BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138199.3 - phase: 0
(306 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC233634 similar to UP|Q8LKH0 (Q8LKH0) PIN1-like auxin transport... 389 e-109
TC227966 similar to UP|Q8H1L0 (Q8H1L0) PIN-like protein, partial... 373 e-104
BG839278 homologue to GP|25986771|gb auxin efflux carrier protei... 237 6e-63
TC211544 weakly similar to UP|Q84YG8 (Q84YG8) Auxin efflux carri... 221 4e-58
BE191508 similar to GP|10441744|gb| PIN1-like auxin transport pr... 92 2e-19
TC222695 weakly similar to UP|Q6ZIB5 (Q6ZIB5) PIN1-like auxin tr... 61 2e-12
TC227967 homologue to UP|Q84WX3 (Q84WX3) Auxin efflux carrier pr... 52 3e-07
BI470567 29 2.3
TC207176 29 2.3
AW759451 similar to GP|169989|gb|A NADPH-specific isocitrate deh... 29 3.0
TC205279 homologue to PRF|2206327A.0|1587206|2206327A T complex ... 28 4.0
AW309504 weakly similar to GP|169989|gb|AA NADPH-specific isocit... 28 4.0
BG352441 similar to GP|15215854|gb| At2g43170/F14B2.11 {Arabidop... 28 6.8
TC204411 similar to UP|Q9SJM6 (Q9SJM6) Expressed protein (Zinc f... 28 6.8
TC226345 27 8.9
CA784302 similar to GP|4102839|gb| LeOPT1 {Lycopersicon esculent... 27 8.9
TC228636 similar to UP|Q6NLX7 (Q6NLX7) At4g36660, partial (51%) 27 8.9
TC226347 27 8.9
>TC233634 similar to UP|Q8LKH0 (Q8LKH0) PIN1-like auxin transport protein,
partial (41%)
Length = 817
Score = 389 bits (999), Expect = e-109
Identities = 190/243 (78%), Positives = 219/243 (89%), Gaps = 2/243 (0%)
Frame = +2
Query: 1 MITLTDFYHVMTAMVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIA 60
MITL D YHV+TA+VPLYVAMILAYGSVKWWKIF+PDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 89 MITLWDLYHVLTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIS 268
Query: 61 SNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITLFSLSTLPNTLVMGIPL 120
+NNPY MN +F+AAD+LQK I+L +L +WS S RG LEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 269 TNNPYAMNYKFIAADSLQKAIVLAVLLVWSRTSSRGSLEWSITLFSLSTLPNTLVMGIPL 448
Query: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARLLIFEQFPDTAGSIVSIHVDSD 180
LKGMYG+ SG+LMVQIVVLQCIIWYTLMLF+FE+RGARLLI EQFPDTA SI+S VDSD
Sbjct: 449 LKGMYGDASGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIGEQFPDTAASIISFKVDSD 628
Query: 181 VMSLDGRTPLETDAEIKEDGKLHITVRKSNASRSDIYSRRSQGLS--SNTPRPSNLTNAE 238
++SLDG+ PL+T+AE+ +DGKLH+TVRKS +SRS+I+SRRS G + S TPRPSNLTNAE
Sbjct: 629 IISLDGKEPLQTEAEVGDDGKLHVTVRKSASSRSEIFSRRSHGPNSVSLTPRPSNLTNAE 808
Query: 239 IYS 241
IYS
Sbjct: 809 IYS 817
>TC227966 similar to UP|Q8H1L0 (Q8H1L0) PIN-like protein, partial (52%)
Length = 992
Score = 373 bits (958), Expect = e-104
Identities = 186/203 (91%), Positives = 196/203 (95%)
Frame = +1
Query: 45 FVALFAVPLLSFHFIASNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITL 104
FVALFAVPLLSFHFIASNNPY+MNLRFLAADTLQK+IIL LLA+WSN +KRGCLEW ITL
Sbjct: 4 FVALFAVPLLSFHFIASNNPYEMNLRFLAADTLQKIIILVLLAVWSNITKRGCLEWAITL 183
Query: 105 FSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARLLIFEQ 164
FSLSTLPNTLVMGIPLLKGMYG+FSGSLMVQIVVLQCIIWYTLMLF+FEFRGAR+LI EQ
Sbjct: 184 FSLSTLPNTLVMGIPLLKGMYGDFSGSLMVQIVVLQCIIWYTLMLFLFEFRGARMLISEQ 363
Query: 165 FPDTAGSIVSIHVDSDVMSLDGRTPLETDAEIKEDGKLHITVRKSNASRSDIYSRRSQGL 224
FPDTA SIVSIHVDSDVMSLDGR PLET+AEIKEDGKLH+TVRKSNASRSDI+SRRSQGL
Sbjct: 364 FPDTAASIVSIHVDSDVMSLDGRQPLETEAEIKEDGKLHVTVRKSNASRSDIFSRRSQGL 543
Query: 225 SSNTPRPSNLTNAEIYSLQSSRN 247
SS TPRPSNLTNAEIYSLQSSRN
Sbjct: 544 SSTTPRPSNLTNAEIYSLQSSRN 612
>BG839278 homologue to GP|25986771|gb auxin efflux carrier protein {Medicago
truncatula}, partial (19%)
Length = 744
Score = 237 bits (604), Expect = 6e-63
Identities = 114/143 (79%), Positives = 127/143 (88%)
Frame = +2
Query: 1 MITLTDFYHVMTAMVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIA 60
MIT DFY VM+A++PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 314 MITWGDFYTVMSAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 493
Query: 61 SNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITLFSLSTLPNTLVMGIPL 120
NNPY MN RF+AADTLQK+I+L LAIW+NFS G LEW IT+FSLSTLPNTLVMGIPL
Sbjct: 494 LNNPYAMNFRFIAADTLQKIIMLFALAIWTNFSANGSLEWMITIFSLSTLPNTLVMGIPL 673
Query: 121 LKGMYGEFSGSLMVQIVVLQCII 143
L MYGE+SG LMVQ+VV QCII
Sbjct: 674 LIAMYGEYSGLLMVQVVVXQCII 742
>TC211544 weakly similar to UP|Q84YG8 (Q84YG8) Auxin efflux carrier protein,
partial (26%)
Length = 573
Score = 221 bits (562), Expect = 4e-58
Identities = 105/165 (63%), Positives = 133/165 (79%)
Frame = +3
Query: 1 MITLTDFYHVMTAMVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIA 60
MI Y+++ A+VPLYV MILAY SV WWKIF+P+QCSGINR VAL++VP L+F+F+A
Sbjct: 78 MIDGVAIYNIVAALVPLYVPMILAYASVLWWKIFTPEQCSGINRLVALYSVPFLNFNFLA 257
Query: 61 SNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITLFSLSTLPNTLVMGIPL 120
NNPY MNLRF+AADTLQK+IIL L +WS F+K G L+ TITLFS ST PNTL++G+P
Sbjct: 258 FNNPYAMNLRFIAADTLQKVIILGALFLWSAFTKCGNLD*TITLFSFSTFPNTLIVGVPF 437
Query: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARLLIFEQF 165
L MYG+ SGSLM QI V+Q ++W+TLMLF++E+RGA+ LI QF
Sbjct: 438 LTAMYGDSSGSLMSQIFVMQGVVWFTLMLFLYEYRGAKSLISNQF 572
>BE191508 similar to GP|10441744|gb| PIN1-like auxin transport protein
{Populus tremula x Populus tremuloides}, partial (9%)
Length = 478
Score = 92.4 bits (228), Expect = 2e-19
Identities = 41/56 (73%), Positives = 49/56 (87%)
Frame = -3
Query: 1 MITLTDFYHVMTAMVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSF 56
MI+L D YHV+TA VPLYV M LAY SVKWWK+F+P+QCSGIN+FVA F+VP+LSF
Sbjct: 173 MISLADVYHVVTATVPLYVTMFLAYISVKWWKLFTPEQCSGINKFVANFSVPILSF 6
>TC222695 weakly similar to UP|Q6ZIB5 (Q6ZIB5) PIN1-like auxin transport
protein-like, partial (41%)
Length = 1059
Score = 60.8 bits (146), Expect(2) = 2e-12
Identities = 27/67 (40%), Positives = 43/67 (63%)
Frame = +2
Query: 59 IASNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITLFSLSTLPNTLVMGI 118
+ N+ MN F+AADT+ K II+ +LA+W+ + + W+I FSL L N +V+G+
Sbjct: 2 LVPNSARGMNFSFIAADTISKFIIMVVLALWAKCTPKCTFCWSIISFSLCNLTNAVVVGV 181
Query: 119 PLLKGMY 125
P++K MY
Sbjct: 182 PMVKPMY 202
Score = 28.9 bits (63), Expect(2) = 2e-12
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +1
Query: 132 LMVQIVVLQCIIWYTLMLFMFEF 154
L+VQ VLQ IW L+LF+ EF
Sbjct: 220 LVVQSSVLQATIWLPLLLFVMEF 288
>TC227967 homologue to UP|Q84WX3 (Q84WX3) Auxin efflux carrier protein PIN1,
partial (56%)
Length = 1532
Score = 52.4 bits (124), Expect = 3e-07
Identities = 25/26 (96%), Positives = 25/26 (96%)
Frame = +2
Query: 222 QGLSSNTPRPSNLTNAEIYSLQSSRN 247
QGLSS TPRPSNLTNAEIYSLQSSRN
Sbjct: 2 QGLSSTTPRPSNLTNAEIYSLQSSRN 79
>BI470567
Length = 421
Score = 29.3 bits (64), Expect = 2.3
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = +2
Query: 59 IASNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITLFSLSTLPNTLVM 116
+ S P + +L TL++ I++ A S++ + CL WT T S P L M
Sbjct: 104 VLSKTPTNYEIPYLPLHTLERTILIERGAFTSSYLEVFCLSWTHTKPSTKLSPKLLNM 277
>TC207176
Length = 1616
Score = 29.3 bits (64), Expect = 2.3
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = +2
Query: 2 ITLTDFYHVMTAMVPLYVAMILAYGSVKW--WKIFSPDQCSGINRFVALFAVPLLSF 56
IT T YH +V ++++G V+ +++ S D C G N+F LF P SF
Sbjct: 1355 ITTTICYHFS*FVVIKIRLQMISFGFVEESRYEVDSTDLCLGTNQFCFLFTYP*FSF 1525
>AW759451 similar to GP|169989|gb|A NADPH-specific isocitrate dehydrogenase
{Glycine max}, partial (37%)
Length = 544
Score = 28.9 bits (63), Expect = 3.0
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 156 GARLLIFEQFPDTAGSIVSIHVDSDVMSLD 185
G L+F+ PDT +S+H+D V LD
Sbjct: 106 GKNELVFD*LPDTHSHFISVHLDDGVDDLD 17
>TC205279 homologue to PRF|2206327A.0|1587206|2206327A T complex protein.
{Cucumis sativus;} , partial (52%)
Length = 838
Score = 28.5 bits (62), Expect = 4.0
Identities = 16/65 (24%), Positives = 28/65 (42%)
Frame = -3
Query: 36 PDQCSGINRFVALFAVPLLSFHFIASNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKR 95
PD+ + + A+ LL+ HF+ S N Y + +Q + C ++SN +
Sbjct: 815 PDKIPSHAQGIMKAALCLLNDHFVTSTNEYSYSP*VCTMFNVQHSVFCCTKGLFSNHTSL 636
Query: 96 GCLEW 100
L W
Sbjct: 635 SQLFW 621
>AW309504 weakly similar to GP|169989|gb|AA NADPH-specific isocitrate
dehydrogenase {Glycine max}, partial (12%)
Length = 160
Score = 28.5 bits (62), Expect = 4.0
Identities = 12/41 (29%), Positives = 23/41 (55%)
Frame = -1
Query: 145 YTLMLFMFEFRGARLLIFEQFPDTAGSIVSIHVDSDVMSLD 185
Y ++L + +G L+++ PD +S+HV+ +V LD
Sbjct: 139 YVIILHVQLQQGHNELVYD*IPDNHSHFISVHVEDEVDDLD 17
>BG352441 similar to GP|15215854|gb| At2g43170/F14B2.11 {Arabidopsis
thaliana}, partial (2%)
Length = 411
Score = 27.7 bits (60), Expect = 6.8
Identities = 11/23 (47%), Positives = 15/23 (64%)
Frame = -2
Query: 82 ILCLLAIWSNFSKRGCLEWTITL 104
++CL WS+FS LEW +TL
Sbjct: 80 LICLGLRWSSFSGSKSLEWRVTL 12
>TC204411 similar to UP|Q9SJM6 (Q9SJM6) Expressed protein (Zinc finger-like
protein), partial (68%)
Length = 1346
Score = 27.7 bits (60), Expect = 6.8
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = -2
Query: 31 WKIFSPDQCSGINRFVALFAVPLLSFHFIASNNPYKMNL 69
+ +F+P QC NRF + + + S+ +N P ++ L
Sbjct: 1009 YDLFNPSQCRHNNRFFFFYTLEIGSYQRSTTNGPDQLTL 893
>TC226345
Length = 1680
Score = 27.3 bits (59), Expect = 8.9
Identities = 8/12 (66%), Positives = 10/12 (82%)
Frame = +3
Query: 29 KWWKIFSPDQCS 40
KWW++ SP QCS
Sbjct: 180 KWWRVCSPSQCS 215
>CA784302 similar to GP|4102839|gb| LeOPT1 {Lycopersicon esculentum}, partial
(22%)
Length = 436
Score = 27.3 bits (59), Expect = 8.9
Identities = 13/52 (25%), Positives = 25/52 (48%)
Frame = +2
Query: 54 LSFHFIASNNPYKMNLRFLAADTLQKLIILCLLAIWSNFSKRGCLEWTITLF 105
L++HF++ + + + R+L L LC L W+ + C+ +I F
Sbjct: 161 LAWHFLSHTSHWSRSSRWLLGSILDNCCFLCGLFHWNVYLDTFCIFTSIEAF 316
>TC228636 similar to UP|Q6NLX7 (Q6NLX7) At4g36660, partial (51%)
Length = 446
Score = 27.3 bits (59), Expect = 8.9
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = +1
Query: 80 LIILCLLAIWSNFSKRGCLEWTITLFSLST 109
L + LLA WS F+ L W+ TL SLS+
Sbjct: 226 LAAILLLAFWSMFTGTVSLRWSGTLNSLSS 315
>TC226347
Length = 401
Score = 27.3 bits (59), Expect = 8.9
Identities = 8/12 (66%), Positives = 10/12 (82%)
Frame = +2
Query: 29 KWWKIFSPDQCS 40
KWW++ SP QCS
Sbjct: 77 KWWRVSSPSQCS 112
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.137 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,330,219
Number of Sequences: 63676
Number of extensions: 176700
Number of successful extensions: 1585
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1573
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1584
length of query: 306
length of database: 12,639,632
effective HSP length: 97
effective length of query: 209
effective length of database: 6,463,060
effective search space: 1350779540
effective search space used: 1350779540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC138199.3