BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138464.11 - phase: 0
(292 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218052 similar to UP|2A5D_YEAST (P38903) Serine/threonine prot... 134 4e-32
TC230472 107 8e-24
BG507351 105 2e-23
BU965106 homologue to PIR|H86399|H86 protein F17L21.26 [imported... 35 0.053
TC231669 homologue to UP|Q9FZI9 (Q9FZI9) F17L21.26, partial (5%) 33 0.15
TC218749 weakly similar to GB|AAP37705.1|30725366|BT008346 At1g6... 30 1.3
TC222094 30 1.7
AW132336 28 6.4
AW705531 28 6.4
TC218704 similar to UP|KEUL_ARATH (Q9C5X3) SNARE-interacting pro... 28 6.4
TC227159 28 6.4
TC214664 weakly similar to GB|AAL31248.1|16974489|AY061921 At2g2... 28 6.4
TC216566 similar to UP|Q8S9A1 (Q8S9A1) Glucosyltransferase-8 (Fr... 27 8.4
TC211089 weakly similar to UP|O23517 (O23517) LIMONENE cyclase l... 27 8.4
>TC218052 similar to UP|2A5D_YEAST (P38903) Serine/threonine protein
phosphatase 2A, 56 kDa regulatory subunit, delta isoform
(PP2A, B subunit, B' delta isoform) (RTS1 protein) (SCS1
protein), partial (3%)
Length = 1248
Score = 134 bits (338), Expect = 4e-32
Identities = 104/286 (36%), Positives = 147/286 (51%), Gaps = 21/286 (7%)
Frame = +1
Query: 28 IMLRFRPIAPKPV---TGEQTPANQSTTASLPGKRTKRKYVRIRRNNGYIKKNSCKKSSP 84
IMLRFRPIAPKP T +++S+ A L TKRKYVR +N Y K ++
Sbjct: 112 IMLRFRPIAPKPAAAATASDGSSSESSDAFLKNGTTKRKYVR---DNNYTSKRRIRR--- 273
Query: 85 EEEIVVQHSNSNDEMKRNLAVVTLQLMPEKAAATSN--VDLNLNLNLTVENIQILDNLNR 142
N++ K+ VVTL L+PE + DL + V N N N
Sbjct: 274 -------RKNTSSPEKKQKPVVTLPLLPETPDRKDSRAKDLTPSPAKPVRN-----NNNN 417
Query: 143 SQNNPDLETMNAGKFLDLLPAVEK---AVVESW---ITVESVTGMCMVVE--------KV 188
+ + +L +N K + + + E ++ W +TVE VT C+ E +
Sbjct: 418 NNKSKNLNIIN--KNVPVWVSFENRSLTMMGGWCSCVTVEGVTDTCLESEWLGSTDEERR 591
Query: 189 KNIETDTCPCFITDGNGKVLRVNDAYKKMVVNE-ESEEVEVVIWLKVKQSVAWCYSYPAF 247
N+ DTCP FI+DG G+V N+AY KM++ + E +E + + L K + + +F
Sbjct: 592 LNLSNDTCPGFISDGYGRVTWTNEAYGKMMMMKGEGDEGQGPVLLVNKVNTV--VPHASF 765
Query: 248 TCGVRL-QYTWRNEKCLKMVPCDVWRLECGGFAWRLDVKAALSLGL 292
TC VR+ QY+ E+ VPCDVWR++CGGFAWRLDVK ALSL L
Sbjct: 766 TCLVRVVQYSCGKERNSLTVPCDVWRMDCGGFAWRLDVKTALSLRL 903
>TC230472
Length = 1029
Score = 107 bits (266), Expect = 8e-24
Identities = 86/272 (31%), Positives = 132/272 (47%), Gaps = 7/272 (2%)
Frame = +3
Query: 28 IMLRFRPIAPKPVTGEQTPANQSTTASLPGKRTKRKYVRIRR-NNGYIKKNSCKKSSPEE 86
IMLRFRPIAPKP+ + ++++ G ++ +R + G ++ + P
Sbjct: 60 IMLRFRPIAPKPLPAAAAALSDASSSESTGSAKRKDNTASKRCSRGIRRRRNAPPPPPSP 239
Query: 87 EIVVQHSNSNDEMKRNLAVVTLQLMPEKAAATSNVDLNLNLNLTVEN-----IQILDNLN 141
+ + + K+ +T +L NV + L+ EN + LD
Sbjct: 240 AVTLPLLPGSPGPKK----ITSELK------NKNVPVWLSFENNFENRGGAASEKLDPCW 389
Query: 142 RSQNNPDLETMNAGKFLDLLPAVEKAVVESWITVESVTGMCMVVEKVK-NIETDTCPCFI 200
SQ T AG + + + V+++W + G+ E+ K ++ DTCP FI
Sbjct: 390 YSQ-----ATAAAGSVVTV-----ECVMDTWQQQDEGLGLGSGDEERKVKLKEDTCPGFI 539
Query: 201 TDGNGKVLRVNDAYKKMVVNEESEEVEVVIWLKVKQSVAWCYSYPAFTCGVRLQYTWRNE 260
+DG G+V N+AY++ V +WL +K +V Y Y FTC VR++Y E
Sbjct: 540 SDGYGRVTWTNEAYRETVGAGG-------VWLAMKVAVP--YPYRGFTCRVRVRYACGIE 692
Query: 261 KCLKMVPCDVWRLECGGFAWRLDVKAALSLGL 292
+ VPCDVWR++ GGFAWRLDVKAALSL L
Sbjct: 693 RT---VPCDVWRMDSGGFAWRLDVKAALSLSL 779
>BG507351
Length = 438
Score = 105 bits (262), Expect = 2e-23
Identities = 68/146 (46%), Positives = 89/146 (60%), Gaps = 20/146 (13%)
Frame = -1
Query: 94 NSNDEMKRNLAVVTLQLMPEKAAATSNVDL-------NLNLNLTVENIQILDNLNRSQNN 146
N NDE ++AVVTLQLMPEK A +V L N++L+LTVE IQI++N +
Sbjct: 432 NCNDESS-DVAVVTLQLMPEKDAPEGDVTLAGDSWCKNVDLDLTVEKIQIVENRSVPPPR 256
Query: 147 PDLETMNAGKFLDLLPAVEKAVVESWITVESVTGMCMVV-------------EKVKNIET 193
+E K DL+PA + A ESW+TVESVTG CM E+VK++ET
Sbjct: 255 LVVEEGEGAKGSDLVPAAKAA--ESWVTVESVTGTCMGEGEGGRGLLSCTDEERVKSLET 82
Query: 194 DTCPCFITDGNGKVLRVNDAYKKMVV 219
DTCP F+ DG+ +V VNDAYK+MV+
Sbjct: 81 DTCPGFVCDGSLRVRWVNDAYKRMVL 4
>BU965106 homologue to PIR|H86399|H86 protein F17L21.26 [imported] -
Arabidopsis thaliana, partial (3%)
Length = 431
Score = 34.7 bits (78), Expect = 0.053
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +2
Query: 28 IMLRFRPIAPKPVTGEQTPANQSTTASLPGKRTKRKYVR 66
IM R+RPIAPKP T + ++ + +SL K + Y+R
Sbjct: 113 IMSRYRPIAPKPETSPNSSMSEGSCSSLSQKINQSPYLR 229
>TC231669 homologue to UP|Q9FZI9 (Q9FZI9) F17L21.26, partial (5%)
Length = 596
Score = 33.1 bits (74), Expect = 0.15
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +1
Query: 28 IMLRFRPIAPKPVTGEQTPANQSTTASLPGKRTKRKYVR 66
IM R+RPIAPKP T + + +++SL K + Y+R
Sbjct: 121 IMSRYRPIAPKPETSPNSMSEGPSSSSLSQKIKQSPYLR 237
>TC218749 weakly similar to GB|AAP37705.1|30725366|BT008346 At1g61110
{Arabidopsis thaliana;} , partial (15%)
Length = 824
Score = 30.0 bits (66), Expect = 1.3
Identities = 22/70 (31%), Positives = 35/70 (49%), Gaps = 11/70 (15%)
Frame = +3
Query: 38 KPVTGEQTPAN--------QSTTASLPGKRTKRKYVRIRRNNGYIKK---NSCKKSSPEE 86
K +Q PA+ Q+ LPG+ + +Y R+NG IK+ + C KS +E
Sbjct: 192 KKAFAQQLPASVISEFDVFQTEPWKLPGELRENRYFFSNRSNGNIKRPAGSGCWKSVGKE 371
Query: 87 EIVVQHSNSN 96
+ ++ HS SN
Sbjct: 372 KQII-HSESN 398
>TC222094
Length = 984
Score = 29.6 bits (65), Expect = 1.7
Identities = 29/104 (27%), Positives = 44/104 (41%), Gaps = 18/104 (17%)
Frame = -3
Query: 40 VTGEQTPANQSTTASLPGK---RTKRKYVRIRRN---NGYIKKNSCKKSSPEE-----EI 88
+ G Q + Q AS GK +T + + + N N Y+ + S EE E+
Sbjct: 349 IDGMQPHSKQLLDASAGGKIKLKTPEEAIELIENMAANDYVILRDQEPSPQEESTRLEEL 170
Query: 89 VVQ-------HSNSNDEMKRNLAVVTLQLMPEKAAATSNVDLNL 125
+VQ H S D RNL V +L+PE+ T V+ +
Sbjct: 169 LVQFMQETRSHQKSTDAAIRNLEVQLAKLVPERPTETFAVNTKM 38
>AW132336
Length = 411
Score = 27.7 bits (60), Expect = 6.4
Identities = 15/57 (26%), Positives = 23/57 (40%)
Frame = -2
Query: 222 ESEEVEVVIWLKVKQSVAWCYSYPAFTCGVRLQYTWRNEKCLKMVPCDVWRLECGGF 278
E EV + +V WC + Y + C +++PC + R CGGF
Sbjct: 371 EPRHPEVPVNAAAPTAV*WCRLSSVRKARDLVHYGLQGATCARLLPCVLIRDGCGGF 201
>AW705531
Length = 331
Score = 27.7 bits (60), Expect = 6.4
Identities = 18/53 (33%), Positives = 24/53 (44%)
Frame = +1
Query: 240 WCYSYPAFTCGVRLQYTWRNEKCLKMVPCDVWRLECGGFAWRLDVKAALSLGL 292
W S AF CG + + + +K KM+ VW L FAW D LG+
Sbjct: 94 WIVSNLAFDCG*TIDIS*KCKKLFKML---VWSLIFWVFAWDTDFCRNTVLGI 243
>TC218704 similar to UP|KEUL_ARATH (Q9C5X3) SNARE-interacting protein KEULE,
partial (52%)
Length = 1363
Score = 27.7 bits (60), Expect = 6.4
Identities = 15/71 (21%), Positives = 31/71 (43%)
Frame = +2
Query: 87 EIVVQHSNSNDEMKRNLAVVTLQLMPEKAAATSNVDLNLNLNLTVENIQILDNLNRSQNN 146
+ + + +++ E K L ++ + PEK ++L LT E+ ++NL
Sbjct: 389 KFLTTYEDTSRENKLRLLMILASIYPEKFEGEKGLNLMKVAKLTDEDTIAINNLRMLGGE 568
Query: 147 PDLETMNAGKF 157
PD + + F
Sbjct: 569 PDTKKTSTSSF 601
>TC227159
Length = 970
Score = 27.7 bits (60), Expect = 6.4
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = -2
Query: 155 GKFLDLLPAVEKAVVESWITVESVTGMCMVVEKVKNIETDTCPCFITDGN 204
GKF LLP + + + SV +VEK ++ T+ CP F +DGN
Sbjct: 441 GKFY-LLPRCSGHCICLVVCLLSVL*FVFIVEKANSVNTEHCP-FPSDGN 298
>TC214664 weakly similar to GB|AAL31248.1|16974489|AY061921
At2g27080/T20P8.13 {Arabidopsis thaliana;} , partial
(56%)
Length = 1097
Score = 27.7 bits (60), Expect = 6.4
Identities = 17/40 (42%), Positives = 22/40 (54%), Gaps = 6/40 (15%)
Frame = -3
Query: 31 RFR-PIAPKP-----VTGEQTPANQSTTASLPGKRTKRKY 64
RFR P+ P +TG +TPA + TAS P K +R Y
Sbjct: 687 RFRHPLTPSQTPSRCLTGGKTPAERRYTASRPYKTPRRSY 568
>TC216566 similar to UP|Q8S9A1 (Q8S9A1) Glucosyltransferase-8 (Fragment),
partial (32%)
Length = 925
Score = 27.3 bits (59), Expect = 8.4
Identities = 20/63 (31%), Positives = 30/63 (46%), Gaps = 7/63 (11%)
Frame = +1
Query: 28 IMLRFRPIAPKPVTGEQTPANQSTTASLPGKRTKRKYVRIRRNNGY-------IKKNSCK 80
+ L+FR P P + QTP +S AS +R R+ R R + ++ +SCK
Sbjct: 361 VPLQFRR-PPPPPSSSQTPPIRSGGASSQSRRKSRRRRRTTRGGCFSGCGPMRMRSSSCK 537
Query: 81 KSS 83
SS
Sbjct: 538 GSS 546
>TC211089 weakly similar to UP|O23517 (O23517) LIMONENE cyclase like protein,
partial (9%)
Length = 626
Score = 27.3 bits (59), Expect = 8.4
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = +3
Query: 223 SEEVEVVIWLKVKQSVAWCYSYPAFTCGVRLQYTWRNEKCLKM 265
S + IWL K S+ W + G+R TW N KM
Sbjct: 63 STSTDTPIWLLKKWSMPWSFHPIGEYSGLRSNGTWSNTNSRKM 191
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.132 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,373,488
Number of Sequences: 63676
Number of extensions: 202680
Number of successful extensions: 1137
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1125
length of query: 292
length of database: 12,639,632
effective HSP length: 96
effective length of query: 196
effective length of database: 6,526,736
effective search space: 1279240256
effective search space used: 1279240256
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC138464.11