BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139743.4 - phase: 0 /pseudo
(105 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216154 similar to UP|Q9LLM3 (Q9LLM3) MTD1, partial (40%) 66 3e-12
TC216153 similar to UP|Q9LLM3 (Q9LLM3) MTD1, partial (61%) 64 9e-12
AI496382 similar to GP|9294810|gb|A MTD1 {Medicago truncatula}, ... 43 2e-05
TC218124 40 2e-04
TC215968 similar to UP|Q9LLM3 (Q9LLM3) MTD1, partial (19%) 36 0.003
TC215967 weakly similar to GB|AAP13408.1|30023750|BT006300 At5g2... 35 0.006
BE059564 35 0.007
BE657503 similar to SP|P31235|CAQ2_ Calsequestrin cardiac muscl... 34 0.010
TC215289 weakly similar to UP|Q9SJB0 (Q9SJB0) Expressed protein,... 33 0.028
TC215290 weakly similar to UP|Q9SJB0 (Q9SJB0) Expressed protein,... 31 0.082
BU091346 31 0.082
TC210776 similar to UP|Q8H6B1 (Q8H6B1) Global transcription fact... 31 0.082
TC203877 similar to UP|ENPL_CATRO (P35016) Endoplasmin homolog p... 31 0.11
BQ612102 weakly similar to GP|16974560|gb| At2g02870/T17M13.4 {A... 31 0.11
TC234991 similar to UP|Q6YTC8 (Q6YTC8) Zinc finger (C3HC4-type R... 30 0.14
BF066654 30 0.14
TC232681 weakly similar to GB|AAP21377.1|30102918|BT006569 At1g4... 29 0.31
TC225618 similar to UP|Q9LSQ5 (Q9LSQ5) 1,4-benzoquinone reductas... 29 0.41
TC234663 29 0.41
TC232838 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {A... 29 0.41
>TC216154 similar to UP|Q9LLM3 (Q9LLM3) MTD1, partial (40%)
Length = 449
Score = 65.9 bits (159), Expect = 3e-12
Identities = 44/76 (57%), Positives = 51/76 (66%), Gaps = 2/76 (2%)
Frame = +1
Query: 13 SRTSVLFDAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMD--ENEAESKYNG 70
SR +VL AP + D + C++TSSSSIGRNSD VSSERSM+ ENE ES Y+
Sbjct: 76 SRAAVLLHAPEE-DAAAEECCTSTSSSSIGRNSD------VSSERSMEEGENEVESAYH- 231
Query: 71 GALDCMEALEEVLPIR 86
G L ME LEEVLPIR
Sbjct: 232 GPLHAMETLEEVLPIR 279
>TC216153 similar to UP|Q9LLM3 (Q9LLM3) MTD1, partial (61%)
Length = 1021
Score = 64.3 bits (155), Expect = 9e-12
Identities = 42/76 (55%), Positives = 51/76 (66%), Gaps = 2/76 (2%)
Frame = +2
Query: 13 SRTSVLFDAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMD--ENEAESKYNG 70
SR +VL A + +++ CST++SSSIGRNSD VSSERSM+ ENE ES Y+
Sbjct: 107 SRAAVLLHAQEEEEEE----CSTSTSSSIGRNSD------VSSERSMEEGENEVESAYH- 253
Query: 71 GALDCMEALEEVLPIR 86
G L ME LEEVLPIR
Sbjct: 254 GPLHAMETLEEVLPIR 301
>AI496382 similar to GP|9294810|gb|A MTD1 {Medicago truncatula}, partial
(32%)
Length = 410
Score = 43.1 bits (100), Expect = 2e-05
Identities = 26/67 (38%), Positives = 40/67 (58%), Gaps = 1/67 (1%)
Frame = +2
Query: 21 APRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESKYN-GGALDCMEAL 79
+P +D + CST+++SSIG +SD + E ++NEA S Y LD M++L
Sbjct: 32 SPSFEVEDDNEHCSTSATSSIGNDSDVLSETECGE----NDNEAHSTYKCHQPLDTMDSL 199
Query: 80 EEVLPIR 86
++VLPIR
Sbjct: 200QQVLPIR 220
>TC218124
Length = 658
Score = 40.0 bits (92), Expect = 2e-04
Identities = 28/70 (40%), Positives = 40/70 (57%), Gaps = 12/70 (17%)
Frame = +1
Query: 34 STTSSSSIGR--NSDDDDDDEVSSERSM----------DENEAESKYNGGALDCMEALEE 81
S+ S SSIG +SDDD++D VSS+R+ +E E SK G L +++LE+
Sbjct: 313 SSDSESSIGTPDDSDDDENDVVSSKRNQSDEDEDKEEEEEEEVHSKLKG--LASLDSLED 486
Query: 82 VLPIRLFQSF 91
LPI+L F
Sbjct: 487 SLPIKLVLQF 516
>TC215968 similar to UP|Q9LLM3 (Q9LLM3) MTD1, partial (19%)
Length = 942
Score = 36.2 bits (82), Expect = 0.003
Identities = 25/67 (37%), Positives = 33/67 (48%)
Frame = +1
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESKYNGGALDCMEAL 79
D P D D ++S SSIGRNS +D S E E +S + G LD + L
Sbjct: 154 DFPADDSD-------SSSDSSIGRNSTSSEDS--SDREDAGEVEVQSSFK-GPLDTINDL 303
Query: 80 EEVLPIR 86
EE LP++
Sbjct: 304 EEDLPVK 324
>TC215967 weakly similar to GB|AAP13408.1|30023750|BT006300 At5g21940
{Arabidopsis thaliana;} , partial (26%)
Length = 1034
Score = 35.0 bits (79), Expect = 0.006
Identities = 25/67 (37%), Positives = 33/67 (48%)
Frame = +2
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESKYNGGALDCMEAL 79
D P D D ++S SSIGRNS +D S E E +S + G LD + L
Sbjct: 158 DFPEDDSD-------SSSDSSIGRNSISSEDS--SDREDAVEVEVQSSFK-GPLDTINDL 307
Query: 80 EEVLPIR 86
EE LP++
Sbjct: 308 EEDLPVK 328
>BE059564
Length = 406
Score = 34.7 bits (78), Expect = 0.007
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +3
Query: 19 FDAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESKY 68
F+ P D+D++ + + G + DDDDDDE E D+++ + +Y
Sbjct: 114 FELPHDYDEN-AEANLIEIPADFGLSHDDDDDDETDDEEEDDDDDEDEEY 260
>BE657503 similar to SP|P31235|CAQ2_ Calsequestrin cardiac muscle isoform
precursor (Calsequestrin 2). [Rabbit] {Oryctolagus
cuniculus}, partial (4%)
Length = 662
Score = 34.3 bits (77), Expect = 0.010
Identities = 19/50 (38%), Positives = 25/50 (50%)
Frame = -2
Query: 11 VGSRTSVLFDAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMD 60
VG + D D DDD GK+ IG NSD D E ++E++MD
Sbjct: 268 VGKEDMDIDDNVDDDDDDNGKIDLDVEGYIIGGNSDARDRKEENAEQNMD 119
>TC215289 weakly similar to UP|Q9SJB0 (Q9SJB0) Expressed protein, partial
(32%)
Length = 1476
Score = 32.7 bits (73), Expect = 0.028
Identities = 21/51 (41%), Positives = 31/51 (60%)
Frame = +3
Query: 37 SSSSIGRNSDDDDDDEVSSERSMDENEAESKYNGGALDCMEALEEVLPIRL 87
SSSSIG D D+++E +E +++ K G L ++ALEE LPI+L
Sbjct: 360 SSSSIGTPDDSDNEEE-------EEVQSQLKIRTG-LGSLDALEESLPIKL 488
>TC215290 weakly similar to UP|Q9SJB0 (Q9SJB0) Expressed protein, partial
(36%)
Length = 820
Score = 31.2 bits (69), Expect = 0.082
Identities = 20/50 (40%), Positives = 30/50 (60%)
Frame = +3
Query: 37 SSSSIGRNSDDDDDDEVSSERSMDENEAESKYNGGALDCMEALEEVLPIR 86
SSSSIG D D+++E +E +++ K G L ++ALEE LPI+
Sbjct: 345 SSSSIGTPDDSDNEEE-------EEVQSQLKIRTG-LGSLDALEESLPIK 470
>BU091346
Length = 431
Score = 31.2 bits (69), Expect = 0.082
Identities = 20/50 (40%), Positives = 30/50 (60%)
Frame = +1
Query: 37 SSSSIGRNSDDDDDDEVSSERSMDENEAESKYNGGALDCMEALEEVLPIR 86
SSSSIG D D+++E +E +++ K G L ++ALEE LPI+
Sbjct: 235 SSSSIGTPDDSDNEEE-------EEVQSQLKIRTG-LGSLDALEESLPIK 360
>TC210776 similar to UP|Q8H6B1 (Q8H6B1) Global transcription factor group C
102, partial (12%)
Length = 411
Score = 31.2 bits (69), Expect = 0.082
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +1
Query: 22 PRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDE 61
P D + + + S S+ + DDDDDDE S E S +E
Sbjct: 136 PSDVEPESDSEDEASDSESLVESEDDDDDDEDSEEDSEEE 255
>TC203877 similar to UP|ENPL_CATRO (P35016) Endoplasmin homolog precursor
(GRP94 homolog), partial (65%)
Length = 2071
Score = 30.8 bits (68), Expect = 0.11
Identities = 25/84 (29%), Positives = 37/84 (43%), Gaps = 2/84 (2%)
Frame = +1
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEA--ESKYNGGALDCME 77
+ P D DD + S+ SSS S+D+D D+ E + + E+ Y E
Sbjct: 160 EVPADEDDSSDEEDSSESSSK--EESEDEDADKSEDEEKKPKTKTVKETTYE------WE 315
Query: 78 ALEEVLPIRLFQSFMVMQEKYLKF 101
L +V I L V +E+Y KF
Sbjct: 316 LLNDVKAIWLRNPKEVTEEEYTKF 387
>BQ612102 weakly similar to GP|16974560|gb| At2g02870/T17M13.4 {Arabidopsis
thaliana}, partial (16%)
Length = 444
Score = 30.8 bits (68), Expect = 0.11
Identities = 16/52 (30%), Positives = 25/52 (47%), Gaps = 4/52 (7%)
Frame = +1
Query: 24 DHDDDQGKVCSTTSSSSIGRNSDDDD----DDEVSSERSMDENEAESKYNGG 71
DH D+ + T SS G D+D DDE S ++E + +++GG
Sbjct: 55 DHQSDKQQQAQTGDSSDFGAQLSDEDQLQHDDENLLNSSEQQSEGQQQHHGG 210
>TC234991 similar to UP|Q6YTC8 (Q6YTC8) Zinc finger (C3HC4-type RING
finger)-like protein, partial (19%)
Length = 524
Score = 30.4 bits (67), Expect = 0.14
Identities = 15/27 (55%), Positives = 20/27 (73%)
Frame = -1
Query: 27 DDQGKVCSTTSSSSIGRNSDDDDDDEV 53
++ G+ CS++SSSS N DDDDDD V
Sbjct: 311 ENGGEGCSSSSSSS-SSNKDDDDDDVV 234
>BF066654
Length = 394
Score = 30.4 bits (67), Expect = 0.14
Identities = 17/40 (42%), Positives = 25/40 (62%)
Frame = +2
Query: 27 DDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAES 66
D+ GKVCS++SSSS N DDD V +E++A++
Sbjct: 65 DNGGKVCSSSSSSS-SSNKDDDVVVVVECRICQEEDQAQA 181
>TC232681 weakly similar to GB|AAP21377.1|30102918|BT006569 At1g47970
{Arabidopsis thaliana;} , partial (39%)
Length = 867
Score = 29.3 bits (64), Expect = 0.31
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = +1
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESK 67
DAP DDD + S G DDDD+D+ + ++ E + +
Sbjct: 256 DAPDGGDDDDDEDEEEESDVQRGGEPDDDDNDDDDEDEDEEDEEEQGE 399
Score = 24.6 bits (52), Expect = 7.6
Identities = 10/33 (30%), Positives = 18/33 (54%)
Frame = +1
Query: 24 DHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSE 56
D DD++ + + G + DDD+D+E S+
Sbjct: 214 DDDDNEDEEDDDEDDAPDGGDDDDDEDEEEESD 312
>TC225618 similar to UP|Q9LSQ5 (Q9LSQ5) 1,4-benzoquinone reductase-like; Trp
repressor binding protein-like (1,4-benzoquinone
reductase-like protein), partial (75%)
Length = 1864
Score = 28.9 bits (63), Expect = 0.41
Identities = 16/58 (27%), Positives = 24/58 (40%)
Frame = -2
Query: 26 DDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESKYNGGALDCMEALEEVL 83
+D STT +N DDDDDD+ + N + N + E L+ +L
Sbjct: 759 NDPTNNNTSTTGRKRRRKNEDDDDDDDDGDDDGSSNNNNNNNNNKRSKKKKEELKGIL 586
>TC234663
Length = 773
Score = 28.9 bits (63), Expect = 0.41
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 6/50 (12%)
Frame = -2
Query: 35 TTSSSSIGRNSDDD------DDDEVSSERSMDENEAESKYNGGALDCMEA 78
T SSSSIG +DD DD E SS S D++ S + G C+ +
Sbjct: 325 TFSSSSIGIFDEDDTEEDRHDDQENSSGNSPDDDSTTSGGDVGMFGCLNS 176
>TC232838 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;} , partial (46%)
Length = 902
Score = 28.9 bits (63), Expect = 0.41
Identities = 17/52 (32%), Positives = 24/52 (45%), Gaps = 1/52 (1%)
Frame = +2
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDD-EVSSERSMDENEAESKYNG 70
DAP DDD + G DDDD+D + S+ DE+E + + G
Sbjct: 401 DAPDGGDDDDDEDDEEEGDVQRGGEPDDDDNDSDDDSDDDEDEDEEDEEEQG 556
Score = 27.3 bits (59), Expect = 1.2
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = +2
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDEVSSERSMDENEAESK 67
D + D+++G V +SDDD DD+ + +E + E +
Sbjct: 422 DDDDEDDEEEGDVQRGGEPDDDDNDSDDDSDDDEDEDEEDEEEQGEEE 565
Score = 25.8 bits (55), Expect = 3.4
Identities = 11/33 (33%), Positives = 15/33 (45%)
Frame = +2
Query: 20 DAPRDHDDDQGKVCSTTSSSSIGRNSDDDDDDE 52
D D DDD+G + DDDD+D+
Sbjct: 344 DEEEDDDDDEGDDNDDEEDDAPDGGDDDDDEDD 442
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.312 0.129 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,759,886
Number of Sequences: 63676
Number of extensions: 41260
Number of successful extensions: 764
Number of sequences better than 10.0: 188
Number of HSP's better than 10.0 without gapping: 508
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of query: 105
length of database: 12,639,632
effective HSP length: 81
effective length of query: 24
effective length of database: 7,481,876
effective search space: 179565024
effective search space used: 179565024
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC139743.4