BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137603.8 - phase: 0 /pseudo
(676 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF070365 similar to PIR|T49914|T49 callose synthase catalytic su... 115 8e-26
AW831736 94 2e-19
TC234651 78 1e-14
BQ094797 76 4e-14
TC223646 45 3e-07
TC217685 similar to GB|AAM16165.1|20334818|AY094009 AT4g26750/F1... 49 6e-06
CA783198 similar to GP|15081654|gb AT4g26750/F10M23_90 {Arabidop... 42 7e-04
TC224160 34 0.18
TC226809 similar to GB|AAP37699.1|30725354|BT008340 At5g20700 {A... 31 1.6
TC203868 homologue to UP|O24482 (O24482) Sali3-2, partial (62%) 30 3.5
TC205555 30 3.5
TC221367 weakly similar to UP|Q84VY9 (Q84VY9) At1g30130, partial... 30 4.6
TC233081 29 7.8
TC206835 similar to GB|AAQ56832.1|34098899|BT010389 At2g34040 {A... 29 7.8
BU546627 similar to GP|11994328|dbj receptor protein-like {Arabi... 29 7.8
TC209529 29 7.8
TC208937 similar to GB|AAM70562.1|21700877|AY124853 At1g74910/F9... 29 7.8
>BF070365 similar to PIR|T49914|T49 callose synthase catalytic subunit-like
protein - Arabidopsis thaliana, partial (3%)
Length = 259
Score = 115 bits (287), Expect = 8e-26
Identities = 58/84 (69%), Positives = 64/84 (76%)
Frame = +2
Query: 17 PLQRRITRTQTAGNLGEAIFDSEVVPSSLVEIAPILRVANEVEKTHPRVAYLCRFYAFEK 76
P + I RTQTAGNLGE++ D EVVP +VEIAPILRV +EVEKTHP YLCR YA EK
Sbjct: 8 PSPKLIMRTQTAGNLGESVIDREVVPYYMVEIAPILRVTDEVEKTHPMDDYLCRIYASEK 187
Query: 77 AHRLDPTSSGRGVRQFKTALLQRL 100
AH +DP SGRGVRQF TALL L
Sbjct: 188 AHMVDPNISGRGVRQFTTALLHAL 259
>AW831736
Length = 396
Score = 94.0 bits (232), Expect = 2e-19
Identities = 49/117 (41%), Positives = 71/117 (59%), Gaps = 3/117 (2%)
Frame = +2
Query: 190 LVPYNILPLDPDSANQAIMRFPEIQAAVFALRNTRGLA-WPKDYK--KKKDEDILDWLGA 246
L PYNI+PL+ S I FPE++AA+ A+R T P +K ++D D+ D L
Sbjct: 44 LTPYNIIPLEAPSLTNPIRIFPEVKAAISAIRYTDQFPRLPAGFKISGQRDADMFDLLEF 223
Query: 247 MFGFQKHNVANQREHLILLLANVHIRQFPKPDQQPKLDERALTEVMKKLFKNYKKWC 303
+FGFQK NV NQRE+++L++AN R + PK+DE+ + EV K+ NY +WC
Sbjct: 224 VFGFQKDNVRNQRENVVLMIANKQSRLGIPAETDPKIDEKTINEVFLKVLDNYIRWC 394
>TC234651
Length = 601
Score = 78.2 bits (191), Expect = 1e-14
Identities = 33/36 (91%), Positives = 33/36 (91%)
Frame = +2
Query: 421 DLNEYFWSADCFRLGWPMRADADFFCLPVEHLHFDK 456
DLNEYFWSADCFRLGWPMRADADFFCLP E L FDK
Sbjct: 2 DLNEYFWSADCFRLGWPMRADADFFCLPAEKLVFDK 109
>BQ094797
Length = 423
Score = 76.3 bits (186), Expect = 4e-14
Identities = 44/63 (69%), Positives = 46/63 (72%)
Frame = +1
Query: 79 RLDPTSSGRGVRQFKTALLQRLERENDPTLKGRIKKSDAREMQSFYQHYYKKYIQALQNA 138
RLD SSG GV QFKTALL RLE EN T R K SDAREMQ+FY YY+KYIQAL A
Sbjct: 238 RLDLHSSGSGVCQFKTALLWRLENEN-LTTHVRRKTSDAREMQTFYGQYYEKYIQALDKA 414
Query: 139 ADK 141
ADK
Sbjct: 415 ADK 423
>TC223646
Length = 526
Score = 45.4 bits (106), Expect(2) = 3e-07
Identities = 19/23 (82%), Positives = 20/23 (86%)
Frame = +1
Query: 291 VMKKLFKNYKKWCKYLGRKSSLW 313
V KKLF+NYKKWC YLG KSSLW
Sbjct: 97 VKKKLFRNYKKWC*YLGCKSSLW 165
Score = 27.7 bits (60), Expect(2) = 3e-07
Identities = 18/44 (40%), Positives = 18/44 (40%)
Frame = +3
Query: 247 MFGFQKHNVANQREHLILLLANVHIRQFPKPDQQPKLDERALTE 290
MFGFQK NV N PK DQ K DE L E
Sbjct: 15 MFGFQKDNVEN-----------------PKSDQLLKFDESCLNE 95
>TC217685 similar to GB|AAM16165.1|20334818|AY094009 AT4g26750/F10M23_90
{Arabidopsis thaliana;} , partial (57%)
Length = 1586
Score = 49.3 bits (116), Expect = 6e-06
Identities = 33/126 (26%), Positives = 57/126 (45%), Gaps = 5/126 (3%)
Frame = +3
Query: 48 IAPILRVANEVEKTHPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLERENDPT 107
+ P L+ A+E++K P VAY CR YA E+ ++ + + +L+++LE++
Sbjct: 69 LLPYLQRADELQKHEPLVAYYCRLYAMERGLKIPQSERTKTTNALLVSLMKQLEKDKKSI 248
Query: 108 LKGRIKKSDAREMQSFYQHYYKKYIQALQNAADKADRA-----QLTKAYQTANVLFEVLK 162
G D ++ F + + K ADK DRA K + A++ FE+L
Sbjct: 249 QLG---PEDNLYLEGFALNVFGK--------ADKQDRAGRADLNTAKTFYAASIFFEILN 395
Query: 163 AVNMTQ 168
Q
Sbjct: 396 QFGAVQ 413
>CA783198 similar to GP|15081654|gb AT4g26750/F10M23_90 {Arabidopsis
thaliana}, partial (24%)
Length = 457
Score = 42.4 bits (98), Expect = 7e-04
Identities = 27/98 (27%), Positives = 47/98 (47%)
Frame = +1
Query: 48 IAPILRVANEVEKTHPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLERENDPT 107
+ P L+ A+E++K P VAY CR YA E+ ++ + + +L+++LE++
Sbjct: 52 LLPYLQRADELQKHEPLVAYYCRLYAMERGLKIPQSERTKTTNALLVSLMKQLEKDKKSI 231
Query: 108 LKGRIKKSDAREMQSFYQHYYKKYIQALQNAADKADRA 145
G D ++ F + + K ADK DRA
Sbjct: 232 QLG---PEDNLYLEGFALNVFGK--------ADKQDRA 312
>TC224160
Length = 932
Score = 34.3 bits (77), Expect = 0.18
Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 8/55 (14%)
Frame = +1
Query: 612 VVYLSPNMLAAILFLLYI--------DSNLVVLFPCT*QPRLYVGRGMHESTFSL 658
+++ S A+LFL + D ++V F QP +YVGRGMH+S F+L
Sbjct: 241 IIFASEFTSTAVLFLFPMLRRWIENSDWHIVRFFLWWSQPSIYVGRGMHDSQFAL 405
Score = 30.4 bits (67), Expect = 2.7
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +2
Query: 572 VIVLSVTYAYTWDNPPGFAQSIQSWFGSNSHSPSMFILAVVVYLSPNMLAAI 623
VI+L + Y ++ P + + S+ S ++LAV +YL PN+LA +
Sbjct: 119 VIILPLFYVHSLKGAPQGLKQLLSFSKQIRGFSSFYMLAVALYLLPNLLAQL 274
>TC226809 similar to GB|AAP37699.1|30725354|BT008340 At5g20700 {Arabidopsis
thaliana;} , partial (27%)
Length = 1148
Score = 31.2 bits (69), Expect = 1.6
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = -3
Query: 589 FAQSIQSWFGSNSHSPSMFILAVVVYLSPNMLAAILFLLYIDSNLVVLFPC 639
F + + WFGS+ + +F++ VVV LSPN + ++Y+ V C
Sbjct: 618 FGLNQRLWFGSSENYNVVFVV-VVVVLSPNNITFTTIIVYLSERFVRYAAC 469
>TC203868 homologue to UP|O24482 (O24482) Sali3-2, partial (62%)
Length = 528
Score = 30.0 bits (66), Expect = 3.5
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = +2
Query: 290 EVMKKLFKNYKKWCKYLGRKSSLWLPTIQQE 320
EV+ K+ N +C +LG K+ LW+P + +
Sbjct: 398 EVLCKILGNSNWFCHFLGSKAILWVPNLSMD 490
>TC205555
Length = 552
Score = 30.0 bits (66), Expect = 3.5
Identities = 23/89 (25%), Positives = 40/89 (44%), Gaps = 22/89 (24%)
Frame = +3
Query: 287 ALTEVMKKLFKNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYM--------GLYLLIWGE 338
ALT + KK+ +Y +C + + SSLW RKL+++ L LL++GE
Sbjct: 171 ALTVLTKKMSTSYIPFCASMVKVSSLW----------RKLIHVVDPT*WDKALLLLLYGE 320
Query: 339 AANLRFMPE--------------CLCYIY 353
+ + ++ + C+C IY
Sbjct: 321 SVMMSYLMQICSMVEFLQIFGVACICDIY 407
>TC221367 weakly similar to UP|Q84VY9 (Q84VY9) At1g30130, partial (52%)
Length = 1133
Score = 29.6 bits (65), Expect = 4.6
Identities = 13/51 (25%), Positives = 25/51 (48%)
Frame = -1
Query: 302 WCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYI 352
+CK L R +PT + ++ + +G + +W E F P C+C++
Sbjct: 485 YCKILSRIICFDIPTSKHIHKRTDMQSLGH*IRVWVEKKRYSFTPWCICHL 333
>TC233081
Length = 642
Score = 28.9 bits (63), Expect = 7.8
Identities = 16/54 (29%), Positives = 25/54 (45%)
Frame = +1
Query: 161 LKAVNMTQSMEVDREILETQDKVAEKTEILVPYNILPLDPDSANQAIMRFPEIQ 214
LKA + E + + +K K L PY+I DPD+ +Q R P ++
Sbjct: 49 LKAALARKEGESEHSFSSSSEKYRTKASELSPYHINQRDPDTVDQLGCRQPMVE 210
>TC206835 similar to GB|AAQ56832.1|34098899|BT010389 At2g34040 {Arabidopsis
thaliana;} , partial (52%)
Length = 1184
Score = 28.9 bits (63), Expect = 7.8
Identities = 16/53 (30%), Positives = 25/53 (46%), Gaps = 9/53 (16%)
Frame = +2
Query: 337 GEAANLRFMPECLCYIYHHMAFEL---------YGMLAGNVSPMTGENIKPAY 380
GE N ++ ECL Y +HH+A ++ Y ++ G S GE+ Y
Sbjct: 221 GEEMNFTYV-ECLLYTFHHLAHKVPNATNSLCGYKIVTGQPSDRLGEDFSEHY 376
>BU546627 similar to GP|11994328|dbj receptor protein-like {Arabidopsis
thaliana}, partial (3%)
Length = 603
Score = 28.9 bits (63), Expect = 7.8
Identities = 11/13 (84%), Positives = 13/13 (99%)
Frame = -3
Query: 486 SFDRMWSFFILSL 498
SFDR+WSFF+LSL
Sbjct: 352 SFDRIWSFFLLSL 314
>TC209529
Length = 777
Score = 28.9 bits (63), Expect = 7.8
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = -2
Query: 414 SQWRNYDDLNEYFWSADCFRLGWPMRADADFFCLPVEHLHFDKLK 458
++W NY D+ + FW LG+ M+ D+ +P+ H D++K
Sbjct: 155 TKWSNY*DIKKLFW------LGY-MQLDSTISLVPLSFKHSDEIK 42
>TC208937 similar to GB|AAM70562.1|21700877|AY124853 At1g74910/F9E10_24
{Arabidopsis thaliana;} , partial (41%)
Length = 714
Score = 28.9 bits (63), Expect = 7.8
Identities = 14/42 (33%), Positives = 19/42 (44%), Gaps = 3/42 (7%)
Frame = +2
Query: 409 GRSKHSQWRNY-DDLNEYF--WSADCFRLGWPMRADADFFCL 447
GR H W + D ++ F +S F GWP +FCL
Sbjct: 209 GRCDHGGWTHKRDQISTSFLQYSQTAFPFGWPANGSPSYFCL 334
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,436,683
Number of Sequences: 63676
Number of extensions: 441872
Number of successful extensions: 2272
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 2248
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2267
length of query: 676
length of database: 12,639,632
effective HSP length: 104
effective length of query: 572
effective length of database: 6,017,328
effective search space: 3441911616
effective search space used: 3441911616
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 62 (28.5 bits)
Medicago: description of AC137603.8