1 MAGITSTTVVPVKLKASHF-----------FSNKLSIPHSLNLQ-SSPSFTLPSHATVRY 48
MAGIT++ P KL SHF F LS P+SL SS S T S+ RY
Sbjct: 106 MAGITTS---PFKL--SHFPTLFSSSDSKLFGLPLSNPYSLKFDPSSLSTTSLSYVKARY 270
Query: 49 GVNLRPSAYGGGGNFRRAPPEK-------DADDGQALDLSTLSSNTVRLIDQSQNMVGVV 101
YGGGG R+P + DD +ALDLSTL S+TVRLIDQSQNMVGVV
Sbjct: 271 --------YGGGGGPPRSPNDSRFRKSSDSDDDDKALDLSTLRSDTVRLIDQSQNMVGVV 426
Query: 102 SLDQAIRMAEDAELDLVIVSAEADPPVVRIMNYSKYRYELQKKKRDQQKKSAASRMDLKE 161
S+D AI+MAED ELDLVI+S +ADPPVVRIM+YSKYRYE QKKKR QQKKSAA+RMDLKE
Sbjct: 427 SIDDAIQMAEDVELDLVILSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAANRMDLKE 606
Query: 162 LKMGYNIDQHDYSVRLKAARKFLSDGDKVKVIVNLKGREKEFRNNAIELIRRFQNDVGKL 221
LKMGYNID+HDYSVRLKAA+KFL DG+KVKVIVNLKGRE EFRN AI LI RFQNDVG+L
Sbjct: 607 LKMGYNIDEHDYSVRLKAAQKFLKDGNKVKVIVNLKGRENEFRNMAIALITRFQNDVGEL 786
Query: 222 ATEEAKNFRDKNIFITLIPNKTEVQKPQETP---TKAATDEVSVSVEA 266
EE KNFRD+NIFI L+PNK VQK QE P K+A DEVSVS +A
Sbjct: 787 GIEETKNFRDRNIFIILVPNKVAVQKTQEPPKKRDKSAADEVSVSAQA 930
>TC218960 weakly similar to UP|Q6K674 (Q6K674) Translation initiation factor
IF-3-like, partial (76%)
Length = 1461
Score = 258 bits (660), Expect = 1e-69
Identities = 134/198 (67%), Positives = 162/198 (81%), Gaps = 3/198 (1%)
Frame = +2
Query: 71 DADDGQALDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIVSAEADPPVVR 130
D LD++ L S +VRLID +Q MVGVVS A +MA+D+ELDLVIVS +ADPPVVR
Sbjct: 194 DEQQPLGLDIAALRSASVRLIDGNQIMVGVVSKTLARQMAQDSELDLVIVSPDADPPVVR 373
Query: 131 IMNYSKYRYELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAARKFLSDGDKV 190
+M+Y+KY+YE QKKK+ QQK+SAA+RMDLKELKMGYNIDQHDYSVRLKAA+KFL DGDKV
Sbjct: 374 MMDYNKYKYEQQKKKKGQQKRSAANRMDLKELKMGYNIDQHDYSVRLKAAQKFLKDGDKV 553
Query: 191 KVIVNLKGREKEFRNNAIELIRRFQNDVGKLATEEAKNFRDKNIFITLIPNKTEVQKPQE 250
KVIVNLKGRE EFRN AIELI+RFQ+DVG+LATEE K+FRD+N+ I ++P K +QK QE
Sbjct: 554 KVIVNLKGRETEFRNIAIELIKRFQSDVGELATEETKSFRDRNMSIVMVPKKAALQKAQE 733
Query: 251 TPTK---AATDEVSVSVE 265
P + +A DEVS V+
Sbjct: 734 PPKRKDMSAADEVSAGVQ 787
>BE802235
Length = 523
Score = 218 bits (554), Expect = 3e-57
Identities = 114/169 (67%), Positives = 134/169 (78%)
Frame = +2
Query: 73 DDGQALDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIVSAEADPPVVRIM 132
DD +ALDLSTL S+TVRLIDQSQNM GVVS+D AI+MAED ELDLVI+S +A+PPVVRIM
Sbjct: 17 DDDKALDLSTLRSDTVRLIDQSQNMAGVVSIDDAIQMAEDVELDLVILSPDANPPVVRIM 196
Query: 133 NYSKYRYELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAARKFLSDGDKVKV 192
+YS+Y YE K K K +AA+RMD+KELK GY+I +HDYSVRLKAA K L+DG KV
Sbjct: 197 DYSQYIYEQHKTKIGPLKSTAANRMDVKELKTGYHIYEHDYSVRLKAAHKSLNDGHTAKV 376
Query: 193 IVNLKGREKEFRNNAIELIRRFQNDVGKLATEEAKNFRDKNIFITLIPN 241
I NLKGRE E R+ AI LI RFQNDVG+ A E FRD++IFI L+PN
Sbjct: 377 ICNLKGRENEVRSMAIALITRFQNDVGEHAVHETHIFRDRDIFIVLVPN 523
>BG725791
Length = 392
Score = 156 bits (395), Expect = 8e-39
Identities = 79/98 (80%), Positives = 86/98 (87%)
Frame = +3
Query: 121 SAEADPPVVRIMNYSKYRYELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAA 180
S +A PPVVRIM+YSKYRYE QK R QQKKSAA+RMDLKELKMGYNID+HDYSVRLKAA
Sbjct: 99 SPDAGPPVVRIMDYSKYRYEQQKTPRGQQKKSAANRMDLKELKMGYNIDEHDYSVRLKAA 278
Query: 181 RKFLSDGDKVKVIVNLKGREKEFRNNAIELIRRFQNDV 218
+KFL DG+KVKV+VNLKGRE EFRN AI LI RF NDV
Sbjct: 279 QKFLKDGNKVKVLVNLKGRENEFRNMAIALITRF*NDV 392
>AI940849
Length = 175
Score = 60.1 bits (144), Expect = 1e-09
Identities = 31/58 (53%), Positives = 42/58 (71%)
Frame = +2
Query: 78 LDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIVSAEADPPVVRIMNYS 135
LD++ L S +VRLID +Q MV V + A +MA+D+ELDL IV +ADPPV R+M Y+
Sbjct: 2 LDIAALRSRSVRLIDGNQTMV*VATKTLARQMAQDSELDLSIVYPDADPPVARMMEYN 175
>TC206925 homologue to UP|Q39844 (Q39844) Small GTP-binding protein
(Fragment), partial (95%)
Length = 1111
Score = 35.0 bits (79), Expect = 0.035
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = +1
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
EE +N DKNI I LI NK++++ ++ PT+ A +
Sbjct: 589 EELRNHADKNIVIILIGNKSDLENQRQVPTEDAKE 693
>TC206875 UP|Q39844 (Q39844) Small GTP-binding protein (Fragment), complete
Length = 871
Score = 32.3 bits (72), Expect = 0.23
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +3
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
EE +N DKNI I L NK +++ ++ PT+ A +
Sbjct: 363 EELRNHADKNIVIILTGNKCDLENQRDVPTEDAKE 467
>BG352991
Length = 397
Score = 32.3 bits (72), Expect = 0.23
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +1
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
EE +N DKNI I L NK +++ ++ PT+ A +
Sbjct: 274 EELRNHADKNIVIILTGNKCDLENQRDVPTEDAKE 378
>BU550885 weakly similar to SP|P25766|RGP1 Ras-related protein RGP1
(GTP-binding regulatory protein RGP1). [Rice] {Oryza
sativa}, partial (47%)
Length = 694
Score = 29.6 bits (65), Expect = 1.5
Identities = 12/35 (34%), Positives = 21/35 (59%)
Frame = -2
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
+E + DKNI + L+ NK+++ + PT+ A D
Sbjct: 588 DELRIHADKNILVMLVGNKSDLSSLRAVPTEVARD 484
>BM528845
Length = 366
Score = 28.9 bits (63), Expect = 2.5
Identities = 20/63 (31%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
Frame = +3
Query: 34 SSPSFTLPSHATVRYGVNLRPSAYGGGGNFRR-APPEKDADDGQALDLSTLSSNTVRLID 92
SS S ++PS +++R+ + P+A GGGG R P A G +T +++T + +
Sbjct: 138 SSFSTSMPSCSSLRHSLRPTPAAAGGGGGETR*QSPTSSARQGS----TTSTAST*KTVH 305
Query: 93 QSQ 95
SQ
Sbjct: 306 PSQ 314
>TC216412 similar to UP|Q41384 (Q41384) Protein kinase (Fragment) , partial
(20%)
Length = 897
Score = 27.3 bits (59), Expect = 7.3
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
Frame = -2
Query: 21 SNKLSIPHSLNLQSSPSFT-LPSHATVRYGVNLRPSAYGGG 60
+N+ I S+ L S+PS + S AT + +NLR ++ GGG
Sbjct: 491 ANRQKISISIELTSTPSSSHFISFATGSFPINLRGTSNGGG 369
>BE801609
Length = 424
Score = 27.3 bits (59), Expect = 7.3
Identities = 11/27 (40%), Positives = 19/27 (69%)
Frame = -3
Query: 123 EADPPVVRIMNYSKYRYELQKKKRDQQ 149
E PP+ ++ +SKY+ E +KKKR ++
Sbjct: 398 ELIPPISVVLLHSKYKKEKRKKKRKKE 318
>AW396705 similar to GP|1209703|gb|A maize gl1 homolog {Arabidopsis
thaliana}, partial (11%)
Length = 422
Score = 26.9 bits (58), Expect = 9.5
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -2
Query: 19 FFSNKLSIPHSLNLQSSPSFTLPSHA 44
F + +S PHSL Q S + +P HA
Sbjct: 319 FSTLNISFPHSLTFQPSKACNIPGHA 242
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.313 0.130 0.350
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,068,919
Number of Sequences: 63676
Number of extensions: 85651
Number of successful extensions: 516
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 513
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 514
length of query: 266
length of database: 12,639,632
effective HSP length: 96
effective length of query: 170
effective length of database: 6,526,736
effective search space: 1109545120
effective search space used: 1109545120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC140546.3