BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135799.9 - phase: 0
(754 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229321 421 e-118
TC208281 143 3e-34
CF922641 105 1e-22
TC230428 weakly similar to UP|Q8RX89 (Q8RX89) AT5g23390/T32G24_2... 100 2e-21
AW568589 100 3e-21
AW509030 69 6e-12
CO985893 67 4e-11
BE823464 57 3e-08
BE823736 55 9e-08
TC224070 similar to UP|Q8RX89 (Q8RX89) AT5g23390/T32G24_2, parti... 55 2e-07
TC230427 52 7e-07
AW508721 38 0.019
TC217281 similar to UP|Q9M6R9 (Q9M6R9) Alpha-amylase , partial ... 31 1.8
TC227886 similar to UP|Q9M3Y5 (Q9M3Y5) 25.7 kDa protein, partial... 31 1.8
BU090294 30 4.0
TC218457 similar to PIR|T05166|T05166 quinone reductase homolog ... 30 4.0
CO981629 29 6.8
AW278165 similar to GP|14486705|gb phosphatidylinositol transfer... 29 6.8
TC214743 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane ph... 29 6.8
BI970752 similar to GP|10638981|emb protein phosphatase {Fagus s... 29 6.8
>TC229321
Length = 947
Score = 421 bits (1083), Expect = e-118
Identities = 214/274 (78%), Positives = 242/274 (88%)
Frame = +1
Query: 480 KGLVLSKNHVVADLSLTERAAKTSKQKSQVVEKTQATIDAATLKGIPSNIDLFKELIFPI 539
KGL+LSKN VVADLSL ERAAKTSK K +VEKTQATIDAATL+GIPSNIDLFKEL+FP
Sbjct: 1 KGLILSKNLVVADLSLIERAAKTSKHKYHIVEKTQATIDAATLQGIPSNIDLFKELMFPF 180
Query: 540 TLTAKNFEKLRHWEEPHLTVGFLGLAYTLIFRNLLSYIFPVMLMITAVGMLTIRSLKEQG 599
TL KNFEKLRHWEEPHLT+ FLGL YT+I+RNLLSY+FP+MLMI AVGMLTIR+LKEQG
Sbjct: 181 TLIVKNFEKLRHWEEPHLTIAFLGLTYTIIYRNLLSYMFPMMLMILAVGMLTIRALKEQG 360
Query: 600 RLGRFFGGVMIRDQPPSNTIQKIIAVKDAMRDVENMTQKVNVSLLKIRSILLSGNPQITT 659
RLGR FG V IRDQPPSNTIQKIIAVKDAMRDVEN Q+VNV LLKIRSILLSG+PQITT
Sbjct: 361 RLGRSFGEVTIRDQPPSNTIQKIIAVKDAMRDVENFMQQVNVFLLKIRSILLSGHPQITT 540
Query: 660 EVAVLMLTWATILFIVPFKYILSFLLFDMFTRELEFRREMVERLTKLLRERWHAVPAAPV 719
EVA+++++ ATIL I+PFKYI SFLLFDMFTRELEFRREMV++ LRERWH VPA PV
Sbjct: 541 EVALVLISSATILLIIPFKYIFSFLLFDMFTRELEFRREMVKKFRSFLRERWHTVPAVPV 720
Query: 720 AVLPFENEESKSEVSLKELENKSKPPGNQSKRKS 753
++LPFENE+ +SE+ LKE+E++SK GNQS KS
Sbjct: 721 SILPFENED-RSEIYLKEIEDQSKTQGNQSSVKS 819
>TC208281
Length = 807
Score = 143 bits (360), Expect = 3e-34
Identities = 82/221 (37%), Positives = 129/221 (58%)
Frame = +1
Query: 510 VEKTQATIDAATLKGIPSNIDLFKELIFPITLTAKNFEKLRHWEEPHLTVGFLGLAYTLI 569
V QAT+D + GI +N+ + KEL+FP+ AK+ + L +W++P ++ F +I
Sbjct: 10 VISAQATVDGVKVDGIDTNLAVMKELLFPLNELAKSLQSLAYWDDPRKSLVFCLFFSYII 189
Query: 570 FRNLLSYIFPVMLMITAVGMLTIRSLKEQGRLGRFFGGVMIRDQPPSNTIQKIIAVKDAM 629
+R L Y ++L++ AV M+ R + GR V + PP NT+++++AV++A+
Sbjct: 190 YRGWLGYAVALVLLLLAVFMIITRCFSQ----GRSVPEVKVIAPPPLNTMEQLLAVQNAV 357
Query: 630 RDVENMTQKVNVSLLKIRSILLSGNPQITTEVAVLMLTWATILFIVPFKYILSFLLFDMF 689
E + Q NV LLK R +LLS PQ T ++A +L+ A IL +P KYI+ L ++F
Sbjct: 358 SQAEQLIQDGNVILLKFRGLLLSIFPQATEKLAFGLLSTALILAFLPTKYIILLLFLEIF 537
Query: 690 TRELEFRREMVERLTKLLRERWHAVPAAPVAVLPFENEESK 730
TR R+ ERLT+ L+E W ++PAAPV L + EE K
Sbjct: 538 TRYSPPRKASTERLTRRLKEWWFSIPAAPV-TLERDKEEKK 657
>CF922641
Length = 582
Score = 105 bits (261), Expect = 1e-22
Identities = 52/67 (77%), Positives = 56/67 (82%)
Frame = +2
Query: 1 MSRTQNFLNEVTSPLAKTAQSRKPDPENDIGFQVMEDILMVEKTIDRKMPYGNLSLAAVI 60
MSRTQNF NEVT PLAK QSRKPD END GFQVMEDI M+E+T+DR+ P G LSLA VI
Sbjct: 314 MSRTQNFWNEVTFPLAKPGQSRKPDTENDCGFQVMEDIFMIEQTMDRRTPCGVLSLAVVI 493
Query: 61 CIEQFSR 67
CIEQFSR
Sbjct: 494 CIEQFSR 514
>TC230428 weakly similar to UP|Q8RX89 (Q8RX89) AT5g23390/T32G24_2, partial
(15%)
Length = 748
Score = 100 bits (250), Expect = 2e-21
Identities = 53/164 (32%), Positives = 96/164 (58%)
Frame = +3
Query: 574 LSYIFPVMLMITAVGMLTIRSLKEQGRLGRFFGGVMIRDQPPSNTIQKIIAVKDAMRDVE 633
+ Y P + M A+ ML R L++ GR ++ P N +++++ +++A+ E
Sbjct: 9 IQYFIPSIFMFMAILMLWRRHLRK----GRPLEAFIVTPPPNRNAVEQLLTLQEAITQFE 176
Query: 634 NMTQKVNVSLLKIRSILLSGNPQITTEVAVLMLTWATILFIVPFKYILSFLLFDMFTREL 693
++ Q N+ LLK+R++LL+ PQ T +VA+L++ A + VP KYIL + + +TRE+
Sbjct: 177 SLIQAANIILLKLRALLLAILPQATEKVALLLVFLAAVFAYVPPKYILLVVFVEFYTREM 356
Query: 694 EFRREMVERLTKLLRERWHAVPAAPVAVLPFENEESKSEVSLKE 737
+R+E +R + +RE W +PAAPV ++ +E K+E K+
Sbjct: 357 PYRKESSDRWIRRIREWWDRIPAAPVQLVKPVHESKKNESKKKK 488
>AW568589
Length = 407
Score = 100 bits (248), Expect = 3e-21
Identities = 49/70 (70%), Positives = 55/70 (78%)
Frame = +1
Query: 195 ISMSTWLAYINEFVKVRRENRSYQIPEFPQIDEEKILCIGSNSKQPVLKWENNMAWPGKL 254
I W Y + +VR + SYQIPEFPQ+ EE+ILCIGSNSK+PVLKWENNMA PGKL
Sbjct: 181 IQFQNWY*Y---YCRVREKQISYQIPEFPQLFEERILCIGSNSKRPVLKWENNMA*PGKL 351
Query: 255 TLTDKAIYFE 264
TLTDKAIYFE
Sbjct: 352 TLTDKAIYFE 381
>AW509030
Length = 443
Score = 69.3 bits (168), Expect = 6e-12
Identities = 46/129 (35%), Positives = 63/129 (48%), Gaps = 1/129 (0%)
Frame = +2
Query: 286 VEKAKVGPLGSSLFDSAVSISSGSESNWWVLEFIDLGGDMRRDVWHALISEVIALHKFTH 345
VE A GP G+ L+D A+ S S VLEF +L RRD W AL+ E++ LH+F
Sbjct: 2 VEPAATGPWGAQLYDKAIIYDSTDLSETVVLEFPELTSSTRRDHWLALVREIMFLHQFLS 181
Query: 346 EYGPDEYGPNVFEARKGKQRATSSAINGIARLQALQHLRKLLDD-PTKLVQFSYLQNAPN 404
+Y + + I GI RLQA + + ++ PTK + FS P
Sbjct: 182 KY--------QIKCPIQTWELHARTILGIVRLQAAREMLRISPPVPTKFLIFSLYDEIPK 337
Query: 405 GDIVLQSLA 413
GD VL+ LA
Sbjct: 338 GDYVLEELA 364
>CO985893
Length = 829
Score = 66.6 bits (161), Expect = 4e-11
Identities = 62/258 (24%), Positives = 110/258 (42%), Gaps = 7/258 (2%)
Frame = -1
Query: 324 DMRRDVWHALISEVIALHKFTHEYGPDEYGPNVFEARKGKQRATSSAINGIARLQALQHL 383
++RRD W + E++ HKF +Y E K + A+ GI R +A++
Sbjct: 820 NLRRDYWLDISLEILRAHKFIRKYYLKEVQ---------KSEVLARALLGIFRYRAVREA 668
Query: 384 RKLLDDPTK-LVQFSYLQNAPNGDIVLQSLAVNYWGSQLVTG------FTSTRHQPENRP 436
+ K L+ F+ + P GDI+LQ+++ + V+ T+ QP P
Sbjct: 667 FRFFSSHYKTLLTFNLAETLPRGDIILQTMSKSLTNLAAVSVKRDIPVTVDTKRQPAVSP 488
Query: 437 SNEIADSSNHVFDIDGSVYLRKWMKSPSWGSSTSTSFWKNTSTKGLVLSKNHVVADLSLT 496
+A YL + S T + + + V ++
Sbjct: 487 VAVMA-----------LFYL-------GFKSKKVTDICEEATFVSDIR-----VGEIHPL 377
Query: 497 ERAAKTSKQKSQVVEKTQATIDAATLKGIPSNIDLFKELIFPITLTAKNFEKLRHWEEPH 556
E A K S + E QAT+D ++GI +N+ + KEL+FP+ ++A + L W++ +
Sbjct: 376 EVAVKKSLLDTGKAEAAQATVDQVKVEGIDTNVAVMKELLFPVIVSANRLQLLASWKDFY 197
Query: 557 LTVGFLGLAYTLIFRNLL 574
+ FL L+ +I R L+
Sbjct: 196 KSAAFLLLSCYMIIR*LV 143
>BE823464
Length = 604
Score = 57.0 bits (136), Expect = 3e-08
Identities = 34/110 (30%), Positives = 58/110 (51%)
Frame = -3
Query: 617 NTIQKIIAVKDAMRDVENMTQKVNVSLLKIRSILLSGNPQITTEVAVLMLTWATILFIVP 676
+T++ I++ + + V +M Q N+++LKI SIL+S + V V M A +L ++P
Sbjct: 467 STMESIVSAQHGLYTVHDMMQIANIAMLKIWSILISKADKHANLVMVAMSGLAILLAVIP 288
Query: 677 FKYILSFLLFDMFTRELEFRREMVERLTKLLRERWHAVPAAPVAVLPFEN 726
FK+ L L+ FT L + + LRE W ++P P+ V+ N
Sbjct: 287 FKFFLMALILQSFTMTLG-KSSGSGTGNRRLREWWDSIPIVPIRVVDDPN 141
>BE823736
Length = 636
Score = 55.5 bits (132), Expect = 9e-08
Identities = 34/108 (31%), Positives = 57/108 (52%), Gaps = 2/108 (1%)
Frame = -2
Query: 617 NTIQKIIAVKDAMRDVENMTQKVNVSLLKIRSILLSGNPQITTEVAVLMLTWATILFIVP 676
+T++ I++ + + + +M Q N+++LKI SIL+S + V V M A +L ++P
Sbjct: 533 STMESIVSAQHGLYTIHDMMQIANIAMLKIWSILISKADKHANLVMVAMSGLAILLAVIP 354
Query: 677 FKYILSFLLFDMFTREL--EFRREMVERLTKLLRERWHAVPAAPVAVL 722
FK+ L L+ FT L F R LRE W ++P P+ V+
Sbjct: 353 FKFFLMALILQSFTMTLGKSFGSGTGNR---RLREWWDSIPIVPIRVV 219
>TC224070 similar to UP|Q8RX89 (Q8RX89) AT5g23390/T32G24_2, partial (16%)
Length = 521
Score = 54.7 bits (130), Expect = 2e-07
Identities = 41/118 (34%), Positives = 53/118 (44%), Gaps = 21/118 (17%)
Frame = +1
Query: 92 ARNLVEYCCFRFLSR--DNSDVHPSLQDPAFQRLIFITMLAWENPYTYVLSSNAE----- 144
AR+L+E+C ++ L + NSD L D F+RL F MLAWE P + L N
Sbjct: 10 ARHLLEFCSYKALHKLIHNSDF---LNDNDFRRLTFDMMLAWEAPSVHTLPDNPTSSSSK 180
Query: 145 --------------KASLQVAWVTLQSKRVTEEAFVRIAPAVSGVVDRPTVHNLFKVL 188
+S +A K V EAF RIAP + D TVHN+F L
Sbjct: 181 EETAGDEDDASLFYSSSTNMALQVDDKKTVGLEAFSRIAPVCIPIADVVTVHNIFHAL 354
>TC230427
Length = 457
Score = 52.4 bits (124), Expect = 7e-07
Identities = 24/68 (35%), Positives = 42/68 (61%)
Frame = +3
Query: 663 VLMLTWATILFIVPFKYILSFLLFDMFTRELEFRREMVERLTKLLRERWHAVPAAPVAVL 722
+L++ A + VP KYIL + + +TRE+ +R+E +R + +RE W +PAAPV ++
Sbjct: 3 LLLVFLAAVFAFVPPKYILLVVFVEFYTREMPYRKESSDRWIRRIREWWVRIPAAPVQLV 182
Query: 723 PFENEESK 730
++E K
Sbjct: 183 KPDHESKK 206
>AW508721
Length = 448
Score = 37.7 bits (86), Expect = 0.019
Identities = 17/48 (35%), Positives = 27/48 (55%)
Frame = +3
Query: 90 NDARNLVEYCCFRFLSRDNSDVHPSLQDPAFQRLIFITMLAWENPYTY 137
N R LVE+C + ++ ++ ++ D +F RL + MLAWE P Y
Sbjct: 84 NYCRKLVEFCSGKGVNEMCHNIEGNINDSSFSRLTYDMMLAWERPSYY 227
>TC217281 similar to UP|Q9M6R9 (Q9M6R9) Alpha-amylase , partial (58%)
Length = 1037
Score = 31.2 bits (69), Expect = 1.8
Identities = 23/91 (25%), Positives = 33/91 (35%)
Frame = +2
Query: 458 KWMKSPSWGSSTSTSFWKNTSTKGLVLSKNHVVADLSLTERAAKTSKQKSQVVEKTQATI 517
KW P+W + +FW V+ HV+ L R ++K +
Sbjct: 650 KWFMVPNWEGMDTINFWSQLCCVAQVVFCWHVMTLLHWNHR-----EEKGITCLYASGVL 814
Query: 518 DAATLKGIPSNIDLFKELIFPITLTAKNFEK 548
LK P + L+K L FP L A K
Sbjct: 815 SLIKLKLFP*SRRLWKAL*FPFNLRATQLNK 907
>TC227886 similar to UP|Q9M3Y5 (Q9M3Y5) 25.7 kDa protein, partial (87%)
Length = 1248
Score = 31.2 bits (69), Expect = 1.8
Identities = 28/129 (21%), Positives = 52/129 (39%), Gaps = 4/129 (3%)
Frame = +1
Query: 431 QPENRPSNEIADSSNHV-FDIDGSVYLRKWMKSPSWGSSTSTSFWKNTSTKGLVLSKNHV 489
QP+ + S+++ +NHV D + K +K S G +T + KN ++ G
Sbjct: 649 QPQKQSSSQVPKDANHVSVDNGANTVSAKVVKPSSGGKNTKGTPAKNVNSSGSFADAGQF 828
Query: 490 VADLSLTERAAKTSKQKSQVVEKTQATIDAATLKGIPSNIDL-FKEL--IFPITLTAKNF 546
V ++ S+ K + ++ + + N++ FK L IF T + F
Sbjct: 829 VEEILQKTSQLLLSEVKDENIKLHGEQLGTVLSDKLQKNLNAEFKSLAMIFKNTAYTEGF 1008
Query: 547 EKLRHWEEP 555
H+ P
Sbjct: 1009HAGTHYRPP 1035
>BU090294
Length = 416
Score = 30.0 bits (66), Expect = 4.0
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +2
Query: 564 LAYTLIFRNLLSYIFPVMLMITAVGMLTIRSLK 596
+AYTL F + L + P L+ITA+G+ IR L+
Sbjct: 230 IAYTLTFWSHLKFGLPSTLIITAIGLTLIR*LE 328
>TC218457 similar to PIR|T05166|T05166 quinone reductase homolog F18E5.200 -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(97%)
Length = 1052
Score = 30.0 bits (66), Expect = 4.0
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = -3
Query: 462 SPSWGSSTSTSFWKNTSTKGLVLS 485
SP+WGSSTS+S W + GLV++
Sbjct: 129 SPTWGSSTSSS-WSTSGPPGLVIT 61
>CO981629
Length = 844
Score = 29.3 bits (64), Expect = 6.8
Identities = 21/91 (23%), Positives = 41/91 (44%)
Frame = -1
Query: 562 LGLAYTLIFRNLLSYIFPVMLMITAVGMLTIRSLKEQGRLGRFFGGVMIRDQPPSNTIQK 621
+G+AYT++ ++ PVM+M+ TI LK G ++++ + +
Sbjct: 787 IGIAYTIV---IVLKTIPVMVMMLMATRTTIIMLKAMGIAMTIATAIVLKAVALATASET 617
Query: 622 IIAVKDAMRDVENMTQKVNVSLLKIRSILLS 652
+I +K + T V +LK +I L+
Sbjct: 616 VIVLKAIAMSMAITTAAARVMVLKAITITLA 524
>AW278165 similar to GP|14486705|gb phosphatidylinositol transfer-like
protein III {Lotus japonicus}, partial (22%)
Length = 427
Score = 29.3 bits (64), Expect = 6.8
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = -2
Query: 556 HLTVGFLGLAYTLIFRNLL 574
H T+G+ GLAYT+ F +LL
Sbjct: 192 HFTIGYTGLAYTITFLSLL 136
>TC214743 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane phosphoprotein
14 kDa, chloroplast precursor, partial (57%)
Length = 751
Score = 29.3 bits (64), Expect = 6.8
Identities = 22/74 (29%), Positives = 32/74 (42%), Gaps = 7/74 (9%)
Frame = +2
Query: 147 SLQVAWVTLQSKRVTEE-------AFVRIAPAVSGVVDRPTVHNLFKVLAGDKDGISMST 199
++Q AW ++ K A V A +S + P + + +V+ GI S
Sbjct: 320 TIQEAWDKVEDKYAVSSLAVAGVVALVGSAGMISAIDRLPLIPGVLEVV-----GIGYSG 484
Query: 200 WLAYINEFVKVRRE 213
WLAY N F K RE
Sbjct: 485 WLAYKNLFFKPDRE 526
>BI970752 similar to GP|10638981|emb protein phosphatase {Fagus sylvatica},
partial (24%)
Length = 537
Score = 29.3 bits (64), Expect = 6.8
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -1
Query: 118 PAFQRLIFITMLAWENPYTYVLSSN 142
PA QRL F T W+NPY +VL S+
Sbjct: 261 PAIQRLCF*TCHCWQNPY-FVLDSS 190
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.134 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,847,642
Number of Sequences: 63676
Number of extensions: 375738
Number of successful extensions: 2250
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 2226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2245
length of query: 754
length of database: 12,639,632
effective HSP length: 104
effective length of query: 650
effective length of database: 6,017,328
effective search space: 3911263200
effective search space used: 3911263200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC135799.9