BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140034.13 + phase: 0 /partial
(433 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CO980663 311 5e-85
TC216828 homologue to UP|Q6SYB1 (Q6SYB1) GTP-binding protein Typ... 221 4e-58
TC223035 similar to UP|Q73IE1 (Q73IE1) Elongation factor Tu fami... 214 8e-56
TC223636 weakly similar to UP|Q74FV3 (Q74FV3) GTP-binding protei... 196 2e-50
BE190288 similar to GP|16649147|gb GTP-binding protein typA (tyr... 102 4e-22
BE209667 similar to GP|16649147|gb| GTP-binding protein typA (ty... 63 2e-10
TC216233 GB|CAA50573.1|402753|GMFUSA translation elongation fact... 42 7e-04
TC216829 homologue to UP|Q6SYB1 (Q6SYB1) GTP-binding protein Typ... 30 1.6
TC227869 homologue to GB|AAL79581.1|18958016|AY079031 AT4g28880/... 30 2.8
TC225793 similar to GB|AAM65667.1|21593700|AY088122 J8-like prot... 28 6.2
TC225792 similar to GB|AAM65667.1|21593700|AY088122 J8-like prot... 28 6.2
CA850605 28 8.0
TC221984 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {A... 28 8.0
>CO980663
Length = 826
Score = 311 bits (796), Expect = 5e-85
Identities = 152/174 (87%), Positives = 161/174 (92%)
Frame = -3
Query: 247 NDEHVGFVMEALSHRRAEITDMGPVAGTVGRTRLCLTCPSRGLVGYRSVFSSETRGTGFM 306
NDEHVG +MEAL HRRAE+TDMGP GTVGRTRL TCPSRGLVGYRSVFSS+TRGTGFM
Sbjct: 824 NDEHVGLIMEALXHRRAEVTDMGPXXGTVGRTRLXXTCPSRGLVGYRSVFSSDTRGTGFM 645
Query: 307 HRAFLKYEKFRGPLGNVRKGVLVSVGFGSITLHALMSLEARGTLFVSPGMEAYDGMIVGE 366
HRAF YEKFRGPLGNVRKGVLVS+GFG+IT HALMSLEARGTLFV+PGMEAYDGMIVGE
Sbjct: 644 HRAFHAYEKFRGPLGNVRKGVLVSMGFGTITAHALMSLEARGTLFVTPGMEAYDGMIVGE 465
Query: 367 HSRDTDLDVNPVRAKQLTNVRSVNKDDTVKLIPPRLMTLEEAIGYVASDELIEV 420
HSRDTDLDVNPVRAK+LTN+R+ KDD VKL PPRLMTLEEAIGYVASDELIEV
Sbjct: 464 HSRDTDLDVNPVRAKELTNIRAATKDDNVKLTPPRLMTLEEAIGYVASDELIEV 303
>TC216828 homologue to UP|Q6SYB1 (Q6SYB1) GTP-binding protein TypA, partial
(52%)
Length = 1454
Score = 221 bits (564), Expect = 4e-58
Identities = 122/285 (42%), Positives = 175/285 (60%), Gaps = 1/285 (0%)
Frame = +3
Query: 137 LPTVELDPPTISMTFGVNDSPLAGRDGTHLTGGKIGDRLMAESETNLAINVLPG-MSETF 195
LP+++++ PT+ M+F +N SP GR+G ++T + DRL E E NLA+ V G ++TF
Sbjct: 180 LPSIKVEEPTVKMSFSINTSPFVGREGKYVTSRNLRDRLYRELERNLAMKVEDGETADTF 359
Query: 196 EVQGRGELQLGILIENMRREGFELSVSPPKVMYKTDKGQKLEPIEEVTIEVNDEHVGFVM 255
V GRG L + ILIENMRREG+E V PPKV+ K + LEP E T+EV +EH+G V+
Sbjct: 360 VVSGRGTLHITILIENMRREGYEFMVGPPKVINKKVNEKLLEPYEIATVEVPEEHMGAVV 539
Query: 256 EALSHRRAEITDMGPVAGTVGRTRLCLTCPSRGLVGYRSVFSSETRGTGFMHRAFLKYEK 315
E L RR ++ DM V G G T L P+RGL+G R+ + +RGT ++ F Y
Sbjct: 540 ELLGKRRGQMFDMQGV-GLEGTTLLKYKIPTRGLLGLRNSILTASRGTAILNTVFDSYGP 716
Query: 316 FRGPLGNVRKGVLVSVGFGSITLHALMSLEARGTLFVSPGMEAYDGMIVGEHSRDTDLDV 375
+ G + +G LV+ G+ T +A+ S + RG +F+ PG++ Y G IVG H R DL +
Sbjct: 717 WAGEISTRDQGSLVAFEDGTSTSYAISSSQERGQMFIGPGVDVYKGQIVGIHQRPGDLSL 896
Query: 376 NPVRAKQLTNVRSVNKDDTVKLIPPRLMTLEEAIGYVASDELIEV 420
N + K TN+RS NK+ TV L P +L++ I Y+ DEL+EV
Sbjct: 897 NVCKKKAATNIRS-NKEQTVILDTPLDYSLDDCIEYIQEDELVEV 1028
>TC223035 similar to UP|Q73IE1 (Q73IE1) Elongation factor Tu family protein,
partial (13%)
Length = 443
Score = 214 bits (544), Expect = 8e-56
Identities = 108/148 (72%), Positives = 126/148 (84%), Gaps = 4/148 (2%)
Frame = +2
Query: 170 KIGDRLMAESETNLAINVLPGMSETFEVQGRGELQLGILIENMRREGFELSVSPPKVMYK 229
+IGDRLMAE+ETNLAINVLPG+SE+FEVQGRGELQLGILIENMRREGFELSVSPPKVMYK
Sbjct: 2 RIGDRLMAEAETNLAINVLPGLSESFEVQGRGELQLGILIENMRREGFELSVSPPKVMYK 181
Query: 230 TDKGQKLEPIEEVTIEVNDEHVGFVMEALSHRRAEITDMGPVAGTVGRTRLCLTCPSRGL 289
T+ GQKLEP+E+VTIEVNDEHVG +MEALSHR+AE+TDM PV+ TVG+TRLCLTCPSR
Sbjct: 182 TESGQKLEPVEKVTIEVNDEHVGLIMEALSHRQAEVTDMDPVSRTVGQTRLCLTCPSRQN 361
Query: 290 VGYRSVFSSETRGTGF----MHRAFLKY 313
+ +F+ T F +H+ F+ Y
Sbjct: 362 I---HLFNGNTVDAWF*RNHIHKFFVSY 436
>TC223636 weakly similar to UP|Q74FV3 (Q74FV3) GTP-binding protein TypA,
partial (19%)
Length = 736
Score = 196 bits (497), Expect = 2e-50
Identities = 96/108 (88%), Positives = 102/108 (93%)
Frame = +3
Query: 313 YEKFRGPLGNVRKGVLVSVGFGSITLHALMSLEARGTLFVSPGMEAYDGMIVGEHSRDTD 372
YE FRGPLGNVRKGVLVS+GFG+IT HALMSLEARGTLFV+PGMEAYDGMIVGEHSRDTD
Sbjct: 216 YENFRGPLGNVRKGVLVSMGFGTITAHALMSLEARGTLFVTPGMEAYDGMIVGEHSRDTD 395
Query: 373 LDVNPVRAKQLTNVRSVNKDDTVKLIPPRLMTLEEAIGYVASDELIEV 420
LDVNPVRAK+LTN+R+ KDD VKL PPRLMTLEEAIGYVASDELIEV
Sbjct: 396 LDVNPVRAKELTNIRAATKDDNVKLTPPRLMTLEEAIGYVASDELIEV 539
>BE190288 similar to GP|16649147|gb GTP-binding protein typA (tyrosine
phosphorylated protein A) {Arabidopsis thaliana},
partial (42%)
Length = 505
Score = 102 bits (253), Expect = 4e-22
Identities = 67/164 (40%), Positives = 89/164 (53%), Gaps = 4/164 (2%)
Frame = +2
Query: 137 LPTVELDPPTISMTFGVNDSPLAGRDGTHLTGGKIGDRLMAESETNLAINVLPG-MSETF 195
LP+ +D PT+ M F +N SP GR+G + + L E E NLA+ V ++TF
Sbjct: 26 LPSFNVDEPTVKMPFSINTSPFVGREGKSVPIMNLTHTLYPELERNLAMKVEDSETADTF 205
Query: 196 EVQGRGELQLGILIENMRREGFELSVSPPKVMYKTDKGQKLEPIEEVTIEVNDEHVGFVM 255
RG L + ILIENM E +E V PPKV+ K + LEP E ++EV EH+G V+
Sbjct: 206 VCLVRGTLPITILIENMLGERYEFMVGPPKVINKNVDEKLLEPYEIASVEVP*EHMGAVV 385
Query: 256 EALSHRRAEITDMGPVAGTVGRTRLCLT---CPSRGLVGYRSVF 296
E L RR + DM VG R LT P+RGL+G R+ F
Sbjct: 386 ELLGKRRGTLFDM----LGVGLERTTLTQYKMPTRGLLGLRNKF 505
>BE209667 similar to GP|16649147|gb| GTP-binding protein typA (tyrosine
phosphorylated protein A) {Arabidopsis thaliana},
partial (21%)
Length = 265
Score = 63.2 bits (152), Expect = 2e-10
Identities = 32/80 (40%), Positives = 48/80 (60%)
Frame = +2
Query: 341 LMSLEARGTLFVSPGMEAYDGMIVGEHSRDTDLDVNPVRAKQLTNVRSVNKDDTVKLIPP 400
+ S + RG +F+ PG++ Y G I G H R DL +N + K TN+RS NK+ T+ L P
Sbjct: 2 ISSSQERGQMFIGPGVDVYKGQIAGIHHRPGDLSLNVCKNKAATNIRS-NKEQTLILDTP 178
Query: 401 RLMTLEEAIGYVASDELIEV 420
L++ + Y+ DEL+EV
Sbjct: 179 LDYRLDDCLRYIP*DELVEV 238
>TC216233 GB|CAA50573.1|402753|GMFUSA translation elongation factor EF-G
{Glycine max;} , complete
Length = 2424
Score = 41.6 bits (96), Expect = 7e-04
Identities = 35/148 (23%), Positives = 69/148 (45%), Gaps = 1/148 (0%)
Frame = +1
Query: 83 AEKIEDGKVVKVMKKKGTTMVVTDCAGAGDIISIAGLSSPSIGHTVTTVEIMSALPTVEL 142
A K + ++ ++++ + A AGDII++AGL G T+ + L ++
Sbjct: 1051 ANKGKKERIGRLLEMHANSREDVKVALAGDIIALAGLKDTITGETLCDPDNPIVLERMDF 1230
Query: 143 DPPTISMTFGVNDSPLAGRDGTHLTGGKIGDRLMAESETNLAINVLPGMSETFEVQGRGE 202
P I + P D + G I +A+ + + + +++T ++G GE
Sbjct: 1231 PDPVIKVAI----EPKTKADVDKMATGLI---KLAQEDPSFHFSRDEEINQTV-IEGMGE 1386
Query: 203 LQLGILIENMRRE-GFELSVSPPKVMYK 229
L L I+++ ++RE E +V P+V Y+
Sbjct: 1387 LHLEIIVDRLKREFKVEANVGAPQVNYR 1470
Score = 31.6 bits (70), Expect = 0.73
Identities = 21/67 (31%), Positives = 31/67 (45%)
Frame = +2
Query: 236 LEPIEEVTIEVNDEHVGFVMEALSHRRAEITDMGPVAGTVGRTRLCLTCPSRGLVGYRSV 295
LEPI +V + +EH+G V+ L+ RR +I G G G + P + Y S
Sbjct: 1823 LEPIMKVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG--GLKVVDALVPLAEMFQYVST 1996
Query: 296 FSSETRG 302
T+G
Sbjct: 1997 LRGMTKG 2017
>TC216829 homologue to UP|Q6SYB1 (Q6SYB1) GTP-binding protein TypA, partial
(7%)
Length = 495
Score = 30.4 bits (67), Expect = 1.6
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +1
Query: 386 VRSVNKDDTVKLIPPRLMTLEEAIGYVASDELIEV 420
VRS NK+ TV L P +L++ I Y+ DEL+EV
Sbjct: 1 VRS-NKEQTVILDTPLDYSLDDCIEYIQEDELVEV 102
>TC227869 homologue to GB|AAL79581.1|18958016|AY079031 AT4g28880/F16A16_10
{Arabidopsis thaliana;} , partial (7%)
Length = 1070
Score = 29.6 bits (65), Expect = 2.8
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = -2
Query: 18 RNNLTSLFFMLLLKKDGHPPHIPRILLPMPKICHSC 53
+N SL LKK HPPH R+ LP+ + SC
Sbjct: 748 QNRSHSLSLFPTLKK*EHPPHSTRMYLPIHLVIVSC 641
>TC225793 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;} , partial (58%)
Length = 1345
Score = 28.5 bits (62), Expect = 6.2
Identities = 18/50 (36%), Positives = 22/50 (44%)
Frame = -1
Query: 22 TSLFFMLLLKKDGHPPHIPRILLPMPKICHSCLMPLYHMSPHQMQTLMHL 71
TS LLL PP P + P + H CL +H PH + L HL
Sbjct: 829 TSSHGFLLLPIPSTPPTCPNLDRSFPSVAHRCLQ--HHNKPHTI--LPHL 692
>TC225792 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;} , partial (37%)
Length = 450
Score = 28.5 bits (62), Expect = 6.2
Identities = 18/50 (36%), Positives = 22/50 (44%)
Frame = -1
Query: 22 TSLFFMLLLKKDGHPPHIPRILLPMPKICHSCLMPLYHMSPHQMQTLMHL 71
TS LLL PP P + P + H CL +H PH + L HL
Sbjct: 306 TSSHGFLLLPIPSTPPTCPNLDRSFPSVAHRCLQ--HHNKPHTI--LPHL 169
>CA850605
Length = 291
Score = 28.1 bits (61), Expect = 8.0
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 6/52 (11%)
Frame = -3
Query: 289 LVGYRSVFSSETRGTGFMH------RAFLKYEKFRGPLGNVRKGVLVSVGFG 334
L+ Y +FS +R T F+H R F++ +FR P ++ K V S GFG
Sbjct: 163 LLLYPKLFSEGSRITFFVHLKRLWARTFIRDRRFRIPNTSICKWVNNSRGFG 8
>TC221984 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Arabidopsis
thaliana;} , partial (26%)
Length = 554
Score = 28.1 bits (61), Expect = 8.0
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -2
Query: 41 RILLPMPKICHSCLMPLYHMSPHQMQ 66
R+LLP P I HSC Y +S Q+Q
Sbjct: 484 RLLLPYPWISHSCHNEQYAVSRRQLQ 407
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.138 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,261,077
Number of Sequences: 63676
Number of extensions: 246122
Number of successful extensions: 1382
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1371
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1377
length of query: 433
length of database: 12,639,632
effective HSP length: 100
effective length of query: 333
effective length of database: 6,272,032
effective search space: 2088586656
effective search space used: 2088586656
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC140034.13