BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140030.9 - phase: 0 /pseudo
(113 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC221970 61 9e-11
TC215058 similar to UP|Q6T7D0 (Q6T7D0) Fiber protein Fb37, parti... 29 0.29
TC225388 homologue to UP|Q9ZR33 (Q9ZR33) Reversibly glycosylated... 28 0.49
TC215414 homologue to UP|O80412 (O80412) Mitochondrial phosphate... 28 0.64
TC222954 similar to UP|Q9ZVQ5 (Q9ZVQ5) Expressed protein (At2g02... 28 0.84
TC204244 weakly similar to GB|AAH70004.1|47123245|BC070004 zgc:8... 27 1.4
BQ081339 similar to GP|27817931|db P0453E05.6 {Oryza sativa (jap... 27 1.4
TC213105 similar to UP|Q9FI64 (Q9FI64) Arabidopsis thaliana geno... 27 1.9
TC217702 similar to GB|AAL06971.1|15810091|AY056083 AT4g30260/F9... 27 1.9
TC219304 homologue to UP|O22847 (O22847) Expressed protein (At2g... 26 2.4
TC204247 weakly similar to GB|AAH70004.1|47123245|BC070004 zgc:8... 26 2.4
TC220064 UP|Q8CEM2 (Q8CEM2) Mus musculus adult male testis cDNA,... 26 2.4
BF595364 26 3.2
TC215057 similar to UP|Q9SJM6 (Q9SJM6) Expressed protein (Zinc f... 26 3.2
BF424213 weakly similar to GP|21322711|em pherophorin-dz1 protei... 26 3.2
TC226926 weakly similar to UP|Q8V7C6 (Q8V7C6) ORF1 (Fragment), p... 26 3.2
TC211406 similar to GB|AAL09709.1|15982737|AY057475 AT4g23750/F9... 25 4.2
BG791134 GP|27261013|db P0434A03.29 {Oryza sativa (japonica cult... 25 4.2
BG510036 25 4.2
TC228270 25 4.2
>TC221970
Length = 365
Score = 60.8 bits (146), Expect = 9e-11
Identities = 28/36 (77%), Positives = 34/36 (93%)
Frame = +3
Query: 76 SGGGNLEMEKVFKLAREVYGKVVKKQAMRTLLELCI 111
+GGG +E EKVFK+AR+V+GKVVKKQAM+TLLELCI
Sbjct: 6 NGGGVVEREKVFKMARDVFGKVVKKQAMKTLLELCI 113
>TC215058 similar to UP|Q6T7D0 (Q6T7D0) Fiber protein Fb37, partial (45%)
Length = 610
Score = 29.3 bits (64), Expect = 0.29
Identities = 33/105 (31%), Positives = 48/105 (45%), Gaps = 8/105 (7%)
Frame = -2
Query: 5 RWKVRGKEKGYLRASFWSLYSEAYGSERCMKRWGR-RIPALDAV-----VDSI--TDVVG 56
RWK+ G R S WS YS G +R W R +P + + +D++ D
Sbjct: 447 RWKIAAP*AGN-RGSTWSTYSAWQGCQRNRNCWRRSAVPGICVLPPYFSLDTVREKDERR 271
Query: 57 GDHRVLKILMEELKRKGGGSGGGNLEMEKVFKLAREVYGKVVKKQ 101
G R+ K +EE ++G G G EK K +R + KV KK+
Sbjct: 270 G*LRLWKWKIEEEMKRGKGRGWWL*GKEKKGKQSR-I*RKVSKKR 139
>TC225388 homologue to UP|Q9ZR33 (Q9ZR33) Reversibly glycosylated
polypeptide, partial (46%)
Length = 546
Score = 28.5 bits (62), Expect = 0.49
Identities = 20/68 (29%), Positives = 34/68 (49%), Gaps = 2/68 (2%)
Frame = -3
Query: 32 RCMKRWGRRIPALDAVV-DSITDVVGGDHRVLKILMEELKRKGGGSGGGN-LEMEKVFKL 89
+C+ RRI + +AVV D + ++ GDH LKR GG G + +++ V +
Sbjct: 394 KCIDILSRRILSNEAVVIDGVDVLLLGDHVTKAATRRILKRDAGGFGSEDAIDVVAVVEF 215
Query: 90 AREVYGKV 97
E +G +
Sbjct: 214 IIEAFGDI 191
>TC215414 homologue to UP|O80412 (O80412) Mitochondrial phosphate
transporter, complete
Length = 1639
Score = 28.1 bits (61), Expect = 0.64
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2
Query: 57 GDHRVLKILMEELKRKGGGSGGGNLE 82
G+H + +E +R GGG GGG +E
Sbjct: 249 GNHEAARSGVEVFRRLGGGGGGGGVE 172
>TC222954 similar to UP|Q9ZVQ5 (Q9ZVQ5) Expressed protein
(At2g02370/T16F16.16), partial (21%)
Length = 365
Score = 27.7 bits (60), Expect = 0.84
Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +2
Query: 46 AVVDSITDVVGGDHRVLKILMEELKRKGGG-SGGGNLEMEK 85
AVV +I +V + ++ + E ++G SG GNLEMEK
Sbjct: 128 AVVTTIAFIVYAKRTLNELKIAEANQEGASVSGSGNLEMEK 250
>TC204244 weakly similar to GB|AAH70004.1|47123245|BC070004 zgc:85716 {Danio
rerio;} , partial (5%)
Length = 678
Score = 26.9 bits (58), Expect = 1.4
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 59 HRVLKILMEELKRKGGGSGGGNLEMEKV 86
+++ KIL++ L RKG SG G ++K+
Sbjct: 141 NKLKKILLQNLPRKGNRSGRGRKSLQKL 224
>BQ081339 similar to GP|27817931|db P0453E05.6 {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 420
Score = 26.9 bits (58), Expect = 1.4
Identities = 20/45 (44%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = -1
Query: 36 RWGRRIPA-LDAVVDSITDVVGGDHRVLKILMEELKRKGGGSGGG 79
RW RRI LD VV + G R L E L GGG GGG
Sbjct: 285 RWRRRISVCLDEVVVIVIGGGVGGWRRESPLGEALGSGGGGGGGG 151
>TC213105 similar to UP|Q9FI64 (Q9FI64) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K21I16, partial (26%)
Length = 416
Score = 26.6 bits (57), Expect = 1.9
Identities = 11/16 (68%), Positives = 13/16 (80%)
Frame = -1
Query: 70 KRKGGGSGGGNLEMEK 85
KR GGGSGGG+ E E+
Sbjct: 209 KRVGGGSGGGDAENEE 162
>TC217702 similar to GB|AAL06971.1|15810091|AY056083 AT4g30260/F9N11_110
{Arabidopsis thaliana;} , partial (39%)
Length = 436
Score = 26.6 bits (57), Expect = 1.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 67 EELKRKGGGSGGGNLEME 84
EE+ +GGG GGG E E
Sbjct: 316 EEMSARGGGGGGGTAETE 263
>TC219304 homologue to UP|O22847 (O22847) Expressed protein
(At2g43320/T1O24.6), partial (16%)
Length = 858
Score = 26.2 bits (56), Expect = 2.4
Identities = 8/30 (26%), Positives = 17/30 (56%)
Frame = +1
Query: 6 WKVRGKEKGYLRASFWSLYSEAYGSERCMK 35
W+ ++ G FWS +S+ +G + C++
Sbjct: 259 WRTTAEKSGR*GGHFWSSFSQGFG*QGCLE 348
>TC204247 weakly similar to GB|AAH70004.1|47123245|BC070004 zgc:85716 {Danio
rerio;} , partial (5%)
Length = 762
Score = 26.2 bits (56), Expect = 2.4
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = +3
Query: 63 KILMEELKRKGGGSGGGNLEMEKV 86
KIL++ L RKG SG G ++K+
Sbjct: 318 KILLQNLPRKGNRSGRGRKSLQKL 389
>TC220064 UP|Q8CEM2 (Q8CEM2) Mus musculus adult male testis cDNA, RIKEN
full-length enriched library, clone:1700123E07
product:protamine 1, full insert sequence, partial (22%)
Length = 461
Score = 26.2 bits (56), Expect = 2.4
Identities = 18/55 (32%), Positives = 32/55 (57%), Gaps = 1/55 (1%)
Frame = -2
Query: 32 RCMKRWGRRIPALDAVVDSITDVVG-GDHRVLKILMEELKRKGGGSGGGNLEMEK 85
R + R G+R+ + V+D +D VG GD V + ++E + GG + G +E++K
Sbjct: 454 RVLVRGGKRVFWREPVIDGESDNVGLGDEVVERGVVERI-GGGGDAEGAAVEVDK 293
>BF595364
Length = 418
Score = 25.8 bits (55), Expect = 3.2
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = -3
Query: 74 GGSGGGNLEMEKVFKLAREVYGKVVKKQ 101
GG GGG EM + F + REV G V++++
Sbjct: 179 GGGGGGGDEMAEGFDVGREV-GDVLERR 99
>TC215057 similar to UP|Q9SJM6 (Q9SJM6) Expressed protein (Zinc finger-like
protein), partial (63%)
Length = 973
Score = 25.8 bits (55), Expect = 3.2
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = -1
Query: 5 RWKVRGKEKGYLRASFWSLYSEAYGSERCMKRWGR 39
RWK+ G R S WS YS G +R W R
Sbjct: 373 RWKIAAP*AGN-RGSTWSTYSAWQGCQRNRNCWRR 272
>BF424213 weakly similar to GP|21322711|em pherophorin-dz1 protein {Volvox
carteri f. nagariensis}, partial (6%)
Length = 424
Score = 25.8 bits (55), Expect = 3.2
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = -3
Query: 56 GGDHRVLKILMEELKRKGGGSGGG 79
GG HR ++ + + +GGG GGG
Sbjct: 392 GGTHRGCRVCVVLEEEEGGGGGGG 321
>TC226926 weakly similar to UP|Q8V7C6 (Q8V7C6) ORF1 (Fragment), partial (54%)
Length = 724
Score = 25.8 bits (55), Expect = 3.2
Identities = 22/89 (24%), Positives = 35/89 (38%), Gaps = 11/89 (12%)
Frame = +1
Query: 22 SLYSEAYGSERCMKRWGRRIPALD----------AVVDSITDVVGGDHRVLKILMEELKR 71
S YS G + G ++ AL VVD+I+D H + + R
Sbjct: 382 SQYSSRGGQVSALSPTGGQVSALSPTLYEPVNRSCVVDAISDSSAPKHAIQAFHLLSTSR 561
Query: 72 KGGGSGGGNLEMEKVFK-LAREVYGKVVK 99
G S ++ ++FK L EV G ++
Sbjct: 562 DGVSSQNYSIRNREIFKCLLNEVLGSFIE 648
>TC211406 similar to GB|AAL09709.1|15982737|AY057475 AT4g23750/F9D16_220
{Arabidopsis thaliana;} , partial (24%)
Length = 1012
Score = 25.4 bits (54), Expect = 4.2
Identities = 12/41 (29%), Positives = 21/41 (50%)
Frame = +2
Query: 43 ALDAVVDSITDVVGGDHRVLKILMEELKRKGGGSGGGNLEM 83
A D+ D + R +K + E+ +GGG GGG++ +
Sbjct: 230 ATDSSSDDDEPSMSSTRRRVKNFVNEITIQGGGGGGGDVNV 352
>BG791134 GP|27261013|db P0434A03.29 {Oryza sativa (japonica
cultivar-group)}, partial (9%)
Length = 415
Score = 25.4 bits (54), Expect = 4.2
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = -1
Query: 65 LMEELKRKGGGSGGGNLEM 83
LM+ RKGGG GGG ++
Sbjct: 286 LMKRRMRKGGGGGGGERKL 230
>BG510036
Length = 428
Score = 25.4 bits (54), Expect = 4.2
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = -1
Query: 70 KRKGGGSGGGNLEMEKVFKLAREVYGKVVKKQAMRTLLELCIAA 113
+R+ GG+ N+E+ + +L E G ++K A + C A+
Sbjct: 263 RRRNGGAKPRNIEVSRDKRLFSENSGSTIEKPATDQFINWCDAS 132
>TC228270
Length = 1186
Score = 25.4 bits (54), Expect = 4.2
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 59 HRVLKILMEELKRKGGGSGGGNLEMEKVFKLAREVYGKV 97
H L + +L RKGGG GGG ME + ++Y +V
Sbjct: 1139 HCTLSNIQSKL-RKGGGGGGG---MESTM*NSADIYSRV 1035
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,894,450
Number of Sequences: 63676
Number of extensions: 59277
Number of successful extensions: 537
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 508
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 523
length of query: 113
length of database: 12,639,632
effective HSP length: 89
effective length of query: 24
effective length of database: 6,972,468
effective search space: 167339232
effective search space used: 167339232
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC140030.9