BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139854.4 + phase: 0
(299 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205467 410 e-115
TC205468 weakly similar to GB|AAC35522.1|3600034|T12H20 T12H20.2... 263 9e-71
TC205469 similar to UP|Q6UA19 (Q6UA19) Fiber protein Fb5 (Fragme... 188 3e-48
TC205464 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g5... 174 3e-44
AW201799 89 2e-18
TC205465 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g5... 85 3e-17
TC205466 similar to UP|Q6UA19 (Q6UA19) Fiber protein Fb5 (Fragme... 78 4e-15
BQ081032 63 2e-10
TC223796 homologue to GB|AAN46775.1|24111303|BT001021 At2g42880/... 32 0.27
TC206609 similar to GB|AAQ22645.1|33589758|BT010176 At5g24810/F6... 29 2.3
TC230502 weakly similar to GB|AAN38687.1|23507767|BT000850 At3g0... 28 3.8
TC234403 similar to GB|AAQ89644.1|37202058|BT010622 At4g17310 {A... 28 3.8
BI470595 28 3.8
TC214101 similar to GB|AAP31933.1|30387535|BT006589 At4g26850 {A... 28 5.0
TC214291 similar to UP|Q8LKQ7 (Q8LKQ7) VTC2, partial (15%) 28 5.0
CB063697 28 5.0
BE803782 28 6.6
TC220866 similar to UP|Q7VB79 (Q7VB79) Predicted protein family ... 28 6.6
TC206918 weakly similar to UP|Q8GTM7 (Q8GTM7) Putataive glucan 1... 28 6.6
AW423706 28 6.6
>TC205467
Length = 1504
Score = 410 bits (1054), Expect = e-115
Identities = 200/301 (66%), Positives = 231/301 (76%), Gaps = 2/301 (0%)
Frame = +1
Query: 1 MYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLF 60
MYFPK G+CY+FYDPVQRKT++ ELPEL+G RVCYTKDGWLLL R R+ F
Sbjct: 178 MYFPKFGDCYEFYDPVQRKTHTFELPELNGSRVCYTKDGWLLLYRPRTHRV------FFF 339
Query: 61 NPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTVY 120
NPFTR+LI LP+F+ TYQI AFSCAPTS GCV+ + V ++VAISTCYPG EWTTV
Sbjct: 340 NPFTRELIKLPRFEMTYQIVAFSCAPTSPGCVLFTVKHVSPTVVAISTCYPGATEWTTVN 519
Query: 121 YDAELS--CSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVFKVPPPKCLAESSTAKN 178
Y L S+ +KLVF NGLFYCLS GWLGVFDP+E TW+V VPPPKC E+ AKN
Sbjct: 520 YQNRLPFVSSIWNKLVFCNGLFYCLSLTGWLGVFDPVECTWSVLAVPPPKC-PENFFAKN 696
Query: 179 WSKGKFMIEHKGNIFVVHICCGEDPIIFKLDLTLMEWKEVRSLNGVTLFASFLSSHSRTY 238
W KGKFM EH+G+I V++ CC E+PIIFKLD TLM+W+E+ +L+GVTLFASFLSSHSRT
Sbjct: 697 WWKGKFMAEHEGDILVIYTCCSENPIIFKLDQTLMKWEEMTTLDGVTLFASFLSSHSRTD 876
Query: 239 ATGIMRNSVYFPKVRFYGKRCISFSLDDRRYYPSEQCRDKVEPNTFENFWIEPPKDFTGW 298
GIMRNSVYF KVRFYGKRCISFSL D RYYP +QC D E + FEN WIEP KDF+
Sbjct: 877 LIGIMRNSVYFSKVRFYGKRCISFSLGDYRYYPRKQCHDWGEHDPFENIWIEPSKDFSAC 1056
Query: 299 M 299
M
Sbjct: 1057M 1059
>TC205468 weakly similar to GB|AAC35522.1|3600034|T12H20 T12H20.2
{Arabidopsis thaliana;} , partial (21%)
Length = 867
Score = 263 bits (671), Expect = 9e-71
Identities = 128/193 (66%), Positives = 148/193 (76%), Gaps = 2/193 (1%)
Frame = +2
Query: 108 TCYPGEKEWTTVYYDAELS--CSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVFKVP 165
TCYPG EWTT+ Y L S+ +KLVF NGLFYCLS GWLGVF+ ERTW+V VP
Sbjct: 8 TCYPGATEWTTLSYQNRLPFVSSIWNKLVFCNGLFYCLSLTGWLGVFNSSERTWSVLSVP 187
Query: 166 PPKCLAESSTAKNWSKGKFMIEHKGNIFVVHICCGEDPIIFKLDLTLMEWKEVRSLNGVT 225
PPKC E+ AKNW KGKFM EH+G+I V++ C E+PIIFKLD LMEW+E+ +L+GVT
Sbjct: 188 PPKC-PENFFAKNWWKGKFMTEHEGDIIVIYTCSSENPIIFKLDQMLMEWEEMTTLDGVT 364
Query: 226 LFASFLSSHSRTYATGIMRNSVYFPKVRFYGKRCISFSLDDRRYYPSEQCRDKVEPNTFE 285
LFASFLSSH+R GIMRNSVYF KVRFYGKRCISFSLDD RYYP +Q D E + FE
Sbjct: 365 LFASFLSSHARIDLPGIMRNSVYFSKVRFYGKRCISFSLDDCRYYPRKQWHDWGEQDPFE 544
Query: 286 NFWIEPPKDFTGW 298
N WIEPPKDF+G+
Sbjct: 545 NIWIEPPKDFSGF 583
>TC205469 similar to UP|Q6UA19 (Q6UA19) Fiber protein Fb5 (Fragment), partial
(92%)
Length = 694
Score = 188 bits (477), Expect = 3e-48
Identities = 90/132 (68%), Positives = 107/132 (80%)
Frame = +1
Query: 167 PKCLAESSTAKNWSKGKFMIEHKGNIFVVHICCGEDPIIFKLDLTLMEWKEVRSLNGVTL 226
PKC E+ AKNW KGKFM EH+G+I V++ C E+PIIFKLDLTL+EW+E+ +L+GVTL
Sbjct: 1 PKC-PENFFAKNWWKGKFMTEHEGDIIVIYTCSNENPIIFKLDLTLLEWEEMTTLDGVTL 177
Query: 227 FASFLSSHSRTYATGIMRNSVYFPKVRFYGKRCISFSLDDRRYYPSEQCRDKVEPNTFEN 286
FASFLSSH+RT GIMRNSV+F KVRFYGKRCISFSLD RYYP +Q D E + FEN
Sbjct: 178 FASFLSSHARTDLHGIMRNSVFFSKVRFYGKRCISFSLDGYRYYPRKQWHDWGEQDPFEN 357
Query: 287 FWIEPPKDFTGW 298
WIEPPKDF+G+
Sbjct: 358 IWIEPPKDFSGF 393
>TC205464 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g57790
{Arabidopsis thaliana;} , partial (17%)
Length = 756
Score = 174 bits (442), Expect = 3e-44
Identities = 88/151 (58%), Positives = 105/151 (69%), Gaps = 2/151 (1%)
Frame = +1
Query: 1 MYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLF 60
MYFPK G+ Y+FYDP RKTYS+ELPEL G RVCYTKDGWLLL R R+ F
Sbjct: 322 MYFPKFGDWYEFYDPAHRKTYSIELPELRGSRVCYTKDGWLLLYRPRTHRV------FFF 483
Query: 61 NPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTVY 120
NPFT ++I LP+F+ +YQI AFSCAPTS CV++ + V + VAISTCYPG EWTT+
Sbjct: 484 NPFTMEIIKLPRFEMSYQIVAFSCAPTSPDCVLVTGKHVSPTGVAISTCYPGATEWTTLS 663
Query: 121 YDAELS--CSMCDKLVFSNGLFYCLSDRGWL 149
Y L+ S+ +KL F NGLFY LS GWL
Sbjct: 664 YQNRLAFVSSIWNKLGFGNGLFYWLSLTGWL 756
>AW201799
Length = 292
Score = 89.0 bits (219), Expect = 2e-18
Identities = 43/84 (51%), Positives = 54/84 (64%), Gaps = 2/84 (2%)
Frame = +2
Query: 60 FNPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTV 119
FNPFT ++I LP F+ TYQI AFSCA TS CV V ++ A+STCYP +WTT+
Sbjct: 41 FNPFTMEIINLPTFEMTYQIVAFSCASTSPDCVPSTAHHVSPTVAALSTCYPAATQWTTL 220
Query: 120 YYD--AELSCSMCDKLVFSNGLFY 141
Y + S+C+KLV NGLFY
Sbjct: 221 SYQTRSPFVSSLCNKLVS*NGLFY 292
>TC205465 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g57790
{Arabidopsis thaliana;} , partial (15%)
Length = 490
Score = 85.1 bits (209), Expect = 3e-17
Identities = 41/63 (65%), Positives = 44/63 (69%)
Frame = +3
Query: 1 MYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLF 60
MYFPK G+ Y+FYDPV RKTYS+ELPEL G RVCYTKDGWLLL R H F
Sbjct: 318 MYFPKFGDWYEFYDPVHRKTYSIELPELSGSRVCYTKDGWLLLYR------PRTHRVFFF 479
Query: 61 NPF 63
NPF
Sbjct: 480 NPF 488
>TC205466 similar to UP|Q6UA19 (Q6UA19) Fiber protein Fb5 (Fragment), partial
(92%)
Length = 754
Score = 78.2 bits (191), Expect = 4e-15
Identities = 34/47 (72%), Positives = 37/47 (78%)
Frame = +3
Query: 253 RFYGKRCISFSLDDRRYYPSEQCRDKVEPNTFENFWIEPPKDFTGWM 299
RFYGKRCISFSLDD RYYP +QC D E + FEN WIEPPKDF+ M
Sbjct: 3 RFYGKRCISFSLDDYRYYPRKQCHDWGEQDPFENIWIEPPKDFSACM 143
>BQ081032
Length = 182
Score = 62.8 bits (151), Expect = 2e-10
Identities = 31/58 (53%), Positives = 36/58 (61%), Gaps = 2/58 (3%)
Frame = +1
Query: 114 KEWTTVYYDAELS--CSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVFKVPPPKC 169
KEWT++ Y L S+ +KLVF NGLFY L GWLGVF+ E TW VP PKC
Sbjct: 1 KEWTSLSYQNRLXFVSSIWNKLVFCNGLFYWLXFTGWLGVFNLSEATWAGLSVPSPKC 174
>TC223796 homologue to GB|AAN46775.1|24111303|BT001021 At2g42880/F7D19.12
{Arabidopsis thaliana;} , partial (24%)
Length = 439
Score = 32.3 bits (72), Expect = 0.27
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +2
Query: 40 WLLLNRQDWRRLDGNHIFSLFNPFTRDL 67
WL+L +DW++L GNH+ SL + +L
Sbjct: 323 WLILTSRDWQKLRGNHLASLSRKWNLNL 406
>TC206609 similar to GB|AAQ22645.1|33589758|BT010176 At5g24810/F6A4.20
{Arabidopsis thaliana;} , partial (21%)
Length = 1157
Score = 29.3 bits (64), Expect = 2.3
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 150 GVFDPLERTWTVFKVPPPKCLAESSTA 176
G+F+ +WT+F V PPK + S TA
Sbjct: 908 GIFNSEHTSWTIFPVTPPKDILFSVTA 828
>TC230502 weakly similar to GB|AAN38687.1|23507767|BT000850
At3g07720/F17A17_6 {Arabidopsis thaliana;} , partial
(43%)
Length = 633
Score = 28.5 bits (62), Expect = 3.8
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
Frame = +1
Query: 152 FDPLERTWTVFKVPPPKCLAESSTAKNWSKGKFMIEHKGNIFVV---HICCGE-DPIIFK 207
FDP ++TW + K A S ++S GK +I + G I H+ G+ ++
Sbjct: 73 FDPAQKTWAQVETSGQKPTARSVFC-SFSDGKHIIVYGGEIDPSDQGHMGAGQFSGEVYA 249
Query: 208 LDLTLMEWKEV 218
LD+ + WK +
Sbjct: 250 LDMETLAWKRL 282
>TC234403 similar to GB|AAQ89644.1|37202058|BT010622 At4g17310 {Arabidopsis
thaliana;} , partial (58%)
Length = 434
Score = 28.5 bits (62), Expect = 3.8
Identities = 16/38 (42%), Positives = 19/38 (49%), Gaps = 6/38 (15%)
Frame = -2
Query: 149 LGVFDPLERTWTVFKVPPPKCLAESST------AKNWS 180
LG F T F PPP+C A +ST AK+WS
Sbjct: 262 LGSFWKSRSDTTSFSPPPPRCAAATSTARISAPAKSWS 149
>BI470595
Length = 413
Score = 28.5 bits (62), Expect = 3.8
Identities = 16/38 (42%), Positives = 19/38 (49%), Gaps = 6/38 (15%)
Frame = -3
Query: 149 LGVFDPLERTWTVFKVPPPKCLAESST------AKNWS 180
LG F T F PPP+C A +ST AK+WS
Sbjct: 303 LGSFWKSRSDTTSFSPPPPRCAAATSTARISAPAKSWS 190
>TC214101 similar to GB|AAP31933.1|30387535|BT006589 At4g26850 {Arabidopsis
thaliana;} , partial (81%)
Length = 2092
Score = 28.1 bits (61), Expect = 5.0
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +2
Query: 190 GNIFVVHICCGEDPIIFKLDLTLMEWKE 217
G +H C GE P+ F L L EW++
Sbjct: 596 GKDLALHACAGEPPLAFLDSLLLGEWED 679
>TC214291 similar to UP|Q8LKQ7 (Q8LKQ7) VTC2, partial (15%)
Length = 874
Score = 28.1 bits (61), Expect = 5.0
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +2
Query: 190 GNIFVVHICCGEDPIIFKLDLTLMEWKE 217
G +H C GE P+ F L L EW++
Sbjct: 701 GKDLALHACAGEPPLTFLDSLLLGEWED 784
>CB063697
Length = 356
Score = 28.1 bits (61), Expect = 5.0
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +3
Query: 190 GNIFVVHICCGEDPIIFKLDLTLMEWKE 217
G +H C GE P+ F L L EW++
Sbjct: 189 GKDLALHACAGEPPLAFLDSLLLGEWED 272
>BE803782
Length = 416
Score = 27.7 bits (60), Expect = 6.6
Identities = 16/38 (42%), Positives = 19/38 (49%), Gaps = 6/38 (15%)
Frame = -1
Query: 149 LGVFDPLERTWTVFKVPPPKCLAESSTA------KNWS 180
LG F T F PPP+C A +STA K+WS
Sbjct: 161 LGSFWKSRSGATSFSPPPPRCAAATSTARISAPPKSWS 48
>TC220866 similar to UP|Q7VB79 (Q7VB79) Predicted protein family PM-3, partial
(9%)
Length = 1161
Score = 27.7 bits (60), Expect = 6.6
Identities = 15/46 (32%), Positives = 24/46 (51%), Gaps = 5/46 (10%)
Frame = -2
Query: 165 PPPK--CLAESSTAKNWSKGKFMIEHKGNIFV---VHICCGEDPII 205
PPP CL T+K +G + KGN+++ + +C DP+I
Sbjct: 1160 PPPSLSCLLLKETSKKNDEGIINQQMKGNVYIMSPILLCFNLDPLI 1023
>TC206918 weakly similar to UP|Q8GTM7 (Q8GTM7) Putataive glucan
1,3-beta-glucosidase, partial (40%)
Length = 1544
Score = 27.7 bits (60), Expect = 6.6
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -3
Query: 23 LELPELDGCRVCYTKDGWLLLN 44
LE P L GC C+ KDGW++++
Sbjct: 432 LEFP-L*GCNDCHLKDGWVIIS 370
>AW423706
Length = 323
Score = 27.7 bits (60), Expect = 6.6
Identities = 16/38 (42%), Positives = 19/38 (49%), Gaps = 6/38 (15%)
Frame = -1
Query: 149 LGVFDPLERTWTVFKVPPPKCLAESSTA------KNWS 180
LG F T F PPP+C A +STA K+WS
Sbjct: 155 LGSFWKSRSGATSFSPPPPRCAAATSTARISAPPKSWS 42
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.142 0.476
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,798,582
Number of Sequences: 63676
Number of extensions: 326711
Number of successful extensions: 1748
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1740
length of query: 299
length of database: 12,639,632
effective HSP length: 97
effective length of query: 202
effective length of database: 6,463,060
effective search space: 1305538120
effective search space used: 1305538120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC139854.4