BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137986.3 + phase: 0
(571 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217577 similar to UP|Q9ASS7 (Q9ASS7) AT5g36940/MLF18_60, parti... 308 e-116
CA851850 weakly similar to PIR|T48473|T48 amino acid transport-l... 103 2e-22
BE803346 96 3e-20
TC234503 similar to PIR|T48473|T48473 amino acid transport-like ... 95 7e-20
BG507654 95 1e-19
TC212218 similar to PIR|T48473|T48473 amino acid transport-like ... 94 2e-19
AI900596 weakly similar to GP|13605811|gb| AT5g36940/MLF18_60 {A... 89 5e-18
BE608463 similar to PIR|T48473|T48 amino acid transport-like pro... 84 2e-16
TC218047 similar to UP|Q8GT81 (Q8GT81) Fiber protein Fb12 (Fragm... 81 1e-15
CO981042 79 7e-15
BF009685 65 8e-11
BF068630 61 1e-09
BI973511 39 4e-08
TC209179 similar to PIR|T48473|T48473 amino acid transport-like ... 52 7e-07
AW707189 32 0.77
TC232970 similar to PIR|T48473|T48473 amino acid transport-like ... 31 1.3
AW350457 30 2.2
TC205189 similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-like p... 30 2.9
>TC217577 similar to UP|Q9ASS7 (Q9ASS7) AT5g36940/MLF18_60, partial (44%)
Length = 1475
Score = 308 bits (790), Expect(2) = e-116
Identities = 153/189 (80%), Positives = 173/189 (90%)
Frame = +3
Query: 248 VGFDAVASTAEEVKNPQRDLPLGIGASLFLCCGMYMLVSVVVVGLVPYYAIDPDTPISSA 307
+GFDAVASTAEEVKNPQRDLPLGIG SLFLCCG+YMLVS+V+VGLVPYYAI+PDTPISSA
Sbjct: 6 IGFDAVASTAEEVKNPQRDLPLGIGGSLFLCCGLYMLVSIVIVGLVPYYAINPDTPISSA 185
Query: 308 FSDHGMQWAAYIINAGACTALISALMGGILPQPRILMAMARDGLLPPFFCDINKHTLVPV 367
F+D+GMQWAAY+IN GA TAL ++LMGGILPQPRILMAMARDGLLPPFF DINK + VPV
Sbjct: 186 FADNGMQWAAYVINGGAFTALCASLMGGILPQPRILMAMARDGLLPPFFSDINKCSQVPV 365
Query: 368 KGTIITGLAAAVLAFSMEVSDLAGMVSVGTLLAFTMVAISVLILRYIPPNKVPVPPSLQD 427
K TI+TGL A++LAFSMEVS+LAGMVSVGTLLAFTMVAISVLILRYIPP++V + PSLQ+
Sbjct: 366 KSTIVTGLVASLLAFSMEVSELAGMVSVGTLLAFTMVAISVLILRYIPPDEVLLLPSLQE 545
Query: 428 SIVEIKVEY 436
IV +Y
Sbjct: 546 PIVSASTQY 572
Score = 130 bits (327), Expect(2) = e-116
Identities = 65/100 (65%), Positives = 75/100 (75%), Gaps = 3/100 (3%)
Frame = +1
Query: 448 KPLDVTRDFSIDYPLISKHRSKGNHL---NRRRVVGWTIAIICLGAFVLTYAASCLTFLS 504
KP D ID PLI+K G ++ NRR+V+GW IA CLG FVLT+AAS T +S
Sbjct: 640 KPTVFKEDVPIDCPLIAKDPVIGKYVHEGNRRKVIGWVIAFTCLGVFVLTFAASNKTMIS 819
Query: 505 SVRFALCGVGGTLLVSGFVFLTCMDQDDARHDFGHSGGMS 544
SVRFALCGVGG LL+SGFVFLTCMDQDDARH+FGHSGG +
Sbjct: 820 SVRFALCGVGGCLLLSGFVFLTCMDQDDARHNFGHSGGFT 939
>CA851850 weakly similar to PIR|T48473|T48 amino acid transport-like protein
- Arabidopsis thaliana, partial (20%)
Length = 617
Score = 103 bits (258), Expect = 2e-22
Identities = 52/137 (37%), Positives = 84/137 (60%)
Frame = +3
Query: 12 ILFFTRRKQVDSPRQNTKPLLAKELSVYHLIAIGVGSTIGAGVYVLVGTVAREHSGPALA 71
++ +T ++ + R + + ++L + L+A+GVG +G GV+V G+VA HSGP++
Sbjct: 180 LVTWTPEQEFNQVRHRSGADMKRKLKWHDLVALGVGGMLGVGVFVTTGSVALHHSGPSVF 359
Query: 72 ISFLIAGLAAGLSAFCYAELACRCPSAGSAYHYSYICLGEGVAWLIGWALLLEYTIGSAA 131
IS++IAG++A LS+ CY E A + P AG A+ Y + GE + + G +L+EY +AA
Sbjct: 360 ISYIIAGISALLSSLCYTEFAVQVPVAGGAFSYLRLTFGEFLGYFAGANILMEYVFSNAA 539
Query: 132 VARGITPNLAPLFGGLD 148
VAR T L+ FG D
Sbjct: 540 VARSFTEYLSIAFGEND 590
>BE803346
Length = 437
Score = 96.3 bits (238), Expect = 3e-20
Identities = 41/58 (70%), Positives = 51/58 (87%)
Frame = +3
Query: 259 EVKNPQRDLPLGIGASLFLCCGMYMLVSVVVVGLVPYYAIDPDTPISSAFSDHGMQWA 316
+VKNPQRDLP+GI +L +CC +YMLV+ V+VGLVPYY ++PDTPISSAFS +GMQWA
Sbjct: 18 QVKNPQRDLPIGISTALTICCVLYMLVAAVIVGLVPYYELNPDTPISSAFSSYGMQWA 191
Score = 66.6 bits (161), Expect = 3e-11
Identities = 43/113 (38%), Positives = 59/113 (52%), Gaps = 8/113 (7%)
Frame = +1
Query: 261 KNPQRDLPLGIGASLFLCCG--------MYMLVSVVVVGLVPYYAIDPDTPISSAFSDHG 312
++PQ L +G +CCG YM VS ++ + + SD
Sbjct: 151 QSPQHFLAMGCNGQCKVCCGPLFNCLGYKYMDVS*ILCFKLHFT------------SDR- 291
Query: 313 MQWAAYIINAGACTALISALMGGILPQPRILMAMARDGLLPPFFCDINKHTLV 365
YII GA TAL ++L+G +LPQPR+ MAMARDGLLP FF +I+K T +
Sbjct: 292 -----YIITTGAVTALFASLLGSVLPQPRVFMAMARDGLLPHFFSNIHKGTQI 435
>TC234503 similar to PIR|T48473|T48473 amino acid transport-like protein -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(24%)
Length = 424
Score = 95.1 bits (235), Expect = 7e-20
Identities = 48/143 (33%), Positives = 85/143 (58%), Gaps = 8/143 (5%)
Frame = +1
Query: 205 FVIVAGGYMGFKSGWVGY------ELPTGYFPFGVDGMLAGSATVFFAYVGFDAVASTAE 258
FVI+ G + G W + P+G+FP G G+ G+A V+ +Y+G+DAV++ AE
Sbjct: 1 FVIMMGFWRG---NWKNFTEPANPHNPSGFFPHGAAGVFKGAALVYLSYIGYDAVSTMAE 171
Query: 259 EVKNPQRDLPLGIGASLFLCCGMYMLVSVVVVGLVPYYAIDPDTPISSAFS--DHGMQWA 316
EV++P +D+P+G+ S+ + +Y L++ + L+PY I+ + P S+AFS G W
Sbjct: 172 EVRDPVKDIPVGVSGSVVVVTVLYCLMAASMTKLLPYDVINAEAPFSAAFSGRSDGWGWV 351
Query: 317 AYIINAGACTALISALMGGILPQ 339
+ +I GA ++++L+ +L Q
Sbjct: 352 SRVIGVGASFGILTSLLVAMLGQ 420
>BG507654
Length = 411
Score = 94.7 bits (234), Expect = 1e-19
Identities = 49/139 (35%), Positives = 82/139 (58%)
Frame = +3
Query: 195 VTSVNVCALLFVIVAGGYMGFKSGWVGYELPTGYFPFGVDGMLAGSATVFFAYVGFDAVA 254
+T+ +V F+I+AG G V P G PFG G+L G+A V+F+Y+G+D+ +
Sbjct: 3 MTAFHVIFFGFIIIAGYCNGSAKNLVS---PKGLAPFGARGVLDGAAIVYFSYIGYDSAS 173
Query: 255 STAEEVKNPQRDLPLGIGASLFLCCGMYMLVSVVVVGLVPYYAIDPDTPISSAFSDHGMQ 314
+ AEEVK+P + LP+GI S+ + +Y L+++ + +VPY I S AF G
Sbjct: 174 TMAEEVKDPFKSLPIGIVGSVLITTLLYCLMALSLCMMVPYNKISEKASFSIAFLKIGWN 353
Query: 315 WAAYIINAGACTALISALM 333
WA+ ++ AGA ++++L+
Sbjct: 354 WASNLVGAGASLGIVASLL 410
>TC212218 similar to PIR|T48473|T48473 amino acid transport-like protein -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(22%)
Length = 401
Score = 93.6 bits (231), Expect = 2e-19
Identities = 41/111 (36%), Positives = 73/111 (64%), Gaps = 2/111 (1%)
Frame = +1
Query: 225 PTGYFPFGVDGMLAGSATVFFAYVGFDAVASTAEEVKNPQRDLPLGIGASLFLCCGMYML 284
PTG+FP G G+ G+A V+ +Y+G+DAV++ AEEV++P +D+P+G+ S+ + +Y L
Sbjct: 64 PTGFFPHGAAGVFKGAAAVYLSYIGYDAVSTMAEEVRDPVKDIPVGVSGSVVIVTVLYCL 243
Query: 285 VSVVVVGLVPYYAIDPDTPISSAFS--DHGMQWAAYIINAGACTALISALM 333
++ + L+PY I+ + P S+AFS G W + +I GA ++++L+
Sbjct: 244 MAASMTKLLPYDMINAEAPFSAAFSGRSDGWGWISGVIGVGASFGILTSLL 396
>AI900596 weakly similar to GP|13605811|gb| AT5g36940/MLF18_60 {Arabidopsis
thaliana}, partial (10%)
Length = 259
Score = 89.0 bits (219), Expect = 5e-18
Identities = 51/86 (59%), Positives = 63/86 (72%)
Frame = +2
Query: 328 LISALMGGILPQPRILMAMARDGLLPPFFCDINKHTLVPVKGTIITGLAAAVLAFSMEVS 387
L ++LMG IL ILMAMA DGLL P F DI L+PV T+++GL ++LAFSM VS
Sbjct: 2 LCASLMGVILSHS*ILMAMAMDGLLSPLFFDIY*SILLPV*STLVSGLVFSLLAFSMVVS 181
Query: 388 DLAGMVSVGTLLAFTMVAISVLILRY 413
+L+ MVSV TLLAFTMVA+ +LIL Y
Sbjct: 182 ELSSMVSVCTLLAFTMVALFLLILTY 259
>BE608463 similar to PIR|T48473|T48 amino acid transport-like protein -
Arabidopsis thaliana, partial (25%)
Length = 463
Score = 84.0 bits (206), Expect = 2e-16
Identities = 47/128 (36%), Positives = 70/128 (53%)
Frame = +2
Query: 16 TRRKQVDSPRQNTKPLLAKELSVYHLIAIGVGSTIGAGVYVLVGTVAREHSGPALAISFL 75
T ++ R + + K L + L+ + +G +GAGV+V G R ++GPA+ +S+
Sbjct: 80 TSYDEMSQVRSRSGTSMRKTLRWFDLVGLSIGGMVGAGVFVTTGHATR-YAGPAVLLSYA 256
Query: 76 IAGLAAGLSAFCYAELACRCPSAGSAYHYSYICLGEGVAWLIGWALLLEYTIGSAAVARG 135
IAG A LS FCY E A P AG A+ Y I GE A+L G L+ +Y + +AAVA
Sbjct: 257 IAGFCALLSGFCYTEFAVDMPVAGGAFSYLRITCGEYAAFLTGANLVEDYVLSNAAVAIS 436
Query: 136 ITPNLAPL 143
T +L +
Sbjct: 437 WTGDLGTI 460
>TC218047 similar to UP|Q8GT81 (Q8GT81) Fiber protein Fb12 (Fragment),
partial (91%)
Length = 833
Score = 81.3 bits (199), Expect = 1e-15
Identities = 39/89 (43%), Positives = 55/89 (60%), Gaps = 2/89 (2%)
Frame = +1
Query: 458 IDYPLISKHRSKG--NHLNRRRVVGWTIAIICLGAFVLTYAASCLTFLSSVRFALCGVGG 515
+ +PLI K +K N RR++ WTIA++C+G ++T AAS +R LCG+GG
Sbjct: 73 LGHPLIIKEVTKDEQNEKARRKLASWTIALLCIGILIVTSAASADWCPRILRLTLCGMGG 252
Query: 516 TLLVSGFVFLTCMDQDDARHDFGHSGGMS 544
LL+ + L C+ QDD RH FGHSGG +
Sbjct: 253 ILLLCSTIVLACVKQDDTRHSFGHSGGFA 339
>CO981042
Length = 666
Score = 78.6 bits (192), Expect = 7e-15
Identities = 37/96 (38%), Positives = 61/96 (63%)
Frame = +2
Query: 15 FTRRKQVDSPRQNTKPLLAKELSVYHLIAIGVGSTIGAGVYVLVGTVAREHSGPALAISF 74
+T ++ + R + + ++L + L+A+GVG +G GV+V G+VA HSGP++ IS+
Sbjct: 164 WTPDQEFNQVRHRSGADMKRKLKWHDLVALGVGGMLGVGVFVTTGSVALHHSGPSVFISY 343
Query: 75 LIAGLAAGLSAFCYAELACRCPSAGSAYHYSYICLG 110
+IAG++A LS+ CY E A + P AG A+ Y + G
Sbjct: 344 IIAGISALLSSLCYTEFAVQVPVAGGAFSYLRLTFG 451
>BF009685
Length = 169
Score = 65.1 bits (157), Expect = 8e-11
Identities = 31/56 (55%), Positives = 40/56 (71%)
Frame = +2
Query: 261 KNPQRDLPLGIGASLFLCCGMYMLVSVVVVGLVPYYAIDPDTPISSAFSDHGMQWA 316
+NP RDLP+GI + +CC + MLVS +VGL PY ++ DTPI SAFS + MQWA
Sbjct: 2 RNPDRDLPVGI*IA*TICCILSMLVSADLVGLHPYDKLNSDTPICSAFSTYAMQWA 169
>BF068630
Length = 336
Score = 61.2 bits (147), Expect = 1e-09
Identities = 30/41 (73%), Positives = 36/41 (87%)
Frame = +2
Query: 389 LAGMVSVGTLLAFTMVAISVLILRYIPPNKVPVPPSLQDSI 429
LAGMVSVGTLLAFT VA+SVLI+RY+PP++VPV SL S+
Sbjct: 5 LAGMVSVGTLLAFTTVAVSVLIIRYVPPDEVPVLSSLLTSV 127
>BI973511
Length = 421
Score = 38.5 bits (88), Expect(2) = 4e-08
Identities = 20/56 (35%), Positives = 30/56 (52%)
Frame = +2
Query: 198 VNVCALLFVIVAGGYMGFKSGWVGYELPTGYFPFGVDGMLAGSATVFFAYVGFDAV 253
+ + ++ VI AG + S W + + P G+ + G+ VFFAYVGFDAV
Sbjct: 272 LQIIIVIIVIFAGAFEVDVSNW------SPFAPNGLKAIFTGATVVFFAYVGFDAV 421
Score = 37.4 bits (85), Expect(2) = 4e-08
Identities = 18/53 (33%), Positives = 33/53 (61%)
Frame = +1
Query: 148 DNLPFFLSRQHIPGIDIVVDPCAAILVLLVTGLLCLGIKESTVVQGIVTSVNV 200
DN+P ++ G + ++ A IL++L+T +LC G++ES+VV ++T V
Sbjct: 10 DNIPKWIGHGEDIGDVLSINVLAPILLVLLTFILCRGVQESSVVNSLMTVTKV 168
>TC209179 similar to PIR|T48473|T48473 amino acid transport-like protein -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(36%)
Length = 921
Score = 52.0 bits (123), Expect = 7e-07
Identities = 27/96 (28%), Positives = 49/96 (50%)
Frame = +1
Query: 330 SALMGGILPQPRILMAMARDGLLPPFFCDINKHTLVPVKGTIITGLAAAVLAFSMEVSDL 389
++L+ +L Q R + + R ++P +F ++ T PV + G+ A +A ++ L
Sbjct: 1 ASLLVAMLGQARYMCVIGRSNVVPSWFARVHPKTSTPVNASAFLGIFTAAIALFTDLDVL 180
Query: 390 AGMVSVGTLLAFTMVAISVLILRYIPPNKVPVPPSL 425
+V +GTL F MVA +V+ RY+ P+L
Sbjct: 181 FNLVCIGTLFVFYMVANAVIYRRYVATGTTNPWPTL 288
>AW707189
Length = 442
Score = 32.0 bits (71), Expect = 0.77
Identities = 26/78 (33%), Positives = 40/78 (50%), Gaps = 2/78 (2%)
Frame = +2
Query: 149 NLPFFLSRQHIPGIDIVVDPCAAILVLLVTG--LLCLGIKESTVVQGIVTSVNVCALLFV 206
+L F+ S GI +V IL++L G LL G K S + T++ + L FV
Sbjct: 164 SLAFYYSSAMAIGIILV------ILIILYQGMKLLPTGRKSSLAIFLYSTALAIFLLTFV 325
Query: 207 IVAGGYMGFKSGWVGYEL 224
+AG ++GF WV ++L
Sbjct: 326 AIAGAWLGF---WVVHKL 370
>TC232970 similar to PIR|T48473|T48473 amino acid transport-like protein -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(9%)
Length = 888
Score = 31.2 bits (69), Expect = 1.3
Identities = 14/43 (32%), Positives = 25/43 (57%)
Frame = +3
Query: 378 AVLAFSMEVSDLAGMVSVGTLLAFTMVAISVLILRYIPPNKVP 420
A + E+ + ++S+GTLL F MVA +++ RY+ + P
Sbjct: 3 ATITLFTELDIIIELISIGTLLVFYMVANALIYRRYVITSHAP 131
>AW350457
Length = 458
Score = 30.4 bits (67), Expect = 2.2
Identities = 18/70 (25%), Positives = 36/70 (50%), Gaps = 2/70 (2%)
Frame = +2
Query: 393 VSVGTLLAFTMVAISVLILRYIPPNKVPVPPSLQDSIVEIKVEYEEENIGTSE--VSKPL 450
+S +LL V + + L P +P+P S +D E + E EEE++ + ++KPL
Sbjct: 218 LSEASLLEDPFVVNAFIGLAKAQPQLLPLPSSTEDETQEEEEEEEEEDVVDEDEVITKPL 397
Query: 451 DVTRDFSIDY 460
++ ++ +
Sbjct: 398 NIPPHLTLSF 427
>TC205189 similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-like protein,
partial (91%)
Length = 1715
Score = 30.0 bits (66), Expect = 2.9
Identities = 35/153 (22%), Positives = 62/153 (39%), Gaps = 4/153 (2%)
Frame = +1
Query: 284 LVSVVVVGLVPYYAIDPDTPISSAFSDHGMQWAAYIINAGACTALISALMGGILPQPRIL 343
L+ +G V Y DP I A ++ G+ I GA I +L P
Sbjct: 265 LLKSTTIGKVRLYGADP--AIIKALANSGIG-----IVIGAANGDIPSLAA----DPNAA 411
Query: 344 MAMARDGLLPPFFCDINKHTLVPVKGTIIT----GLAAAVLAFSMEVSDLAGMVSVGTLL 399
+LP + + TL+ V I+T GL + ++ V + G S+G +
Sbjct: 412 TQWVNANVLP--YYPASNITLITVGNEILTLADQGLKSQLVPAMRNVQNALGAASLGGKI 585
Query: 400 AFTMVAISVLILRYIPPNKVPVPPSLQDSIVEI 432
+ V ++ + PP+ P+LQD++ ++
Sbjct: 586 KVSTVHSMAVLTQSDPPSSGLFNPALQDTLKQL 684
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.143 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,144,021
Number of Sequences: 63676
Number of extensions: 431288
Number of successful extensions: 2794
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 2760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2793
length of query: 571
length of database: 12,639,632
effective HSP length: 102
effective length of query: 469
effective length of database: 6,144,680
effective search space: 2881854920
effective search space used: 2881854920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC137986.3