BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139744.6 + phase: 1 /partial
(384 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227038 similar to UP|AMP2_HUMAN (P50579) Methionine aminopepti... 417 e-117
TC227039 similar to UP|AMP2_HUMAN (P50579) Methionine aminopepti... 276 1e-74
TC227040 similar to UP|AMP2_HUMAN (P50579) Methionine aminopepti... 92 2e-29
AW308832 119 3e-27
TC226367 similar to UP|O22523 (O22523) DNA-binding protein GBP16... 106 2e-23
TC226369 similar to UP|O22523 (O22523) DNA-binding protein GBP16... 99 4e-21
TC232898 similar to UP|Q94AW9 (Q94AW9) At2g25350/F13B15.1 (Expre... 30 1.4
CD414853 similar to SP|P47923|NDK2_ Nucleoside diphosphate kinas... 30 1.4
TC230312 similar to UP|Q9XI34 (Q9XI34) F9L1.30, partial (5%) 30 1.4
BI941879 30 1.8
TC210477 similar to UP|Q94A51 (Q94A51) AT3g49060/T2J13_100, part... 30 2.4
TC218794 similar to PIR|T02456|T02456 protein kinase homolog F4I... 30 2.4
BU547394 30 2.4
TC221876 similar to UP|Q9LU01 (Q9LU01) Arabidopsis thaliana geno... 30 2.4
CD395254 similar to GP|17979464|gb| At1g68530/T26J14_10 {Arabido... 29 3.1
TC229419 UP|Q6PIN8 (Q6PIN8) TAO1 protein (Fragment), partial (5%) 29 3.1
TC219246 similar to GB|AAM98323.1|22655462|AY142059 At1g16810/F1... 29 4.1
CD407030 29 4.1
BE608452 28 5.3
>TC227038 similar to UP|AMP2_HUMAN (P50579) Methionine aminopeptidase 2
(MetAP 2) (Peptidase M 2) (Initiation factor 2
associated 67 kDa glycoprotein) (p67) (p67eIF2) ,
partial (55%)
Length = 1306
Score = 417 bits (1071), Expect = e-117
Identities = 215/380 (56%), Positives = 259/380 (67%)
Frame = +3
Query: 5 QKKKKNKKKKGKKVQTDPPTIPICELFSDGVYPIGQIMDYPTVNDSRTAKDRFTNEEKRA 64
+KK K+KKKK QTDPP+I + LF G +P G+I Y N RT T+EEKR
Sbjct: 297 KKKSKSKKKKELLQQTDPPSISVINLFPSGDFPEGEIQQYKDDNLWRT-----TSEEKRE 461
Query: 65 LDRSESDIYNEVRLAAEAHRQTRKHMQQWIKPGMTMIQICEELENTARKLIKEDGLKAGL 124
L+R + +YN VR AAE HRQ RK+++ +KPGM M ICE LENT RKLI EDGL+AG+
Sbjct: 462 LERLQKPLYNSVRRAAEVHRQVRKYIKGILKPGMLMTDICETLENTVRKLISEDGLQAGI 641
Query: 125 AFPTGCSRNHCAAHYTPNAGDPTVLEYDDVTKIDFGTHINGRIIDCAFTLSFNPKYDKLI 184
AFPTGCS N AAH+TPN+GD T+L+YDDV K+DFGTH++G I+DCAFT++FNP +D L+
Sbjct: 642 AFPTGCSLNWVAAHWTPNSGDKTILQYDDVMKLDFGTHVDGYIVDCAFTVAFNPMFDPLL 821
Query: 185 EAVRDATNTGIKAAGIDVPLCEIGAAIQEVMESYEVELDGKTYQVKSIRNLNGHSISPYR 244
EA R+ATNTGIK AGIDV LC+IGAAIQEVMESYEVE++GK YQVKSIRNLNGHSI Y+
Sbjct: 822 EASREATNTGIKEAGIDVRLCDIGAAIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQ 1001
Query: 245 IHAGKTVPIVKGGEATVMEENEYYAIETFGSTGRGQVHDDMDCSHYMKNFDAGYMPLRLQ 304
IHAGK+VPIVKGGE T MEE E++AIETF STG+G V +D++CSHYMKNFD
Sbjct: 1002IHAGKSVPIVKGGEQTKMEEGEFFAIETFASTGKGYVREDLECSHYMKNFD--------- 1154
Query: 305 SSKSLLSVINKNFSTLAFCKRWLDRAGCTKYQMALKDLCDKGIVDAYPPLCDVKGCYTAQ 364
LCD VKG Y +Q
Sbjct: 1155---------------------------------PYPTLCD------------VKGSYVSQ 1199
Query: 365 FEHTIMLRPTCKEVVSRGDD 384
FEHTI+LRPTCKEV+S+G D
Sbjct: 1200FEHTILLRPTCKEVISKGXD 1259
>TC227039 similar to UP|AMP2_HUMAN (P50579) Methionine aminopeptidase 2
(MetAP 2) (Peptidase M 2) (Initiation factor 2
associated 67 kDa glycoprotein) (p67) (p67eIF2) ,
partial (37%)
Length = 697
Score = 276 bits (706), Expect = 1e-74
Identities = 129/175 (73%), Positives = 151/175 (85%)
Frame = +3
Query: 210 AIQEVMESYEVELDGKTYQVKSIRNLNGHSISPYRIHAGKTVPIVKGGEATVMEENEYYA 269
AIQEVMESYEVE++GK YQVKSIRNLNGHSI Y+IHAGK+VPIVKGGE T MEE E++A
Sbjct: 3 AIQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQIHAGKSVPIVKGGEQTKMEEGEFFA 182
Query: 270 IETFGSTGRGQVHDDMDCSHYMKNFDAGYMPLRLQSSKSLLSVINKNFSTLAFCKRWLDR 329
IETF STG+G V +D++CSHYMKNFD G++PLRL +K LL+ INKNFSTLAFC+R+LDR
Sbjct: 183 IETFASTGKGYVREDLECSHYMKNFDVGHIPLRLPRAKQLLATINKNFSTLAFCRRYLDR 362
Query: 330 AGCTKYQMALKDLCDKGIVDAYPPLCDVKGCYTAQFEHTIMLRPTCKEVVSRGDD 384
G TKY MALK+LCD GIV YPPLCDVKG Y + FEHTI+LRPTC+EV+S+G D
Sbjct: 363 LGETKYLMALKNLCDSGIVQPYPPLCDVKGSYVSPFEHTILLRPTCQEVISKGAD 527
>TC227040 similar to UP|AMP2_HUMAN (P50579) Methionine aminopeptidase 2
(MetAP 2) (Peptidase M 2) (Initiation factor 2
associated 67 kDa glycoprotein) (p67) (p67eIF2) ,
partial (18%)
Length = 546
Score = 92.4 bits (228), Expect(2) = 2e-29
Identities = 39/51 (76%), Positives = 45/51 (87%)
Frame = +3
Query: 334 KYQMALKDLCDKGIVDAYPPLCDVKGCYTAQFEHTIMLRPTCKEVVSRGDD 384
KY MALK+LCD G+V YPPLCDVKG Y +QFEHTI+LRPTCKEV+S+GDD
Sbjct: 153 KYLMALKNLCDSGVVQPYPPLCDVKGSYVSQFEHTILLRPTCKEVISKGDD 305
Score = 54.7 bits (130), Expect(2) = 2e-29
Identities = 26/37 (70%), Positives = 30/37 (80%)
Frame = +1
Query: 302 RLQSSKSLLSVINKNFSTLAFCKRWLDRAGCTKYQMA 338
RL +K LL+ INKNFSTLAFC+R+LDR G TKY MA
Sbjct: 1 RLPRAKQLLATINKNFSTLAFCRRYLDRLGETKYLMA 111
>AW308832
Length = 451
Score = 119 bits (297), Expect = 3e-27
Identities = 68/154 (44%), Positives = 87/154 (56%)
Frame = +2
Query: 5 QKKKKNKKKKGKKVQTDPPTIPICELFSDGVYPIGQIMDYPTVNDSRTAKDRFTNEEKRA 64
+KK K+KKKK QTDPP+I + LF G P +I N RT T+ E R
Sbjct: 5 EKKSKSKKKKELLQQTDPPSIAVINLFPSGDVPESEIQQDKDDNLWRT-----TSRENRK 169
Query: 65 LDRSESDIYNEVRLAAEAHRQTRKHMQQWIKPGMTMIQICEELENTARKLIKEDGLKAGL 124
L+R +YN V AAE HRQ RK+++ +KP M M I + LENT R LI D L+A +
Sbjct: 170 LERLAKPLYNSVHQAAEIHRQVRKYIKSILKPRMLMTNIYKTLENTIRNLISYDNLQAYI 349
Query: 125 AFPTGCSRNHCAAHYTPNAGDPTVLEYDDVTKID 158
FPT S N HYTPN+ D T+L+Y + K D
Sbjct: 350 TFPTRYSLN*ITTHYTPNS*DNTILQYYYIMKFD 451
>TC226367 similar to UP|O22523 (O22523) DNA-binding protein GBP16, partial
(92%)
Length = 1233
Score = 106 bits (265), Expect = 2e-23
Identities = 88/326 (26%), Positives = 155/326 (46%), Gaps = 15/326 (4%)
Frame = +2
Query: 63 RALDRSESDIYNEVRLAAEAHRQTRKHMQQWIKPGMTMIQICEELENTAR-------KLI 115
+ LD S +++ + + AAE + K + KP ++ ICE+ ++ R K +
Sbjct: 2 KELDLSSAEVVTKYKTAAEIVNKALKLVISECKPKAKIVDICEKGDSYIREQTGNVYKNV 181
Query: 116 KEDGLKAGLAFPTGCSRNHCAAHYTPNAGDPTVLEYDDVTKIDFGTHINGRIIDCAFT-- 173
K ++ G+AFPT S N+ H++P A D VLE D+ KID HI+G I A T
Sbjct: 182 KRK-IERGVAFPTCLSINNVVCHFSPLASDEAVLEEGDILKIDMACHIDGFIAVVAHTHV 358
Query: 174 LSFNPKYDKLIEAVRDATNTGIKAAGIDVPLCEIGAAIQEVMESYEVELDGKTYQVKSIR 233
L P + + + A NT AA + + L G ++V ++ ++ Y K +
Sbjct: 359 LQEGPVTGRAADVIA-AANT---AAEVALRLVRPGKKNKDVSDA--IQKVAAAYDCKIVE 520
Query: 234 NLNGHSISPYRIHAGKTVPIVKGGEATV----MEENEYYAIETFGSTGRG--QVHDDMDC 287
+ H + + I K V + + V EENE YAI+ STG G ++ D+
Sbjct: 521 GVLSHQMKQFVIDGNKVVLSLSNPDTRVDEAEFEENEVYAIDIVTSTGDGKPKLLDEKQT 700
Query: 288 SHYMKNFDAGYMPLRLQSSKSLLSVINKNFSTLAFCKRWLDRAGCTKYQMALKDLCDKGI 347
+ Y + D Y L++++S+ + S I++ F + F R L+ + ++ L + + +
Sbjct: 701 TIYKRAVDKSY-HLKMKASRFIFSEISQKFPIMPFSARALEE---KRARLGLVECVNHEL 868
Query: 348 VDAYPPLCDVKGCYTAQFEHTIMLRP 373
+ YP L + G + A + T++L P
Sbjct: 869 LQPYPVLHEKPGDFVAHIKFTVLLMP 946
>TC226369 similar to UP|O22523 (O22523) DNA-binding protein GBP16, partial
(77%)
Length = 1128
Score = 98.6 bits (244), Expect = 4e-21
Identities = 81/280 (28%), Positives = 135/280 (47%), Gaps = 15/280 (5%)
Frame = +3
Query: 61 EKRALDRSESDIYNEVRLAAEAHRQTRKHMQQWIKPGMTMIQICEELENTAR-------K 113
EK+ LD + ++ + + AAE + + + KP ++ +CE+ ++ R K
Sbjct: 120 EKKELDLTSPEVVTKYKSAAEIVNKALQLVISECKPKTKIVDLCEKGDSYIREQTGNMYK 299
Query: 114 LIKEDGLKAGLAFPTGCSRNHCAAHYTPNAGDPTVLEYDDVTKIDFGTHINGRIIDCAFT 173
+K ++ G+AFPT S N+ H++P A D TVLE D+ KID HI+G I A T
Sbjct: 300 NVKRK-IERGVAFPTCVSVNNTVCHFSPLASDETVLEDGDIVKIDMACHIDGFIAAVAHT 476
Query: 174 --LSFNPKYDKLIEAVRDATNTGIKAAGIDVPLCEIGAAIQEVMESYEVELDGKTYQVKS 231
L P + +A+ A NT AA + + L G ++V E+ ++ Y K
Sbjct: 477 HVLQEGPVTGRAADALA-AANT---AAEVALRLVRPGRKNKDVTEA--IQKIAAAYDCKI 638
Query: 232 IRNLNGHSISPYRIHAGKTVPIVKGGEATV----MEENEYYAIETFGSTGRG--QVHDDM 285
+ + H + + I K V V + V EENE YAI+ STG G ++ D+
Sbjct: 639 VEGVLSHQMKQFVIDGNKVVLSVSNPDTRVDDAEFEENEVYAIDIVASTGDGKPKLLDEK 818
Query: 286 DCSHYMKNFDAGYMPLRLQSSKSLLSVINKNFSTLAFCKR 325
+ Y + D Y L++++S+ + S I++ F + F R
Sbjct: 819 QTTIYKRAVDKSY-HLKMKASRFIFSEISQKFPIMPFSAR 935
>TC232898 similar to UP|Q94AW9 (Q94AW9) At2g25350/F13B15.1 (Expressed
protein), partial (74%)
Length = 797
Score = 30.4 bits (67), Expect = 1.4
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = -2
Query: 187 VRDATNTGIKAAGIDVPLCEIGAAIQEVMESYEVELD 223
V D+ +T IK G+D PLC IG+ V +++ LD
Sbjct: 652 VLDSFSTKIKHEGVDNPLCPIGSGASLVESAHQDYLD 542
>CD414853 similar to SP|P47923|NDK2_ Nucleoside diphosphate kinase II
chloroplast precursor (EC 2.7.4.6) (NDK II) (NDP kinase
II), partial (20%)
Length = 446
Score = 30.4 bits (67), Expect = 1.4
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = -2
Query: 5 QKKKKNKKKKGKKVQTDPPTIPICELFSDG 34
+KKKK KKKK KK++T ++P E + G
Sbjct: 424 KKKKKKKKKKKKKLETSSLSLPRAEFGTRG 335
>TC230312 similar to UP|Q9XI34 (Q9XI34) F9L1.30, partial (5%)
Length = 701
Score = 30.4 bits (67), Expect = 1.4
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = +2
Query: 4 AQKKKKNKKKKGKKVQTDPPTIPI 27
++K+KKNKKK KK+++ P PI
Sbjct: 122 SKKRKKNKKKSNKKLRSQTPLSPI 193
>BI941879
Length = 257
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +3
Query: 5 QKKKKNKKKKGKKVQTDPPTIP 26
+KKKK KKKKG V DP +P
Sbjct: 51 KKKKKKKKKKGAAVLKDPSLLP 116
>TC210477 similar to UP|Q94A51 (Q94A51) AT3g49060/T2J13_100, partial (9%)
Length = 757
Score = 29.6 bits (65), Expect = 2.4
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = +3
Query: 211 IQEVMESYEVELDGKTYQVKSIRNL--NGHSISP 242
+QEVME + DG TY+ ++IR +GH SP
Sbjct: 87 VQEVMEDPYIAADGFTYEEEAIRGWLNSGHDTSP 188
>TC218794 similar to PIR|T02456|T02456 protein kinase homolog F4I18.11 -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(30%)
Length = 998
Score = 29.6 bits (65), Expect = 2.4
Identities = 23/71 (32%), Positives = 36/71 (50%), Gaps = 10/71 (14%)
Frame = +2
Query: 182 KLIEAVR----DATNTGIKAAGIDVP----LCEIGAAIQEVMESYEVELDGKTYQVKSIR 233
++++A+R A + G+ + G+ P +C I QEVM V DG TY+ ++IR
Sbjct: 497 RVLDAMRVSSGGANSFGLSSEGLLQPPSYFICPI---FQEVMRDPHVAADGFTYEAEAIR 667
Query: 234 NL--NGHSISP 242
GH SP
Sbjct: 668 GWLDGGHDNSP 700
>BU547394
Length = 648
Score = 29.6 bits (65), Expect = 2.4
Identities = 18/41 (43%), Positives = 22/41 (52%), Gaps = 2/41 (4%)
Frame = -1
Query: 204 LCEIGAAIQEVMESYEVELDGKTYQVKSIRNL--NGHSISP 242
LC I QE+M +V DG TY+ K+I NGH SP
Sbjct: 384 LCPI---FQEIMHDPQVAADGFTYEGKAISEWLENGHETSP 271
>TC221876 similar to UP|Q9LU01 (Q9LU01) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K15C23, partial (22%)
Length = 468
Score = 29.6 bits (65), Expect = 2.4
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = -1
Query: 1 GGEAQKKKKNKKKKGKKVQTDPPTIPICEL 30
GG +KK+ KKKKGKK T+P C +
Sbjct: 90 GGGRWEKKEKKKKKGKKAVAV*VTMPWCRI 1
>CD395254 similar to GP|17979464|gb| At1g68530/T26J14_10 {Arabidopsis
thaliana}, partial (9%)
Length = 670
Score = 29.3 bits (64), Expect = 3.1
Identities = 15/36 (41%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Frame = -2
Query: 5 QKKKKNKKKKGKKVQTDPPTI-PICELFSDGVYPIG 39
+KKKK KKKK KK +T ++ P C + +G G
Sbjct: 405 KKKKKKKKKKKKKKKTQTSSLSPSCRIRHEGQIAFG 298
>TC229419 UP|Q6PIN8 (Q6PIN8) TAO1 protein (Fragment), partial (5%)
Length = 895
Score = 29.3 bits (64), Expect = 3.1
Identities = 17/37 (45%), Positives = 22/37 (58%), Gaps = 1/37 (2%)
Frame = +3
Query: 5 QKKKKNKKKKGKKVQTDPPTIPICELFSDG-VYPIGQ 40
+KKKK KKKK KK + PP + FS G +P G+
Sbjct: 696 KKKKKKKKKKKKKKKKKPPAL----FFSPGFAFPPGK 794
>TC219246 similar to GB|AAM98323.1|22655462|AY142059 At1g16810/F17F16.8
{Arabidopsis thaliana;} , partial (67%)
Length = 808
Score = 28.9 bits (63), Expect = 4.1
Identities = 24/99 (24%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Frame = +2
Query: 1 GGEAQKKKKNKKKKGKKVQTDPPTIPICELFSDGVYP-IGQIMDYPTVN-DSRTA--KDR 56
GG +KKKKNK+ + + +Q + P G I D ++ +TA D
Sbjct: 221 GGVKKKKKKNKRNQEQLLQVAEDEVSAGGNTEQAKDPNDGDINDVSELSGKGKTAHYDDH 400
Query: 57 FTNEEKRALD-RSESDIYNEVRLAAEAHRQTRKHMQQWI 94
T E+R ++ R + D++ +++ ++HR + Q++
Sbjct: 401 LTPAERRYIEQREQLDVHRLAKISNKSHRDRIQDFNQYL 517
>CD407030
Length = 555
Score = 28.9 bits (63), Expect = 4.1
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -1
Query: 2 GEAQKKKKNKKKKGKKVQTDPPTIPICELFSDGVY 36
G+ +KKKK KKKK KK P C + +G +
Sbjct: 402 GKKKKKKKKKKKKKKKKTQIVSLSPSCRIRHEGSF 298
>BE608452
Length = 426
Score = 28.5 bits (62), Expect = 5.3
Identities = 19/70 (27%), Positives = 34/70 (48%), Gaps = 6/70 (8%)
Frame = +3
Query: 6 KKKKNKKKKGKKVQ--TDPPTIPIC-ELFSDGVYPI---GQIMDYPTVNDSRTAKDRFTN 59
+KKK K+K+ KK++ + P + +C + D V PI + + T N + T K +
Sbjct: 138 EKKKRKRKRKKKIEESNEQPQVEVCPKSLVDSVIPIVAQKENAETTTENSNPTPKKKKKK 317
Query: 60 EEKRALDRSE 69
+ K D +
Sbjct: 318 KNKNVQDAEQ 347
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.135 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,744,040
Number of Sequences: 63676
Number of extensions: 226200
Number of successful extensions: 5632
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1732
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4442
length of query: 384
length of database: 12,639,632
effective HSP length: 99
effective length of query: 285
effective length of database: 6,335,708
effective search space: 1805676780
effective search space used: 1805676780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC139744.6