BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135796.9 - phase: 0 /pseudo
(911 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227142 similar to UP|Q9FKF1 (Q9FKF1) DNA-directed RNA polymera... 540 e-153
TC227143 similar to UP|Q9FKF1 (Q9FKF1) DNA-directed RNA polymera... 171 2e-67
TC227141 similar to UP|Q9FKF1 (Q9FKF1) DNA-directed RNA polymera... 240 2e-63
BU927020 similar to GP|9759599|db DNA-directed RNA polymerase su... 217 2e-56
BI316187 87 1e-31
AI441634 similar to GP|9759599|dbj DNA-directed RNA polymerase s... 54 2e-07
TC217200 similar to UP|Q9LK40 (Q9LK40) DNA-directed RNA polymera... 40 0.004
BQ611779 similar to GP|8778315|gb|A F14J16.29 {Arabidopsis thali... 31 2.8
TC229269 similar to UP|O22958 (O22958) Expressed protein (At2g34... 29 8.2
TC204963 29 8.2
>TC227142 similar to UP|Q9FKF1 (Q9FKF1) DNA-directed RNA polymerase subunit,
partial (34%)
Length = 1626
Score = 540 bits (1390), Expect = e-153
Identities = 301/449 (67%), Positives = 341/449 (75%), Gaps = 5/449 (1%)
Frame = +1
Query: 468 ASHGKYSI*PAPTDGHLTLSTGLSSTTFTDNKIN*AGWV**AWSRSECNCSCHELQWL*Y 527
AS+GKYSI*PA DG L L T LSSTTF NK N*AGW+**AWSRS+C+ SC+EL WL*Y
Sbjct: 4 ASYGKYSI*PAAPDGQLALPTSLSSTTFAYNKDN*AGWI**AWSRSKCHSSCNEL*WL*Y 183
Query: 528 *GCNCHEQIIS*SRLWPLYSYEKVCRCPTKVFKWHRRSNRKAR*SFKQ---YADT***WD 584
C+CHEQ IS*S +W LYSYEKV ++ + H R N A *+ + YADT***W
Sbjct: 184 RRCHCHEQGIS*SWVWSLYSYEKV*CHHSEALE*HIRPNT*A**NCRHRRTYADT***WY 363
Query: 585 CFTWRNY*AN*HLYQQEITN*NT--E*SVVN*LA**CISV*LFNIQ*MSWR*SG**SSSL 642
C + N *A *HLYQ IT+* T + + + A *CIS IQ WR S ** S+
Sbjct: 364 CCSRGNS*AI*HLYQ*TITD*YTYSKNRICSKFAR*CISDQCTIIQ-G*WRRSS**GGSM 540
Query: 643 Q**G*QHVYQISNPSHSEARAWG*IQ*QTWAERCLRNNCPAGGLPIF*KRHLSRSDYESS 702
*G* HVYQISN SHS A AW *+ Q WA+RCL N CPA L IF*+RHLS SDYESS
Sbjct: 541 LG*G**HVYQISNSSHS*A*AW**V*QQAWAKRCLWNYCPARRLSIF*ERHLS*SDYESS 720
Query: 703 WVSKSNDSWKDDRASWRESRSFVW*ISLWQCFWGRKWSC*QG*NHK*NSCETWLQLQWER 762
W KSND WKDDRASWRESR F+W*+SLWQCFWG KWSC*QG*NHK*NSCE LQLQWER
Sbjct: 721 WFPKSNDCWKDDRASWRESRGFMW*VSLWQCFWGEKWSC*QG*NHK*NSCE*RLQLQWER 900
Query: 763 FHLFRYYGLPITSLYFYGANILPEIKTHGAG*DACSW*WP*SPDN*TANRRES*KWRSTS 822
FHLFRYYGLP S+YF+G+N+LPEIKT+GA *DACSW*WP N*TA RRES*KWRS+
Sbjct: 901 FHLFRYYGLPTASIYFHGSNLLPEIKTYGA**DACSW*WPSCHVN*TAYRRES*KWRSSC 1080
Query: 823 RRNGT*LLNCIWC*YVDF*ATHVI**SL*SSGL*SLWIVGILQP*VESWCLFIL*EWKAD 882
RNGT*LL+CIWC*YV+ *ATHV+**S *SSG+ +W+V ILQP* E+W LFIL*EW+
Sbjct: 1081WRNGT*LLDCIWC*YVNL*ATHVV**SF*SSGMYRMWVVRILQP*AENWYLFIL*EWRQY 1260
Query: 883 FHHEATICMQTLNSGTPINEHCSTLKTRR 911
F+HEATICMQ +SGTPINE+CS LKT R
Sbjct: 1261FYHEATICMQAHDSGTPINEYCSPLKTGR 1347
>TC227143 similar to UP|Q9FKF1 (Q9FKF1) DNA-directed RNA polymerase subunit,
partial (13%)
Length = 663
Score = 171 bits (433), Expect(2) = 2e-67
Identities = 88/121 (72%), Positives = 100/121 (81%)
Frame = +3
Query: 791 GAG*DACSW*WP*SPDN*TANRRES*KWRSTSRRNGT*LLNCIWC*YVDF*ATHVI**SL 850
GA *DACSW*WP N*TA RRES*KWRS+ RNGT*LL+CIWC*YV+ *ATHV+**S
Sbjct: 165 GA**DACSW*WPSCHVN*TAYRRES*KWRSSCWRNGT*LLDCIWC*YVNL*ATHVV**SF 344
Query: 851 *SSGL*SLWIVGILQP*VESWCLFIL*EWKADFHHEATICMQTLNSGTPINEHCSTLKTR 910
*SSG+ +W+V ILQP* E+W LFIL*EW+ F+HEATICMQ +SGTPINE+CS LKT
Sbjct: 345 *SSGMYRMWVVRILQP*AENWYLFIL*EWRQYFYHEATICMQAHDSGTPINEYCSPLKTG 524
Query: 911 R 911
R
Sbjct: 525 R 527
Score = 104 bits (259), Expect(2) = 2e-67
Identities = 47/55 (85%), Positives = 49/55 (88%)
Frame = +2
Query: 713 DDRASWRESRSFVW*ISLWQCFWGRKWSC*QG*NHK*NSCETWLQLQWERFHLFR 767
DDRASW ESR F+W*+SL QCFWG KWSC*QG*NHK*NSCE LQLQWERFHLFR
Sbjct: 2 DDRASWGESRGFMW*VSLRQCFWGEKWSC*QG*NHK*NSCE*RLQLQWERFHLFR 166
>TC227141 similar to UP|Q9FKF1 (Q9FKF1) DNA-directed RNA polymerase subunit,
partial (12%)
Length = 773
Score = 240 bits (612), Expect = 2e-63
Identities = 117/161 (72%), Positives = 136/161 (83%)
Frame = +2
Query: 751 SCETWLQLQWERFHLFRYYGLPITSLYFYGANILPEIKTHGAG*DACSW*WP*SPDN*TA 810
SCE L LQWERFHLFRYYG+P S+YF+G+N+LPEIKT+GA *DAC+W*WP N TA
Sbjct: 2 SCEXXLHLQWERFHLFRYYGMPSASIYFHGSNLLPEIKTYGA**DACTW*WPSCHVNQTA 181
Query: 811 NRRES*KWRSTSRRNGT*LLNCIWC*YVDF*ATHVI**SL*SSGL*SLWIVGILQP*VES 870
RRES*KWRS+ RNGT*LL+CIWC*YV+ *ATHV+**S *SSG+ S+W+VGILQP* E+
Sbjct: 182 YRRES*KWRSSCWRNGT*LLDCIWC*YVNL*ATHVV**SF*SSGMYSMWVVGILQP*AEN 361
Query: 871 WCLFIL*EWKADFHHEATICMQTLNSGTPINEHCSTLKTRR 911
W LFIL*EW+ F+HEA ICMQT +SGTPINE+CS LKT R
Sbjct: 362 WYLFIL*EWRQYFYHEAAICMQTHDSGTPINEYCSPLKTGR 484
>BU927020 similar to GP|9759599|db DNA-directed RNA polymerase subunit
{Arabidopsis thaliana}, partial (12%)
Length = 444
Score = 217 bits (553), Expect = 2e-56
Identities = 111/147 (75%), Positives = 121/147 (81%)
Frame = +2
Query: 237 EV*ESKWP*SFAA*PVGHALSL*YS*R*ILWTSKEPGFDDSCHY**RGGPPDFSVLSFGC 296
EV ESKW *SFAA*PVGHALSL*Y *R*+LWTSKE GFDDSC++**RG DFSV FG
Sbjct: 2 EVQESKWT*SFAA*PVGHALSL*YP*R*VLWTSKELGFDDSCYH**RGEALDFSVRVFGR 181
Query: 297 *KHGTSIWRRASYTQFISCHF*WYDPWQAQETTAFCYCNEKVAESLSNWRVCECLCQ*KA 356
*+H S+ R ASYT+F+SCHF*W++PWQA E TAFCYCN KVAES NW VC CLCQ*KA
Sbjct: 182 *RHERSLCRGASYTRFLSCHF*WFNPWQA*EATAFCYCN*KVAESW*NW*VCVCLCQ*KA 361
Query: 357 VLCLLSFRWW*SLPSSRHC*QWNIKSQ 383
LCLLSF WW*SLPS HC*Q NIK+Q
Sbjct: 362 ALCLLSF*WW*SLPSPGHC*QGNIKNQ 442
>BI316187
Length = 406
Score = 87.0 bits (214), Expect(2) = 1e-31
Identities = 39/55 (70%), Positives = 46/55 (82%)
Frame = +2
Query: 857 SLWIVGILQP*VESWCLFIL*EWKADFHHEATICMQTLNSGTPINEHCSTLKTRR 911
S+W+ GILQP* E+W LFIL*EW+ F+HEA ICMQT +SGTPINE+CS LKT R
Sbjct: 137 SMWVAGILQP*AENWYLFIL*EWRQYFYHEAAICMQTHDSGTPINEYCSPLKTGR 301
Score = 69.3 bits (168), Expect(2) = 1e-31
Identities = 33/44 (75%), Positives = 36/44 (81%)
Frame = +1
Query: 795 DACSW*WP*SPDN*TANRRES*KWRSTSRRNGT*LLNCIWC*YV 838
DAC+W*WP N TA RRES*KWRS+ RNGT*LL+CIWC*YV
Sbjct: 4 DACTW*WPSCHVNQTAYRRES*KWRSSCWRNGT*LLDCIWC*YV 135
>AI441634 similar to GP|9759599|dbj DNA-directed RNA polymerase subunit
{Arabidopsis thaliana}, partial (2%)
Length = 414
Score = 54.3 bits (129), Expect = 2e-07
Identities = 26/33 (78%), Positives = 26/33 (78%)
Frame = +3
Query: 324 QAQETTAFCYCNEKVAESLSNWRVCECLCQ*KA 356
QA E TAFCYCN KVAES NW VC CLCQ*KA
Sbjct: 6 QA*EATAFCYCN*KVAESW*NW*VCVCLCQ*KA 104
>TC217200 similar to UP|Q9LK40 (Q9LK40) DNA-directed RNA polymerase, subunit
B, partial (27%)
Length = 1200
Score = 40.4 bits (93), Expect = 0.004
Identities = 43/184 (23%), Positives = 72/184 (38%), Gaps = 7/184 (3%)
Frame = +1
Query: 659 SEARAWG*IQ*QTWAERCLRNNCPAGGLPIF*KRHLSRSDYESSWVSKSNDSWKDDRASW 718
S++R+W + W++ + +G RH S ++S+ +S + +SW R +
Sbjct: 220 SKSRSWRQVLQYAWSKGSFGISGVSGKFSFHKTRHCS*YCHKSACISLTTNSWATPRGCF 399
Query: 719 RESRSFVW*ISLWQCFWGRKWSC*QG*NHK*NSCETWL-------QLQWERFHLFRYYGL 771
R+ W F C H * + ++W+ +QW+ +
Sbjct: 400 RKGDCLWWYTETSYAFLHSFC*C-----HY*TASQSWVFKMGK*EGIQWKDW*------- 543
Query: 772 PITSLYFYGANILPEIKTHGAG*DACSW*WP*SPDN*TANRRES*KWRSTSRRNGT*LLN 831
+ +FYG NILP + W SP + TA + WR R +G *L N
Sbjct: 544 DGSITHFYGTNILPTSAPYV*RQSEIPEHWSSSPVDPTACS*QKTIWRYQVR*DGA*LPN 723
Query: 832 CIWC 835
WC
Sbjct: 724 SSWC 735
>BQ611779 similar to GP|8778315|gb|A F14J16.29 {Arabidopsis thaliana},
partial (30%)
Length = 445
Score = 30.8 bits (68), Expect = 2.8
Identities = 16/41 (39%), Positives = 18/41 (43%)
Frame = -3
Query: 712 KDDRASWRESRSFVW*ISLWQCFWGRKWSC*QG*NHK*NSC 752
+D WR+S W* LWQC W C QG N C
Sbjct: 254 RDAAGKWRQSGRQRW*GRLWQC-----WDCSQGWNIALRQC 147
>TC229269 similar to UP|O22958 (O22958) Expressed protein
(At2g34050/T14G11.17), partial (79%)
Length = 1105
Score = 29.3 bits (64), Expect = 8.2
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = -3
Query: 177 FLWMCL*QLINRDCITPGLERTLSTGNF 204
FLW C Q + + C+ GLE+TLS +F
Sbjct: 560 FLWPCNLQDL*KSCVASGLEQTLSCIHF 477
>TC204963
Length = 1354
Score = 29.3 bits (64), Expect = 8.2
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = -2
Query: 445 CGINSISSS*SISQKHISVCNGKASHGKYSI*PAPTDGHLTL 486
CG +ISS + +Q +S+ N KAS K+ +* P D HL +
Sbjct: 957 CGSYNISSCDNPNQSTLSIYNWKAS--KFGL*QQPKDRHLKM 838
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.365 0.160 0.668
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,445,756
Number of Sequences: 63676
Number of extensions: 903020
Number of successful extensions: 11260
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 4478
Number of HSP's successfully gapped in prelim test: 471
Number of HSP's that attempted gapping in prelim test: 6458
Number of HSP's gapped (non-prelim): 5538
length of query: 911
length of database: 12,639,632
effective HSP length: 106
effective length of query: 805
effective length of database: 5,889,976
effective search space: 4741430680
effective search space used: 4741430680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC135796.9