BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136141.8 - phase: 0
(339 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP595172 polyprotein [Glycine max] 122 3e-28
TC211627 118 3e-27
TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 87 8e-18
BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza... 83 2e-16
TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, parti... 78 7e-15
TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 72 5e-13
TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 64 7e-11
AW830191 53 2e-07
BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis ... 52 3e-07
BU549069 51 9e-07
BE807407 48 7e-06
TC232772 weakly similar to UP|Q9LQH2 (Q9LQH2) F15O4.13, partial ... 44 1e-04
TC228734 weakly similar to UP|Q99738 (Q99738) Pinin, partial (4%) 38 0.007
TC221808 homologue to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A ... 37 0.017
TC224715 homologue to UP|Q39882 (Q39882) Nodulin-26, partial (98%) 35 0.063
TC216484 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabin... 32 0.41
TC222530 weakly similar to UP|Q9LVF9 (Q9LVF9) Gb|AAB82628.1, par... 32 0.54
TC205446 weakly similar to UP|Q8LCN5 (Q8LCN5) Arabinogalactan-pr... 31 0.70
TC216930 similar to UP|Q9FWS8 (Q9FWS8) F1B16.7 protein, partial ... 31 0.92
TC205429 weakly similar to UP|O22600 (O22600) Glycine-rich prote... 31 0.92
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 122 bits (305), Expect = 3e-28
Identities = 65/148 (43%), Positives = 95/148 (63%), Gaps = 2/148 (1%)
Frame = +1
Query: 1 MTPFEALYGRPPPSIRNYVRGHTTVLKLDTTLAHIEHILHTIKENLERSKQRMGTLANQK 60
MTPF ALYGR PP++ ++ L + +L +K NL R++Q M A++K
Sbjct: 3859 MTPFRALYGREPPTLTRQACSIDDPAEVREQLTDRDALLAKLKINLTRAQQVMKRQADKK 4038
Query: 61 RKDCTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYELDLPPSSR 120
R D +F +G V ++LQ YRQH+ R + KL+ RY+GPF +L +IG VAY+L+LP ++R
Sbjct: 4039 RLDVSFQIGDEVLVKLQPYRQHSAVLRKNQKLSMRYFGPFKVLAKIGDVAYKLELPSAAR 4218
Query: 121 IHPVIHVSQLREYHG--QDPDGHFRPIP 146
IHPV HVSQL+ ++G QDP + P+P
Sbjct: 4219 IHPVFHVSQLKPFNGTAQDP---YLPLP 4293
>TC211627
Length = 1034
Score = 118 bits (296), Expect = 3e-27
Identities = 61/143 (42%), Positives = 91/143 (62%)
Frame = +3
Query: 7 LYGRPPPSIRNYVRGHTTVLKLDTTLAHIEHILHTIKENLERSKQRMGTLANQKRKDCTF 66
+YG+PPP++ Y G +TV +D L + I HT+ L++ + M +A+ R+D TF
Sbjct: 243 MYGKPPPALPLYSAGTSTVEAVDAILHSLATIHHTLTCRLQKYQDSMKRIADSHRRDLTF 422
Query: 67 VVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYELDLPPSSRIHPVIH 126
+G V++RL YRQ ++ + T KL+KR+YGP+ I R+G VAY L LPP+S+IHP+ H
Sbjct: 423 NIGDWVYVRL*PYRQTSI-QSTYTKLSKRFYGPYQIQARVGQVAYRLQLPPTSKIHPIFH 599
Query: 127 VSQLREYHGQDPDGHFRPIPPEV 149
VS L+ +HG PIPPE+
Sbjct: 600 VSLLKVHHG--------PIPPEL 644
>TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 730
Score = 87.4 bits (215), Expect = 8e-18
Identities = 40/95 (42%), Positives = 66/95 (69%)
Frame = +1
Query: 38 ILHTIKENLERSKQRMGTLANQKRKDCTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYY 97
+L T++ENL +S M N++R+D +VVG V+L++Q YR+ ++ +R + KL+ R+Y
Sbjct: 52 LLATLRENLLKS*DIM*ANTNKRRRDIEYVVGD*VFLKMQPYRRRSLAKRINEKLSPRFY 231
Query: 98 GPFLILRRIGPVAYELDLPPSSRIHPVIHVSQLRE 132
PF + ++G +AY+LDLP +IHPV HVS L++
Sbjct: 232 APFQVFNKVGTIAYKLDLPSHIKIHPVFHVSLLKK 336
>BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza sativa
(japonica cultivar-group)}, partial (3%)
Length = 424
Score = 83.2 bits (204), Expect = 2e-16
Identities = 40/90 (44%), Positives = 60/90 (66%)
Frame = +1
Query: 46 LERSKQRMGTLANQKRKDCTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRR 105
LER++ M AN R+ VG V+L+++ +RQ ++ R PKL R+YGP+L++R+
Sbjct: 25 LERAQSLMVKHANNHRRPHDINVGDWVYLKIRPHRQGSMPPRLHPKLTARFYGPYLVMRQ 204
Query: 106 IGPVAYELDLPPSSRIHPVIHVSQLREYHG 135
+G VA++L LP +RIHPV HVSQL+ G
Sbjct: 205 VGAVAFQLQLPSEARIHPVFHVSQLKRALG 294
>TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, partial (7%)
Length = 810
Score = 77.8 bits (190), Expect = 7e-15
Identities = 40/104 (38%), Positives = 67/104 (63%)
Frame = +3
Query: 53 MGTLANQKRKDCTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYE 112
M A++ R+ T VG V+L+LQ YR ++ ++ + KL+ R+YGP+ I ++IG VA+E
Sbjct: 3 MKAYADRSRRAVTLSVGDWVYLKLQPYRLKSLAKKRNEKLSPRFYGPYQIKKQIGLVAFE 182
Query: 113 LDLPPSSRIHPVIHVSQLREYHGQDPDGHFRPIPPEVANNLFNE 156
LDLPP+ +IHPV H S L++ + +P+P ++ +L +E
Sbjct: 183 LDLPPARKIHPVFHASLLKK--AVAATANPQPLPLMLSEDLSSE 308
>TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (5%)
Length = 761
Score = 71.6 bits (174), Expect = 5e-13
Identities = 35/79 (44%), Positives = 46/79 (57%)
Frame = +1
Query: 68 VGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYELDLPPSSRIHPVIHV 127
+G V ++L+ +RQ + T KL KRYYGPF + R+G V Y L L SRIHPV HV
Sbjct: 4 IGDWVLVKLRPHRQGSASETTYSKLTKRYYGPFEVQERLGKVVYRLKLTAHSRIHPVFHV 183
Query: 128 SQLREYHGQDPDGHFRPIP 146
S L+ + G H P+P
Sbjct: 184 SLLKAFVGDPETTHAGPLP 240
>TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 664
Score = 64.3 bits (155), Expect = 7e-11
Identities = 31/76 (40%), Positives = 48/76 (62%), Gaps = 2/76 (2%)
Frame = +2
Query: 74 LRLQLYRQHTVH--RRTSPKLAKRYYGPFLILRRIGPVAYELDLPPSSRIHPVIHVSQLR 131
L+L+ +RQ + + KL+KR++GPF ++ R+G VAY L LP +++HPV H S L+
Sbjct: 17 LKLRPHRQSSAKGPEPITGKLSKRFFGPFQVVERVGKVAYRLQLPVDAKLHPVFHCSLLK 196
Query: 132 EYHGQDPDGHFRPIPP 147
+ G PD P+PP
Sbjct: 197 PFQGNPPD-TAAPLPP 241
>AW830191
Length = 372
Score = 53.1 bits (126), Expect = 2e-07
Identities = 29/91 (31%), Positives = 52/91 (56%)
Frame = +3
Query: 1 MTPFEALYGRPPPSIRNYVRGHTTVLKLDTTLAHIEHILHTIKENLERSKQRMGTLANQK 60
+TPF+ LYG PP + +T +++ + +LH +K NL +++ +M A++
Sbjct: 102 LTPFKVLYGCDPPHLLKGAIISSTAEEVNVMTNDRDQMLHDLKGNLAKAQNQM-KYADRS 278
Query: 61 RKDCTFVVGQLVWLRLQLYRQHTVHRRTSPK 91
R+ VG V+L+LQ YRQ ++ R+T+ K
Sbjct: 279 RRSIPLNVGDWVYLKLQPYRQRSLARKTNEK 371
>BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis melo},
partial (9%)
Length = 410
Score = 52.4 bits (124), Expect = 3e-07
Identities = 37/101 (36%), Positives = 55/101 (53%), Gaps = 1/101 (0%)
Frame = -3
Query: 58 NQKRKDCTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYELDLPP 117
+++RKD F VG V+LR+ S KL + GPF IL+++ VAY++ LPP
Sbjct: 399 DKRRKDLEFEVGDHVFLRVTP*TG-VGRALKS*KLTPHFIGPFQILKKVDFVAYQIALPP 223
Query: 118 S-SRIHPVIHVSQLREYHGQDPDGHFRPIPPEVANNLFNES 157
S + +H V HVSQ+ +Y DP +V NL +E+
Sbjct: 222 SLTSLHNVFHVSQIHKYI-NDPSHMVNLDVVQVKENLAHET 103
>BU549069
Length = 615
Score = 50.8 bits (120), Expect = 9e-07
Identities = 32/70 (45%), Positives = 40/70 (56%), Gaps = 1/70 (1%)
Frame = -1
Query: 89 SPKLAKRYYGPFLILRRIGPVAYELDLPPS-SRIHPVIHVSQLREYHGQDPDGHFRPIPP 147
S KL + G F IL+R PVAY++ LPPS S +H V HVSQLR Y DP +
Sbjct: 573 SRKLTPHFIGHFQILKRAXPVAYQIALPPSLSNLHNVFHVSQLRMYI-HDPSHVVKLDDV 397
Query: 148 EVANNLFNES 157
+V NL E+
Sbjct: 396 QVKENLTYET 367
>BE807407
Length = 341
Score = 47.8 bits (112), Expect = 7e-06
Identities = 26/68 (38%), Positives = 37/68 (54%)
Frame = +2
Query: 64 CTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYELDLPPSSRIHP 123
C+F G +W L +R PKL RYYGP+L+L ++G VA++L LP H
Sbjct: 101 CSFFCGLPIWFDLP--------KRLHPKLTARYYGPYLVLIKVGVVAFQLQLPK----HA 244
Query: 124 VIHVSQLR 131
I +S +R
Sbjct: 245 *ITLSSMR 268
>TC232772 weakly similar to UP|Q9LQH2 (Q9LQH2) F15O4.13, partial (7%)
Length = 729
Score = 43.9 bits (102), Expect = 1e-04
Identities = 44/190 (23%), Positives = 76/190 (39%), Gaps = 4/190 (2%)
Frame = +1
Query: 3 PFEALYG-RPPPSIRNYVRGHTTVLKLDTTLAHIEHI--LHT-IKENLERSKQRMGTLAN 58
PFE +Y P + + + + K A +E+I LH K + + + N
Sbjct: 64 PFEVVYDFNPLTPLDSLPLSNISGFKHKDAHAKVEYIKRLHEQAKTQIAKKNESYVKQTN 243
Query: 59 QKRKDCTFVVGQLVWLRLQLYRQHTVHRRTSPKLAKRYYGPFLILRRIGPVAYELDLPPS 118
+ RK VW+ + R+ ++ KL R GPF +L RI AY++D+P
Sbjct: 244 KNRKKVVLEPSDWVWVHM---RKERFPKQRMSKLQPRGDGPFQVLERINYNAYKIDIPGE 414
Query: 119 SRIHPVIHVSQLREYHGQDPDGHFRPIPPEVANNLFNESPENNHQDTAEGRQEPETGKEG 178
+ +++ L + D R N F E + + TA+ Q T +
Sbjct: 415 YEVSSSFNIADLTPFVACDDSEDLRA-------NAFQEGRNDENPKTAQ-IQGTMTRSKT 570
Query: 179 QEKEKVISQM 188
++ E + QM
Sbjct: 571 KQSEDSLQQM 600
>TC228734 weakly similar to UP|Q99738 (Q99738) Pinin, partial (4%)
Length = 1156
Score = 37.7 bits (86), Expect = 0.007
Identities = 33/116 (28%), Positives = 47/116 (40%), Gaps = 2/116 (1%)
Frame = +1
Query: 201 SSTNVSSAVFKQGQMTLVPLDASQPQDACSTTQDKYPPLNPITTVGNQTLTDAPASPKIT 260
SS++ S G + P AS + ST PP P+ + P +P +
Sbjct: 289 SSSSSSW*SSSSGSSSAPPNPASSSKTPPSTPSTSPPPATPLPPLPQ------PPTPSPS 450
Query: 261 PSFIQSPPSDPTT-NHINGQHTTPCQLTESHRAPSHTEEHKPNLEVKV-SSGPDSS 314
P SPPS PTT + ++TP T + R+PS P SGP +S
Sbjct: 451 PCRSPSPPSTPTTASEFTTPNSTPTPPTAASRSPSPPPSPPPTRATATPPSGPPTS 618
>TC221808 homologue to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A ligase ,
partial (32%)
Length = 543
Score = 36.6 bits (83), Expect = 0.017
Identities = 29/87 (33%), Positives = 42/87 (47%), Gaps = 4/87 (4%)
Frame = +3
Query: 251 TDAPASPKITPSFIQ----SPPSDPTTNHINGQHTTPCQLTESHRAPSHTEEHKPNLEVK 306
T PA P TP+ I SPP+ +T + + T+ C + + R+ SH+ P L
Sbjct: 147 TATPAKPSPTPTSISPLAASPPA--STKSASARVTSSCSSSATARS-SHSPSWAP-LTAA 314
Query: 307 VSSGPDSSDRILDSHRSKRITTKPVWT 333
SS P + + S RSKR KP W+
Sbjct: 315 PSSPPPTPSTLRRSSRSKRWPRKPGWS 395
>TC224715 homologue to UP|Q39882 (Q39882) Nodulin-26, partial (98%)
Length = 1270
Score = 34.7 bits (78), Expect = 0.063
Identities = 27/97 (27%), Positives = 39/97 (39%), Gaps = 2/97 (2%)
Frame = +2
Query: 220 LDASQPQDACSTTQDKYPPL--NPITTVGNQTLTDAPASPKITPSFIQSPPSDPTTNHIN 277
L +SQP +CS Q P +P T + + P SP +PS S PS PT+
Sbjct: 575 LSSSQPSSSCSPAQAPASPTTSSPTTALPPLPASSPPPSPMHSPSSSPS-PSAPTSPAAT 751
Query: 278 GQHTTPCQLTESHRAPSHTEEHKPNLEVKVSSGPDSS 314
+P + + +PS + S P SS
Sbjct: 752 STPPSPSAPSSAATSPSSAASSTSSPSSSAPSSPPSS 862
>TC216484 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinogalactan
protein 1 precursor, partial (58%)
Length = 1213
Score = 32.0 bits (71), Expect = 0.41
Identities = 28/110 (25%), Positives = 37/110 (33%), Gaps = 10/110 (9%)
Frame = +1
Query: 202 STNVSSAVFKQGQMTLVPLDASQPQDA--CSTTQDKYPPLNPITTVGNQTLTDAPASPKI 259
ST S + + T P S P C T PP P T T + ++P+
Sbjct: 157 STTTSPSPTSPPKSTAKPPSPSAPSTTPPCRTFSRSTPPSTPSRTSSPSTSSSTTSAPRS 336
Query: 260 TPSFIQSPPSDPTTN-------HINGQHTTPCQLTE-SHRAPSHTEEHKP 301
+ +PPS P T+P E SH P T H P
Sbjct: 337 STRSPTAPPSPPQCTKPPAPPPDPPASSTSPTSTAEKSHSPPKTTTAHSP 486
Score = 29.3 bits (64), Expect = 2.7
Identities = 25/82 (30%), Positives = 34/82 (40%)
Frame = +1
Query: 249 TLTDAPASPKITPSFIQSPPSDPTTNHINGQHTTPCQLTESHRAPSHTEEHKPNLEVKVS 308
T T +PAS + TPS SPPS T + T+P + T +PS P S
Sbjct: 106 TRTTSPASSQSTPS---SPPSTTT----SPSPTSPPKSTAKPPSPSAPSTTPPCRTFSRS 264
Query: 309 SGPDSSDRILDSHRSKRITTKP 330
+ P + R S T+ P
Sbjct: 265 TPPSTPSRTSSPSTSSSTTSAP 330
>TC222530 weakly similar to UP|Q9LVF9 (Q9LVF9) Gb|AAB82628.1, partial (4%)
Length = 639
Score = 31.6 bits (70), Expect = 0.54
Identities = 23/78 (29%), Positives = 32/78 (40%)
Frame = -3
Query: 219 PLDASQPQDACSTTQDKYPPLNPITTVGNQTLTDAPASPKITPSFIQSPPSDPTTNHING 278
P AS + + ST Y PL+PIT+ + + P +P S ++ PT G
Sbjct: 268 PTTASSARASSSTLASSYSPLHPITSSPRNSSSSTP-NPTTRTSRVRCSTRPPTETPSRG 92
Query: 279 QHTTPCQLTESHRAPSHT 296
TPC A S T
Sbjct: 91 ---TPCSSATPSTACSAT 47
>TC205446 weakly similar to UP|Q8LCN5 (Q8LCN5) Arabinogalactan-protein,
partial (39%)
Length = 668
Score = 31.2 bits (69), Expect = 0.70
Identities = 18/43 (41%), Positives = 23/43 (52%), Gaps = 1/43 (2%)
Frame = +1
Query: 237 PPLNPITTVGNQTLTDAPAS-PKITPSFIQSPPSDPTTNHING 278
PPL+P +D+PA P +TPS I SPPS+ NG
Sbjct: 298 PPLSP---------SDSPAGVPSVTPSAISSPPSEAPAPSQNG 399
>TC216930 similar to UP|Q9FWS8 (Q9FWS8) F1B16.7 protein, partial (28%)
Length = 1869
Score = 30.8 bits (68), Expect = 0.92
Identities = 23/80 (28%), Positives = 36/80 (44%), Gaps = 3/80 (3%)
Frame = +1
Query: 219 PLDASQPQDACSTTQDKYPPLNPITTVGNQTLTDAPASPKITPSFIQSPPSDPTTNHING 278
P+D + + + + D N T V N + P PS +P +DP NH N
Sbjct: 697 PVDEALVANLMTPSSDLSMSSNFSTVVKNPAESSIPNLSFSIPSVFSTPTADPLPNH-NY 873
Query: 279 QH---TTPCQLTESHRAPSH 295
H +TP + +HR+P+H
Sbjct: 874 HHLPNSTPTRW--AHRSPAH 927
>TC205429 weakly similar to UP|O22600 (O22600) Glycine-rich protein, partial
(49%)
Length = 611
Score = 30.8 bits (68), Expect = 0.92
Identities = 22/74 (29%), Positives = 32/74 (42%), Gaps = 5/74 (6%)
Frame = +3
Query: 203 TNVSSAVFKQGQMTLVPLDASQP-QDACSTTQDK----YPPLNPITTVGNQTLTDAPASP 257
TN ++ + + + + L A+ P ACS+ Q K PP P+ T T P +P
Sbjct: 201 TN*TTIISRTKKKDVKKLGATHPCMHACSSFQGKGRTPLPPPTPVPTPPPPTPPPVPPNP 380
Query: 258 KITPSFIQSPPSDP 271
I P PP P
Sbjct: 381 PIPPVVPPRPPRPP 422
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.313 0.131 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,666,614
Number of Sequences: 63676
Number of extensions: 233893
Number of successful extensions: 1354
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 1314
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1349
length of query: 339
length of database: 12,639,632
effective HSP length: 98
effective length of query: 241
effective length of database: 6,399,384
effective search space: 1542251544
effective search space used: 1542251544
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC136141.8