Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC139745.7 + phase: 0 
         (240 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC218434 similar to UP|PIF3_ARATH (O80536) Phytochrome-interacti...   151  2e-37
TC228741 similar to UP|PIF3_ARATH (O80536) Phytochrome-interacti...   114  3e-26
TC210945 similar to GB|BAC56979.1|28372351|AB103113 PIF3 like ba...    91  5e-19
TC208663 similar to UP|PIF4_ARATH (Q8W2F3) Phytochrome-interacti...    90  8e-19
TC218350 similar to GB|BAC56979.1|28372351|AB103113 PIF3 like ba...    90  8e-19
AW782148 homologue to GP|11245494|gb| SPATULA {Arabidopsis thali...    87  5e-18
TC207452 similar to UP|Q9FUA4 (Q9FUA4) SPATULA, partial (31%)          86  2e-17
BG352463 similar to GP|11245494|gb| SPATULA {Arabidopsis thalian...    84  6e-17
TC231105                                                               80  1e-15
TC217804                                                               77  7e-15
TC205173 homologue to UP|Q6K8Y4 (Q6K8Y4) Basic helix-loop-helix ...    69  2e-12
TC205174 homologue to UP|Q6K8Y4 (Q6K8Y4) Basic helix-loop-helix ...    69  2e-12
TC207545 homologue to GB|AAN28890.1|23506061|AY143951 At4g02590/...    66  1e-11
TC229602 homologue to GB|AAN28890.1|23506061|AY143951 At4g02590/...    66  1e-11
TC219879 homologue to UP|Q6K8Y4 (Q6K8Y4) Basic helix-loop-helix ...    65  2e-11
TC229276 weakly similar to UP|SRE2_HUMAN (Q12772) Sterol regulat...    64  5e-11
TC215736 similar to GB|AAO63377.1|28950907|BT005313 At4g34530 {A...    62  3e-10
TC218502                                                               61  5e-10
TC231689 weakly similar to UP|Q38736 (Q38736) DEL, partial (22%)       61  5e-10
TC205597 similar to GB|AAO63377.1|28950907|BT005313 At4g34530 {A...    60  7e-10

>TC218434 similar to UP|PIF3_ARATH (O80536) Phytochrome-interacting factor 3
           (Phytochrome-associated protein 3) (Basic
           helix-loop-helix protein 8) (bHLH8) (AtbHLH008), partial
           (11%)
          Length = 832

 Score =  151 bits (382), Expect = 2e-37
 Identities = 80/119 (67%), Positives = 95/119 (79%)
 Frame = +2

Query: 112 ASNDLNFGVRKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSER 171
           ASND N G RK HEDTDDS YLSDND E ++ + ++   REGNRVKRS RN +VHNLSE+
Sbjct: 2   ASNDPNLGFRK-HEDTDDSTYLSDNDGEPEDMVKQD---REGNRVKRS-RNPEVHNLSEK 166

Query: 172 KRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQVKFYVLQFSVTLFKL 230
           KRR+KIN+K+R LK+LIPNCNK+DKASMLDDAIDYLKTLKLQLQ  F ++     L+ L
Sbjct: 167 KRREKINKKMRTLKDLIPNCNKVDKASMLDDAIDYLKTLKLQLQANFQIMSMGSGLWPL 343


>TC228741 similar to UP|PIF3_ARATH (O80536) Phytochrome-interacting factor 3
           (Phytochrome-associated protein 3) (Basic
           helix-loop-helix protein 8) (bHLH8) (AtbHLH008), partial
           (25%)
          Length = 763

 Score =  114 bits (286), Expect = 3e-26
 Identities = 65/116 (56%), Positives = 84/116 (72%), Gaps = 4/116 (3%)
 Frame = +3

Query: 105 SSVCSL----EASNDLNFGVRKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSY 160
           SSVCS     + S + N  +++  +DTDDS   S++ EE      K    + G   KRS 
Sbjct: 117 SSVCSGNGTDQGSEEPNQNLKRKTKDTDDSECHSEDVEEESAGAKKTAGGQGGAGSKRS- 293

Query: 161 RNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           R A+VHNLSERKRRD+INEK+RAL+ELIPNCNK+DKASMLD+AI+YLKTL+LQ+Q+
Sbjct: 294 RAAEVHNLSERKRRDRINEKMRALQELIPNCNKVDKASMLDEAIEYLKTLQLQVQI 461


>TC210945 similar to GB|BAC56979.1|28372351|AB103113 PIF3 like basic Helix
           Loop Helix protein {Arabidopsis thaliana;} , partial
           (19%)
          Length = 432

 Score = 90.9 bits (224), Expect = 5e-19
 Identities = 49/82 (59%), Positives = 63/82 (76%)
 Frame = +2

Query: 135 DNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKM 194
           D D E+ E    +K V      KRS+  A+VHNLSER RRD+INEK++AL+ELIP CNK 
Sbjct: 32  DVDFESPE---AKKQVHGSTSTKRSHA-AEVHNLSERSRRDRINEKMKALQELIPRCNKS 199

Query: 195 DKASMLDDAIDYLKTLKLQLQV 216
           DKASMLD+AI+YLK+L+LQ+Q+
Sbjct: 200 DKASMLDEAIEYLKSLQLQVQM 265


>TC208663 similar to UP|PIF4_ARATH (Q8W2F3) Phytochrome-interacting factor 4
           (Basic helix-loop-helix protein 9) (bHLH9) (AtbHLH009)
           (Short under red-light 2), partial (19%)
          Length = 1102

 Score = 90.1 bits (222), Expect = 8e-19
 Identities = 54/128 (42%), Positives = 82/128 (63%)
 Frame = +1

Query: 108 CSLEASNDLNFGVRKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHN 167
           CSL   N    G ++   D ++S   S+ D E +  +  +   R G+   R  R A+VHN
Sbjct: 118 CSLSTRNQ---GQKRKGIDVEESEEQSE-DTELKSALGNKSSQRTGSA--RRNRAAEVHN 279

Query: 168 LSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQVKFYVLQFSVTL 227
           LSER+RRD+INEK++AL++LIP+ +K DKASML++AI+YLK+L+LQLQ+ +     +  +
Sbjct: 280 LSERRRRDRINEKMKALQQLIPHSSKTDKASMLEEAIEYLKSLQLQLQLMWMGSGMAPIM 459

Query: 228 FKLRFHYI 235
           F    HY+
Sbjct: 460 FPGIQHYM 483


>TC218350 similar to GB|BAC56979.1|28372351|AB103113 PIF3 like basic Helix
           Loop Helix protein {Arabidopsis thaliana;} , partial
           (25%)
          Length = 1060

 Score = 90.1 bits (222), Expect = 8e-19
 Identities = 46/70 (65%), Positives = 58/70 (82%)
 Frame = +1

Query: 147 EKPVREGNRVKRSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDY 206
           +K V      KRS R A+VHNLSER+RRD+INEK++AL+ELIP CNK DKASMLD+AI Y
Sbjct: 31  KKQVCGSTSTKRS-RAAEVHNLSERRRRDRINEKMKALQELIPRCNKSDKASMLDEAISY 207

Query: 207 LKTLKLQLQV 216
           LK+L+LQ+Q+
Sbjct: 208 LKSLQLQVQM 237


>AW782148 homologue to GP|11245494|gb| SPATULA {Arabidopsis thaliana},
           partial (17%)
          Length = 381

 Score = 87.4 bits (215), Expect = 5e-18
 Identities = 50/105 (47%), Positives = 67/105 (63%)
 Frame = +1

Query: 112 ASNDLNFGVRKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSER 171
           A+N  +     S  + DD    S+   E     V  K     +  KRS R A+VHNLSE+
Sbjct: 70  AANVSSSSAGVSENENDDYDCESEEGVEALAEEVPTKAASSRSSSKRS-RAAEVHNLSEK 246

Query: 172 KRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           +RR +INEK++AL+ LIPN NK DKASMLD+AI+YLK L+LQ+Q+
Sbjct: 247 RRRSRINEKMKALQNLIPNSNKTDKASMLDEAIEYLKQLQLQVQM 381


>TC207452 similar to UP|Q9FUA4 (Q9FUA4) SPATULA, partial (31%)
          Length = 1207

 Score = 85.5 bits (210), Expect = 2e-17
 Identities = 49/105 (46%), Positives = 67/105 (63%)
 Frame = +2

Query: 112 ASNDLNFGVRKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSER 171
           A+N  +     S  + DD    S+  +E     V  K     +  KRS R A+VHN SE+
Sbjct: 5   AANVSSSSAGVSENENDDYDCESEEGDEAPAEEVPTKAASARSSSKRS-RAAEVHN*SEK 181

Query: 172 KRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           +RR +INEK++AL+ LIPN NK DKASMLD+AI+YLK L+LQ+Q+
Sbjct: 182 RRRGRINEKMKALQNLIPNSNKTDKASMLDEAIEYLKQLQLQVQM 316


>BG352463 similar to GP|11245494|gb| SPATULA {Arabidopsis thaliana}, partial
           (19%)
          Length = 471

 Score = 84.0 bits (206), Expect = 6e-17
 Identities = 44/86 (51%), Positives = 58/86 (67%)
 Frame = +3

Query: 148 KPVREGNRVKRSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYL 207
           KPV       +  R A+ HNLSE++RR +INEK++AL+ LIPN NK DKASMLD+AI+YL
Sbjct: 9   KPVPPPRSSSKRSRAAEFHNLSEKRRRSRINEKMKALQNLIPNSNKTDKASMLDEAIEYL 188

Query: 208 KTLKLQLQVKFYVLQFSVTLFKLRFH 233
           K L+LQ+Q    +LQ  +    L  H
Sbjct: 189 KQLQLQVQYL*LILQMLMMRNGLSLH 266


>TC231105 
          Length = 449

 Score = 79.7 bits (195), Expect = 1e-15
 Identities = 45/96 (46%), Positives = 63/96 (64%), Gaps = 1/96 (1%)
 Frame = +2

Query: 122 KSHEDTDDS-PYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSERKRRDKINEK 180
           + H+    S P   D DEE ++    +  V      KRS R A +HN SERKRRDKIN++
Sbjct: 65  EEHDSVSHSKPMGEDQDEEKKKRANGKSSVS----TKRS-RAAAIHNQSERKRRDKINQR 229

Query: 181 IRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           ++ L++L+PN +K DKASMLD+ I+YLK L+ QLQ+
Sbjct: 230 MKTLQKLVPNSSKSDKASMLDEVIEYLKQLQAQLQM 337


>TC217804 
          Length = 1578

 Score = 77.0 bits (188), Expect = 7e-15
 Identities = 38/108 (35%), Positives = 64/108 (59%)
 Frame = +1

Query: 123 SHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSERKRRDKINEKIR 182
           +++D D   +     E   E+    K  R     KR   N+ VH  SER+RRDKIN++++
Sbjct: 694 TNDDRDSISHRISQGEVPDEDYKATKVDRSSGSNKRIKANSVVHKQSERRRRDKINQRMK 873

Query: 183 ALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQVKFYVLQFSVTLFKL 230
            L++L+PN +K DKASMLD+ I Y+K L+ Q+Q+  ++  ++  +  +
Sbjct: 874 ELQKLVPNSSKTDKASMLDEVIQYMKQLQAQVQMMNWMKMYTTMMLPI 1017


>TC205173 homologue to UP|Q6K8Y4 (Q6K8Y4) Basic helix-loop-helix (BHLH)-like,
           partial (29%)
          Length = 1490

 Score = 68.9 bits (167), Expect = 2e-12
 Identities = 34/69 (49%), Positives = 52/69 (75%), Gaps = 1/69 (1%)
 Frame = +2

Query: 149 PVREGNRVK-RSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYL 207
           P +   RV+ R  +    H+++ER RR++I E+++AL+EL+PN NK DKASMLD+ IDY+
Sbjct: 458 PAQPRQRVRARRGQATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIIDYV 637

Query: 208 KTLKLQLQV 216
           K L+LQ++V
Sbjct: 638 KFLQLQVKV 664


>TC205174 homologue to UP|Q6K8Y4 (Q6K8Y4) Basic helix-loop-helix (BHLH)-like,
           partial (29%)
          Length = 845

 Score = 68.9 bits (167), Expect = 2e-12
 Identities = 34/69 (49%), Positives = 52/69 (75%), Gaps = 1/69 (1%)
 Frame = +1

Query: 149 PVREGNRVK-RSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYL 207
           P +   RV+ R  +    H+++ER RR++I E+++AL+EL+PN NK DKASMLD+ IDY+
Sbjct: 112 PAQPRQRVRARRGQATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIIDYV 291

Query: 208 KTLKLQLQV 216
           K L+LQ++V
Sbjct: 292 KFLQLQVKV 318


>TC207545 homologue to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P11_13
           {Arabidopsis thaliana;} , partial (53%)
          Length = 1392

 Score = 66.2 bits (160), Expect = 1e-11
 Identities = 28/51 (54%), Positives = 45/51 (87%)
 Frame = +1

Query: 166 HNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           H+++ER RR++I E+IRAL+EL+P+ NK D+A+MLD+ +DY+K L+LQ++V
Sbjct: 307 HSIAERLRRERIAERIRALQELVPSVNKTDRAAMLDEIVDYVKFLRLQVKV 459


>TC229602 homologue to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P11_13
           {Arabidopsis thaliana;} , partial (54%)
          Length = 1052

 Score = 66.2 bits (160), Expect = 1e-11
 Identities = 28/51 (54%), Positives = 45/51 (87%)
 Frame = +1

Query: 166 HNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           H+++ER RR++I E+IRAL+EL+P+ NK D+A+MLD+ +DY+K L+LQ++V
Sbjct: 181 HSIAERLRRERIAERIRALQELVPSVNKTDRAAMLDEIVDYVKFLRLQVKV 333


>TC219879 homologue to UP|Q6K8Y4 (Q6K8Y4) Basic helix-loop-helix (BHLH)-like,
           partial (27%)
          Length = 1114

 Score = 65.5 bits (158), Expect = 2e-11
 Identities = 29/51 (56%), Positives = 44/51 (85%)
 Frame = +3

Query: 166 HNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           H+++ER RR++I E+++AL+EL+ N NK DKASMLD+ IDY+K L+LQ++V
Sbjct: 273 HSIAERLRRERIAERMKALQELVTNANKTDKASMLDEIIDYVKFLQLQVKV 425


>TC229276 weakly similar to UP|SRE2_HUMAN (Q12772) Sterol regulatory element
           binding protein-2 (SREBP-2) (Sterol regulatory
           element-binding transcription factor 2), partial (3%)
          Length = 1069

 Score = 64.3 bits (155), Expect = 5e-11
 Identities = 42/111 (37%), Positives = 63/111 (55%), Gaps = 1/111 (0%)
 Frame = +2

Query: 102 QKPSS-VCSLEASNDLNFGVRKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSY 160
           QKP S + S E S  +    +K+ +  D      ++ +ETQE     KP++ G R  ++ 
Sbjct: 245 QKPKSCILSFEDSTLVPINPKKTCQIYDHG----EHSKETQEKPHNRKPLKRGRRFSQTL 412

Query: 161 RNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLK 211
                H L+ERKRR+ I+    AL  LIP+  KMDKAS+L +AI+Y+K L+
Sbjct: 413 D----HILAERKRRENISRMFIALSALIPDLKKMDKASVLSNAIEYVKYLQ 553


>TC215736 similar to GB|AAO63377.1|28950907|BT005313 At4g34530 {Arabidopsis
           thaliana;} , partial (28%)
          Length = 1047

 Score = 61.6 bits (148), Expect = 3e-10
 Identities = 39/114 (34%), Positives = 65/114 (56%), Gaps = 1/114 (0%)
 Frame = +1

Query: 126 DTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSERKRRDKINEKIRALK 185
           +T++    +D     Q +   EKP     R +R       H+L+ER RR+KI+E++  L+
Sbjct: 229 NTNNKETCTDTSNSKQNSKASEKPDYIHVRARRGQATDS-HSLAERVRREKISERMNYLQ 405

Query: 186 ELIPNCNKM-DKASMLDDAIDYLKTLKLQLQVKFYVLQFSVTLFKLRFHYIILF 238
           +L+P CNK+  KA MLD+ I+Y+++  LQ QV+F  ++ +    +L F    LF
Sbjct: 406 DLVPGCNKVTGKAGMLDEIINYVQS--LQRQVEFLSMKLAAVNPRLDFSMDDLF 561


>TC218502 
          Length = 873

 Score = 60.8 bits (146), Expect = 5e-10
 Identities = 31/60 (51%), Positives = 43/60 (71%)
 Frame = +3

Query: 157 KRSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQV 216
           KRS  +A+ H ++ERKRR+K+++   AL  L+P   KMDKAS+L DAI Y+K LK +L V
Sbjct: 45  KRSPAHAQDHIMAERKRREKLSQSFIALAALVPGLKKMDKASVLGDAIKYVKELKERLTV 224


>TC231689 weakly similar to UP|Q38736 (Q38736) DEL, partial (22%)
          Length = 937

 Score = 60.8 bits (146), Expect = 5e-10
 Identities = 33/80 (41%), Positives = 51/80 (63%)
 Frame = +2

Query: 135 DNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSERKRRDKINEKIRALKELIPNCNKM 194
           D   E+QE    E   +EG RV+    N   H +SER+RR K+N++   L+ ++P+ +K 
Sbjct: 632 DGLHESQE----ENDYKEGMRVEAD-ENGMNHVMSERRRRAKLNQRFLTLRSMVPSISKD 796

Query: 195 DKASMLDDAIDYLKTLKLQL 214
           DK S+LDDAI+YLK L+ ++
Sbjct: 797 DKVSILDDAIEYLKKLERRI 856


>TC205597 similar to GB|AAO63377.1|28950907|BT005313 At4g34530 {Arabidopsis
           thaliana;} , partial (30%)
          Length = 1417

 Score = 60.5 bits (145), Expect = 7e-10
 Identities = 38/119 (31%), Positives = 71/119 (58%), Gaps = 1/119 (0%)
 Frame = +3

Query: 121 RKSHEDTDDSPYLSDNDEETQENIVKEKPVREGNRVKRSYRNAKVHNLSERKRRDKINEK 180
           + ++ +T  +    DN + ++  +  +KP     R +R       H+L+ER RR+KI+E+
Sbjct: 543 KNNNRETTSAETSKDNSKGSE--VQNQKPEYIHVRARRGQATDS-HSLAERVRREKISER 713

Query: 181 IRALKELIPNCNKM-DKASMLDDAIDYLKTLKLQLQVKFYVLQFSVTLFKLRFHYIILF 238
           ++ L++L+P CNK+  KA MLD+ I+Y+++  LQ QV+F  ++ +    +L F+   LF
Sbjct: 714 MKYLQDLVPGCNKVAGKAGMLDEIINYVQS--LQRQVEFLSMKLAAVNPRLDFNLDELF 884


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.315    0.130    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,231,434
Number of Sequences: 63676
Number of extensions: 109259
Number of successful extensions: 861
Number of sequences better than 10.0: 146
Number of HSP's better than 10.0 without gapping: 853
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 855
length of query: 240
length of database: 12,639,632
effective HSP length: 94
effective length of query: 146
effective length of database: 6,654,088
effective search space: 971496848
effective search space used: 971496848
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)


Medicago: description of AC139745.7