BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138580.8 - phase: 0
(268 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC210077 156 1e-38
TC222440 similar to UP|Q6K9R7 (Q6K9R7) DNA ligase-like, partial ... 100 1e-21
BM270760 58 5e-09
TC206748 similar to UP|Q9ASY7 (Q9ASY7) At2g26200/T1D16.16, parti... 56 1e-08
TC212602 48 5e-06
TC230758 similar to UP|Q9FGZ0 (Q9FGZ0) Arabidopsis thaliana geno... 48 5e-06
BE347716 42 4e-04
TC208871 similar to UP|O22847 (O22847) Expressed protein (At2g43... 41 5e-04
TC233834 similar to UP|Q9FGZ0 (Q9FGZ0) Arabidopsis thaliana geno... 39 0.003
AI437737 37 0.009
TC213786 similar to UP|Q756U1 (Q756U1) AER161Cp, partial (6%) 35 0.027
TC218169 similar to UP|Q84TF1 (Q84TF1) At4g28830, partial (94%) 32 0.39
BE556459 32 0.39
TC214510 30 1.5
AW185542 similar to GP|12584943|gb| phosphoethanolamine N-methyl... 29 1.9
BU762005 similar to GP|9294061|dbj WD40-repeat protein {Arabidop... 28 3.3
TC226080 similar to UP|Q7XA63 (Q7XA63) At5g19860, partial (62%) 28 3.3
BG510625 28 4.3
BU763225 28 4.3
TC207587 weakly similar to UP|Q9FKY4 (Q9FKY4) Gb|AAD32907.1, par... 28 5.6
>TC210077
Length = 581
Score = 156 bits (394), Expect = 1e-38
Identities = 75/86 (87%), Positives = 83/86 (96%)
Frame = +2
Query: 183 DPTPDYILGSDVVYSEGAVVDLLETLGQLSGPNTTIFLAGELRNDAILEYFLEAAMNDFT 242
+P PD+++GSDVVYSEGAVVDLLETL QLSGPNTTIFLAGELRNDAILEYFLEAAM++FT
Sbjct: 5 EPKPDFVIGSDVVYSEGAVVDLLETLMQLSGPNTTIFLAGELRNDAILEYFLEAAMDNFT 184
Query: 243 IGRVDQTLWHPDYHSNRVVLYVLVKK 268
IGRV+QTLWHPDY SNRVV+YVLVKK
Sbjct: 185 IGRVEQTLWHPDYCSNRVVIYVLVKK 262
>TC222440 similar to UP|Q6K9R7 (Q6K9R7) DNA ligase-like, partial (30%)
Length = 644
Score = 99.8 bits (247), Expect = 1e-21
Identities = 55/156 (35%), Positives = 89/156 (56%), Gaps = 8/156 (5%)
Frame = +3
Query: 47 LSKPNAFVAQSSLQLRLDSCGHSLSILQSPSSLGKPGVTGSVMWDSGIVLGKFLEHSVDS 106
L +P+ + ++ ++ L+ GH L P+S + +WD+ +V KFLE +
Sbjct: 189 LMEPDRLNSPTTFEMPLEVMGHELQFSHDPNSKH----LXTTVWDASLVFAKFLERNCRK 356
Query: 107 GMLV---LQGKKIVELGSGCGLVGCIAALLGGEVILTDLPDRMRLLRKNIETNMKHISLR 163
G L+GK+++ELG+GCG+ G ALLG +VI+TD + + LL++N+E N+ I +
Sbjct: 357 GRFSPAKLKGKRVIELGAGCGVSGIGMALLGCDVIVTDQKEVLPLLQRNVERNISRIMQK 536
Query: 164 -----GSITATELTWGDDPDQELIDPTPDYILGSDV 194
GSI EL WGD+ + + P DYI+G+DV
Sbjct: 537 NPDSFGSIKVAELQWGDESHIKAVGPPFDYIIGTDV 644
>BM270760
Length = 421
Score = 57.8 bits (138), Expect = 5e-09
Identities = 29/60 (48%), Positives = 37/60 (61%)
Frame = +3
Query: 164 GSITATELTWGDDPDQELIDPTPDYILGSDVVYSEGAVVDLLETLGQLSGPNTTIFLAGE 223
GSI EL WGD+ + + P DYI+G+DVVY E + LL+T+ LSGP TT L E
Sbjct: 240 GSIKVAELQWGDESHIKAVGPPFDYIIGTDVVYVEHLLEPLLQTILALSGPRTTTMLGYE 419
>TC206748 similar to UP|Q9ASY7 (Q9ASY7) At2g26200/T1D16.16, partial (57%)
Length = 1003
Score = 56.2 bits (134), Expect = 1e-08
Identities = 38/137 (27%), Positives = 70/137 (50%), Gaps = 7/137 (5%)
Frame = +1
Query: 85 TGSVMWDSGIVLGKFLEHSVDSGMLVLQGKKIVELGSGCGLVGCIAALLGGEVILTDLPD 144
TG ++W+S ++ L + + ++ GK+++ELG G G + + A+ ++++ D
Sbjct: 202 TGLMLWESARLMASILAENPN----IVAGKRVLELGCGSGGICSMIAVRDADLVVATDGD 369
Query: 145 --RMRLLRKNIETNMKHISLRGSITATELTWGDDPDQELIDPTP-----DYILGSDVVYS 197
+ LL KN+ +N++ SL +T +L WG+ E I D I+G+DV Y
Sbjct: 370 GFALDLLTKNVASNIEP-SLLTKLTTKKLEWGNKEHIECIKEVVSNRGFDVIIGTDVTYI 546
Query: 198 EGAVVDLLETLGQLSGP 214
A++ L T +L P
Sbjct: 547 PDAILPLFATAKELIAP 597
>TC212602
Length = 452
Score = 47.8 bits (112), Expect = 5e-06
Identities = 24/72 (33%), Positives = 46/72 (63%), Gaps = 1/72 (1%)
Frame = +2
Query: 84 VTGSVMWDSGIVLGKFLEHSVDSGMLVLQGKKIVELGSGCGLVGCIAALLGGEVILTDLP 143
+TG ++W ++L ++L +V+ +LQG +ELGSG G+ G + + +V++TD
Sbjct: 221 LTGQLVWPGAMLLNEYLSKNVN----LLQGCTAIELGSGVGITGILCSRFCHKVVMTDHN 388
Query: 144 DR-MRLLRKNIE 154
+ +++L+KNIE
Sbjct: 389 EEVIKILKKNIE 424
>TC230758 similar to UP|Q9FGZ0 (Q9FGZ0) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K6M13, partial (46%)
Length = 678
Score = 47.8 bits (112), Expect = 5e-06
Identities = 39/114 (34%), Positives = 49/114 (42%), Gaps = 4/114 (3%)
Frame = +1
Query: 134 GGEVILTDLPDRMRLLRKNIETNMKHISLRGS-ITATELTWGDDPDQELIDPTPDYILGS 192
G V LTD P + LR N + N + G IT L WG D E I D +L S
Sbjct: 1 GAHVTLTDFPHVVPNLRFNADANAAVVGPTGGVITVAPLRWGHAADVEAIGREFDLVLAS 180
Query: 193 DVVYSEGAVVDLLETLG--QLSGPN-TTIFLAGELRNDAILEYFLEAAMNDFTI 243
DVVY + LLETL LS N +F+ +R F + A F +
Sbjct: 181 DVVYHDHLYEPLLETLRLMMLSERNGKMVFVMAHMRRWKKESAFFKKARKHFNV 342
>BE347716
Length = 473
Score = 41.6 bits (96), Expect = 4e-04
Identities = 33/106 (31%), Positives = 46/106 (43%)
Frame = +2
Query: 86 GSVMWDSGIVLGKFLEHSVDSGMLVLQGKKIVELGSGCGLVGCIAALLGGEVILTDLPDR 145
G +W +VL +++ G +VELG+G L G +AA LG V LTD R
Sbjct: 209 GLFVWPCSVVLAEYIWQHKHR----FSGATVVELGAGTSLPGLVAAKLGARVTLTDDSTR 376
Query: 146 MRLLRKNIETNMKHISLRGSITATELTWGDDPDQELIDPTPDYILG 191
+ +L NM+ + LTWG D + P ILG
Sbjct: 377 LEVL-----DNMRRV--------LGLTWG-VWDSSIFSLQPTIILG 472
>TC208871 similar to UP|O22847 (O22847) Expressed protein
(At2g43320/T1O24.6), partial (42%)
Length = 685
Score = 41.2 bits (95), Expect = 5e-04
Identities = 19/49 (38%), Positives = 26/49 (52%)
Frame = +2
Query: 90 WDSGIVLGKFLEHSVDSGMLVLQGKKIVELGSGCGLVGCIAALLGGEVI 138
W S I L L+H + G L +GK+++EL GL G A L G V+
Sbjct: 386 WTSSIDLVSVLKHEIRDGQLTFRGKRVLELSCNYGLPGIFACLKGASVV 532
>TC233834 similar to UP|Q9FGZ0 (Q9FGZ0) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K6M13, partial (18%)
Length = 432
Score = 38.5 bits (88), Expect = 0.003
Identities = 20/31 (64%), Positives = 23/31 (73%), Gaps = 1/31 (3%)
Frame = +2
Query: 114 KKIVELGSGCGLVG-CIAALLGGEVILTDLP 143
++I+ELGSG GLVG AA LG V LTDLP
Sbjct: 332 RRIIELGSGTGLVGIAAAATLGAHVTLTDLP 424
>AI437737
Length = 282
Score = 37.0 bits (84), Expect = 0.009
Identities = 19/49 (38%), Positives = 29/49 (58%), Gaps = 7/49 (14%)
Frame = +1
Query: 86 GSVMWDSGIVLGKFLE-------HSVDSGMLVLQGKKIVELGSGCGLVG 127
G+ +W +VL KF E ++ + +L QGK+ VELG+GCG+ G
Sbjct: 136 GTTVWPCSLVLVKFAERWGPPSDNNPYARVLDFQGKRAVELGTGCGVAG 282
>TC213786 similar to UP|Q756U1 (Q756U1) AER161Cp, partial (6%)
Length = 446
Score = 35.4 bits (80), Expect = 0.027
Identities = 25/75 (33%), Positives = 39/75 (51%), Gaps = 1/75 (1%)
Frame = +1
Query: 68 HSLSI-LQSPSSLGKPGVTGSVMWDSGIVLGKFLEHSVDSGMLVLQGKKIVELGSGCGLV 126
HS S+ +Q + P V G +W + ++L F+ H S + Q + +LG+G GLV
Sbjct: 205 HSFSVRIQHNITSSIPNV-GLQVWRAELLLSDFILHKASSSSQLHQVIAL-QLGAGTGLV 378
Query: 127 GCIAALLGGEVILTD 141
G + A + V LTD
Sbjct: 379 GLLLARVANAVFLTD 423
>TC218169 similar to UP|Q84TF1 (Q84TF1) At4g28830, partial (94%)
Length = 891
Score = 31.6 bits (70), Expect = 0.39
Identities = 21/68 (30%), Positives = 33/68 (47%), Gaps = 12/68 (17%)
Frame = +2
Query: 114 KKIVELGSGCGLVGCIAALLGGEVIL-TDL-PDRMRLLRKN----------IETNMKHIS 161
K + + G GCG +G AALL E +L D+ P+ + + N I++N+ +
Sbjct: 182 KVVADFGCGCGTLGVAAALLSAEHVLGIDIDPESLEIASNNAEELEVDIDFIQSNVMDLG 361
Query: 162 LRGSITAT 169
RG I T
Sbjct: 362 WRGRIVDT 385
>BE556459
Length = 250
Score = 31.6 bits (70), Expect = 0.39
Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 4/55 (7%)
Frame = +2
Query: 161 SLRGSITATELTWGD-DPDQELIDPTP---DYILGSDVVYSEGAVVDLLETLGQL 211
S+ + A +L WG+ D E++ P D+ILG+D+ + + ++ L +++ QL
Sbjct: 26 SISHGLVAEKLEWGNTDQINEILQKHPGGFDFILGADICFQQSSIPMLFDSVKQL 190
>TC214510
Length = 994
Score = 29.6 bits (65), Expect = 1.5
Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 4/65 (6%)
Frame = +1
Query: 155 TNMKHISLRGSITATELTWGDDPDQELIDP---TPDYILGSDVV-YSEGAVVDLLETLGQ 210
TN+ H+SL G + +L WG Q P TP + ++++ S LL+ L
Sbjct: 211 TNLTHVSLNGCVNMHDLNWGSSRGQSDNFPAVNTPSWASSNEIISESSEHSARLLQNLYC 390
Query: 211 LSGPN 215
+ PN
Sbjct: 391 VGCPN 405
>AW185542 similar to GP|12584943|gb| phosphoethanolamine N-methyltransferase
{Lycopersicon esculentum}, partial (18%)
Length = 294
Score = 29.3 bits (64), Expect = 1.9
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = +1
Query: 112 QGKKIVELGSGCGLVGCIAALLGGEVILTDLPDRMRLLRKNIETNMKH 159
+GK ++ELG+G G AL G+++ D D ++KN N H
Sbjct: 100 EGKSVIELGAGIGRFTGELALKAGQLLAVDFID--TAIKKNETINGHH 237
>BU762005 similar to GP|9294061|dbj WD40-repeat protein {Arabidopsis
thaliana}, partial (24%)
Length = 466
Score = 28.5 bits (62), Expect = 3.3
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Frame = +1
Query: 33 AEETMLLWGIQQPTLSKPNAFVAQSSLQLRLDSCGHS---LSILQSPSSLGKPGVTGSVM 89
A T+ +WG + + K V L R+D S L I+ GK V + M
Sbjct: 25 ASGTVRIWGTRNDFVLKKEFRV----LSARIDDLQWSPDGLRIVACGEGKGKSFVR-AFM 189
Query: 90 WDSGIVLGKFLEHS 103
WDSG +G+F HS
Sbjct: 190 WDSGTNVGEFFGHS 231
>TC226080 similar to UP|Q7XA63 (Q7XA63) At5g19860, partial (62%)
Length = 877
Score = 28.5 bits (62), Expect = 3.3
Identities = 16/68 (23%), Positives = 32/68 (46%)
Frame = -2
Query: 173 WGDDPDQELIDPTPDYILGSDVVYSEGAVVDLLETLGQLSGPNTTIFLAGELRNDAILEY 232
W DPD ++P + + + + +GA+ +L L + +++AG +ND L
Sbjct: 426 WQIDPDLVDVEPDEEALDLNALEIGDGAIAELAGDLALVVNQVIELYVAGLRQNDDELAI 247
Query: 233 FLEAAMND 240
+E + D
Sbjct: 246 LVEFVVGD 223
>BG510625
Length = 440
Score = 28.1 bits (61), Expect = 4.3
Identities = 10/35 (28%), Positives = 21/35 (59%)
Frame = +1
Query: 86 GSVMWDSGIVLGKFLEHSVDSGMLVLQGKKIVELG 120
G +W+ + L K L + +G++ GK+++E+G
Sbjct: 322 GLKLWEGSLDLIKALRSDIKNGLISFAGKRVLEVG 426
>BU763225
Length = 426
Score = 28.1 bits (61), Expect = 4.3
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +2
Query: 113 GKKIVELGSGCGLVGCIAALLGGEVI 138
GK+++E+GSG GL G A L G +
Sbjct: 11 GKRVLEVGSGHGLPGIFALLEGAAAV 88
>TC207587 weakly similar to UP|Q9FKY4 (Q9FKY4) Gb|AAD32907.1, partial (34%)
Length = 1065
Score = 27.7 bits (60), Expect = 5.6
Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
Frame = +1
Query: 56 QSSLQLRLDSCGHSLSILQSPSSLGK--PGVTGSVMWDSGIVLGKFLEHSVDSGMLVLQG 113
+SS ++ H L+ L SP ++ + G+V W I++G D+ + V+Q
Sbjct: 76 ESSWHRMVEVMSHLLAELMSPPAIATFFGFLFGAVAWLRNIIIGD------DAPLRVIQD 237
Query: 114 KKIVELGSGCGLVGCIAALLGGEV 137
+ G G + CI LLGG +
Sbjct: 238 SLQL---LGNGTIPCITLLLGGNL 300
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,783,090
Number of Sequences: 63676
Number of extensions: 136823
Number of successful extensions: 723
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 721
length of query: 268
length of database: 12,639,632
effective HSP length: 96
effective length of query: 172
effective length of database: 6,526,736
effective search space: 1122598592
effective search space used: 1122598592
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC138580.8