BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139708.3 + phase: 0
(150 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227625 149 4e-37
TC204460 homologue to UP|B2_DAUCA (P37707) B2 protein, partial (... 74 3e-14
BM521768 72 1e-13
TC204457 Glycine max differential display clone 1/51 72 1e-13
TC215135 similar to GB|AAM65351.1|21655279|AY103299 AT3g27090/MO... 70 3e-13
TC215134 homologue to UP|Q8L6E9 (Q8L6E9) GDA2 protein, partial (... 55 1e-08
AW348385 similar to GP|21665866|em GDA2 protein {Pisum sativum},... 46 8e-06
TC204459 homologue to UP|Q9AYM6 (Q9AYM6) CPRD48 protein (Fragmen... 32 0.11
TC228622 similar to UP|Q9XIS3 (Q9XIS3) Lectin-like protein kinas... 29 0.73
TC204458 29 0.96
TC205631 weakly similar to UP|CBR_DUNBA (P27516) Carotene biosyn... 28 1.6
BM086669 28 1.6
TC222342 homologue to UP|Q93W01 (Q93W01) At2g01080/F23H14.5, par... 28 1.6
CO980352 28 2.1
TC227624 27 3.6
BG550714 similar to GP|18146784|db PBng110 {Vigna radiata}, part... 27 4.8
TC212194 similar to UP|Q9LK97 (Q9LK97) Exonuclease-like protein,... 27 4.8
TC213877 27 4.8
CO980758 27 4.8
BI317616 27 4.8
>TC227625
Length = 1751
Score = 149 bits (377), Expect = 4e-37
Identities = 72/126 (57%), Positives = 89/126 (70%)
Frame = +3
Query: 13 KLAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATS 72
K +GFIFMC+ TKP+CF YRV + A +K IV INPG LFL+D D++LLYGIY A+S
Sbjct: 429 KPSGFIFMCSAKTKPDCFRYRVMGVSATKKDIVLSINPGTKLFLYDFDLRLLYGIYKASS 608
Query: 73 TGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNIRQVSS 132
+G + +EP AFGG FPAQV FKI DC PLPE+ FK AI++NY + +KF EL RQV
Sbjct: 609 SGGMKLEPRAFGGNFPAQVRFKIASDCFPLPESIFKKAIQENYNEKHKFKTELTARQVRK 788
Query: 133 AIVTFR 138
FR
Sbjct: 789 LTELFR 806
>TC204460 homologue to UP|B2_DAUCA (P37707) B2 protein, partial (71%)
Length = 942
Score = 73.9 bits (180), Expect = 3e-14
Identities = 48/136 (35%), Positives = 68/136 (49%), Gaps = 10/136 (7%)
Frame = +1
Query: 12 DKLAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLAT 71
+ + G+IF+CN T E ++F LP + V I PG+ LFL++ L+GI+ A
Sbjct: 79 ETIGGYIFVCNNDTMAENLKRQLFGLPPRYRDSVRAITPGLPLFLYNYSTHQLHGIFEAA 258
Query: 72 STGMLNIEPYAF-------GGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KF 121
S G NI+P A+ RFPAQV K C PL E+ F+ + HY KF
Sbjct: 259 SFGGSNIDPSAWEDKKCPGESRFPAQVRVITRKTCEPLEEDSFRPIL-----HHYDGPKF 423
Query: 122 NPELNIRQVSSAIVTF 137
ELN+ + S + F
Sbjct: 424 RLELNVPEALSLLDIF 471
>BM521768
Length = 429
Score = 72.0 bits (175), Expect = 1e-13
Identities = 34/60 (56%), Positives = 38/60 (62%)
Frame = +2
Query: 79 EPYAFGGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHYKFNPELNIRQVSSAIVTFR 138
EP AFGG FPAQV F + DC PLPE+ FK AIK+NY + KF EL RQV FR
Sbjct: 2 EPRAFGGNFPAQVRFNVASDCFPLPESIFKKAIKENYNEKNKFKTELTARQVKKLTELFR 181
>TC204457 Glycine max differential display clone 1/51
Length = 1464
Score = 71.6 bits (174), Expect = 1e-13
Identities = 47/136 (34%), Positives = 68/136 (49%), Gaps = 10/136 (7%)
Frame = +3
Query: 12 DKLAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLAT 71
+ + G+IF+CN T E ++F LP + V I PG+ LFL++ L+GI+ A
Sbjct: 732 ETIGGYIFVCNNDTMAENLQRQLFGLPPRYRDSVRTITPGLPLFLYNYSTHQLHGIFEAA 911
Query: 72 STGMLNIEPYAF-------GGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KF 121
S G NI+P A+ RFPAQV K C PL E+ F+ + HY KF
Sbjct: 912 SFGGSNIDPTAWEDKKCPGESRFPAQVQVITRKVCEPLEEDSFRPIL-----HHYDGPKF 1076
Query: 122 NPELNIRQVSSAIVTF 137
EL++ + S + F
Sbjct: 1077RLELSVPEALSLLDIF 1124
>TC215135 similar to GB|AAM65351.1|21655279|AY103299 AT3g27090/MOJ10_18
{Arabidopsis thaliana;} , partial (74%)
Length = 1358
Score = 70.5 bits (171), Expect = 3e-13
Identities = 45/124 (36%), Positives = 64/124 (51%), Gaps = 10/124 (8%)
Frame = +3
Query: 14 LAGFIFMCNRMTKPECFSYRVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATST 73
L G+IF+CN T E ++F LP + V I PG+ LFL++ L+GI+ A S
Sbjct: 549 LGGYIFVCNNDTMQEDLKRQLFGLPPRYRDSVRAITPGLPLFLYNYTTHQLHGIFEAASF 728
Query: 74 GMLNIEPYAF-------GGRFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KFNP 123
G NI+P A+ RFPAQV ++ K C L E+ F+ + HY KF
Sbjct: 729 GGSNIDPTAWEDKKCKGESRFPAQVRIRVRKLCKALEEDSFRPVL-----HHYDGPKFRL 893
Query: 124 ELNI 127
EL++
Sbjct: 894 ELSV 905
>TC215134 homologue to UP|Q8L6E9 (Q8L6E9) GDA2 protein, partial (57%)
Length = 779
Score = 55.5 bits (132), Expect = 1e-08
Identities = 37/105 (35%), Positives = 54/105 (51%), Gaps = 10/105 (9%)
Frame = +1
Query: 33 RVFALPAGRKHIVERINPGMHLFLFDTDVKLLYGIYLATSTGMLNIEPYAF-------GG 85
++F LP + V I PG+ LFL++ L+GI+ A S G NI+P A+
Sbjct: 31 QLFGLPPRYRDSVRAITPGLPLFLYNYTTHQLHGIFEAASFGGSNIDPTAWEDKKCKGES 210
Query: 86 RFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KFNPELNI 127
RFPAQV ++ K C L E+ F+ + HY KF EL++
Sbjct: 211 RFPAQVRIRVRKLCKALEEDSFRPVL-----HHYDGPKFRLELSV 330
>AW348385 similar to GP|21665866|em GDA2 protein {Pisum sativum}, partial
(49%)
Length = 564
Score = 45.8 bits (107), Expect = 8e-06
Identities = 27/71 (38%), Positives = 36/71 (50%), Gaps = 7/71 (9%)
Frame = -1
Query: 45 VERINPGMHLFLFDTDVKLLYGIYLATSTGMLNIEPYAF-------GGRFPAQVSFKILK 97
V I PG+ LFL+ L+GI+ A S G NI+P A RFP QV ++ K
Sbjct: 564 VRAITPGLLLFLYXXTTHQLHGIFEAASFGGSNIDPTAXEDKKCKGESRFPXQVRIRVRK 385
Query: 98 DCLPLPENDFK 108
C L E+ F+
Sbjct: 384 LCKALEEDSFR 352
>TC204459 homologue to UP|Q9AYM6 (Q9AYM6) CPRD48 protein (Fragment), partial
(97%)
Length = 621
Score = 32.0 bits (71), Expect = 0.11
Identities = 21/55 (38%), Positives = 27/55 (48%), Gaps = 3/55 (5%)
Frame = +2
Query: 86 RFPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KFNPELNIRQVSSAIVTF 137
RFPAQV K C PL E+ F+ + HY KF ELN+ + S + F
Sbjct: 44 RFPAQVRVITRKTCEPLEEDSFRPIL-----HHYDGPKFRLELNVPEALSLLDIF 193
>TC228622 similar to UP|Q9XIS3 (Q9XIS3) Lectin-like protein kinase, partial
(29%)
Length = 1036
Score = 29.3 bits (64), Expect = 0.73
Identities = 14/52 (26%), Positives = 23/52 (43%), Gaps = 3/52 (5%)
Frame = +3
Query: 101 PLPENDFKHAIKDNYQKHYKFNPELNI---RQVSSAIVTFRFCFCFLCFAFS 149
P ++ D + H +F+ ++ + S TF F FCFLC +S
Sbjct: 654 PTTTTEYTPMSSDTHSMHVQFSDSNSLI*NNLIPSLKTTFSFSFCFLCLCWS 809
>TC204458
Length = 551
Score = 28.9 bits (63), Expect = 0.96
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 10/71 (14%)
Frame = +1
Query: 77 NIEPYAFGGR-------FPAQVSFKILKDCLPLPENDFKHAIKDNYQKHY---KFNPELN 126
NI+P A+ + FPAQV K C PL E+ F+ + HY KF EL+
Sbjct: 7 NIDPTAWEDKKCPGESGFPAQVQVITRKVCEPLEEDSFRPIL-----HHYDGPKFRLELS 171
Query: 127 IRQVSSAIVTF 137
+ + S + F
Sbjct: 172 VPEALSLLDIF 204
>TC205631 weakly similar to UP|CBR_DUNBA (P27516) Carotene
biosynthesis-related protein CBR, chloroplast precursor,
partial (17%)
Length = 1088
Score = 28.1 bits (61), Expect = 1.6
Identities = 20/73 (27%), Positives = 31/73 (42%), Gaps = 8/73 (10%)
Frame = +2
Query: 81 YAFGGRFPAQVSFKILKDCLPLPENDFKHA--------IKDNYQKHYKFNPELNIRQVSS 132
Y F P + +F + PLP F A ++D++ + KF + R+
Sbjct: 86 YPFQFHAPLKPTFSVFP---PLPNPSFSAATLCSVTVPLRDSHVRQPKFRSPKSRRR--P 250
Query: 133 AIVTFRFCFCFLC 145
A FR+C C LC
Sbjct: 251 ATTGFRWCLCLLC 289
>BM086669
Length = 415
Score = 28.1 bits (61), Expect = 1.6
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -3
Query: 123 PELNIRQVSSAIVTFRFCFCFLCFAFS 149
P N++ V + FRF FC LCF S
Sbjct: 407 PNPNMQSVHERSIFFRFKFC*LCFTLS 327
>TC222342 homologue to UP|Q93W01 (Q93W01) At2g01080/F23H14.5, partial (19%)
Length = 354
Score = 28.1 bits (61), Expect = 1.6
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +3
Query: 110 AIKDNYQKHYKFNPELNIRQVSSAIVTFRFCFCFLCFAFSF 150
A+ NY + +++P R SSA +F+ C C L FSF
Sbjct: 165 AVAANYNGYRQYHPTTPARSSSSA--SFKGCCCCLFLLFSF 281
>CO980352
Length = 639
Score = 27.7 bits (60), Expect = 2.1
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -2
Query: 131 SSAIVTFRFCFCFLCF 146
+S I F FCFCF CF
Sbjct: 158 NSPIFLFCFCFCFFCF 111
>TC227624
Length = 808
Score = 26.9 bits (58), Expect = 3.6
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +2
Query: 110 AIKDNYQKHYKFNPELNIRQV 130
AIK+NY + KF EL RQV
Sbjct: 2 AIKENYNEKNKFKTELTARQV 64
>BG550714 similar to GP|18146784|db PBng110 {Vigna radiata}, partial (69%)
Length = 413
Score = 26.6 bits (57), Expect = 4.8
Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 4/83 (4%)
Frame = +3
Query: 52 MHLFLFDTDVKLL----YGIYLATSTGMLNIEPYAFGGRFPAQVSFKILKDCLPLPENDF 107
+++F F+ VKLL Y LA S Y F G+ +S +L+ L + D+
Sbjct: 189 LYVFSFNQTVKLLKNSPYYCILAYS--------YVFRGKLKEDISTYVLQPILRVKNRDY 344
Query: 108 KHAIKDNYQKHYKFNPELNIRQV 130
K I+ ++ ++ E+ + V
Sbjct: 345 KQLIRKKLKELQEWIVEMYLDYV 413
>TC212194 similar to UP|Q9LK97 (Q9LK97) Exonuclease-like protein, partial
(68%)
Length = 1015
Score = 26.6 bits (57), Expect = 4.8
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = -2
Query: 131 SSAIVTFRFCFCFLCFAFS 149
+S+ F FCF FLCF F+
Sbjct: 813 TSSFFCFFFCFDFLCFCFN 757
>TC213877
Length = 677
Score = 26.6 bits (57), Expect = 4.8
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +3
Query: 128 RQVSSAIVTFRFCFCFLCF 146
+Q+SS+ FCFCF C+
Sbjct: 438 QQISSSYNHMPFCFCFTCY 494
>CO980758
Length = 874
Score = 26.6 bits (57), Expect = 4.8
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = +2
Query: 131 SSAIVTFRFCFCFLCFAFS 149
+S+ F FCF FLCF F+
Sbjct: 62 TSSFFCFFFCFDFLCFCFN 118
>BI317616
Length = 316
Score = 26.6 bits (57), Expect = 4.8
Identities = 15/39 (38%), Positives = 22/39 (55%)
Frame = -1
Query: 108 KHAIKDNYQKHYKFNPELNIRQVSSAIVTFRFCFCFLCF 146
+H ++D Y+K Y L++ +VSS I R FLCF
Sbjct: 118 RHEMEDGYRK*Y-----LSLAKVSSHIFGSRLQASFLCF 17
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.331 0.146 0.464
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,007,992
Number of Sequences: 63676
Number of extensions: 115641
Number of successful extensions: 1086
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 1061
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1081
length of query: 150
length of database: 12,639,632
effective HSP length: 89
effective length of query: 61
effective length of database: 6,972,468
effective search space: 425320548
effective search space used: 425320548
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC139708.3