BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.3 + phase: 0
(166 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis ... 181 2e-46
BU549069 178 9e-46
TC211627 77 4e-15
TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 70 5e-13
NP595172 polyprotein [Glycine max] 66 7e-12
TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 66 9e-12
TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, parti... 65 1e-11
TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 57 4e-09
BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza... 54 3e-08
TC232772 weakly similar to UP|Q9LQH2 (Q9LQH2) F15O4.13, partial ... 35 0.017
BE807407 33 0.063
TC211469 32 0.18
BU578866 31 0.31
TC205176 similar to UP|AROC_CORSE (P27793) Chorismate synthase, ... 28 2.0
BE331168 similar to SP|O49849|GCST Aminomethyltransferase mitoc... 28 2.0
AW234303 28 2.6
TC225715 similar to GB|AAM16218.1|20334714|AY093957 At2g41900/T6... 28 2.6
TC227413 similar to UP|Q7PS25 (Q7PS25) ENSANGP00000020753 (Fragm... 27 4.5
TC203691 magmas-like protein [Glycine max] 26 7.7
BE022855 similar to GP|14190487|gb| AT3g59280/F25L23_140 {Arabid... 26 7.7
>BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis melo},
partial (9%)
Length = 410
Score = 181 bits (458), Expect = 2e-46
Identities = 88/136 (64%), Positives = 108/136 (78%)
Frame = -3
Query: 15 SYHDKRRKALEFQEGDHVFLRVTPMTGVRRALKSRKLTPKFIGPYQISERVGTVAYRVGL 74
SYHDKRRK LEF+ GDHVFLRVTP TGV RALKS KLTP FIGP+QI ++V VAY++ L
Sbjct: 408 SYHDKRRKDLEFEVGDHVFLRVTP*TGVGRALKS*KLTPHFIGPFQILKKVDFVAYQIAL 229
Query: 75 PTHLSNLHDVFHVSQLQKYVADPSHVIPREDVQVRDNLTVETSPLRIEDREVKKLRGKDI 134
P L++LH+VFHVSQ+ KY+ DPSH++ + VQV++NL ET PLRI+D K LRGK+I
Sbjct: 228 PPSLTSLHNVFHVSQIHKYINDPSHMVNLDVVQVKENLAHETLPLRIKDMRTKHLRGKEI 49
Query: 135 PLVKVVWGGVTGESLT 150
LVKV+WGG +GE T
Sbjct: 48 LLVKVIWGGASGEEAT 1
>BU549069
Length = 615
Score = 178 bits (452), Expect = 9e-46
Identities = 86/132 (65%), Positives = 104/132 (78%)
Frame = -1
Query: 34 LRVTPMTGVRRALKSRKLTPKFIGPYQISERVGTVAYRVGLPTHLSNLHDVFHVSQLQKY 93
L+VTP TGV +ALKSRKLTP FIG +QI +R VAY++ LP LSNLH+VFHVSQL+ Y
Sbjct: 615 LKVTPRTGVGQALKSRKLTPHFIGHFQILKRAXPVAYQIALPPSLSNLHNVFHVSQLRMY 436
Query: 94 VADPSHVIPREDVQVRDNLTVETSPLRIEDREVKKLRGKDIPLVKVVWGGVTGESLTWEL 153
+ DPSHV+ +DVQV++NLT ET PLRIEDR K LR K+ PLVKV+WGG +GE TWEL
Sbjct: 435 IHDPSHVVKLDDVQVKENLTYETLPLRIEDRRTKHLRRKENPLVKVIWGGTSGEDATWEL 256
Query: 154 ESKMRDSYPESF 165
ES+MR +YP F
Sbjct: 255 ESQMRVAYPSLF 220
>TC211627
Length = 1034
Score = 77.0 bits (188), Expect = 4e-15
Identities = 50/158 (31%), Positives = 78/158 (48%), Gaps = 1/158 (0%)
Frame = +3
Query: 5 KMKASHSRQKSYHDKRRKALEFQEGDHVFLRVTPMTGVRRALKSRKLTPKFIGPYQISER 64
+++ K D R+ L F GD V++R+ P KL+ +F GPYQI R
Sbjct: 357 RLQKYQDSMKRIADSHRRDLTFNIGDWVYVRL*PYRQTSIQSTYTKLSKRFYGPYQIQAR 536
Query: 65 VGTVAYRVGLPTHLSNLHDVFHVSQLQKYVAD-PSHVIPREDVQVRDNLTVETSPLRIED 123
VG VAYR+ LP S +H +FHVS L+ + P ++ ++ V+ PL+ D
Sbjct: 537 VGQVAYRLQLPP-TSKIHPIFHVSLLKVHHGPIPPELLALPPFSTTNHPLVQ--PLQFLD 707
Query: 124 REVKKLRGKDIPLVKVVWGGVTGESLTWELESKMRDSY 161
++ + IP V V W + E TWE ++++D Y
Sbjct: 708 WKMDESTTPPIPQVLVQWTNLAPEDTTWESWTQLKDIY 821
>TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 730
Score = 70.1 bits (170), Expect = 5e-13
Identities = 39/94 (41%), Positives = 61/94 (64%), Gaps = 3/94 (3%)
Frame = +1
Query: 2 IREKMKASHSRQKSYHDKRRKALEFQEGDHVFLRVTPMTGVRRALKSR---KLTPKFIGP 58
+RE + S + +KRR+ +E+ GD VFL++ P RR+L R KL+P+F P
Sbjct: 64 LRENLLKS*DIM*ANTNKRRRDIEYVVGD*VFLKMQPYR--RRSLAKRINEKLSPRFYAP 237
Query: 59 YQISERVGTVAYRVGLPTHLSNLHDVFHVSQLQK 92
+Q+ +VGT+AY++ LP+H+ +H VFHVS L+K
Sbjct: 238 FQVFNKVGTIAYKLDLPSHI-KIHPVFHVSLLKK 336
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 66.2 bits (160), Expect = 7e-12
Identities = 44/153 (28%), Positives = 77/153 (49%), Gaps = 4/153 (2%)
Frame = +1
Query: 14 KSYHDKRRKALEFQEGDHVFLRVTPMTGVRRAL-KSRKLTPKFIGPYQISERVGTVAYRV 72
K DK+R + FQ GD V +++ P L K++KL+ ++ GP+++ ++G VAY++
Sbjct: 4018 KRQADKKRLDVSFQIGDEVLVKLQPYRQHSAVLRKNQKLSMRYFGPFKVLAKIGDVAYKL 4197
Query: 73 GLPTHLSNLHDVFHVSQLQKY---VADPSHVIPREDVQVRDNLTVETSPLRIEDREVKKL 129
LP+ + +H VFHVSQL+ + DP +P ++ + P++I +
Sbjct: 4198 ELPS-AARIHPVFHVSQLKPFNGTAQDPYLPLPLTVTEMGPVM----QPVKILASRIIIR 4362
Query: 130 RGKDIPLVKVVWGGVTGESLTWELESKMRDSYP 162
I + V W + TWE ++ SYP
Sbjct: 4363 GHNQIEQILVQWENGLQDEATWEDIEDIKASYP 4461
>TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (5%)
Length = 761
Score = 65.9 bits (159), Expect = 9e-12
Identities = 41/136 (30%), Positives = 68/136 (49%), Gaps = 3/136 (2%)
Frame = +1
Query: 29 GDHVFLRVTP-MTGVRRALKSRKLTPKFIGPYQISERVGTVAYRVGLPTHLSNLHDVFHV 87
GD V +++ P G KLT ++ GP+++ ER+G V YR+ L H S +H VFHV
Sbjct: 7 GDWVLVKLRPHRQGSASETTYSKLTKRYYGPFEVQERLGKVVYRLKLTAH-SRIHPVFHV 183
Query: 88 SQLQKYVADP--SHVIPREDVQVRDNLTVETSPLRIEDREVKKLRGKDIPLVKVVWGGVT 145
S L+ +V DP +H P ++ + +PL + D ++ +V V W +
Sbjct: 184 SLLKAFVGDPETTHAGPLPVMRTEE---ATNTPLTVIDSKLVPADNGPRRMVLVQWPSAS 354
Query: 146 GESLTWELESKMRDSY 161
+ +WE +R+ Y
Sbjct: 355 RQDASWEDWQVLRERY 402
>TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, partial (7%)
Length = 810
Score = 65.5 bits (158), Expect = 1e-11
Identities = 36/90 (40%), Positives = 55/90 (61%), Gaps = 1/90 (1%)
Frame = +3
Query: 14 KSYHDKRRKALEFQEGDHVFLRVTPMTGVRRALK-SRKLTPKFIGPYQISERVGTVAYRV 72
K+Y D+ R+A+ GD V+L++ P A K + KL+P+F GPYQI +++G VA+ +
Sbjct: 6 KAYADRSRRAVTLSVGDWVYLKLQPYRLKSLAKKRNEKLSPRFYGPYQIKKQIGLVAFEL 185
Query: 73 GLPTHLSNLHDVFHVSQLQKYVADPSHVIP 102
LP +H VFH S L+K VA ++ P
Sbjct: 186 DLPP-ARKIHPVFHASLLKKAVAATANPQP 272
>TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 664
Score = 57.0 bits (136), Expect = 4e-09
Identities = 38/103 (36%), Positives = 56/103 (53%)
Frame = +2
Query: 50 KLTPKFIGPYQISERVGTVAYRVGLPTHLSNLHDVFHVSQLQKYVADPSHVIPREDVQVR 109
KL+ +F GP+Q+ ERVG VAYR+ LP + LH VFH S L+ + +P +
Sbjct: 74 KLSKRFFGPFQVVERVGKVAYRLQLPVD-AKLHPVFHCSLLKPFQGNPPDTAAPLPPTLF 250
Query: 110 DNLTVETSPLRIEDREVKKLRGKDIPLVKVVWGGVTGESLTWE 152
D+ +V +PL I + + DI V V W G++ + TWE
Sbjct: 251 DHQSV-IAPLVI--LATRTVNDDDIE-VLVQWQGLSPDDATWE 367
>BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza sativa
(japonica cultivar-group)}, partial (3%)
Length = 424
Score = 54.3 bits (129), Expect = 3e-08
Identities = 39/127 (30%), Positives = 67/127 (52%), Gaps = 4/127 (3%)
Frame = +1
Query: 6 MKASHSRQKSYHDKRRKALEFQEGDHVFLRVTP-MTGVRRALKSRKLTPKFIGPYQISER 64
++ + S + + R+ + GD V+L++ P G KLT +F GPY + +
Sbjct: 25 LERAQSLMVKHANNHRRPHDINVGDWVYLKIRPHRQGSMPPRLHPKLTARFYGPYLVMRQ 204
Query: 65 VGTVAYRVGLPTHLSNLHDVFHVSQLQKYVADPSHVIPRE---DVQVRDNLTVETSPLRI 121
VG VA+++ LP+ + +H VFHVSQL++ + +H E D++ + L L+I
Sbjct: 205 VGAVAFQLQLPSE-ARIHPVFHVSQLKRALG--NHQAQEELPPDLEHQAELYFPVQILKI 375
Query: 122 EDREVKK 128
REV+K
Sbjct: 376 --REVQK 390
>TC232772 weakly similar to UP|Q9LQH2 (Q9LQH2) F15O4.13, partial (7%)
Length = 729
Score = 35.0 bits (79), Expect = 0.017
Identities = 20/78 (25%), Positives = 41/78 (51%)
Frame = +1
Query: 18 DKRRKALEFQEGDHVFLRVTPMTGVRRALKSRKLTPKFIGPYQISERVGTVAYRVGLPTH 77
+K RK + + D V++ + ++ + KL P+ GP+Q+ ER+ AY++ +P
Sbjct: 241 NKNRKKVVLEPSDWVWVHMRKERFPKQRMS--KLQPRGDGPFQVLERINYNAYKIDIPGE 414
Query: 78 LSNLHDVFHVSQLQKYVA 95
+ F+++ L +VA
Sbjct: 415 Y-EVSSSFNIADLTPFVA 465
>BE807407
Length = 341
Score = 33.1 bits (74), Expect = 0.063
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 50 KLTPKFIGPYQISERVGTVAYRVGLPTH 77
KLT ++ GPY + +VG VA+++ LP H
Sbjct: 158 KLTARYYGPYLVLIKVGVVAFQLQLPKH 241
>TC211469
Length = 480
Score = 31.6 bits (70), Expect = 0.18
Identities = 22/67 (32%), Positives = 32/67 (46%)
Frame = +2
Query: 95 ADPSHVIPREDVQVRDNLTVETSPLRIEDREVKKLRGKDIPLVKVVWGGVTGESLTWELE 154
A PS +IP + V + V +PL I D ++ LV V W G+ E +WEL
Sbjct: 14 APPSDIIPLPPLAVDHHPVV--TPLTILDWKLDSSVTPPRRLVLVQWMGLPPEDTSWELW 187
Query: 155 SKMRDSY 161
++ SY
Sbjct: 188 DDLQQSY 208
>BU578866
Length = 409
Score = 30.8 bits (68), Expect = 0.31
Identities = 17/58 (29%), Positives = 28/58 (47%)
Frame = +2
Query: 78 LSNLHDVFHVSQLQKYVADPSHVIPREDVQVRDNLTVETSPLRIEDREVKKLRGKDIP 135
LSN+HDV S L + P V DV N +++ L R +++L +++P
Sbjct: 212 LSNMHDVVSTSSLDLALVKPLPVSFPSDVSTGKNTNIDSVQLTQATRPIRRLYLENLP 385
>TC205176 similar to UP|AROC_CORSE (P27793) Chorismate synthase, chloroplast
precursor (5-enolpyruvylshikimate-3-phosphate
phospholyase) , partial (25%)
Length = 698
Score = 28.1 bits (61), Expect = 2.0
Identities = 20/62 (32%), Positives = 31/62 (49%), Gaps = 1/62 (1%)
Frame = -3
Query: 81 LHDVFHVSQLQKYVADPSHVIPREDVQ-VRDNLTVETSPLRIEDREVKKLRGKDIPLVKV 139
LH + H S +Y P + ED VRD + RI DR+ +K R ++ L+++
Sbjct: 450 LHMLKHESDFHRYSQQPVSEVSWEDEDGVRDG---GSRGRRIGDRD*EKRRRRERRLMRM 280
Query: 140 VW 141
VW
Sbjct: 279 VW 274
>BE331168 similar to SP|O49849|GCST Aminomethyltransferase mitochondrial
precursor (EC 2.1.2.10) (Glycine cleavage system T
protein), partial (27%)
Length = 430
Score = 28.1 bits (61), Expect = 2.0
Identities = 15/48 (31%), Positives = 26/48 (53%), Gaps = 1/48 (2%)
Frame = -1
Query: 37 TPMTGVRRALK-SRKLTPKFIGPYQISERVGTVAYRVGLPTHLSNLHD 83
TP + +L+ S + P +IG YQ+ + G+V PTH+ N+ +
Sbjct: 379 TPFSFCEHSLRPSCRGKPAYIGNYQLFKLCGSVLPSEDQPTHMGNIKE 236
>AW234303
Length = 398
Score = 27.7 bits (60), Expect = 2.6
Identities = 13/31 (41%), Positives = 18/31 (57%), Gaps = 1/31 (3%)
Frame = +1
Query: 17 HDKRRKALEF-QEGDHVFLRVTPMTGVRRAL 46
HDK K ++ QEG HV+ + + RRAL
Sbjct: 274 HDKTSKGQDYYQEGSHVYAELNVLEAFRRAL 366
>TC225715 similar to GB|AAM16218.1|20334714|AY093957 At2g41900/T6D20.20
{Arabidopsis thaliana;} , partial (42%)
Length = 2142
Score = 27.7 bits (60), Expect = 2.6
Identities = 14/48 (29%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = -3
Query: 15 SYHDKRRKALEFQEGDHVFLRVT-PMTGVRRALKSRKLTPKFIGPYQI 61
+Y+ K+ + +E+ G+ + + P T + L S+K++PK PY+I
Sbjct: 1669 NYYLKKEEKIEYPSGNFAANQSSIPFTTLLSLLSSQKISPKEYVPYEI 1526
>TC227413 similar to UP|Q7PS25 (Q7PS25) ENSANGP00000020753 (Fragment),
partial (38%)
Length = 1408
Score = 26.9 bits (58), Expect = 4.5
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = +1
Query: 113 TVETSPLRIEDREVKKLRGKDIPLVKVVWGGVTGES 148
TV + ED +R D+ V VV+GGVTG S
Sbjct: 598 TVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYS 705
>TC203691 magmas-like protein [Glycine max]
Length = 698
Score = 26.2 bits (56), Expect = 7.7
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +1
Query: 32 VFLRVTPMTGVRRALKSRKLTPKFIGPYQISERVGTVAYRVGLPTHLSNLH 82
++LR+ GV + +PKFIGP + + RV L +L LH
Sbjct: 484 IYLRIMLRMGVSTS------SPKFIGPRNV*RQFNKARVRVPLVENLDFLH 618
>BE022855 similar to GP|14190487|gb| AT3g59280/F25L23_140 {Arabidopsis
thaliana}, partial (33%)
Length = 411
Score = 26.2 bits (56), Expect = 7.7
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +1
Query: 32 VFLRVTPMTGVRRALKSRKLTPKFIGPYQISERVGTVAYRVGLPTHLSNLH 82
++LR+ GV + +PKFIGP + + RV L +L LH
Sbjct: 7 IYLRIMLRMGVSTS------SPKFIGPRNV*RQFNKARVRVPLVENLDFLH 141
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,276,769
Number of Sequences: 63676
Number of extensions: 72523
Number of successful extensions: 362
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 355
length of query: 166
length of database: 12,639,632
effective HSP length: 90
effective length of query: 76
effective length of database: 6,908,792
effective search space: 525068192
effective search space used: 525068192
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC135504.3