BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138130.21 - phase: 0 /pseudo
(247 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209395 331 2e-91
TC210074 104 3e-23
TC217566 similar to UP|Q8VYJ4 (Q8VYJ4) AT3g25150/MJL12_9, partia... 35 0.041
TC211828 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-pho... 34 0.070
CK606481 33 0.092
TC205645 UP|Q8RUD9 (Q8RUD9) Seed coat BURP domain protein 1, com... 32 0.27
TC214758 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-pho... 31 0.59
TC218363 similar to UP|O73592 (O73592) CMIX, partial (11%) 30 0.78
TC211475 similar to UP|P79122 (P79122) Pinin, partial (5%) 30 1.0
TC222075 similar to UP|Q84XC0 (Q84XC0) Calcineurin B-like-intera... 30 1.3
AW200869 similar to GP|16604527|gb| AT5g51550/K17N15_10 {Arabido... 29 1.7
TC228613 weakly similar to UP|PE31_ARATH (Q9LHA7) Peroxidase 31 ... 29 2.3
TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Pro... 28 3.0
TC208144 similar to UP|Q8VYU3 (Q8VYU3) GTP cyclohydrolase I , p... 28 3.0
TC228906 similar to UP|Q6ID88 (Q6ID88) At3g26510, partial (48%) 28 3.0
TC216068 similar to UP|Q9ZU51 (Q9ZU51) RING-H2 finger protein RH... 28 3.9
BG507959 homologue to GP|3287690|gb|A T22J18.15 {Arabidopsis tha... 28 3.9
TC206965 28 3.9
TC216067 weakly similar to UP|RH2A_ARATH (Q9ZT50) RHA2a protein ... 28 3.9
BE661546 27 8.6
>TC209395
Length = 710
Score = 331 bits (848), Expect = 2e-91
Identities = 165/192 (85%), Positives = 174/192 (89%), Gaps = 1/192 (0%)
Frame = +3
Query: 1 MSTTAQRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPPPPFF 60
MSTT QRTPRS R SLFFQDLASPV+ARRGKFSSPGQ+ A SSLWRENFG+SDLPPPP F
Sbjct: 135 MSTTVQRTPRSSRHSLFFQDLASPVSARRGKFSSPGQAAAVSSLWRENFGSSDLPPPPVF 314
Query: 61 TLEDRSDFSPESGILDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEASTSYALRGVQQN 120
TLEDRSDFSPESGILDYQ+SPE KSD +TPIQT N+EFSTP K KSEASTSYALRGVQQN
Sbjct: 315 TLEDRSDFSPESGILDYQISPEAKSDTRTPIQTSNKEFSTPLKSKSEASTSYALRGVQQN 494
Query: 121 QQGSLSLNWWS-SPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNSLPA 179
QQGS L+WWS + AKS GEQ+EKGR SPVEGVVQPGALITLPPP EVARPEVQRNSLPA
Sbjct: 495 QQGSPGLSWWSPTTAKSVGEQDEKGRSSPVEGVVQPGALITLPPPLEVARPEVQRNSLPA 674
Query: 180 GNLNEEEWVTVY 191
GNLNEEEWVTVY
Sbjct: 675 GNLNEEEWVTVY 710
>TC210074
Length = 547
Score = 104 bits (260), Expect = 3e-23
Identities = 47/54 (87%), Positives = 50/54 (92%)
Frame = +1
Query: 194 SPNDTNLVLREFEKCGEILKHVPGPRDANWMHILYQNRSAAQNALNKNGIQING 247
SP DTN+VLREFEKCGEILKHVPGPRDANW+HILYQNRS A ALNKNG+QING
Sbjct: 1 SPGDTNMVLREFEKCGEILKHVPGPRDANWVHILYQNRSDAHKALNKNGMQING 162
>TC217566 similar to UP|Q8VYJ4 (Q8VYJ4) AT3g25150/MJL12_9, partial (20%)
Length = 1031
Score = 34.7 bits (78), Expect = 0.041
Identities = 29/98 (29%), Positives = 44/98 (44%), Gaps = 9/98 (9%)
Frame = +3
Query: 159 ITLPPPPEVARPE---VQRNSLPAGNLNEEEWVTVY--GFSPNDTNLVLR-EFEKCGEIL 212
I PPP ++ + N + E E ++Y G P T VL EF+K G I
Sbjct: 108 IAAPPPSSISETNGSIINTNEVGNNQETEAEGYSIYVKGLPPTATPAVLENEFKKFGPIK 287
Query: 213 K---HVPGPRDANWMHILYQNRSAAQNALNKNGIQING 247
V + ++ + ++ SAAQ+AL + I ING
Sbjct: 288 SGGIQVRSQKGFSYGFVEFEVASAAQSALEASPISING 401
>TC211828 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate
isomerase (Phosphoriboisomerase) , partial (47%)
Length = 729
Score = 33.9 bits (76), Expect = 0.070
Identities = 34/121 (28%), Positives = 50/121 (41%), Gaps = 12/121 (9%)
Frame = +2
Query: 3 TTAQRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPPPPFFTL 62
TT++++P + R S S +A S+P + ASSS + +S+ PPPP T
Sbjct: 368 TTSRKSPPT-RPSSTSSPAWSSASAPAPLPSTPSTASASSSAKENSKTSSESPPPPKPTT 544
Query: 63 EDRSDFSPE--SGILDYQMSPETKSDHKTPIQTPNREFS----------TPAKGKSEAST 110
SP S +SP T P T +R + PA+ S +ST
Sbjct: 545 RPSPSASPSPISTPTPPSISPSTAPTRSIPSSTSSRAAAAPSFEKRWSRAPARSSSSSST 724
Query: 111 S 111
S
Sbjct: 725 S 727
>CK606481
Length = 541
Score = 33.5 bits (75), Expect = 0.092
Identities = 36/141 (25%), Positives = 49/141 (34%), Gaps = 2/141 (1%)
Frame = +3
Query: 81 PETKSDHKTPIQTPNREFSTPAKGKSEASTSYALRGVQQNQQGSLSLNWWSSPAKSGGEQ 140
P K KTP +R P K + A +G G + P K GG+Q
Sbjct: 93 PGKKPRKKTP---GDRGGGAPKNKKEIGAPGGATKGESPPPGGGKPGEFQRGPKKGGGKQ 263
Query: 141 EEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNSL--PAGNLNEEEWVTVYGFSPNDT 198
+KG G +T P P+ +PE +R + P G N W +G
Sbjct: 264 GQKG---------SRGENVTPPKFPQKKKPETKRGNPGGPKGGANPH*WGGAHGPLSKGE 416
Query: 199 NLVLREFEKCGEILKHVPGPR 219
N G +K P PR
Sbjct: 417 N-------GGGPRIKKTPNPR 458
>TC205645 UP|Q8RUD9 (Q8RUD9) Seed coat BURP domain protein 1, complete
Length = 1369
Score = 32.0 bits (71), Expect = 0.27
Identities = 24/64 (37%), Positives = 34/64 (52%), Gaps = 4/64 (6%)
Frame = +3
Query: 82 ETKSDHKT--PIQT-PNREFSTPAKGKSEASTSYALRGVQQNQQGSLSLNWWSSP-AKSG 137
E + HK+ P+ T PN E +T + EAS A RG + Q+ S + WS P A +G
Sbjct: 357 EVRQQHKSHSPVATAPNFETNTVLRR*KEASVGDAWRGSELKQRQDHSGDHWSLPRACNG 536
Query: 138 GEQE 141
G +E
Sbjct: 537 GRKE 548
>TC214758 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate
isomerase (Phosphoriboisomerase) , partial (79%)
Length = 836
Score = 30.8 bits (68), Expect = 0.59
Identities = 23/75 (30%), Positives = 31/75 (40%), Gaps = 2/75 (2%)
Frame = +3
Query: 33 SSPGQSGASSSLWRENFGNSDLPPP--PFFTLEDRSDFSPESGILDYQMSPETKSDHKTP 90
S P + ASSS + +S+ PPP P SP S +SP T TP
Sbjct: 267 SMPSTASASSSARASSRTSSESPPPQKPTNKPSPSGSPSPISTPTPPSISPSTAPTRSTP 446
Query: 91 IQTPNREFSTPAKGK 105
T +R + P+ K
Sbjct: 447 SSTSSRAVAAPSSEK 491
>TC218363 similar to UP|O73592 (O73592) CMIX, partial (11%)
Length = 627
Score = 30.4 bits (67), Expect = 0.78
Identities = 17/67 (25%), Positives = 28/67 (41%)
Frame = +2
Query: 119 QNQQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNSLP 178
+N++ S +WW+S + G RGS +P + PPP P + P
Sbjct: 47 KNEKWCSSASWWTSGGRCSGASLR--RGSAPAAAAEPAWPTSRPPPGSAMSPSTGSHGEP 220
Query: 179 AGNLNEE 185
+ L+ E
Sbjct: 221 SFALSAE 241
>TC211475 similar to UP|P79122 (P79122) Pinin, partial (5%)
Length = 436
Score = 30.0 bits (66), Expect = 1.0
Identities = 18/59 (30%), Positives = 25/59 (41%)
Frame = +2
Query: 118 QQNQQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNS 176
QQ QQ S + S GE+EE + + P + PPPP + P+ Q S
Sbjct: 113 QQQQQPSSQQHEEEEEEVSSGEEEEASSEEEEDENLPPPPISKNPPPPPPSNPQPQPTS 289
>TC222075 similar to UP|Q84XC0 (Q84XC0) Calcineurin B-like-interacting
protein kinase, partial (17%)
Length = 331
Score = 29.6 bits (65), Expect = 1.3
Identities = 21/58 (36%), Positives = 28/58 (48%)
Frame = -2
Query: 4 TAQRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPPPPFFT 61
T PRS + + ++ KF +PG +SSL +N NSDL PP FFT
Sbjct: 330 TGSERPRSREG*IGVDNCEEVADSSTIKFCNPG---FNSSL--QNLSNSDLSPPFFFT 172
>AW200869 similar to GP|16604527|gb| AT5g51550/K17N15_10 {Arabidopsis
thaliana}, partial (19%)
Length = 385
Score = 29.3 bits (64), Expect = 1.7
Identities = 15/35 (42%), Positives = 20/35 (56%)
Frame = +1
Query: 22 ASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPP 56
ASP + RR + SP +S +SSS W F + L P
Sbjct: 67 ASPPHRRRVRRFSPSRSSSSSSRWWARFRHGGLGP 171
>TC228613 weakly similar to UP|PE31_ARATH (Q9LHA7) Peroxidase 31 precursor
(Atperox P31) (ATP41) , partial (50%)
Length = 536
Score = 28.9 bits (63), Expect = 2.3
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = +3
Query: 155 PGALITLPPPPEVARPEVQRNSLPAGNLNEEEWVTVYGFSPNDT 198
P +T PPP+ P+ ++S A + ++ W++V G +P D+
Sbjct: 144 PPPFLTTSPPPQCPSPKSHKSSPTAASQSKNSWLSV-GPTPADS 272
>TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Protein C23),
partial (8%)
Length = 581
Score = 28.5 bits (62), Expect = 3.0
Identities = 23/78 (29%), Positives = 31/78 (39%), Gaps = 1/78 (1%)
Frame = -3
Query: 33 SSPGQSGASSSLWRENFGNSDLPPPPFFT-LEDRSDFSPESGILDYQMSPETKSDHKTPI 91
SSP S SSS + +S L PPP + S SP S SPE S +
Sbjct: 486 SSPSSSSLSSSSSSSSSSSSSLSPPPLASGSSSESASSPPS-------SPEKSSSSSSSS 328
Query: 92 QTPNREFSTPAKGKSEAS 109
+ F++ + S S
Sbjct: 327 SPSSPSFTSSSSSSSSVS 274
>TC208144 similar to UP|Q8VYU3 (Q8VYU3) GTP cyclohydrolase I , partial (28%)
Length = 1195
Score = 28.5 bits (62), Expect = 3.0
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -2
Query: 52 SDLPPPPFFTLEDRSDFSPESGILDYQMSPETKSDHKTPIQTPNR 96
S L P F L D + S D ++P+T SDHK+ +T R
Sbjct: 717 SGLDEFPLFFLRDHAKDSRRQKDNDVTINPQTNSDHKSHTETKIR 583
>TC228906 similar to UP|Q6ID88 (Q6ID88) At3g26510, partial (48%)
Length = 927
Score = 28.5 bits (62), Expect = 3.0
Identities = 24/87 (27%), Positives = 37/87 (41%)
Frame = +2
Query: 79 MSPETKSDHKTPIQTPNREFSTPAKGKSEASTSYALRGVQQNQQGSLSLNWWSSPAKSGG 138
+SP T TP ++PN+ + P+K S +S+S S S + + SP SG
Sbjct: 440 LSPPTPPTPPTPPRSPNKFSTAPSKSASISSSS---------ASSSSSSSSYYSPTGSG- 589
Query: 139 EQEEKGRGSPVEGVVQPGALITLPPPP 165
E GR +V + + PP
Sbjct: 590 ---ESGRRYRAPALVSDCCVHQMSQPP 661
>TC216068 similar to UP|Q9ZU51 (Q9ZU51) RING-H2 finger protein RHA2b, partial
(22%)
Length = 1060
Score = 28.1 bits (61), Expect = 3.9
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = -1
Query: 95 NREFSTPAKGKSEASTSYALRGVQQNQQGSLSLNWWSSPAK 135
N+E S + +S ++T + + ++ Q G L+L WWS P+K
Sbjct: 592 NQEMSCWPRRRSVSATRSSEKSGER-QSGQLNL*WWSQPSK 473
>BG507959 homologue to GP|3287690|gb|A T22J18.15 {Arabidopsis thaliana},
partial (7%)
Length = 257
Score = 28.1 bits (61), Expect = 3.9
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = -1
Query: 109 STSYALRGVQQNQQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGA 157
S S+ L G + G+LSL +W++P + + G P V + GA
Sbjct: 254 SVSFGLPGAFSQENGTLSLVFWTAPTEYDEHEVSATFGLPRRRVDKRGA 108
>TC206965
Length = 574
Score = 28.1 bits (61), Expect = 3.9
Identities = 14/42 (33%), Positives = 19/42 (44%)
Frame = +2
Query: 70 PESGILDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEASTS 111
P SG ++P T+S + I P STP +STS
Sbjct: 410 PRSGSTSSPLAPTTRSTSSSAIPEPEPSTSTPGSDARSSSTS 535
>TC216067 weakly similar to UP|RH2A_ARATH (Q9ZT50) RHA2a protein (RING-H2
zinc finger protein), partial (37%)
Length = 733
Score = 28.1 bits (61), Expect = 3.9
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = -1
Query: 95 NREFSTPAKGKSEASTSYALRGVQQNQQGSLSLNWWSSPAK 135
N+E S + +S ++T + + ++ Q G L+L WWS P+K
Sbjct: 565 NQEMSCWPRRRSVSATRSSEKSGER-QSGQLNL*WWSQPSK 446
>BE661546
Length = 624
Score = 26.9 bits (58), Expect = 8.6
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = +1
Query: 19 QDLASPVNARRGKFSSPGQSGASSSLWRENFG 50
Q+LA RR P Q+ SSS WRE++G
Sbjct: 514 QNLAXATTXRRA--GRPSQAVKSSSEWREDYG 603
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.129 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,611,938
Number of Sequences: 63676
Number of extensions: 179028
Number of successful extensions: 977
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 967
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 974
length of query: 247
length of database: 12,639,632
effective HSP length: 95
effective length of query: 152
effective length of database: 6,590,412
effective search space: 1001742624
effective search space used: 1001742624
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC138130.21