BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137546.8 + phase: 0 /pseudo
(374 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213164 125 2e-29
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 107 9e-24
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 106 2e-23
BI321779 99 2e-21
TC234193 57 2e-08
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 53 2e-07
CO982349 50 2e-06
BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase ... 47 1e-05
TC206673 45 5e-05
TC218847 45 5e-05
CA851997 35 9e-04
BG405621 35 0.055
BI784748 33 0.16
CA851030 33 0.27
TC207274 similar to UP|Q940C8 (Q940C8) AT3g10420/F13M14_30, part... 33 0.27
TC227836 weakly similar to UP|Q8LPZ2 (Q8LPZ2) Ser-Thr protein ki... 30 1.8
TC233341 similar to UP|Q8PWH4 (Q8PWH4) Conserved protein, partia... 27 2.2
TC212583 29 3.0
TC227835 weakly similar to UP|DYR3_HUMAN (O43781) Dual-specifici... 29 3.0
AW164615 29 3.9
>TC213164
Length = 446
Score = 125 bits (315), Expect = 2e-29
Identities = 59/135 (43%), Positives = 92/135 (67%)
Frame = +3
Query: 162 LIKPFSVDEVKEAICDYDSFKSSGPDGVNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKG 221
L+ F EV+ + D + KS GPDG+NF FIKEFW +LK D+++F+ EF+ NG K
Sbjct: 18 LVARFEKKEVRATMWDRGNAKSPGPDGLNFKFIKEFWDILKTDLLRFLDEFYANGVFLKR 197
Query: 222 INTTFIVLISKVDNPQRLNDFRPISLVGSMNKIIAKLLANRLRLVIGSVVSETQSAFVKK 281
N F+ LI KV +P LN+++PISL+G + KI+AKLL+ R + V+ +++ E Q+ F++
Sbjct: 198 SNAFFLALIPKVHDP*SLNEYKPISLIGCIYKIVAKLLSGRHKKVLPTIIDERQTVFMEG 377
Query: 282 RQILDGILIANEVVD 296
R L ++IANE+++
Sbjct: 378 RHTLHNVVIANEIME 422
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 107 bits (267), Expect = 9e-24
Identities = 53/90 (58%), Positives = 64/90 (70%)
Frame = +1
Query: 285 LDGILIANEVVDEAFKLKKDLMLFKVDFEKTYDSVDWGYLGEVLKCMSFPVLWRKWIREC 344
L LIANEV+DEA + K ++FKVD+EK YDSV W +L ++ M F W KWI EC
Sbjct: 1 LHSALIANEVIDEAKRSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWIEEC 180
Query: 345 VGTATASVLVNGSPTDEFPLKRGLRQGDPL 374
V +A+ SVLVNGSPT EF +RGLRQGDPL
Sbjct: 181 VKSASISVLVNGSPTAEFLPQRGLRQGDPL 270
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 106 bits (264), Expect = 2e-23
Identities = 51/88 (57%), Positives = 64/88 (71%)
Frame = -2
Query: 171 VKEAICDYDSFKSSGPDGVNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLI 230
+K A+ S KS GPDG+NF FIK FW LK DI++F++EFH NG KG N +FI LI
Sbjct: 375 IKSAVWQCGSVKSPGPDGLNFKFIKHFWERLKPDIIRFLNEFHANGIFPKGGNASFIALI 196
Query: 231 SKVDNPQRLNDFRPISLVGSMNKIIAKL 258
KV +PQ LNDFRPISL+G + KI+AK+
Sbjct: 195 PKVKHPQALNDFRPISLIGCVYKIVAKI 112
>BI321779
Length = 421
Score = 99.4 bits (246), Expect = 2e-21
Identities = 49/115 (42%), Positives = 74/115 (63%)
Frame = +2
Query: 143 RPSVSNLQFQALSVTEGEGLIKPFSVDEVKEAICDYDSFKSSGPDGVNFGFIKEFWIVLK 202
RP ++ ++F + S + LI+ F V+E+K+ I D +S KS GPDG + IK FW V+K
Sbjct: 26 RPKLNGVEFLSFSNEDNAMLIQDFEVEEIKKVI*DCESSKSPGPDGYSLLLIKIFWEVIK 205
Query: 203 DDIMQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPISLVGSMNKIIAK 257
++++ F EFH+ L +G N +FI LI+K DNP + + PISLVG + KI+ K
Sbjct: 206 EEVINFFKEFHKFDFLPRGTNRSFIALIAKCDNPLDIGQYCPISLVGCLYKIV*K 370
>TC234193
Length = 697
Score = 56.6 bits (135), Expect = 2e-08
Identities = 28/67 (41%), Positives = 44/67 (64%)
Frame = -3
Query: 187 DGVNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLISKVDNPQRLNDFRPIS 246
+G NF FIK+ V+K+D+++ + EFH +G L++G N +F+ P L DFRP++
Sbjct: 194 NGYNFNFIKKC*EVMKEDVVRAIQEFHSHGCLSRGTNASFL-----THPP*GLGDFRPVT 30
Query: 247 LVGSMNK 253
LVGS+ K
Sbjct: 29 LVGSLCK 9
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 53.1 bits (126), Expect = 2e-07
Identities = 26/69 (37%), Positives = 39/69 (55%)
Frame = -3
Query: 306 MLFKVDFEKTYDSVDWGYLGEVLKCMSFPVLWRKWIRECVGTATASVLVNGSPTDEFPLK 365
+ K+D K +D++D +L VL + + WIR + +A S+ VNG P F +
Sbjct: 765 LALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCNWIRVILLSAKLSISVNGEPVGFFSCQ 586
Query: 366 RGLRQGDPL 374
RG+RQGDPL
Sbjct: 585 RGVRQGDPL 559
>CO982349
Length = 795
Score = 50.1 bits (118), Expect = 2e-06
Identities = 22/35 (62%), Positives = 28/35 (79%)
Frame = +3
Query: 340 WIRECVGTATASVLVNGSPTDEFPLKRGLRQGDPL 374
WIR C+ +A+ S+LVN SP +EF +RGLRQGDPL
Sbjct: 69 WIRGCLHSASVSILVNESPINEFSPQRGLRQGDPL 173
>BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase (EC
2.7.7.49) (clone AmLi2) - garden snapdragon (fragment),
partial (22%)
Length = 111
Score = 47.0 bits (110), Expect = 1e-05
Identities = 20/32 (62%), Positives = 26/32 (80%)
Frame = +1
Query: 342 RECVGTATASVLVNGSPTDEFPLKRGLRQGDP 373
+EC+ +A+ S+LVNGSPT+EF RGLRQG P
Sbjct: 1 KECLSSASISILVNGSPTEEFKPXRGLRQGGP 96
>TC206673
Length = 742
Score = 45.1 bits (105), Expect = 5e-05
Identities = 17/41 (41%), Positives = 31/41 (75%)
Frame = +3
Query: 74 SICWQQSRNHWLREGDMNSKFFHSLMSSRRRFNTICSVLVE 114
S+ Q+SR+ WL+EGD NS +FH++++ RR +N + +L++
Sbjct: 150 SLLRQKSRDKWLKEGDNNSAYFHTVINFRRHYNGLQGILIQ 272
>TC218847
Length = 521
Score = 45.1 bits (105), Expect = 5e-05
Identities = 26/88 (29%), Positives = 45/88 (50%), Gaps = 1/88 (1%)
Frame = -3
Query: 74 SICWQQSRNHWLREGDMNSKFFHSLMSSRRRFNTICSVLVEDVRIEGVEPVR-NAIFTHF 132
S+ Q++R W+RE D NS +FH L++ R N + V++ +E EP R A+ F
Sbjct: 258 SLLRQKARTKWIRERDCNSSYFHKLINYIHRTNAVNGVMIGGSWVE--EPNRVKAVRNFF 85
Query: 133 AQHFMSHNVVRPSVSNLQFQALSVTEGE 160
F + RP ++ F+++ + E
Sbjct: 84 QIRFSESDYDRP*INGANFRSIGQQQNE 1
>CA851997
Length = 636
Score = 35.0 bits (79), Expect(2) = 9e-04
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +1
Query: 186 PDGVNFGFIKEFWIVLKDDIMQFVSEFHRNGKLAKGINTTFIVLISKV 233
PDG N GF FW + +D+ Q + G +N T I +I ++
Sbjct: 316 PDGFNLGFYHRFWGMCGEDVFQACCMWLAEGAFPSSVNDTTIAIILEI 459
Score = 25.0 bits (53), Expect(2) = 9e-04
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = +2
Query: 232 KVDNPQRLNDFRPISLV 248
K DNP+ + D RPIS V
Sbjct: 455 KFDNPRGMKDLRPISYV 505
>BG405621
Length = 402
Score = 35.0 bits (79), Expect = 0.055
Identities = 15/22 (68%), Positives = 18/22 (81%), Gaps = 1/22 (4%)
Frame = +2
Query: 77 WQQ-SRNHWLREGDMNSKFFHS 97
WQQ S+ WL+ GD+NSKFFHS
Sbjct: 335 WQQRSKIFWLKYGDLNSKFFHS 400
>BI784748
Length = 259
Score = 33.5 bits (75), Expect = 0.16
Identities = 15/29 (51%), Positives = 23/29 (78%)
Frame = +2
Query: 275 QSAFVKKRQILDGILIANEVVDEAFKLKK 303
QSAF++ R +L ++IANEV+DEA + +K
Sbjct: 14 QSAFIQGRHMLHSMMIANEVIDEAKRGQK 100
>CA851030
Length = 358
Score = 32.7 bits (73), Expect = 0.27
Identities = 15/36 (41%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Frame = +1
Query: 77 WQQ-SRNHWLREGDMNSKFFHSLMSSRRRFNTICSV 111
WQQ SR W +E D N++FFH R+ N+I +
Sbjct: 184 WQQRSRAIWFKERDKNTEFFHRKTLQRKASNSITKI 291
>TC207274 similar to UP|Q940C8 (Q940C8) AT3g10420/F13M14_30, partial (31%)
Length = 1001
Score = 32.7 bits (73), Expect = 0.27
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 312 FEKTYDSVDWGYLGEVLKCMSFPVLWRKWIREC 344
++KT+ SV W L +S P+ W KW R+C
Sbjct: 284 YDKTHGSVVWQSSITYLYLLSSPIPWHKWSRQC 382
>TC227836 weakly similar to UP|Q8LPZ2 (Q8LPZ2) Ser-Thr protein kinase-like,
partial (5%)
Length = 677
Score = 30.0 bits (66), Expect = 1.8
Identities = 20/46 (43%), Positives = 26/46 (56%)
Frame = +2
Query: 39 VWRCWMGKGKWRTCQTLNVMSYIVFRRRFIP*LNTSICWQQSRNHW 84
+WRCW KGK RT + M +I RR I N S +Q+S +HW
Sbjct: 65 IWRCW-*KGKKRTSTSPKNMIFIT*TRRLI---NWST*FQKS-HHW 187
>TC233341 similar to UP|Q8PWH4 (Q8PWH4) Conserved protein, partial (7%)
Length = 714
Score = 26.6 bits (57), Expect(2) = 2.2
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = -1
Query: 50 RTCQTLNVMSYIVFRRRFIP*LNTSICWQQSR-NHWLREGDMN 91
R C L S + F R +P L++ Q+ R +HW+ G N
Sbjct: 138 RVCLRLGCFSALGFSRHLLPLLDSDFPQQRQRHHHWVLRGKGN 10
Score = 21.6 bits (44), Expect(2) = 2.2
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = -2
Query: 34 SNLKTVWRCWMGKGKWR 50
SN+ TVWR G+G+ R
Sbjct: 299 SNIYTVWREVKGRGQER 249
>TC212583
Length = 530
Score = 29.3 bits (64), Expect = 3.0
Identities = 11/17 (64%), Positives = 13/17 (75%)
Frame = +2
Query: 84 WLREGDMNSKFFHSLMS 100
W EGD NSKFFHS ++
Sbjct: 5 WFLEGDHNSKFFHSYVN 55
>TC227835 weakly similar to UP|DYR3_HUMAN (O43781) Dual-specificity
tyrosine-phosphorylation regulated kinase 3 , partial
(14%)
Length = 1332
Score = 29.3 bits (64), Expect = 3.0
Identities = 20/46 (43%), Positives = 27/46 (58%)
Frame = +2
Query: 39 VWRCWMGKGKWRTCQTLNVMSYIVFRRRFIP*LNTSICWQQSRNHW 84
+WRCW KGK RT + M +I+ RR I N S +Q+S +HW
Sbjct: 677 IWRCW*-KGKKRTSTSPKNMIFIM*MRRRI---NWST*FQKS-HHW 799
>AW164615
Length = 442
Score = 28.9 bits (63), Expect = 3.9
Identities = 13/41 (31%), Positives = 19/41 (45%)
Frame = -2
Query: 44 MGKGKWRTCQTLNVMSYIVFRRRFIP*LNTSICWQQSRNHW 84
M K C + N+M+ + P L TS+CWQ+ W
Sbjct: 273 MSLHKRAQCFSRNLMARMKPEANMAPTLRTSLCWQRPHRSW 151
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.333 0.145 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,707,095
Number of Sequences: 63676
Number of extensions: 242997
Number of successful extensions: 2128
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 2110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2126
length of query: 374
length of database: 12,639,632
effective HSP length: 99
effective length of query: 275
effective length of database: 6,335,708
effective search space: 1742319700
effective search space used: 1742319700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC137546.8