BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138580.5 + phase: 0 /pseudo
(448 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA783280 weakly similar to PIR|T01871|T01 RNA-directed DNA polym... 104 9e-23
BM891241 67 5e-16
TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {A... 80 3e-15
CF922212 79 3e-15
BE554941 74 1e-13
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 69 6e-12
BM891637 64 1e-10
CO982349 61 9e-10
BI972659 50 2e-06
BI427218 49 4e-06
TC231922 44 2e-04
CO978908 41 0.001
TC222557 40 0.002
BU090318 weakly similar to GP|9743345|gb| F21J9.30 {Arabidopsis ... 38 0.011
AI496436 35 0.053
CF920672 32 0.45
BM309783 32 0.58
BQ297454 32 0.76
BF596367 31 0.99
BM893063 31 1.3
>CA783280 weakly similar to PIR|T01871|T01 RNA-directed DNA polymerase
homolog T24M8.8 - Arabidopsis thaliana, partial (4%)
Length = 456
Score = 104 bits (259), Expect = 9e-23
Identities = 61/161 (37%), Positives = 86/161 (52%), Gaps = 5/161 (3%)
Frame = +2
Query: 207 SFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSLRKEYGGLGVTRLREFNVAL 266
SFF P II+ ++SL +F WG D RKI+ + W T+ L K GGLG+ LR FN L
Sbjct: 5 SFFSLP*KIIAKLESLQRRFLWGGEADSRKIAWVNWKTVCLPKAKGGLGIKDLRTFNTTL 184
Query: 267 LGKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSSWWREIVRI----RDG 322
LGKW W L ++ W KVL ++YG LE+G G S+WW+++++ R+
Sbjct: 185 LGKWRWDLFYIQQEPWAKVLQSKYG-GWRALEEG---SSGSKDSAWWKDLIKTQQLQRNI 352
Query: 323 ICEGGEGWFGACVRRRVGDGAKIDFWRDRWCG-DVPLCDRF 362
+ W +VG G +I FW D W D+ L D+F
Sbjct: 353 PLKRETIW-------KVGGGDRIKFWEDLWTNTDLSLRDKF 454
>BM891241
Length = 407
Score = 66.6 bits (161), Expect(2) = 5e-16
Identities = 26/63 (41%), Positives = 42/63 (66%)
Frame = -2
Query: 229 GEGEDKRKIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVA 288
G D +KI ++W+ + L K GGLG+ + +FN+ALLGKW W L D++ LW +++ +
Sbjct: 406 GGDHDHKKIPWVKWEVVCLPKTKGGLGIKDISKFNIALLGKWIWALASDQQQLWARIINS 227
Query: 289 RYG 291
+YG
Sbjct: 226 KYG 218
Score = 35.8 bits (81), Expect(2) = 5e-16
Identities = 19/58 (32%), Positives = 31/58 (52%), Gaps = 1/58 (1%)
Frame = -3
Query: 310 SSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWRDRWCG-DVPLCDRFSRLY 366
S WWR++ R + F + +VG G KI+FW D+W G D L ++++L+
Sbjct: 174 SYWWRDL---RKFYHQSDHSIFHQYMSWKVGCGDKINFWTDKWLGEDYTLEQKYNQLF 10
>TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {Arabidopsis
thaliana;} , partial (18%)
Length = 759
Score = 80.5 bits (197), Expect(2) = 3e-15
Identities = 37/93 (39%), Positives = 54/93 (57%)
Frame = +2
Query: 193 LLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSLRKEYG 252
L+KSVL+ALP+ LSFFK P I+ + +L +F WG + +I ++W + K G
Sbjct: 20 LIKSVLSALPIXXLSFFKIPQRIVDKLVTLQRQFLWGGTQHHNRIPWVKWADICNPKIDG 199
Query: 253 GLGVTRLREFNVALLGKWCWRLLLDREALWRKV 285
GLG+ L FN AL G+W W L + LW ++
Sbjct: 200 GLGIKDLSNFNAALRGRWIWGLASNHNQLWARL 298
Score = 19.2 bits (38), Expect(2) = 3e-15
Identities = 4/10 (40%), Positives = 9/10 (90%)
Frame = +3
Query: 310 SSWWREIVRI 319
SSWW+++ ++
Sbjct: 327 SSWWKDLKKL 356
>CF922212
Length = 445
Score = 79.3 bits (194), Expect = 3e-15
Identities = 51/142 (35%), Positives = 73/142 (50%), Gaps = 1/142 (0%)
Frame = -2
Query: 212 PSGIISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWC 271
P+ ++ + L F WG G ++KI+ + WD++ L KE GLG LR+FN ALLGK
Sbjct: 438 PNKVLDKLVRLQQWFLWGGGLGQKKIAWVNWDSVCLPKEKEGLGNRDLRKFNYALLGKRR 259
Query: 272 WRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSSWWREIVRIRDGICEGGEGWF 331
W L + L +VL ++Y + D E +S S WW+EI I +G WF
Sbjct: 258 WNLFHHQGELGARVLDSKY----KRWRNLDEERRVKSESFWWQEISFITHSTEDG--SWF 97
Query: 332 GACVRR-RVGDGAKIDFWRDRW 352
+ R+G AK+ FW D W
Sbjct: 96 EKRTKTGRLGCRAKVRFWEDGW 31
>BE554941
Length = 383
Score = 73.9 bits (180), Expect = 1e-13
Identities = 44/127 (34%), Positives = 66/127 (51%), Gaps = 2/127 (1%)
Frame = +1
Query: 255 GVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSC--SSW 312
G+ L FN++LL K L++ +A+W +L YG D L + G + S W
Sbjct: 7 GIKNLSIFNISLLVKRR*HFLVETKAIWAPILKFHYGSLD-------LSNTGSASRTSQW 165
Query: 313 WREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWRDRWCGDVPLCDRFSRLYDLAVNK 372
WR+I+RI G C+ GW + R++GD FWR R + PL +RL LA++K
Sbjct: 166 WRDILRI--GNCQYQPGWLTSTFCRKIGDRKDTLFWRHRCLNETPLMQSHARLXSLAMDK 339
Query: 373 SITVRNM 379
I+V+ M
Sbjct: 340 DISVQGM 360
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 68.6 bits (166), Expect = 6e-12
Identities = 29/62 (46%), Positives = 42/62 (66%)
Frame = +1
Query: 193 LLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSLRKEYG 252
L+KSVLT++P+Y SFF+ P ++ + + F WG G D++KIS IRW+ L L KE G
Sbjct: 724 LIKSVLTSIPIYFFSFFRVPQSVVDKLVKI*RTFLWGGGPDQKKISWIRWEKLCLPKERG 903
Query: 253 GL 254
G+
Sbjct: 904 GI 909
>BM891637
Length = 427
Score = 64.3 bits (155), Expect = 1e-10
Identities = 44/148 (29%), Positives = 70/148 (46%), Gaps = 3/148 (2%)
Frame = -1
Query: 235 RKIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVAD 294
+KI+ I W + GGLG+ ++ N ALL KW W + LW ++L+++Y
Sbjct: 418 KKIAWISWRQCCASGDVGGLGIKDIKILNNALLIKWKWLMFHQPHQLWNRILISKY-KGW 242
Query: 295 GGLEDGDLEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVR--RRVGDGAKIDFWRDRW 352
GL+ G + S WW ++ I + A + +VG G +I FW D W
Sbjct: 241 RGLDQGPQK---YYFSPWWADLRAINQ-----HQSMIAASNQFCWKVGRGDQILFWEDSW 86
Query: 353 CGD-VPLCDRFSRLYDLAVNKSITVRNM 379
D PL D+F LY L+ ++ + +M
Sbjct: 85 VDDGTPLKDQFPELYRLSSQRNFIMADM 2
>CO982349
Length = 795
Score = 61.2 bits (147), Expect = 9e-10
Identities = 23/55 (41%), Positives = 40/55 (71%)
Frame = +3
Query: 193 LLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSL 247
L+ ++LTALP+Y SFF+APS ++ + ++ KF WG G +R+I+ ++W+T+ L
Sbjct: 627 LINAILTALPIYFFSFFRAPSKVVQKLVNIQRKFLWGGGSXQRRIAWVKWETVCL 791
>BI972659
Length = 453
Score = 50.4 bits (119), Expect = 2e-06
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +1
Query: 193 LLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS 238
L+KSVL ALP+Y LSFFK P I+ + SL F WG + +IS
Sbjct: 310 LIKSVLNALPIYLLSFFKIPQRIVDKLVSLQRTFMWGGNQHHNRIS 447
>BI427218
Length = 421
Score = 49.3 bits (116), Expect = 4e-06
Identities = 36/140 (25%), Positives = 62/140 (43%), Gaps = 3/140 (2%)
Frame = +1
Query: 268 GKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLED--GGRSCSSWWREIVRIRDGICE 325
GKW W ++ LW ++L ++YG + LE+ G S+WW++I I +
Sbjct: 1 GKWKWSFFYNKGELWARILDSKYG------DWRHLEEPTRGNRASAWWKDINIIDPSGAD 162
Query: 326 GGEGWFGACVRRRVGDGAKIDFWRDRWCGD-VPLCDRFSRLYDLAVNKSITVRNMFLLGV 384
G F ++ +V G K FW D W D V ++ L+ ++ ++ M
Sbjct: 163 G--SLFDKGIKWKVACGEKTKFWEDGWKEDRVTFKLKYPTLFSISKQ*QHLIKMMGNFTA 336
Query: 385 DVGGEALRWRRRLWAWEEEL 404
+WRR+L+ E E+
Sbjct: 337 TGWEWNFKWRRQLFDDEMEM 396
>TC231922
Length = 803
Score = 43.5 bits (101), Expect = 2e-04
Identities = 29/124 (23%), Positives = 61/124 (48%), Gaps = 4/124 (3%)
Frame = -3
Query: 310 SSWWREIVRIRDGICEGGEGWFGACVRR---RVGDGAKIDFWRDRWCGD-VPLCDRFSRL 365
S WW+++ R+ + + F + + RVG G KI FW+D W + L ++++L
Sbjct: 777 SHWWKDLRRLYN------QPDFHSIHQNMVWRVGCGDKIKFWQDSWLSEGCNLQQKYNQL 616
Query: 366 YDLAVNKSITVRNMFLLGVDVGGEALRWRRRLWAWEEELVEECRVVLLTVSLKSDK*MAM 425
Y ++ ++T+ M + +WRRRL+ +E + + + +S+++ +M
Sbjct: 615 YTISRQ*NLTISKMGKFSQNARSWEFKWRRRLFDYEYAMAVDFMNEISGISIQNQAHDSM 436
Query: 426 VTKS 429
K+
Sbjct: 435 FWKA 424
>CO978908
Length = 782
Score = 41.2 bits (95), Expect = 0.001
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +1
Query: 270 WCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSSWWREI 316
W W L D++ LW +++ ++Y GG E+ R CS WWR++
Sbjct: 604 WIWALASDQQQLWARIINSKY----GGWEEFQSGRDKRGCSHWWRDL 732
>TC222557
Length = 1002
Score = 40.0 bits (92), Expect = 0.002
Identities = 23/71 (32%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
Frame = +3
Query: 329 GWFGACVRRRVGDGAKIDFWRDRWCGD-VPLCDRFSRLYDLAVNKSITVRNMFLLGVDVG 387
G F + + +VG G KI FW D W + PL ++ RLY+++ + + M +V
Sbjct: 45 GLFQSAIDWKVGCGDKIRFWEDCWLMEQEPLRAKYPRLYNISCQQQKLILVMGFHSANVW 224
Query: 388 GEALRWRRRLW 398
L WRR L+
Sbjct: 225 EWKLEWRRHLF 257
>BU090318 weakly similar to GP|9743345|gb| F21J9.30 {Arabidopsis thaliana},
partial (1%)
Length = 422
Score = 37.7 bits (86), Expect = 0.011
Identities = 30/117 (25%), Positives = 51/117 (42%)
Frame = +3
Query: 236 KIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADG 295
K+ W L K+ GGLG+ L N +LL K + L +V+ ++Y
Sbjct: 3 KVHWTSWKALCSPKKSGGLGLRLLSHMNKSLLMKST*NMCTKPNDL*VRVVRSKYK*GKD 182
Query: 296 GLEDGDLEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWRDRW 352
L D + G +++W+ IV + I + R++G+G + FW+D W
Sbjct: 183 ILP*IDPKKTG---TNFWQGIVHCWEKITHDN-------IARKIGNGRSVHFWKDVW 323
>AI496436
Length = 414
Score = 35.4 bits (80), Expect = 0.053
Identities = 18/37 (48%), Positives = 23/37 (61%), Gaps = 2/37 (5%)
Frame = +1
Query: 182 WLAKSVPFFR--ILLKSVLTALPVYALSFFKAPSGII 216
W K V F L+ SVLTALP+Y LSFF+ P ++
Sbjct: 232 WKQKYVSFGGRVTLINSVLTALPIYLLSFFRIPDKVV 342
>CF920672
Length = 792
Score = 32.3 bits (72), Expect = 0.45
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = -2
Query: 212 PSGIISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSLRKE 250
P+ + + + +F WG G D++KI+ ++WD + +E
Sbjct: 791 PNRVAEKLTQIQRRFXWGGGLDQKKIAWVKWDC*LMEQE 675
>BM309783
Length = 433
Score = 32.0 bits (71), Expect = 0.58
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +2
Query: 229 GEGEDKRKIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVA 288
G+ + RK + W + + GG+G+ L N A L K W + +LW VL
Sbjct: 8 GDNDGGRKHHVVGWKAMIMP*HSGGVGMCDLTIMNQACLMKIGWAFRMGDGSLWADVLRG 187
Query: 289 RYG 291
YG
Sbjct: 188 *YG 196
>BQ297454
Length = 135
Score = 31.6 bits (70), Expect = 0.76
Identities = 10/31 (32%), Positives = 18/31 (57%)
Frame = -1
Query: 198 LTALPVYALSFFKAPSGIISSIKSLLNKFFW 228
LT++ +Y SFF+ P ++ + + KF W
Sbjct: 108 LTSISIYFFSFFRVPKKVVDKLARIQRKFLW 16
>BF596367
Length = 419
Score = 31.2 bits (69), Expect = 0.99
Identities = 14/33 (42%), Positives = 23/33 (69%)
Frame = +1
Query: 258 RLREFNVALLGKWCWRLLLDREALWRKVLVARY 290
R++ F+ A+L K WRL+ +++AL VL A+Y
Sbjct: 61 RIKAFHKAMLAKQMWRLIDNKDALVT*VLTAKY 159
>BM893063
Length = 422
Score = 30.8 bits (68), Expect = 1.3
Identities = 13/27 (48%), Positives = 14/27 (51%)
Frame = -2
Query: 306 GRSCSSWWREIVRIRDGICEGGEGWFG 332
GR C WWR V C GG GW+G
Sbjct: 229 GRWCWCWWRHWVH-----CGGGSGWWG 164
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.353 0.160 0.608
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,249,861
Number of Sequences: 63676
Number of extensions: 371715
Number of successful extensions: 5441
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 4050
Number of HSP's successfully gapped in prelim test: 163
Number of HSP's that attempted gapping in prelim test: 1201
Number of HSP's gapped (non-prelim): 4441
length of query: 448
length of database: 12,639,632
effective HSP length: 100
effective length of query: 348
effective length of database: 6,272,032
effective search space: 2182667136
effective search space used: 2182667136
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC138580.5