BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137669.1 - phase: 0
(284 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208920 weakly similar to GB|AAT42380.1|48427662|BT014888 At4g3... 158 3e-39
CA851986 weakly similar to GP|13365694|dbj caffeine synthase {Co... 154 6e-38
TC218210 weakly similar to UP|Q84PP8 (Q84PP8) 3,7-dimethylxanthi... 150 8e-37
TC231316 similar to UP|Q9FLN8 (Q9FLN8) S-adenosyl-L-methionine:s... 134 5e-32
BF595358 weakly similar to GP|13366161|dbj S-adenosyl-L-methioni... 114 7e-26
BF219584 weakly similar to GP|20271026|gb| N-methyltransferase {... 95 4e-20
BE555819 55 4e-08
BQ786620 weakly similar to GP|13235641|emb S-adenosyl-L-methioni... 53 1e-07
BI426827 weakly similar to GP|9758122|dbj| S-adenosyl-L-methioni... 51 7e-07
TC218211 weakly similar to UP|Q84PP8 (Q84PP8) 3,7-dimethylxanthi... 45 3e-05
TC221972 weakly similar to UP|Q8W414 (Q8W414) S-adenosyl-L-methi... 38 0.005
AW830710 35 0.030
TC207020 homologue to UP|Q9FQE5 (Q9FQE5) Glutathione S-transfera... 31 0.73
TC208995 similar to UP|Q9V551 (Q9V551) CG8026-PA, partial (6%) 30 1.6
TC226780 similar to UP|Q9T024 (Q9T024) DnaJ-like protein (AT4g39... 28 3.6
TC213107 28 3.6
BG652022 28 4.7
TC212145 UP|Q76IB9 (Q76IB9) Apyrase (Fragment), complete 27 8.1
TC208491 similar to UP|Q7XBI0 (Q7XBI0) Swi2/Snf2-related protein... 27 8.1
>TC208920 weakly similar to GB|AAT42380.1|48427662|BT014888 At4g36470
{Arabidopsis thaliana;} , partial (48%)
Length = 1028
Score = 158 bits (399), Expect = 3e-39
Identities = 79/168 (47%), Positives = 109/168 (64%), Gaps = 6/168 (3%)
Frame = +2
Query: 72 GPNTLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVE 131
GPNTL +I ++ + + Q H S E++V+ NDL NDFN+IF+ L F + L + +
Sbjct: 2 GPNTLSIIKDIFQAIQGTSQRIMHHSTEFRVYFNDLPTNDFNSIFKALPEFQKLLRQDRK 181
Query: 132 DGIGGPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVEN------NKGNIYM 185
+G IF G PGSFYGR+FP +HF+HSS+SL WLS+VP + + NKG +Y+
Sbjct: 182 NGFPS-IFMGGYPGSFYGRLFPNSYLHFVHSSFSLHWLSRVPPSLYDEHKRPLNKGCVYI 358
Query: 186 ATTSPANVLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSDD 233
+SP V AY++QFQ DFSLFL+ R+EELV GGRMVL GR+ +
Sbjct: 359 CESSPEVVSQAYYQQFQEDFSLFLRSRSEELVVGGRMVLIFLGRRGPE 502
>CA851986 weakly similar to GP|13365694|dbj caffeine synthase {Coffea
arabica}, partial (19%)
Length = 635
Score = 154 bits (388), Expect = 6e-38
Identities = 82/183 (44%), Positives = 114/183 (61%), Gaps = 6/183 (3%)
Frame = +1
Query: 11 MKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCS 70
M+ + +LHMN G GE SY+NNS+LQ+K++ K + +E I Y P + +ADLGCS
Sbjct: 88 MESKLLLHMNSGKGERSYANNSMLQRKLMIKGKHILEETITRFYSNYSPSCMKVADLGCS 267
Query: 71 SGPNTLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEV 130
GPNTLLVIS +I +VD C N E P +Q ++NDL GNDFN F+ L F ++L DE
Sbjct: 268 VGPNTLLVISNIIDIVDTTCTRLNQEPPTFQFYLNDLFGNDFNTTFKSLPDFYKRL-DED 444
Query: 131 EDGIGGPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVP-----KGVEN-NKGNIY 184
+ G F PGSF+GR+FP +++ HS+ SL WLSQ P K E+ NKG+ +
Sbjct: 445 KGHKFGSCFINATPGSFHGRLFPNNSINLFHSANSLHWLSQDPLLEFTKEAESFNKGHCH 624
Query: 185 MAT 187
+ +
Sbjct: 625 IVS 633
>TC218210 weakly similar to UP|Q84PP8 (Q84PP8) 3,7-dimethylxanthine
N-methyltransferase, partial (27%)
Length = 845
Score = 150 bits (378), Expect = 8e-37
Identities = 75/156 (48%), Positives = 100/156 (64%)
Frame = +1
Query: 16 VLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCSSGPNT 75
+LHMNGG GE SY+NN LLQKK++ K + +E I LY P + + +LGCS GPN
Sbjct: 19 LLHMNGGKGERSYTNNCLLQKKLMLKAKPILEETIMRLYRDFSPNCMKVTNLGCSVGPNA 198
Query: 76 LLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVEDGIG 135
LLVIS +I +V+ C N E P++Q ++NDL GN FN IF+ L F +L ++
Sbjct: 199 LLVISNIIDIVNTACTSLNREPPKFQFYLNDLFGNGFNTIFKSLPNFYTRLVEDKGHKF- 375
Query: 136 GPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQ 171
GP F PGSFYGR+FP+ +++ HSS SL WLSQ
Sbjct: 376 GPCFVNATPGSFYGRLFPSNSINLFHSSNSLHWLSQ 483
>TC231316 similar to UP|Q9FLN8 (Q9FLN8) S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase-like protein, partial
(76%)
Length = 1111
Score = 134 bits (337), Expect = 5e-32
Identities = 84/240 (35%), Positives = 129/240 (53%), Gaps = 15/240 (6%)
Frame = +1
Query: 9 TRMKVEQVLHMNGGIGETSYSNNSLLQ----KKVISLTKEMRD--EAIKNLYCKTFPKRL 62
+ M++E++L M GG GE SY+NNS Q + ++ L +E D E ++
Sbjct: 124 SNMELEKLLSMKGGKGEASYANNSQAQAIHARSMLHLLRETLDRVEVVEGREVAFV---- 291
Query: 63 GIADLGCSSGPNTLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLD-- 120
+ADLGCS G N++ V+ +IK + K + + PE+ F +DL NDFN +F+LL
Sbjct: 292 -VADLGCSCGSNSINVVDVMIKHMMKRYEALGWQPPEFSAFFSDLPSNDFNTLFQLLPPL 468
Query: 121 -RFTEKLNDEVEDGIGGPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVEN- 178
+ + + + F G PGSFY R+FP +++ HS++SL WLSQVP+ VE+
Sbjct: 469 ANYGVSMEECLAANNHRSYFAAGVPGSFYRRLFPARSVDVFHSAFSLHWLSQVPESVEDK 648
Query: 179 -----NKGNIYMATTSPANVLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSDD 233
NKG +++ + NAY +QFQ D + FL+ R+ E+ G M L R S D
Sbjct: 649 RSSAYNKGKVFIHGAGESTA-NAYKKQFQTDLAGFLRARSVEMKREGSMFLVCLARTSVD 825
>BF595358 weakly similar to GP|13366161|dbj S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase {Atropa belladonna},
partial (14%)
Length = 415
Score = 114 bits (284), Expect = 7e-26
Identities = 60/136 (44%), Positives = 84/136 (61%)
Frame = +2
Query: 16 VLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCSSGPNT 75
+LHMNGG GE SY+++S LQKK++ + +E I LY + P + +ADLGCS GPN+
Sbjct: 11 LLHMNGGKGERSYAHHSSLQKKLMLKATPILEETIMKLYHDSSPSCMKVADLGCSVGPNS 190
Query: 76 LLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVEDGIG 135
LLVIS +I +VD C N E P + ++NDL GNDFN IF+ L +L ++ +
Sbjct: 191 LLVISNIINIVDTTCTILNCEPPTFPFYLNDLLGNDFNTIFQSLPDSHTRLGED*GHKL- 367
Query: 136 GPIFFYGAPGSFYGRI 151
G F PGS YG++
Sbjct: 368 GLCFINATPGSCYGKV 415
>BF219584 weakly similar to GP|20271026|gb| N-methyltransferase {Coffea
canephora}, partial (18%)
Length = 597
Score = 94.7 bits (234), Expect = 4e-20
Identities = 51/107 (47%), Positives = 62/107 (57%), Gaps = 6/107 (5%)
Frame = +3
Query: 136 GPIFFYGAPGSFYGRIFPTKTMHFIHSSYSLQWLSQVP------KGVENNKGNIYMATTS 189
GP F PGS+YGR+FP+ ++F HSS SL WLSQ P + N GN Y+ +TS
Sbjct: 75 GPCFINATPGSYYGRLFPSNFINFFHSSTSLHWLSQDPLLGLTREAESLN*GNCYIVSTS 254
Query: 190 PANVLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGRKSDDKYS 236
P V AY +Q Q F LFLK R EEL+ GG MVL GR + S
Sbjct: 255 PPEVYKAYLKQSQEGFKLFLKSRWEELMPGGAMVLVFPGRNETPRRS 395
>BE555819
Length = 425
Score = 55.1 bits (131), Expect = 4e-08
Identities = 25/45 (55%), Positives = 33/45 (72%)
Frame = +2
Query: 184 YMATTSPANVLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWG 228
++ +TSP + AY +QFQ+DF LFLK R+EELV GG MVL + G
Sbjct: 23 HIVSTSPPEIYKAYVKQFQQDFKLFLKSRSEELVPGGAMVLVVLG 157
>BQ786620 weakly similar to GP|13235641|emb S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase {Stephanotis
floribunda}, partial (21%)
Length = 429
Score = 53.1 bits (126), Expect = 1e-07
Identities = 24/42 (57%), Positives = 30/42 (71%)
Frame = +2
Query: 179 NKGNIYMATTSPANVLNAYHEQFQRDFSLFLKCRAEELVDGG 220
NKGN ++ +TSP+ V AY +QFQ F FLK R+EELV GG
Sbjct: 302 NKGNCHLVSTSPSEVYKAYFKQFQEGFKSFLKSRSEELVPGG 427
Score = 41.2 bits (95), Expect = 5e-04
Identities = 20/40 (50%), Positives = 26/40 (65%)
Frame = +3
Query: 14 EQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNL 53
E +LHMNGG GE SY+N S QKK++ K + +E I L
Sbjct: 144 ELLLHMNGGKGERSYANYSSFQKKLMLKAKSILEETITTL 263
>BI426827 weakly similar to GP|9758122|dbj| S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase-like protein, partial
(23%)
Length = 422
Score = 50.8 bits (120), Expect = 7e-07
Identities = 28/80 (35%), Positives = 43/80 (53%)
Frame = +3
Query: 9 TRMKVEQVLHMNGGIGETSYSNNSLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIADLG 68
+ M++E++L M GG GE SY+NNS Q + EA+ + + + DLG
Sbjct: 162 SNMELERLLSMKGGKGEGSYANNSQAQAIHAKSMHHLLKEALDGVQLQAPNMPFVVVDLG 341
Query: 69 CSSGPNTLLVISEVIKLVDK 88
CS G NT+ V+ +IK + K
Sbjct: 342 CSCGSNTINVVDLIIKHIIK 401
>TC218211 weakly similar to UP|Q84PP8 (Q84PP8) 3,7-dimethylxanthine
N-methyltransferase, partial (17%)
Length = 645
Score = 45.4 bits (106), Expect = 3e-05
Identities = 18/28 (64%), Positives = 23/28 (81%)
Frame = +2
Query: 144 PGSFYGRIFPTKTMHFIHSSYSLQWLSQ 171
PGSFYGR+FP+ +++ HSS SL WLSQ
Sbjct: 8 PGSFYGRLFPSNSINLFHSSNSLHWLSQ 91
>TC221972 weakly similar to UP|Q8W414 (Q8W414)
S-adenosyl-L-methionine:salicylic acid calboxyl
methyltransferase-like protein, partial (9%)
Length = 702
Score = 38.1 bits (87), Expect = 0.005
Identities = 21/37 (56%), Positives = 23/37 (61%)
Frame = +2
Query: 193 VLNAYHEQFQRDFSLFLKCRAEELVDGGRMVLTIWGR 229
V A QF F+LFLK RAEEL+ GG MVL GR
Sbjct: 5 VYQAXXXQFSPYFNLFLKSRAEELLRGGGMVLRFVGR 115
>AW830710
Length = 137
Score = 35.4 bits (80), Expect = 0.030
Identities = 17/29 (58%), Positives = 23/29 (78%), Gaps = 1/29 (3%)
Frame = +1
Query: 11 MKVEQV-LHMNGGIGETSYSNNSLLQKKV 38
M+ EQ+ LHMNGG G+ SY+NNS LQ+ +
Sbjct: 31 MEREQLSLHMNGGKGQRSYANNSSLQRTI 117
>TC207020 homologue to UP|Q9FQE5 (Q9FQE5) Glutathione S-transferase GST 13 ,
partial (97%)
Length = 1350
Score = 30.8 bits (68), Expect = 0.73
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = -1
Query: 161 HSSYSLQWLSQVPKGVENNKGNIYMATTSPANVLNAYHEQ 200
HS L W + V K N + ++ AN LN YH Q
Sbjct: 1080 HSGNFLAWFNFVEKSGNNTEHQFFIVKIKIANTLNQYHSQ 961
>TC208995 similar to UP|Q9V551 (Q9V551) CG8026-PA, partial (6%)
Length = 1025
Score = 29.6 bits (65), Expect = 1.6
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 4/37 (10%)
Frame = -3
Query: 137 PIFFYGAPGSFYG----RIFPTKTMHFIHSSYSLQWL 169
P+FFY SF G R+F T+ + S YSL+W+
Sbjct: 417 PLFFYNLAHSFKGPLTPRVFLRITLGLLASVYSLKWV 307
>TC226780 similar to UP|Q9T024 (Q9T024) DnaJ-like protein
(AT4g39150/T22F8_50), partial (95%)
Length = 1349
Score = 28.5 bits (62), Expect = 3.6
Identities = 20/94 (21%), Positives = 39/94 (41%)
Frame = +1
Query: 144 PGSFYGRIFPTKTMHFIHSSYSLQWLSQVPKGVENNKGNIYMATTSPANVLNAYHEQFQR 203
P + +G IF ++ +L L+ + +E + + + + A+ ++ ++
Sbjct: 373 PTTVFGMIFGSEFFEEYIGQLALASLASIE--IEEDSQDPEVLRQRIQEKMKAWQKEREQ 546
Query: 204 DFSLFLKCRAEELVDGGRMVLTIWGRKSDDKYSK 237
S FLK R + VDG T W + SK
Sbjct: 547 KLSTFLKDRLQPFVDGREDEFTAWAKSEARSLSK 648
>TC213107
Length = 840
Score = 28.5 bits (62), Expect = 3.6
Identities = 19/61 (31%), Positives = 32/61 (52%)
Frame = -3
Query: 36 KKVISLTKEMRDEAIKNLYCKTFPKRLGIADLGCSSGPNTLLVISEVIKLVDKLCQEHNH 95
K ++ +TK + DE IK+ Y T L + +GC S +T + + ++ KL EH H
Sbjct: 502 KVLVHVTKILTDENIKDGYPNTKN*LLTLT-VGCQSVVSTESIYIHQLSVLPKLPVEHYH 326
Query: 96 E 96
+
Sbjct: 325 Q 323
>BG652022
Length = 424
Score = 28.1 bits (61), Expect = 4.7
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +1
Query: 75 TLLVISEVIKLVDKLCQEHNHESPEYQVFMNDLQGNDFNNIF 116
TLL++ E++ L L Q+H ++P Q+ +GND N++
Sbjct: 229 TLLILQEIVALTLPLLQQHQQQAPILQLK*TG-EGNDRCNVW 351
>TC212145 UP|Q76IB9 (Q76IB9) Apyrase (Fragment), complete
Length = 1173
Score = 27.3 bits (59), Expect = 8.1
Identities = 25/83 (30%), Positives = 39/83 (46%), Gaps = 11/83 (13%)
Frame = +2
Query: 16 VLHMNGGI--------GETSYSNN-SLLQKKVISLTKEMRDEAIKNLYCKTFPKRLGIAD 66
+LH+ G+ G +SY+NN K +I L ++ D +L KT P RLGI
Sbjct: 50 LLHIGKGVEYYNKITPGLSSYANNPEQAAKSLIPLLEQAEDVVPDDLQPKT-PVRLGILQ 226
Query: 67 LG--CSSGPNTLLVISEVIKLVD 87
G S +T V + + ++D
Sbjct: 227 SGRDMLSNRSTFNVQPDAVSIID 295
>TC208491 similar to UP|Q7XBI0 (Q7XBI0) Swi2/Snf2-related protein DDM1,
partial (22%)
Length = 839
Score = 27.3 bits (59), Expect = 8.1
Identities = 18/70 (25%), Positives = 30/70 (42%)
Frame = +2
Query: 87 DKLCQEHNHESPEYQVFMNDLQGNDFNNIFRLLDRFTEKLNDEVEDGIGGPIFFYGAPGS 146
D L + E+ E ++ D+ D +LLDR +ND +D P+ + G
Sbjct: 380 DVLALLRDEETAEDKMIHTDISDEDLE---KLLDRSDLIVNDFNDDNFKAPVSVFPLKGP 550
Query: 147 FYGRIFPTKT 156
+ + PT T
Sbjct: 551 GWEVVIPTAT 580
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,348,775
Number of Sequences: 63676
Number of extensions: 152756
Number of successful extensions: 683
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 675
length of query: 284
length of database: 12,639,632
effective HSP length: 96
effective length of query: 188
effective length of database: 6,526,736
effective search space: 1227026368
effective search space used: 1227026368
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC137669.1