BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136954.8 + phase: 0
(482 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220903 similar to UP|Q9FHH4 (Q9FHH4) Diacylglycerol kinase-lik... 327 6e-90
TC233509 similar to UP|KDG1_ARATH (Q39017) Diacylglycerol kinase... 80 2e-15
TC208947 79 6e-15
TC234216 similar to UP|Q9FVC9 (Q9FVC9) Diacylglycerol kinase var... 76 3e-14
BG405312 65 2e-12
AW185598 68 1e-11
BG550911 similar to GP|10798890|gb| calmodulin-binding diacylgly... 62 4e-10
TC223693 similar to UP|Q9FVD1 (Q9FVD1) Diacylglycerol kinase, pa... 58 1e-08
TC213323 48 3e-08
TC213349 55 5e-08
TC224515 similar to UP|Q9FVC9 (Q9FVC9) Diacylglycerol kinase var... 55 9e-08
TC232715 similar to UP|KDG1_ARATH (Q39017) Diacylglycerol kinase... 54 2e-07
AW350705 38 0.011
TC235142 similar to UP|O22681 (O22681) Arabidopsis thaliana gl1 ... 30 2.4
TC208044 29 5.3
BE021416 28 7.0
TC216712 28 7.0
TC216393 similar to UP|Q9FHL3 (Q9FHL3) Arabidopsis thaliana geno... 28 7.0
TC234977 28 9.1
>TC220903 similar to UP|Q9FHH4 (Q9FHH4) Diacylglycerol kinase-like protein,
partial (34%)
Length = 829
Score = 327 bits (839), Expect = 6e-90
Identities = 151/170 (88%), Positives = 164/170 (95%)
Frame = +2
Query: 312 KIIYSGYSCTQGWFFTPCTSDPGLRGLRNILRMHIKRVSSSEWEQVAIPKSVRAIVALNL 371
KIIYSGYSCTQGWFFTPC SDPGLRGL+NILRMHIKR +SSEWEQ+AIP SVRAIVALNL
Sbjct: 2 KIIYSGYSCTQGWFFTPCVSDPGLRGLKNILRMHIKRANSSEWEQIAIPTSVRAIVALNL 181
Query: 372 HSYGSGRNPWGKPKPEYLEKKGFVEADVADGRLEIFGLKQGWHASFVMVDLITAKHIAQA 431
HSYGSGRNPWGKPKPEYLEK+GFVEADVADG LE+FGLKQGWHASFVMV+LI+AKH+AQA
Sbjct: 182 HSYGSGRNPWGKPKPEYLEKRGFVEADVADGLLEVFGLKQGWHASFVMVELISAKHLAQA 361
Query: 432 AAIRLELRGGGWKNAYLQMDGEPWKQPLSKDFSTFVEIKREPFQSLVVDG 481
+AIRLE+RGG WKNAY+QMDGEPWKQPLSKDFST+VEIKREPFQSLV+ G
Sbjct: 362 SAIRLEVRGGQWKNAYMQMDGEPWKQPLSKDFSTYVEIKREPFQSLVISG 511
>TC233509 similar to UP|KDG1_ARATH (Q39017) Diacylglycerol kinase 1
(Diglyceride kinase 1) (DGK 1) (DAG kinase 1) , partial
(19%)
Length = 421
Score = 80.5 bits (197), Expect = 2e-15
Identities = 52/149 (34%), Positives = 71/149 (46%), Gaps = 1/149 (0%)
Frame = +1
Query: 153 RVMVAGGDGTVGWVLGCLTELRQLGREPVPPVGIVPLGTGNDLSRSFNWGGSF-PFAWKS 211
RV+V GGDGTVGWVL + + + + PPV I+P GTGNDL+R +WGG P +
Sbjct: 67 RVLVCGGDGTVGWVLNAIDKQKFVSP---PPVAILPAGTGNDLARVLSWGGGLGPVERQG 237
Query: 212 AIKRTLQKASVGSVHRLDSWRLSISMPESTTVKPPYCLKQAEEFTLDQGIEIEGELPDKV 271
+ L +V LD W+++IS P+ + LP K
Sbjct: 238 GLTTFLHHIEHAAVTVLDRWKVTISNPQGK----------------------QQLLPTKF 351
Query: 272 KSYEGVYYNYFSIGMDAQVAYGFHRLRDE 300
+ NY IG DA+VA H LR+E
Sbjct: 352 MN------NYLGIGCDAKVALDIHNLREE 420
>TC208947
Length = 598
Score = 78.6 bits (192), Expect = 6e-15
Identities = 43/104 (41%), Positives = 62/104 (59%)
Frame = +2
Query: 78 DSAAPTSPMVVFINARSGGRHGPALKERLQQLMSEEQVFDLADVKPHEFVLYGLACLEML 137
D P P++VF+N+RSGG+ G L + + L++E QVFDL + P + + A LE L
Sbjct: 281 DIEGPKCPVLVFVNSRSGGQLGGELLKTYRALLNENQVFDLGEEAPDKVLSRIYANLENL 460
Query: 138 AGLGDSCAKETREKLRVMVAGGDGTVGWVLGCLTELRQLGREPV 181
GD A + EKLR++ AGGDGT GW+LG +L+ P+
Sbjct: 461 RLQGDHIAIQIMEKLRLIGAGGDGTAGWLLGVGCDLKLSHPPPI 592
>TC234216 similar to UP|Q9FVC9 (Q9FVC9) Diacylglycerol kinase variant B,
partial (28%)
Length = 445
Score = 76.3 bits (186), Expect = 3e-14
Identities = 42/116 (36%), Positives = 62/116 (53%), Gaps = 2/116 (1%)
Frame = +3
Query: 132 ACLEMLAGLGDSCAKETREKLRVMVAGGDGTVGWVLGCLTELRQLGREPVPPVGIVPLGT 191
A LE L GD+ A E + +LR++VAGGDGT W+LG +++L+ PP+ VPLGT
Sbjct: 30 ATLETLKHNGDNFAAEIQNRLRIIVAGGDGTASWLLGVVSDLK---LPQPPPIATVPLGT 200
Query: 192 GNDLSRSFNWGGSFPFAWKSAIKRTLQKASVGSVHRLDSWR--LSISMPESTTVKP 245
GN+L +F WG P ++ L ++DSW + I P+ + P
Sbjct: 201 GNNLPFAFGWGKKNPTTDLQSVVSFLNHVKGAREMKIDSWHIIMRIKAPKEGSCDP 368
>BG405312
Length = 415
Score = 64.7 bits (156), Expect(2) = 2e-12
Identities = 33/101 (32%), Positives = 50/101 (48%), Gaps = 1/101 (0%)
Frame = +1
Query: 338 LRNILRM-HIKRVSSSEWEQVAIPKSVRAIVALNLHSYGSGRNPWGKPKPEYLEKKGFVE 396
L NI ++ +K + WE + IP+S+++IV LNL S+ G NPWG P +
Sbjct: 106 LLNIAQLAKVKIMKKGHWEDLHIPRSIKSIVCLNLPSFSGGLNPWGTPNRRKSIYRDLTL 285
Query: 397 ADVADGRLEIFGLKQGWHASFVMVDLITAKHIAQAAAIRLE 437
V DG E+ G + WH ++ +AQ + IR E
Sbjct: 286 PYVDDGLFEVVGFRDAWHGLVLLAPKGHGTRLAQTSRIRFE 408
Score = 25.4 bits (54), Expect(2) = 2e-12
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = +3
Query: 311 NKIIYSGYSCTQGWFF 326
N+ Y CTQGWFF
Sbjct: 36 NQSTYLKLGCTQGWFF 83
>AW185598
Length = 437
Score = 67.8 bits (164), Expect = 1e-11
Identities = 32/41 (78%), Positives = 37/41 (90%)
Frame = +3
Query: 7 STTEDSKKIQVRSSLVESIRGCGLSGMRIDKEDLKKQLTLP 47
STT D+ KI VRSSLVES RGCG+SG+RIDKE+LKKQLT+P
Sbjct: 315 STTGDTNKIAVRSSLVESFRGCGISGIRIDKEELKKQLTMP 437
>BG550911 similar to GP|10798890|gb| calmodulin-binding diacylglycerol kinase
{Lycopersicon esculentum}, partial (14%)
Length = 212
Score = 62.4 bits (150), Expect = 4e-10
Identities = 32/70 (45%), Positives = 45/70 (63%)
Frame = +2
Query: 132 ACLEMLAGLGDSCAKETREKLRVMVAGGDGTVGWVLGCLTELRQLGREPVPPVGIVPLGT 191
A LE L G A + +E+L+++VAGGDGT GW+LG + +L+ PP+ VPLGT
Sbjct: 11 ANLEGLNVRGYQFADKIKERLKLIVAGGDGTAGWLLGVVCDLK---LSHPPPIATVPLGT 181
Query: 192 GNDLSRSFNW 201
GN+L +F W
Sbjct: 182 GNNLPFAFGW 211
>TC223693 similar to UP|Q9FVD1 (Q9FVD1) Diacylglycerol kinase, partial (29%)
Length = 657
Score = 57.8 bits (138), Expect = 1e-08
Identities = 30/72 (41%), Positives = 40/72 (54%), Gaps = 1/72 (1%)
Frame = +3
Query: 399 VADGRLEIFGLKQGWHASFVMVDLITAKHIAQAAAIRLELRGGGWKNAYLQMDGEPWKQP 458
V DG LEI G + WH ++ +AQA I+ E R G + ++++DGEPWKQP
Sbjct: 57 VDDGLLEIVGFRNAWHGLVLLAPKGHGTRLAQAHRIQFEFRKGAADHTFMRIDGEPWKQP 236
Query: 459 LSKDFST-FVEI 469
L D T VEI
Sbjct: 237 LPVDDDTVMVEI 272
>TC213323
Length = 541
Score = 47.8 bits (112), Expect(2) = 3e-08
Identities = 31/116 (26%), Positives = 50/116 (42%), Gaps = 13/116 (11%)
Frame = +3
Query: 182 PPVGIVPLGTGNDLSRSFNWGGSFPFAWKSAIKRTLQKASVGSVHRLDSWRLSISM--PE 239
PP+ VPLGTGN+L +F WG P + A+K L + ++D+W + + M P+
Sbjct: 78 PPIATVPLGTGNNLPFAFGWGKKNPGTDEQAVKSVLDQVMKAKEMKIDNWHILMRMRAPK 257
Query: 240 STTVKPPYCLKQAEEFTLDQGIEIEGEL-----------PDKVKSYEGVYYNYFSI 284
P L+ I EL + ++ G ++NYFS+
Sbjct: 258 HGPCDPIPPLELTHSLHAFHHISEADELNVGLLLFLIAPQEGCHTFRGGFWNYFSM 425
Score = 28.1 bits (61), Expect(2) = 3e-08
Identities = 10/19 (52%), Positives = 11/19 (57%)
Frame = +1
Query: 315 YSGYSCTQGWFFTPCTSDP 333
Y+ CTQGWFF P P
Sbjct: 433 YAKLGCTQGWFFAPLFLPP 489
>TC213349
Length = 421
Score = 55.5 bits (132), Expect = 5e-08
Identities = 28/72 (38%), Positives = 39/72 (53%), Gaps = 1/72 (1%)
Frame = +3
Query: 399 VADGRLEIFGLKQGWHASFVMVDLITAKHIAQAAAIRLELRGGGWKNAYLQMDGEPWKQP 458
V DG +E+ G + WH ++ +AQA IR E G + ++++DGEPWKQP
Sbjct: 21 VDDGLIEVVGFRDAWHGLVLLAPNGHGTRLAQAHRIRFEFHKGAAEYTFMRIDGEPWKQP 200
Query: 459 LSKDFST-FVEI 469
L D T VEI
Sbjct: 201 LPVDDDTVLVEI 236
>TC224515 similar to UP|Q9FVC9 (Q9FVC9) Diacylglycerol kinase variant B,
partial (20%)
Length = 760
Score = 54.7 bits (130), Expect = 9e-08
Identities = 27/88 (30%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = -1
Query: 328 PCTSDPGL-RGLRNILRMHIKRVSSSEWEQVAIPKSVRAIVALNLHSYGSGRNPWGKPKP 386
PC + G + N R+ + + + + IP+S+++IV LNL S+ G NPWG P
Sbjct: 283 PCETQNGFCLSVPNEFRVPLVQAKQDHAKHLHIPRSIKSIVCLNLPSFSGGLNPWGTPNR 104
Query: 387 EYLEKKGFVEADVADGRLEIFGLKQGWH 414
+ + V DG E+ G + WH
Sbjct: 103 KKSIYRDLTLPYVDDGLFEVVGFRDAWH 20
Score = 42.4 bits (98), Expect = 5e-04
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = +1
Query: 413 WHASFVMVDLITAKHIAQAAAIRLELRGGGWKNAYLQMDGEPWKQPLSKD 462
WH ++ +AQ + IR E G ++++DGEPWK PL KD
Sbjct: 199 WHGLVLLAPKGHGTRLAQTSRIRFEFHKGAADCTFMRIDGEPWKPPLPKD 348
>TC232715 similar to UP|KDG1_ARATH (Q39017) Diacylglycerol kinase 1
(Diglyceride kinase 1) (DGK 1) (DAG kinase 1) , partial
(29%)
Length = 813
Score = 53.5 bits (127), Expect = 2e-07
Identities = 40/170 (23%), Positives = 69/170 (40%)
Frame = +1
Query: 288 AQVAYGFHRLRDEKPYLASGPIANKIIYSGYSCTQGWFFTPCTSDPGLRGLRNILRMHIK 347
A A H R+E P NK++Y+ D L +R+ +
Sbjct: 1 AXXALDIHNXREENPDKFYNQFMNKVLYAXXX-------XKSIMDRTFADLXWQIRVEVD 159
Query: 348 RVSSSEWEQVAIPKSVRAIVALNLHSYGSGRNPWGKPKPEYLEKKGFVEADVADGRLEIF 407
V ++ +P+ ++ N+ SY G + W Y F + + D LE+
Sbjct: 160 GV------EIEVPEDAEGVLVANIGSYMGGVDLWQNEDENY---DNFDQQSMHDKILEVV 312
Query: 408 GLKQGWHASFVMVDLITAKHIAQAAAIRLELRGGGWKNAYLQMDGEPWKQ 457
+ WH + V L A+ +AQ +I+++L + +Q+DGEPW Q
Sbjct: 313 SISGTWHLGKLQVGLSRARRLAQGQSIKIQL----FAMFPVQIDGEPWFQ 450
>AW350705
Length = 576
Score = 37.7 bits (86), Expect = 0.011
Identities = 24/67 (35%), Positives = 30/67 (43%), Gaps = 1/67 (1%)
Frame = -1
Query: 404 LEIFGLKQGWHASFVMVDLITAKHIAQAAAIRLELRGGGWKNAYLQMDGEPWKQPLSKDF 463
L +G K WH A IR E G + ++++DGEPWKQPL D
Sbjct: 495 LLFYGFKLSWH*---------------AHRIRFEFHKGAAEYTFMRIDGEPWKQPLPVDD 361
Query: 464 ST-FVEI 469
T VEI
Sbjct: 360 DTVLVEI 340
>TC235142 similar to UP|O22681 (O22681) Arabidopsis thaliana gl1 homolog,
partial (12%)
Length = 336
Score = 30.0 bits (66), Expect = 2.4
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = -3
Query: 270 KVKSYEGVYYNYFSIGMDAQVAYGFH 295
+V++Y+GVY YF G ++A+G H
Sbjct: 262 EVETYDGVYMRYFRKGNAVKMAFGMH 185
>TC208044
Length = 802
Score = 28.9 bits (63), Expect = 5.3
Identities = 20/67 (29%), Positives = 31/67 (45%), Gaps = 1/67 (1%)
Frame = -3
Query: 121 VKPHEFVLYGLACLEMLAGLGDSCAKETREKLRVMVAGGDGTVGW-VLGCLTELRQLGRE 179
VKPH+ + A +L +G +C T + L ++ GDG+ W V+ L Q
Sbjct: 452 VKPHDLIPIRTASRILLIVMGRACFGPTLDGLAKLLEAGDGS--WAVVDFKNHLDQAREG 279
Query: 180 PVPPVGI 186
PP G+
Sbjct: 278 LWPPAGV 258
>BE021416
Length = 411
Score = 28.5 bits (62), Expect = 7.0
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = +1
Query: 169 CLTELRQLGREPVPPVGIVPLGTGNDLSRSFNWGGSF 205
C +R+L E PP + LG+G ++R+ NWG SF
Sbjct: 31 CWVWIRRLS-ESEPPTRL--LGSGISVTRTLNWGNSF 132
>TC216712
Length = 599
Score = 28.5 bits (62), Expect = 7.0
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = -1
Query: 227 RLDSWRLSISMPESTTVKPPYCLKQAEEFTLD 258
++ + L I P T PP+ L AEEFTLD
Sbjct: 185 KMKTTNLLILQPAVTA*DPPFLLFDAEEFTLD 90
>TC216393 similar to UP|Q9FHL3 (Q9FHL3) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K19M13, partial (81%)
Length = 1946
Score = 28.5 bits (62), Expect = 7.0
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +1
Query: 154 VMVAGGDGTVGWVLGCLTELRQLGREPVP-PVGIVPLGTGNDL 195
++ GGDG + VL L R +E + P+GI+P G+ N L
Sbjct: 730 IICVGGDGIINEVLNGLLS-RDNQKEGISIPIGIIPAGSDNSL 855
>TC234977
Length = 808
Score = 28.1 bits (61), Expect = 9.1
Identities = 20/62 (32%), Positives = 31/62 (49%), Gaps = 5/62 (8%)
Frame = +2
Query: 334 GLRGLRNILRMHIKRVSSSEWEQ-----VAIPKSVRAIVALNLHSYGSGRNPWGKPKPEY 388
GL L+ ++R HI+R ++EW Q + + +RA A LHS S + G P+
Sbjct: 203 GLVKLQALVRGHIERKRTAEWLQRVQALLRVQAQIRAGRAQILHSPSSTSHLRGPATPDK 382
Query: 389 LE 390
E
Sbjct: 383 FE 388
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.136 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,041,392
Number of Sequences: 63676
Number of extensions: 291437
Number of successful extensions: 1380
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 1370
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1375
length of query: 482
length of database: 12,639,632
effective HSP length: 101
effective length of query: 381
effective length of database: 6,208,356
effective search space: 2365383636
effective search space used: 2365383636
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC136954.8