BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135795.3 + phase: 0
(134 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CD400517 30 0.44
TC232379 similar to UP|Q9SWV4 (Q9SWV4) ER66 protein (Fragment), ... 28 0.98
BG043098 28 0.98
TC208701 weakly similar to UP|Q9SAE5 (Q9SAE5) F3F19.14 protein, ... 28 1.3
TC231321 similar to UP|Q6NPP4 (Q6NPP4) At5g64220, partial (8%) 27 2.9
TC225793 similar to GB|AAM65667.1|21593700|AY088122 J8-like prot... 26 4.9
BE801380 26 4.9
TC206381 homologue to UP|Q9ZNX1 (Q9ZNX1) NAD-dependent isocitrat... 26 4.9
CD390549 26 6.4
TC230093 similar to UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-l... 26 6.4
TC225790 similar to GB|AAM65667.1|21593700|AY088122 J8-like prot... 26 6.4
TC207227 similar to UP|Q6L610 (Q6L610) Gamma-glutamyl transferas... 25 8.3
TC218684 weakly similar to UP|Q9LHB1 (Q9LHB1) Transcription fact... 25 8.3
>CD400517
Length = 561
Score = 29.6 bits (65), Expect = 0.44
Identities = 25/72 (34%), Positives = 38/72 (52%), Gaps = 4/72 (5%)
Frame = -2
Query: 65 GAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYET--EEEEDMEILDKL--EKLESAL 120
G +RL+E+ I N + + H YE+ EE+E +EI K +KLE A+
Sbjct: 512 GTPSRLEEI-------IASLNCFRVLYAPQGPHRYESRGEEQETLEIRRKWLRKKLERAM 354
Query: 121 QLILEILVIKKK 132
+++ EILV KK
Sbjct: 353 KVLSEILVWPKK 318
>TC232379 similar to UP|Q9SWV4 (Q9SWV4) ER66 protein (Fragment), partial
(34%)
Length = 828
Score = 28.5 bits (62), Expect = 0.98
Identities = 15/53 (28%), Positives = 30/53 (56%)
Frame = +3
Query: 20 QNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDE 72
Q +Q ++ ALA VK + V++ + D+Y LNV+ + + ++ E + +E
Sbjct: 519 QTEQRLQKALARVKSM-VQYPEARDQYHRLLNVVTEIQENQVKHESSYNNSEE 674
>BG043098
Length = 411
Score = 28.5 bits (62), Expect = 0.98
Identities = 29/95 (30%), Positives = 44/95 (45%), Gaps = 15/95 (15%)
Frame = +2
Query: 52 VMNDFKTLRI------DAEGAKARLDE-----LFKEHRHLIMRFNSLTLKGSH----FTK 96
V+N F++ RI D+EG K +L + L L++ + L +H FTK
Sbjct: 74 VVNCFESFRISWYASIDSEGIKPKLYQSLYVILALVILRLLICLSRTLLMCTHDCCIFTK 253
Query: 97 HGYETEEEEDMEILDKLEKLESALQLILEILVIKK 131
HGY T + L+ L + LQ IL + + KK
Sbjct: 254 HGYYTVSLASSTV---LQSLHNRLQKILLLNIFKK 349
>TC208701 weakly similar to UP|Q9SAE5 (Q9SAE5) F3F19.14 protein, partial
(19%)
Length = 951
Score = 28.1 bits (61), Expect = 1.3
Identities = 12/45 (26%), Positives = 27/45 (59%)
Frame = +1
Query: 78 RHLIMRFNSLTLKGSHFTKHGYETEEEEDMEILDKLEKLESALQL 122
+HL+ + ++ K ++F GY ++E+ D L++LES +++
Sbjct: 451 KHLVYKKSAYQSKEAYFRSIGYREDDEKMESTEDYLKRLESYMKM 585
>TC231321 similar to UP|Q6NPP4 (Q6NPP4) At5g64220, partial (8%)
Length = 616
Score = 26.9 bits (58), Expect = 2.9
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = +1
Query: 20 QNQQSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEG 65
Q ++ ++ AL+ VK + ++ + +Y LNV+ DF+ + EG
Sbjct: 181 QKEEKIQKALSRVKSM-AQYPEARAQYRRLLNVVEDFRQTKASNEG 315
>TC225793 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;} , partial (58%)
Length = 1345
Score = 26.2 bits (56), Expect = 4.9
Identities = 18/68 (26%), Positives = 36/68 (52%)
Frame = +1
Query: 46 YDEFLNVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYETEEEE 105
Y +V++ +KTLRI +++ E+ K R L ++++ +GS+ +E E
Sbjct: 490 YSSSSSVIDPYKTLRIKRGASES---EVRKAFRQLALQYHPDVCRGSNCGVQFHEINEAY 660
Query: 106 DMEILDKL 113
D+ ++ KL
Sbjct: 661 DV-VMSKL 681
>BE801380
Length = 417
Score = 26.2 bits (56), Expect = 4.9
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +2
Query: 23 QSMKDALAFVKGVEVEFEDKGDKYDEFLNVMNDFKTLRIDAEGAKARLDE 72
+++KD L+ V+ V ++ + K +K E + K + DAE LDE
Sbjct: 8 KNLKDTLSLVQAVLLDADQKQEKNHELREWLRQLKRVFFDAENV---LDE 148
>TC206381 homologue to UP|Q9ZNX1 (Q9ZNX1) NAD-dependent isocitrate
dehydrogenase precursor , partial (67%)
Length = 929
Score = 26.2 bits (56), Expect = 4.9
Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = -3
Query: 52 VMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKH-GYETEEEEDMEIL 110
V+ D + I KAR E + I F L ++G+ T H G E+ E D+ +
Sbjct: 513 VLPDDNDIHIIMARGKAREIETINKRSIEIKLFPELNIEGADATAHGGNESSLETDLVLP 334
Query: 111 DKLEKL 116
+++E L
Sbjct: 333 NRIEDL 316
>CD390549
Length = 481
Score = 25.8 bits (55), Expect = 6.4
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = +2
Query: 88 TLKGSHFTKHGYETEEEEDMEILDKLEKLESALQLILEILVIKK 131
T+ HF + EE+ED + + + ++ + +L+I VI+K
Sbjct: 5 TI*SKHFHLPANQYEEQEDDKRMTNINQISD*IMYLLQIKVIRK 136
>TC230093 similar to UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-like protein,
partial (96%)
Length = 1196
Score = 25.8 bits (55), Expect = 6.4
Identities = 13/20 (65%), Positives = 15/20 (75%), Gaps = 1/20 (5%)
Frame = -2
Query: 84 FNSLTLKGSHFTKHG-YETE 102
F+SL L G+HF KHG Y TE
Sbjct: 775 FHSLYLGGNHFQKHGAY*TE 716
>TC225790 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;} , partial (59%)
Length = 1000
Score = 25.8 bits (55), Expect = 6.4
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +2
Query: 51 NVMNDFKTLRIDAEGAKARLDELFKEHRHLIMRFNSLTLKGSHFTKHGYETEEEED 106
+VM+ +KTLRI +++ E+ K R L ++++ +GS+ +E E D
Sbjct: 335 SVMDPYKTLRIQRGASES---EVRKAFRQLALQYHPDVCRGSNCGVQFHEINEAYD 493
>TC207227 similar to UP|Q6L610 (Q6L610) Gamma-glutamyl transferase, partial
(26%)
Length = 815
Score = 25.4 bits (54), Expect = 8.3
Identities = 13/51 (25%), Positives = 28/51 (54%)
Frame = +2
Query: 72 ELFKEHRHLIMRFNSLTLKGSHFTKHGYETEEEEDMEILDKLEKLESALQL 122
++ K+HRH FN L + H + ++E D+ I+ L+ +++ ++L
Sbjct: 662 KIVKKHRHFKTIFNFLAMLYQHHPGQKKKKKKENDLVII--LQTIKNPMKL 808
>TC218684 weakly similar to UP|Q9LHB1 (Q9LHB1) Transcription factor X1-like
protein, partial (21%)
Length = 1041
Score = 25.4 bits (54), Expect = 8.3
Identities = 19/57 (33%), Positives = 32/57 (55%), Gaps = 11/57 (19%)
Frame = +2
Query: 89 LKGS-HFTKHGYETEEEEDMEILDKLEKLESALQ----------LILEILVIKKKEA 134
LKGS KH E++ED EIL+K++ L+ L+ + + L+IK++E+
Sbjct: 11 LKGSLSVLKH---MEDDEDAEILNKVDTLQKDLRDKEQSLQDLHALNQTLIIKERES 172
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.136 0.363
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,941,178
Number of Sequences: 63676
Number of extensions: 37214
Number of successful extensions: 252
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 251
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of query: 134
length of database: 12,639,632
effective HSP length: 87
effective length of query: 47
effective length of database: 7,099,820
effective search space: 333691540
effective search space used: 333691540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC135795.3