BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138464.6 + phase: 0
(250 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC226798 weakly similar to UP|Q8W575 (Q8W575) AT5g14250/F18O22_4... 368 e-102
CA934990 138 2e-33
TC215393 homologue to UP|PSD3_DAUCA (Q06364) Probable 26S protea... 46 2e-05
TC215392 similar to UP|PSD3_TOBAC (P93768) Probable 26S proteaso... 41 6e-04
TC214660 similar to UP|Q6XJG1 (Q6XJG1) 26S proteasome subunit RP... 35 0.025
TC214667 similar to UP|Q6XJG1 (Q6XJG1) 26S proteasome subunit RP... 32 0.21
TC214672 similar to GB|AAP86663.1|32700026|AY230836 26S proteaso... 32 0.21
CD413771 29 1.8
AI496547 similar to PIR|T05735|T05 cytochrome P450 71A10 - soybe... 29 1.8
TC221167 weakly similar to UP|Q948Q7 (Q948Q7) Anthocyanin-relate... 27 6.7
AW133328 weakly similar to PIR|T10955|T10 early nodulin binding ... 27 8.8
>TC226798 weakly similar to UP|Q8W575 (Q8W575) AT5g14250/F18O22_40, partial
(86%)
Length = 1572
Score = 368 bits (944), Expect = e-102
Identities = 186/219 (84%), Positives = 200/219 (90%)
Frame = +1
Query: 1 MICIGQKRFQKALDLLHNVVTAPMTMMNAIVIEAYKKYILVSLIRHGQFSTSLPKYASPL 60
MICIGQK FQKALDLLHNVVTAPM+ +NAI +EAYKKYILVSLIRHGQFSTSLPKY+S
Sbjct: 637 MICIGQKHFQKALDLLHNVVTAPMSTINAIAVEAYKKYILVSLIRHGQFSTSLPKYSSST 816
Query: 61 AQRNLKLFCQNPCIELAQTYNNGKVAEVEAFVNANAGRFEADNNLGLAKQVVSSMYKRNI 120
AQRNLK FCQ P +ELA +Y G++ E+EA+ ANA +FE+DNNLGL QVVSSMYKRNI
Sbjct: 817 AQRNLKNFCQ-PYVELANSYGTGQIEELEAYARANAEKFESDNNLGLVNQVVSSMYKRNI 993
Query: 121 QRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQY 180
QRLTQTYLTLSL+DIANTV LNS KEAEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQY
Sbjct: 994 QRLTQTYLTLSLQDIANTVQLNSPKEAEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQY 1173
Query: 181 KTCEMIEHIDSSIQRIMALSKKLTATDEQISCDQLYLSK 219
KTCEMIEHIDSSIQRIMALS+KLTATDEQISCDQLYLSK
Sbjct: 1174KTCEMIEHIDSSIQRIMALSRKLTATDEQISCDQLYLSK 1290
>CA934990
Length = 243
Score = 138 bits (348), Expect = 2e-33
Identities = 70/78 (89%), Positives = 72/78 (91%)
Frame = +1
Query: 128 LTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQYKTCEMIE 187
LTLSL +ANT LNS KE EMHVLQMIQDGEIYATINQKDGMVRFLEDPEQYKTCEMIE
Sbjct: 10 LTLSLRHMANTGQLNSPKEGEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQYKTCEMIE 189
Query: 188 HIDSSIQRIMALSKKLTA 205
HIDSSIQRIMALS+KLTA
Sbjct: 190 HIDSSIQRIMALSRKLTA 243
>TC215393 homologue to UP|PSD3_DAUCA (Q06364) Probable 26S proteasome
non-ATPase regulatory subunit 3 (26S proteasome subunit
S3) (Nuclear antigen 21D7), partial (81%)
Length = 1500
Score = 45.8 bits (107), Expect = 2e-05
Identities = 32/116 (27%), Positives = 55/116 (46%), Gaps = 3/116 (2%)
Frame = +1
Query: 72 PCIELAQTYNNGKVAEVEAFVNANAGRFEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLS 131
P EL G + + A F AD L ++ ++ + ++ ++ +Y +S
Sbjct: 655 PYFELTNAVRIGDLELFRNIADKFATTFNADRTHNLIVRLRHNVIRTGLRNISISYSRIS 834
Query: 132 LEDIANTVHLNSAK---EAEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQYKTCE 184
L D+A + LNSA +AE V + I+DG I AT++ +G + E + Y T E
Sbjct: 835 LADVAKKLRLNSASPVADAESIVSKAIRDGAIDATLDHANGWMVSKETGDIYSTNE 1002
>TC215392 similar to UP|PSD3_TOBAC (P93768) Probable 26S proteasome
non-ATPase regulatory subunit 3 (26S proteasome subunit
S3) (Nuclear antigen 21D7), partial (45%)
Length = 985
Score = 40.8 bits (94), Expect = 6e-04
Identities = 29/110 (26%), Positives = 55/110 (49%), Gaps = 6/110 (5%)
Frame = +3
Query: 81 NNGKVAEVEAFVNAN---AGRFEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLSLEDIAN 137
N ++ ++E F N F D L ++ ++ + ++ ++ +Y +SL D+A
Sbjct: 150 NAVRIGDLELFRNVAEKFGTTFNRDRTHNLIVRLRHNVIRTGLRNISISYSRISLVDVAK 329
Query: 138 TVHLNSAK---EAEMHVLQMIQDGEIYATINQKDGMVRFLEDPEQYKTCE 184
+ LNSA +AE V + I+DG I A+++ +G + E + Y T E
Sbjct: 330 KLRLNSASPVADAESIVAKAIRDGAIDASLDHANGWMVSKETGDIYSTNE 479
>TC214660 similar to UP|Q6XJG1 (Q6XJG1) 26S proteasome subunit RPN6a
(Fragment), partial (42%)
Length = 778
Score = 35.4 bits (80), Expect = 0.025
Identities = 25/96 (26%), Positives = 44/96 (45%), Gaps = 4/96 (4%)
Frame = +1
Query: 114 SMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRF 173
S+ ++N+ RL + Y + + IA + L E + QMI D + T++Q G +
Sbjct: 226 SLMEQNLWRLIEPYARVEIAHIAELIKL-PVDHVEQKLSQMILDKKFVGTLDQGAGCLVI 402
Query: 174 LEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
+DP+ T E I +I + + S K+ A
Sbjct: 403 FDDPKTDAIYPATLETIFNIGKVVDSLYVRSAKIVA 510
>TC214667 similar to UP|Q6XJG1 (Q6XJG1) 26S proteasome subunit RPN6a
(Fragment), partial (46%)
Length = 757
Score = 32.3 bits (72), Expect = 0.21
Identities = 25/102 (24%), Positives = 48/102 (46%), Gaps = 8/102 (7%)
Frame = +2
Query: 112 VSSMY----KRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQK 167
+SS+Y ++N+ RL + + + + IA + L + E + QMI D + T++Q
Sbjct: 257 LSSLYDTLMEQNLCRLIEPFSRVEIAHIAELIELPT-DHVERKLSQMILDKKFAGTLDQG 433
Query: 168 DGMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
G + +DP+ T E I ++ + + S K+ A
Sbjct: 434 AGCLVIFDDPKTDAIYPATLETISNVGKVVDSLYVRSAKIMA 559
>TC214672 similar to GB|AAP86663.1|32700026|AY230836 26S proteasome subunit
RPN6a {Arabidopsis thaliana;} , partial (95%)
Length = 1703
Score = 32.3 bits (72), Expect = 0.21
Identities = 25/102 (24%), Positives = 48/102 (46%), Gaps = 8/102 (7%)
Frame = +2
Query: 112 VSSMY----KRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQK 167
+SS+Y ++N+ RL + + + + IA + L + E + QMI D + T++Q
Sbjct: 1166 LSSLYDTLMEQNLCRLIEPFSRVEIAHIAELIELPT-DHVERKLSQMILDKKFAGTLDQG 1342
Query: 168 DGMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
G + +DP+ T E I ++ + + S K+ A
Sbjct: 1343 AGCLVIFDDPKTDAIYPATLETISNVGKVVDSLYVRSAKIMA 1468
>CD413771
Length = 679
Score = 29.3 bits (64), Expect = 1.8
Identities = 19/70 (27%), Positives = 36/70 (51%), Gaps = 4/70 (5%)
Frame = +1
Query: 112 VSSMY----KRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQK 167
+SS+Y ++N+ RL + + + + IA + L + E + QMI D + T++Q
Sbjct: 472 LSSLYDTLMEQNLCRLIEPFSRVEIAHIAELIELPT-DHVERKLSQMILDKKFAGTLDQG 648
Query: 168 DGMVRFLEDP 177
G + +DP
Sbjct: 649 AGCLVIFDDP 678
>AI496547 similar to PIR|T05735|T05 cytochrome P450 71A10 - soybean, partial
(22%)
Length = 415
Score = 29.3 bits (64), Expect = 1.8
Identities = 18/43 (41%), Positives = 26/43 (59%), Gaps = 1/43 (2%)
Frame = +1
Query: 23 PMTMMNAIVIEAYKKYILVSL-IRHGQFSTSLPKYASPLAQRN 64
P+T + +++EA I +SL HG+FS SLP +AS A N
Sbjct: 289 PITHLETMLLEAA---ISISLRFTHGRFSLSLPPHASRTASTN 408
>TC221167 weakly similar to UP|Q948Q7 (Q948Q7) Anthocyanin-related membrane
protein 3, partial (33%)
Length = 757
Score = 27.3 bits (59), Expect = 6.7
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = -3
Query: 174 LEDPEQYKTCEMIEHI 189
LEDP YKTCE +H+
Sbjct: 656 LEDPLNYKTCEQEQHL 609
>AW133328 weakly similar to PIR|T10955|T10 early nodulin binding protein 1 -
spring vetch, partial (2%)
Length = 421
Score = 26.9 bits (58), Expect = 8.8
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = -3
Query: 51 TSLPKYASPLAQRNLKLFCQNPCIELAQTY 80
T P Y +PLA L CQN C +L Q Y
Sbjct: 131 TPFPFYENPLACHAFSLPCQN-CFQLLQPY 45
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.133 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,061,893
Number of Sequences: 63676
Number of extensions: 99028
Number of successful extensions: 470
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 469
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 469
length of query: 250
length of database: 12,639,632
effective HSP length: 95
effective length of query: 155
effective length of database: 6,590,412
effective search space: 1021513860
effective search space used: 1021513860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC138464.6