BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139854.8 - phase: 0
(304 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AI495080 86 2e-17
TC211858 51 3e-13
BQ576456 68 6e-12
CA820303 65 4e-11
CO979307 52 3e-07
TC232778 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16, partial (5%) 48 5e-06
TC234144 35 0.032
BG881451 weakly similar to PIR|D96521|D96 protein F21D18.16 [imp... 33 0.12
BF071225 weakly similar to PIR|D96521|D96 protein F21D18.16 [imp... 31 0.61
BI470376 weakly similar to GP|14090338|dbj P0638D12.5 {Oryza sat... 30 1.8
TC215161 similar to UP|Q6NQ77 (Q6NQ77) At4g05190, partial (19%) 29 3.0
TC220808 weakly similar to UP|Q9SK32 (Q9SK32) Expressed protein,... 29 3.0
BE803668 weakly similar to GP|1181599|dbj| subunit of photosyste... 28 5.1
AW279090 28 6.7
AI965377 similar to GP|21592780|gb| nucleic acid binding protein... 27 8.8
TC206438 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prot... 27 8.8
BE209913 similar to GP|10946375|gb ribulose-1 5-bisphosphate car... 27 8.8
>AI495080
Length = 407
Score = 86.3 bits (212), Expect = 2e-17
Identities = 49/122 (40%), Positives = 66/122 (53%), Gaps = 3/122 (2%)
Frame = +2
Query: 165 RALGGSVTLLTTWFLAHFPGFFSVDLNTDYLENYPVAARWKLPKGHGEGI---TYWSLLD 221
R L G +TLL W HFP + DY E P A RW K + I +Y L+
Sbjct: 2 RQLSGYITLLQCWIYEHFPSVAESTAD*DYDEASPRACRWIATKKTVKSIRTPSYRERLN 181
Query: 222 RIQLDDVCYKPYKEHREIQDFEEVFWYSGWIMCGVRRVYRHLPKRVLRQYGYVQTIPRHP 281
R+++ DVC+ Y EHRE+QDF YSG + G+ VY + P++V+RQ+GY IP P
Sbjct: 182 RLRISDVCWILYGEHREVQDFHVRSCYSGLLHWGLVAVY-YRPEKVVRQFGYTPAIPAPP 358
Query: 282 TD 283
D
Sbjct: 359 VD 364
>TC211858
Length = 524
Score = 51.2 bits (121), Expect(3) = 3e-13
Identities = 26/70 (37%), Positives = 36/70 (51%), Gaps = 3/70 (4%)
Frame = -3
Query: 177 WFLAHFPGFFSVDLNTDYLENYPVAARWKLPKGHGEGIT---YWSLLDRIQLDDVCYKPY 233
W HFP ++ Y E P A+RW K H +GIT Y + D + + DV + PY
Sbjct: 231 WIYEHFPSVHQCVIDDTY*ETSPRASRWLTSKAHMKGITGAPYRARCDALTVIDVSWLPY 52
Query: 234 KEHREIQDFE 243
EHR ++ FE
Sbjct: 51 TEHRGVRAFE 22
Score = 34.3 bits (77), Expect(3) = 3e-13
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Frame = -1
Query: 124 IELIYLTTMAD-GYAGMRNYSWGGMTLAYLYGELADACRPGHRALGGSVTLLTTWFL 179
+ ++YL D G +G Y+WG L ++Y +L +A R R + G +TLL F+
Sbjct: 470 VHVVYLDAFRDLGQSG--GYAWGVAALVHMYNQLDEASRTTTRQIVGYLTLLQVNFV 306
Score = 26.2 bits (56), Expect(3) = 3e-13
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = -2
Query: 106 YLLYLVGCLLFGDRS 120
YLL+LVGC LF ++S
Sbjct: 523 YLLHLVGCTLFANKS 479
>BQ576456
Length = 424
Score = 67.8 bits (164), Expect = 6e-12
Identities = 48/150 (32%), Positives = 67/150 (44%)
Frame = +1
Query: 4 GEMTVTLDDVACLTHLPIEGRMLDHGKKMPKHEGAAQLMTYLGVSQHEAEKICNQKYGGY 63
GE+T+TLDD+ACL HLPI G L + + E LM L VS+ EA + + Y
Sbjct: 31 GELTITLDDMACLLHLPITG-ALHRFEPLGVDEAVLLLMELLEVSREEARAKIVRAHRAY 207
Query: 64 ISYPRLRDFYTSYLGRANVLAGTEDPEELEELARVRTYCVRCYLLYLVGCLLFGDRSNKR 123
+ LR+ Y S AR VR YL +LV C LF ++S
Sbjct: 208 VRLSWLREVYQSRC-----------------QARCWIVAVRAYLFHLVDCTLFANKSATH 336
Query: 124 IELIYLTTMADGYAGMRNYSWGGMTLAYLY 153
+ +++L D Y WG L ++Y
Sbjct: 337 VHVVHLKGF*D-LC*SGGYGWGVAALVHMY 423
>CA820303
Length = 421
Score = 65.1 bits (157), Expect = 4e-11
Identities = 33/69 (47%), Positives = 44/69 (62%)
Frame = -1
Query: 215 TYWSLLDRIQLDDVCYKPYKEHREIQDFEEVFWYSGWIMCGVRRVYRHLPKRVLRQYGYV 274
+Y LDR+++ DVC+ PY EHRE+QDF YSG + G VY + +RV+RQ+GY
Sbjct: 400 SYRERLDRLRIPDVCWIPYGEHREVQDFHVRSCYSGLLCWGPVVVY-YRSERVVRQFGYT 224
Query: 275 QTIPRHPTD 283
QTIP D
Sbjct: 223 QTIPASTVD 197
>CO979307
Length = 553
Score = 52.4 bits (124), Expect = 3e-07
Identities = 43/172 (25%), Positives = 78/172 (45%), Gaps = 2/172 (1%)
Frame = -3
Query: 109 YLVGCLLFGDRSNKRIELIYLTTMADGYAGMRNYSWGGMTLAYLYGELADACRPGHRALG 168
+L+ ++F D+S + + YL +++ G L YLY L+ ++ G
Sbjct: 551 HLISSMIFADKSLAHVHVAYL*YLSN-LTACHK*V*GVAALVYLYDHLSYVSLDDNKQCG 375
Query: 169 GSVTLLTTWFLAHFP--GFFSVDLNTDYLENYPVAARWKLPKGHGEGITYWSLLDRIQLD 226
+TLL H P G+ + + DY + P+ RW G + ++ I ++
Sbjct: 374 DYMTLLMA*VFEHLPSVGYSNPE---DYSDGDPLNTRWNPLGGTRHALLVKERMNNIHMN 204
Query: 227 DVCYKPYKEHREIQDFEEVFWYSGWIMCGVRRVYRHLPKRVLRQYGYVQTIP 278
PY+ ++ ++ F E G+ C + + +L KRV RQ+ +VQTIP
Sbjct: 203 VFI*CPYE*NKLVRPFVEQTL*FGYFKCRID-MRSYLLKRVRRQFCHVQTIP 51
>TC232778 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16, partial (5%)
Length = 746
Score = 48.1 bits (113), Expect = 5e-06
Identities = 44/153 (28%), Positives = 67/153 (43%)
Frame = -1
Query: 4 GEMTVTLDDVACLTHLPIEGRMLDHGKKMPKHEGAAQLMTYLGVSQHEAEKICNQKYGGY 63
GE T+TL DV L L +G L + + +L LGV E E G
Sbjct: 422 GEATITLQDVLILLGLRTDGAPLIGSTNLDWADLCEEL---LGVRPQEGEI-----EGSV 267
Query: 64 ISYPRLRDFYTSYLGRANVLAGTEDPEELEELARVRTYCVRCYLLYLVGCLLFGDRSNKR 123
+ L Y N+ D +E+L R +R ++L +G ++F ++S+ R
Sbjct: 266 VKLSWL----AHYFSHINI-----DEGNVEQLQRF----IRAWILRFIGGVIFVNKSSSR 126
Query: 124 IELIYLTTMADGYAGMRNYSWGGMTLAYLYGEL 156
+ L YL + D + Y+WG LAYLY E+
Sbjct: 125 VSLRYLQFLRD-FE*CSTYAWGPAMLAYLYREM 30
>TC234144
Length = 468
Score = 35.4 bits (80), Expect = 0.032
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = +3
Query: 220 LDRIQLDDVCYKPYKEHREIQDFEEVFWYSGWIMCG 255
LD + D+ + PY HRE + F ++ +SG+I CG
Sbjct: 15 LDML*TKDILWMPYTVHREHRPFHDISLFSGYIRCG 122
>BG881451 weakly similar to PIR|D96521|D96 protein F21D18.16 [imported] -
Arabidopsis thaliana, partial (1%)
Length = 421
Score = 33.5 bits (75), Expect = 0.12
Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 5/70 (7%)
Frame = +3
Query: 214 ITYWSLLDRIQLDDVCYKPYK--EHREIQDFEE---VFWYSGWIMCGVRRVYRHLPKRVL 268
+ Y LD ++ DV + PY+ + I ++ + + S ++ + RHLP R L
Sbjct: 6 VFYRKSLDSLKPCDVEWLPYRNMDSMVIPEYIKSTLILGRSKTMLICFDKAERHLPNRCL 185
Query: 269 RQYGYVQTIP 278
RQYG +Q+IP
Sbjct: 186 RQYGMLQSIP 215
>BF071225 weakly similar to PIR|D96521|D96 protein F21D18.16 [imported] -
Arabidopsis thaliana, partial (4%)
Length = 359
Score = 31.2 bits (69), Expect = 0.61
Identities = 17/43 (39%), Positives = 23/43 (52%)
Frame = +2
Query: 245 VFWYSGWIMCGVRRVYRHLPKRVLRQYGYVQTIPRHPTDVRDL 287
V W++ + V H P RVLRQYG Q IP P+ ++L
Sbjct: 152 VVWFAVVPLICFHVVEWHQPDRVLRQYGLQQPIPGCPSQPQNL 280
>BI470376 weakly similar to GP|14090338|dbj P0638D12.5 {Oryza sativa
(japonica cultivar-group)}, partial (5%)
Length = 429
Score = 29.6 bits (65), Expect = 1.8
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = -1
Query: 4 GEMTVTLDDVACLTHLPIEGRML 26
GE T+TL DV+ L +P++GR L
Sbjct: 273 GEATITLQDVSVLLGIPVDGRPL 205
>TC215161 similar to UP|Q6NQ77 (Q6NQ77) At4g05190, partial (19%)
Length = 692
Score = 28.9 bits (63), Expect = 3.0
Identities = 16/70 (22%), Positives = 35/70 (49%)
Frame = -3
Query: 19 LPIEGRMLDHGKKMPKHEGAAQLMTYLGVSQHEAEKICNQKYGGYISYPRLRDFYTSYLG 78
L ++ + +H P EG+ + T + V ++ Q Y+S+ L ++S+L
Sbjct: 420 LTLDANLSEHSDS-PTEEGSGDMFTNISVLEYPPR----QG*SRYVSFEFLNGTWSSFLA 256
Query: 79 RANVLAGTED 88
+AN+ + ++D
Sbjct: 255 KANITSLSDD 226
>TC220808 weakly similar to UP|Q9SK32 (Q9SK32) Expressed protein, partial
(9%)
Length = 869
Score = 28.9 bits (63), Expect = 3.0
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1
Query: 261 RHLPKRVLRQYGYVQTIPR 279
RHLP R LRQ+ QTIP+
Sbjct: 58 RHLPDRCLRQFAMHQTIPK 114
>BE803668 weakly similar to GP|1181599|dbj| subunit of photosystem I {Cucumis
sativus}, partial (18%)
Length = 400
Score = 28.1 bits (61), Expect = 5.1
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +1
Query: 279 RHPTDVRDLPTAFHCADVRRLPHS 302
R P +R L +HC D++ LPHS
Sbjct: 205 RSPC*LRSLSGGWHCGDLQSLPHS 276
>AW279090
Length = 335
Score = 27.7 bits (60), Expect = 6.7
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 3/33 (9%)
Frame = -1
Query: 274 VQTIPRHPTDVRDLPTAFH---CADVRRLPHSH 303
++TIPR T++RDLP + + A+ + HSH
Sbjct: 215 IRTIPREATNIRDLPISQYLLASAEAVLINHSH 117
>AI965377 similar to GP|21592780|gb| nucleic acid binding protein-like
{Arabidopsis thaliana}, partial (25%)
Length = 380
Score = 27.3 bits (59), Expect = 8.8
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +2
Query: 99 RTYCVRCYLLYLVGCLLF 116
+ YC RCY+ Y++ C F
Sbjct: 302 QVYCFRCYIRYIMSCCRF 355
>TC206438 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (93%)
Length = 1133
Score = 27.3 bits (59), Expect = 8.8
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +2
Query: 99 RTYCVRCYLLYLVGCLLF 116
+ YC RCY+ Y++ C F
Sbjct: 998 QVYCFRCYIRYIMSCCRF 1051
>BE209913 similar to GP|10946375|gb ribulose-1 5-bisphosphate carboxylase
small subunit rbcS1 {Glycine max}, partial (68%)
Length = 367
Score = 27.3 bits (59), Expect = 8.8
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +2
Query: 209 GHGEGITYWSLLDRIQLDDVCYKPYKEHREIQDFEEVFWYSGWIMC 254
G + + W + + D +CY P + ++ + EE GWI C
Sbjct: 149 GRVQCMQVWPPGGKKKFDTLCYLPDLDDAQLANEEEYLLRKGWIPC 286
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.142 0.463
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,504,604
Number of Sequences: 63676
Number of extensions: 261468
Number of successful extensions: 1314
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 1303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1308
length of query: 304
length of database: 12,639,632
effective HSP length: 97
effective length of query: 207
effective length of database: 6,463,060
effective search space: 1337853420
effective search space used: 1337853420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 59 (27.3 bits)
Medicago: description of AC139854.8