BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139708.14 - phase: 0
(992 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC206300 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain... 469 e-132
TC218956 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain... 384 e-106
TC211906 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain... 185 5e-70
TC223212 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain... 255 8e-68
BM308817 similar to GP|13111324|db 110 kDa 4SNc-Tudor domain pro... 254 1e-67
TC218957 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain... 240 3e-63
TC235122 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain... 117 1e-41
AW756012 similar to GP|13111324|db 110 kDa 4SNc-Tudor domain pro... 161 1e-39
BM519682 similar to GP|10176871|dbj transcription factor-like pr... 146 5e-35
CA938357 similar to GP|18086332|gb| AT5g07350/T2I1_60 {Arabidops... 109 1e-29
TC210185 similar to UP|Q6TM09 (Q6TM09) Phospholipase C (Fragment... 31 2.4
TC203370 weakly similar to UP|Q9LX02 (Q9LX02) ESTs AU082316(E336... 29 9.0
BE803848 29 9.0
>TC206300 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain protein,
partial (26%)
Length = 1165
Score = 469 bits (1206), Expect = e-132
Identities = 232/259 (89%), Positives = 243/259 (93%)
Frame = +1
Query: 732 QQEVLKVTVTEVLGGGKFYVQTVGDQKIASIQNQLASLNLKDAPVIGAFNPKKGDIVLCY 791
QQEVLKV VTEVLGGGKFYVQTVGDQKIASIQ QLASLNLKDAPV+GAFNPKKGDIVLCY
Sbjct: 1 QQEVLKVIVTEVLGGGKFYVQTVGDQKIASIQQQLASLNLKDAPVLGAFNPKKGDIVLCY 180
Query: 792 FHADSSWYRAMVVNTPRGPVESSKDAFEVFYIDYGNQEVVPYSQLRPLDPSVSAAPGLAQ 851
FHAD SWYRAMVVNTPRGPVES D FEVFY+DYGNQEVVPYSQLRP+DPSVSAAPGLAQ
Sbjct: 181 FHADKSWYRAMVVNTPRGPVESPNDLFEVFYVDYGNQEVVPYSQLRPVDPSVSAAPGLAQ 360
Query: 852 LCSLAYIKLPNLEEDFGQEAAEYLSELTLSSGKEFRAMVEEKDTTGGKVKGQGTGPIIAV 911
LCSLAYIK+PNLEEDFGQEAAEYLSELTL+SGKEFRA VEEKDT+GGKVKGQGTG I+AV
Sbjct: 361 LCSLAYIKIPNLEEDFGQEAAEYLSELTLNSGKEFRAKVEEKDTSGGKVKGQGTGAILAV 540
Query: 912 TLVAVDSEISVNAAMLQEGLARMEKRNRWDRTARKQALDNLEMFQGEARTARRGMWQYGD 971
TLVAVD+EISVNAAMLQEGLAR EKRNRWDR R+ ALDNLE FQ EA+T+RRGMWQYGD
Sbjct: 541 TLVAVDAEISVNAAMLQEGLARTEKRNRWDRKDRQTALDNLENFQDEAKTSRRGMWQYGD 720
Query: 972 IQSDDEDTAPPQRKAGGGR 990
IQSDDEDTAPP RK GGGR
Sbjct: 721 IQSDDEDTAPPPRKTGGGR 777
>TC218956 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain protein,
partial (22%)
Length = 889
Score = 384 bits (986), Expect = e-106
Identities = 185/220 (84%), Positives = 203/220 (92%)
Frame = +3
Query: 769 LNLKDAPVIGAFNPKKGDIVLCYFHADSSWYRAMVVNTPRGPVESSKDAFEVFYIDYGNQ 828
LNL++AP++GAFNPKKGD VLC F AD SWYRAMVVN PRGPVES D FEVFYIDYGNQ
Sbjct: 9 LNLQEAPLLGAFNPKKGDTVLCLFGADKSWYRAMVVNGPRGPVESPNDMFEVFYIDYGNQ 188
Query: 829 EVVPYSQLRPLDPSVSAAPGLAQLCSLAYIKLPNLEEDFGQEAAEYLSELTLSSGKEFRA 888
E VPYSQLRP+DPSVSAAPG+AQLCSLAY+K+PNLEEDFG+EAAEYLSELTL+SGKEFRA
Sbjct: 189 EEVPYSQLRPIDPSVSAAPGIAQLCSLAYVKVPNLEEDFGEEAAEYLSELTLNSGKEFRA 368
Query: 889 MVEEKDTTGGKVKGQGTGPIIAVTLVAVDSEISVNAAMLQEGLARMEKRNRWDRTARKQA 948
VEE+DT+GGK KGQGTGP++AVTLVAVDS+ISVNAAMLQEGLAR+EKRNRWDR R+QA
Sbjct: 369 KVEERDTSGGKAKGQGTGPVLAVTLVAVDSDISVNAAMLQEGLARLEKRNRWDRKERQQA 548
Query: 949 LDNLEMFQGEARTARRGMWQYGDIQSDDEDTAPPQRKAGG 988
LDNL+ FQGEART R GMWQYGDIQSDDEDTAPP RKAGG
Sbjct: 549 LDNLDPFQGEARTNRCGMWQYGDIQSDDEDTAPPARKAGG 668
>TC211906 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain protein,
partial (15%)
Length = 536
Score = 185 bits (470), Expect(2) = 5e-70
Identities = 96/119 (80%), Positives = 103/119 (85%), Gaps = 1/119 (0%)
Frame = +1
Query: 466 DGSAVPPGAVDSRVMDFGSVFVLSSGKADGDDAPSPAVPA-SQQTGLNVAELIIGRGFGT 524
DGS VP A DS VMDFGSVF+LS K D DDAPS A PA SQQ G+NVAELI+GRGFGT
Sbjct: 4 DGSVVP-SAADS*VMDFGSVFLLSGAKVDNDDAPSSAPPAGSQQNGVNVAELIVGRGFGT 180
Query: 525 VIRHRDFEERSNFYDALLAAEARAISGRKGIHSAKDPPVMHITDLITASAKKAKDFLPF 583
VIRHRDFEERSN+YD+LLAAE+RAISGRKG HSAKDPPVMHITDL ASAKKA+DFLPF
Sbjct: 181 VIRHRDFEERSNYYDSLLAAESRAISGRKGTHSAKDPPVMHITDLTMASAKKARDFLPF 357
Score = 99.0 bits (245), Expect(2) = 5e-70
Identities = 49/68 (72%), Positives = 55/68 (80%), Gaps = 1/68 (1%)
Frame = +2
Query: 575 KKAKDFLPFLHRS-RRVPAVVEYVFSGHRFKLLIPKETCSIAFAFSGVRCPGREEPYSDE 633
+K + FLH + AVVEYV GHRFKLLIPKETCSIAF+FSGVRCPGR+EPYSDE
Sbjct: 332 RKPETSCXFLHXK*KEFRAVVEYVLRGHRFKLLIPKETCSIAFSFSGVRCPGRDEPYSDE 511
Query: 634 AIALMRRR 641
AIALMRR+
Sbjct: 512 AIALMRRK 535
>TC223212 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain protein,
partial (13%)
Length = 431
Score = 255 bits (651), Expect = 8e-68
Identities = 124/143 (86%), Positives = 132/143 (91%)
Frame = +3
Query: 339 NMMEDEAKKKLKAAELEAKKTRLRIWTNYVPPTSNSKAIHDQNFTGKVVEVVSGDCVIVA 398
NMME+EAK+KLK AEL+AKK RLR+WTNYVPP SNSKAIH+QNF+GKVVEVVSGDC++VA
Sbjct: 3 NMMEEEAKRKLKTAELQAKKDRLRMWTNYVPPPSNSKAIHNQNFSGKVVEVVSGDCIVVA 182
Query: 399 DDSIPYGSPQAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRTRLIGRQVNVQMEY 458
DDSIPYGSP AERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRT LIGRQVNVQMEY
Sbjct: 183 DDSIPYGSPLAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRTHLIGRQVNVQMEY 362
Query: 459 SRKVGPVDGSAVPPGAVDSRVMD 481
SRKV P DGS VP A DSRVMD
Sbjct: 363 SRKVSPTDGSVVPSTASDSRVMD 431
Score = 49.7 bits (117), Expect = 6e-06
Identities = 30/81 (37%), Positives = 48/81 (59%), Gaps = 4/81 (4%)
Frame = +3
Query: 12 YKAKVKAVPSGDCIVVV--SVAANAKLGVLPEKSITLSSLIAPRLA--RRGGVDEPFAWE 67
+ KV V SGDCIVV S+ + L E+ + LSS+ P++ RR P+A E
Sbjct: 132 FSGKVVEVVSGDCIVVADDSIPYGSPLA---ERRVNLSSIRCPKMGNPRRDEKPAPYARE 302
Query: 68 SREFLRKLLIGKEITFRIDYT 88
++EFLR LIG+++ +++Y+
Sbjct: 303 AKEFLRTHLIGRQVNVQMEYS 365
>BM308817 similar to GP|13111324|db 110 kDa 4SNc-Tudor domain protein {Pisum
sativum}, partial (19%)
Length = 428
Score = 254 bits (649), Expect = 1e-67
Identities = 122/141 (86%), Positives = 132/141 (93%)
Frame = +2
Query: 347 KKLKAAELEAKKTRLRIWTNYVPPTSNSKAIHDQNFTGKVVEVVSGDCVIVADDSIPYGS 406
+KLK +EL+AKK RL+IWTNYVPP +NSKAIHDQNFTGKVVEVVSGDC+IVADD IPYGS
Sbjct: 2 RKLKTSELQAKKNRLKIWTNYVPPATNSKAIHDQNFTGKVVEVVSGDCIIVADDLIPYGS 181
Query: 407 PQAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRTRLIGRQVNVQMEYSRKVGPVD 466
P AERRVNLSSIRCPK+GNPRRDEKPAPYAREAKEFLRTRLIGRQVNVQMEYSRKVGP D
Sbjct: 182 PLAERRVNLSSIRCPKVGNPRRDEKPAPYAREAKEFLRTRLIGRQVNVQMEYSRKVGPAD 361
Query: 467 GSAVPPGAVDSRVMDFGSVFV 487
GSAVP GA ++R MDFGSVF+
Sbjct: 362 GSAVPSGASEARAMDFGSVFL 424
Score = 48.9 bits (115), Expect = 1e-05
Identities = 37/107 (34%), Positives = 55/107 (50%), Gaps = 17/107 (15%)
Frame = +2
Query: 12 YKAKVKAVPSGDCIVVVSVAANAKLGVLPEKSITLSSLIAPRLA--RRGGVDEPFAWESR 69
+ KV V SGDCI+V L E+ + LSS+ P++ RR P+A E++
Sbjct: 107 FTGKVVEVVSGDCIIVADDLIPYG-SPLAERRVNLSSIRCPKVGNPRRDEKPAPYAREAK 283
Query: 70 EFLRKLLIGKEITFRIDYT----------VPSINRE-----FGTVFL 101
EFLR LIG+++ +++Y+ VPS E FG+VFL
Sbjct: 284 EFLRTRLIGRQVNVQMEYSRKVGPADGSAVPSGASEARAMDFGSVFL 424
Score = 30.0 bits (66), Expect = 5.3
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +2
Query: 698 LEQAEQSAKSKKLKIWENYVEGEVVPSGANVESKQQEVLKVTVTEVLGG 746
L+ +E AK +LKIW NY VP N ++ + V EV+ G
Sbjct: 8 LKTSELQAKKNRLKIWTNY-----VPPATNSKAIHDQNFTGKVVEVVSG 139
>TC218957 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain protein,
partial (14%)
Length = 629
Score = 240 bits (612), Expect = 3e-63
Identities = 117/136 (86%), Positives = 127/136 (93%)
Frame = +2
Query: 853 CSLAYIKLPNLEEDFGQEAAEYLSELTLSSGKEFRAMVEEKDTTGGKVKGQGTGPIIAVT 912
CSLAY+K+PNLEEDFGQEAAEYLSELTL+SGKEFRA VEE+DT+GGK KGQGTG ++AVT
Sbjct: 2 CSLAYVKVPNLEEDFGQEAAEYLSELTLNSGKEFRAKVEERDTSGGKAKGQGTGTVLAVT 181
Query: 913 LVAVDSEISVNAAMLQEGLARMEKRNRWDRTARKQALDNLEMFQGEARTARRGMWQYGDI 972
LVAVDSEISVNAAMLQEGLAR+EKRNRWD R+QALDNL FQGEART+RRGMWQYGDI
Sbjct: 182 LVAVDSEISVNAAMLQEGLARLEKRNRWDGKERQQALDNLVPFQGEARTSRRGMWQYGDI 361
Query: 973 QSDDEDTAPPQRKAGG 988
QSDDEDTAPP RKAGG
Sbjct: 362 QSDDEDTAPPARKAGG 409
>TC235122 similar to UP|Q8L5C2 (Q8L5C2) 110 kDa 4SNc-Tudor domain protein,
partial (11%)
Length = 599
Score = 117 bits (293), Expect(2) = 1e-41
Identities = 61/73 (83%), Positives = 64/73 (87%)
Frame = +3
Query: 221 QAPQMGRRAAPESVVVPEVTVDTTNGDVPAEPRAPLTSAQRLAVSASAAETSADPFGADA 280
QAPQMGRRAAPESVV PE+ D TNGDVP EP+APLTSAQRLAVS S AET+ADPF DA
Sbjct: 81 QAPQMGRRAAPESVVEPELVSDDTNGDVPGEPQAPLTSAQRLAVSTS-AETAADPFAHDA 257
Query: 281 KFFTEMRVLNRDV 293
KFFTEMRVLNRDV
Sbjct: 258 KFFTEMRVLNRDV 296
Score = 72.4 bits (176), Expect(2) = 1e-41
Identities = 35/36 (97%), Positives = 36/36 (99%)
Frame = +2
Query: 293 VRIVLEGVDKFSNLIGSVYYPDGESAKDLALELVEN 328
VR+VLEGVDKFSNLIGSVYYPDGESAKDLALELVEN
Sbjct: 365 VRLVLEGVDKFSNLIGSVYYPDGESAKDLALELVEN 472
>AW756012 similar to GP|13111324|db 110 kDa 4SNc-Tudor domain protein {Pisum
sativum}, partial (15%)
Length = 319
Score = 161 bits (408), Expect = 1e-39
Identities = 79/106 (74%), Positives = 88/106 (82%)
Frame = +2
Query: 511 LNVAELIIGRGFGTVIRHRDFEERSNFYDALLAAEARAISGRKGIHSAKDPPVMHITDLI 570
+NV ELI+ RGFGTVIRHRDFEERSN+YDALL AE+ AISG+KGIHSAKD P MHITDL
Sbjct: 2 VNVGELIVSRGFGTVIRHRDFEERSNYYDALLTAESLAISGKKGIHSAKDSPSMHITDLT 181
Query: 571 TASAKKAKDFLPFLHRSRRVPAVVEYVFSGHRFKLLIPKETCSIAF 616
T+SAKKAKD L FL+RS +PA VEY GHRFKLL+P ETC I F
Sbjct: 182 TSSAKKAKDSLSFLYRST*IPARVEYALGGHRFKLLVPDETCIIVF 319
>BM519682 similar to GP|10176871|dbj transcription factor-like protein
{Arabidopsis thaliana}, partial (7%)
Length = 434
Score = 146 bits (368), Expect = 5e-35
Identities = 69/95 (72%), Positives = 85/95 (88%)
Frame = +3
Query: 1 MAATAAGNSAWYKAKVKAVPSGDCIVVVSVAANAKLGVLPEKSITLSSLIAPRLARRGGV 60
MA+ A+G + WY+ +VKAVPSGDC+V+V++++ K G LPEK+ITLSSLIAPRLARRGGV
Sbjct: 153 MASAASGATGWYRGRVKAVPSGDCLVIVAISST-KPGPLPEKTITLSSLIAPRLARRGGV 329
Query: 61 DEPFAWESREFLRKLLIGKEITFRIDYTVPSINRE 95
DEPFAWESREFLRKL IGKE+TFR+DY VPSI+R+
Sbjct: 330 DEPFAWESREFLRKLCIGKEVTFRVDYNVPSISRD 434
Score = 53.1 bits (126), Expect = 6e-07
Identities = 27/78 (34%), Positives = 46/78 (58%)
Frame = +3
Query: 382 FTGKVVEVVSGDCVIVADDSIPYGSPQAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKE 441
+ G+V V SGDC+++ S P E+ + LSS+ P++ RR P+A E++E
Sbjct: 186 YRGRVKAVPSGDCLVIVAISSTKPGPLPEKTITLSSLIAPRLA--RRGGVDEPFAWESRE 359
Query: 442 FLRTRLIGRQVNVQMEYS 459
FLR IG++V +++Y+
Sbjct: 360 FLRKLCIGKEVTFRVDYN 413
>CA938357 similar to GP|18086332|gb| AT5g07350/T2I1_60 {Arabidopsis
thaliana}, partial (7%)
Length = 267
Score = 109 bits (272), Expect(2) = 1e-29
Identities = 54/61 (88%), Positives = 57/61 (92%)
Frame = +3
Query: 646 DVEIEVETVDRTGTFLGSLWESRANGAVPLLEAGLAKLQTSFGSDRIPDLHVLEQAEQSA 705
DVEIEVETVDRTGTFLGSLWESR N A+ LLEAGLAKLQTSFGSDRIPD H+L+QAEQSA
Sbjct: 3 DVEIEVETVDRTGTFLGSLWESRTNVAITLLEAGLAKLQTSFGSDRIPDFHLLDQAEQSA 182
Query: 706 K 706
K
Sbjct: 183 K 185
Score = 40.4 bits (93), Expect(2) = 1e-29
Identities = 18/24 (75%), Positives = 21/24 (87%)
Frame = +2
Query: 710 LKIWENYVEGEVVPSGANVESKQQ 733
LKIWEN+VEGE V +GA VE+KQQ
Sbjct: 194 LKIWENFVEGEEVSNGAAVENKQQ 265
>TC210185 similar to UP|Q6TM09 (Q6TM09) Phospholipase C (Fragment), partial
(76%)
Length = 1184
Score = 31.2 bits (69), Expect = 2.4
Identities = 18/62 (29%), Positives = 34/62 (54%)
Frame = +3
Query: 228 RAAPESVVVPEVTVDTTNGDVPAEPRAPLTSAQRLAVSASAAETSADPFGADAKFFTEMR 287
++APE + + GD+ E +A + +RL++S A E +++ +GAD FT+
Sbjct: 240 KSAPEYKHLITIHAGKPKGDIQDELKAA-GAVRRLSLSEQALEKASESYGADIVRFTQNN 416
Query: 288 VL 289
+L
Sbjct: 417 IL 422
>TC203370 weakly similar to UP|Q9LX02 (Q9LX02) ESTs AU082316(E3368), partial
(27%)
Length = 1507
Score = 29.3 bits (64), Expect = 9.0
Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 2/41 (4%)
Frame = +2
Query: 327 ENGFAKYVEWSANMMEDEAKKKLKAAELEAK--KTRLRIWT 365
+NGF + +W A + E+E KKK K + + K K + WT
Sbjct: 380 KNGFDRKYKWVAEVAEEEDKKKKKNKKKQVKVLKDKSARWT 502
>BE803848
Length = 400
Score = 29.3 bits (64), Expect = 9.0
Identities = 16/53 (30%), Positives = 26/53 (48%)
Frame = +1
Query: 21 SGDCIVVVSVAANAKLGVLPEKSITLSSLIAPRLARRGGVDEPFAWESREFLR 73
S C + S + + K + ++S++ +A GGV P W+SR FLR
Sbjct: 151 SSSCDITESEQCRDESAAMVLKFVAVASIL---VAGFGGVSIPLVWKSRRFLR 300
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.133 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,359,618
Number of Sequences: 63676
Number of extensions: 422827
Number of successful extensions: 1933
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1926
length of query: 992
length of database: 12,639,632
effective HSP length: 106
effective length of query: 886
effective length of database: 5,889,976
effective search space: 5218518736
effective search space used: 5218518736
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 64 (29.3 bits)
Medicago: description of AC139708.14