BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.12 - phase: 0
(179 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC223727 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 135 8e-33
BI316922 117 2e-27
BI321666 85 2e-17
AW184779 67 3e-14
NP595172 polyprotein [Glycine max] 55 2e-08
TC212032 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 54 3e-08
CO982196 50 6e-07
TC206741 40 8e-04
BE801213 weakly similar to GP|6691193|gb| F7F22.17 {Arabidopsis ... 33 0.094
TC219268 32 0.16
AW507721 weakly similar to GP|27764548|gb polyprotein {Glycine m... 31 0.27
TC219333 homologue to UP|Q42437 (Q42437) HD-ZIP protein, partial... 28 2.3
TC203529 UP|Q6Y1F1 (Q6Y1F1) Alpha-tubulin (Fragment), partial (26%) 28 3.0
BM308155 weakly similar to GP|14279688|gb receptor-like kinase X... 27 4.0
TC224002 weakly similar to UP|Q9LG26 (Q9LG26) F14J16.11, partial... 27 4.0
BI786481 similar to GP|10177293|db ATP-dependent RNA helicase-li... 27 5.2
BE058946 similar to SP|Q96556|SPD1_ Spermidine synthase 1 (EC 2.... 27 5.2
BE610004 27 6.7
TC222893 similar to UP|Q40543 (Q40543) Protein-serine/threonine ... 27 6.7
TC231576 similar to UP|Q9LSN5 (Q9LSN5) Gb|AAD22658.1 (AT3g17120/... 27 6.7
>TC223727 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (9%)
Length = 843
Score = 135 bits (341), Expect = 8e-33
Identities = 64/157 (40%), Positives = 95/157 (59%), Gaps = 1/157 (0%)
Frame = +1
Query: 5 LYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAED 64
LYKR ++C+ E ++ +VH+G G H + ++ARK+LRAGYYW TM D
Sbjct: 256 LYKRNHDMKPLRCVDAREANHMIEEVHEGSFGTHANGHAMARKILRAGYYWLTMESDCCV 435
Query: 65 YVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAWWGIDLLGPF-PKATGQLKYLVVAINYS 123
+V C KCQ D APP L + SPWPF+ WGID++G PKA+ ++++VAI+Y
Sbjct: 436 HVRKCHKCQAFADNVNAPPHPLNVMSSPWPFSMWGIDVIGAIEPKASNGHRFILVAIDYF 615
Query: 124 TKWIEVEPLAKIKAKNVLRFFKRNILARFGVPALVIS 160
TKW+E + V+RF K+ I+ R+G+P +I+
Sbjct: 616 TKWVEAASYTDVMRGVVVRFIKKEIICRYGLPRKIIT 726
>BI316922
Length = 405
Score = 117 bits (294), Expect = 2e-27
Identities = 59/132 (44%), Positives = 84/132 (62%)
Frame = +3
Query: 29 DVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAEDYVNLCDKCQRHGDMHLAPPVELTS 88
++H GICG H + + +VLR GYY TM K +YV C++C + G++ EL +
Sbjct: 3 EMHRGICGMHSKSQLMTTRVLRVGYY**TMRKYCTEYVKKCEEC*KFGNISHLLVEELHN 182
Query: 89 LVSPWPFAWWGIDLLGPFPKATGQLKYLVVAINYSTKWIEVEPLAKIKAKNVLRFFKRNI 148
+V+PWPFA G+D+L PFP + Q+KYL+V I+ TKWIE E +A I NV +F RNI
Sbjct: 183 IVAPWPFAI*GVDILRPFPLSKRQVKYLLVGIDQFTKWIETEHIAIISIANVRKFV*RNI 362
Query: 149 LARFGVPALVIS 160
+ FG+P +IS
Sbjct: 363 VC*FGIPNTLIS 398
>BI321666
Length = 430
Score = 84.7 bits (208), Expect = 2e-17
Identities = 40/116 (34%), Positives = 70/116 (59%)
Frame = +2
Query: 44 LARKVLRAGYYWPTMLKDAEDYVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAWWGIDLL 103
++ VL++ +Y P++ KDA + C+KCQR + + L +++ F +WGID +
Sbjct: 2 ISTNVLQSRFYLPSIFKDAYVHAQSCNKCQRTRSVSKRNELPLHTILEVEIFDYWGIDFV 181
Query: 104 GPFPKATGQLKYLVVAINYSTKWIEVEPLAKIKAKNVLRFFKRNILARFGVPALVI 159
GPFP + +Y++V ++Y +KW+E K AK V++F K+ I +R GVP ++I
Sbjct: 182 GPFPPSFSN-EYILVVVDYVSKWVEAVACQKSDAKIVIKFLKKQIFSRLGVPWVLI 346
>AW184779
Length = 432
Score = 67.4 bits (163), Expect(2) = 3e-14
Identities = 29/69 (42%), Positives = 44/69 (63%)
Frame = +1
Query: 5 LYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAED 64
LYKR L++C+ E +++L +VH+G G H ++ ++A+K+LR GYYW TM D
Sbjct: 16 LYKRNHDMVLLRCVDAREAEQMLVEVHEGSFGTHANIHAMAQKILRVGYYWLTMESDCCI 195
Query: 65 YVNLCDKCQ 73
+V C KCQ
Sbjct: 196HVWKCHKCQ 222
Score = 42.0 bits (97), Expect = 2e-04
Identities = 27/86 (31%), Positives = 45/86 (51%), Gaps = 18/86 (20%)
Frame = +3
Query: 75 HGDMHLAPPVELT--------SLVSPWPF---AW------WGIDLLGPF-PKATGQLKYL 116
+G+ L P VE+ +++P P+ +W WGID++G PKA+ ++
Sbjct: 174 YGERLLHPCVEMP*VPVRPSLIMLTPHPYL*MSWQHLGHMWGIDVIGAIEPKASNGHHFI 353
Query: 117 VVAINYSTKWIEVEPLAKIKAKNVLR 142
+VAI+Y TKW+E A + V+R
Sbjct: 354 LVAIDYFTKWVEAVSYASVTRSVVIR 431
Score = 26.9 bits (58), Expect(2) = 3e-14
Identities = 11/29 (37%), Positives = 14/29 (47%)
Frame = +2
Query: 67 NLCDKCQRHGDMHLAPPVELTSLVSPWPF 95
N Q D APP+ L L +PWP+
Sbjct: 206 NAISASQTFADNVNAPPIPLNVLAAPWPY 292
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 55.1 bits (131), Expect = 2e-08
Identities = 45/152 (29%), Positives = 71/152 (46%), Gaps = 10/152 (6%)
Frame = +1
Query: 25 EVLADVHDGICGQHLDV-KSLARKVLRAGYYWPTMLKDAEDYVNLCDKCQRHGDMHLAPP 83
++L + H G H + ++LAR L+A +YWP M +D + Y+ C CQ+ + P
Sbjct: 3217 KILQEYHSSPIGGHAGITRTLAR--LKAQFYWPKMQEDVKAYIQKCLICQQAKSNNTLPA 3390
Query: 84 VELTSLVSPWPFAWW---GIDLLGPFPKATGQLKYLVVAINYSTKWIEVEPL-AKIKAKN 139
L L P P W +D + P + G L ++V I+ TK+ PL A +K
Sbjct: 3391 GLLQPL--PIPQQVWEDVAMDFITGLPNSFG-LSVIMVVIDRLTKYAHFIPLKADYNSKV 3561
Query: 140 VLRFFKRNILARFGVPALVIS-----VLSRFW 166
V F +I+ G+P ++S S FW
Sbjct: 3562 VAEAFMSHIVKLHGIPRSIVSDRDRVFTSTFW 3657
>TC212032 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (3%)
Length = 803
Score = 54.3 bits (129), Expect = 3e-08
Identities = 23/51 (45%), Positives = 33/51 (64%)
Frame = +2
Query: 5 LYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYW 55
LYKR L+ C+ E + +L +VH+G G H + ++ARK+LRAGYYW
Sbjct: 650 LYKRNHDMVLLHCVNGKEVENMLGEVHEGSFGTHSNGHAMARKILRAGYYW 802
>CO982196
Length = 812
Score = 50.1 bits (118), Expect = 6e-07
Identities = 42/145 (28%), Positives = 65/145 (43%), Gaps = 4/145 (2%)
Frame = +1
Query: 26 VLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAEDYVNLCDKCQRHGDMHLAPPVE 85
+L ++ D G H ++V +W M K DYV C+ C+R+ L+P
Sbjct: 376 LLKELQDSPLGGHSGFFRTFKRVANV-VFWQGMKKTTRDYVAACEICRRNKTSTLSPAGL 552
Query: 86 LTSLVSPWPFAWW---GIDLLGPFPKATGQLKYLVVAINYSTKWIEVEPLA-KIKAKNVL 141
L L P P W +D +G PKA G+ LVV ++ TK+ L+ AK V
Sbjct: 553 L*LL--PIPTKVWTDISMDFIGGLPKAQGKDNILVV-VDRLTKYAHFFALSHPYTAKEVA 723
Query: 142 RFFKRNILARFGVPALVISVLSRFW 166
F + ++ G PA ++S R +
Sbjct: 724 ELFIKELVRLHGFPASIVSDXXRLF 798
>TC206741
Length = 1191
Score = 39.7 bits (91), Expect = 8e-04
Identities = 14/27 (51%), Positives = 19/27 (69%)
Frame = -1
Query: 50 RAGYYWPTMLKDAEDYVNLCDKCQRHG 76
++ +YWPT+ K A +YV CD CQR G
Sbjct: 303 QSSFYWPTLWKGAYEYVQTCDNCQRTG 223
>BE801213 weakly similar to GP|6691193|gb| F7F22.17 {Arabidopsis thaliana},
partial (3%)
Length = 416
Score = 32.7 bits (73), Expect = 0.094
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +3
Query: 137 AKNVLRFFKRNILARFGVPALVIS 160
AK V++F K+NI +RFG+P ++IS
Sbjct: 9 AKIVIKFLKKNIFSRFGMPRILIS 80
>TC219268
Length = 1407
Score = 32.0 bits (71), Expect = 0.16
Identities = 15/35 (42%), Positives = 20/35 (56%)
Frame = -3
Query: 63 EDYVNLCDKCQRHGDMHLAPPVELTSLVSPWPFAW 97
+D NL + HG + LA P +LV PWP+AW
Sbjct: 181 DDPENLLNDGTSHGLVALAVPSPSHALVRPWPWAW 77
>AW507721 weakly similar to GP|27764548|gb polyprotein {Glycine max}, partial
(3%)
Length = 464
Score = 31.2 bits (69), Expect = 0.27
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 4/71 (5%)
Frame = +1
Query: 20 PNETQ---EVLADVHDGICGQHLDV-KSLARKVLRAGYYWPTMLKDAEDYVNLCDKCQRH 75
PN++Q ++ + H G H +++ R + A +YWP M +D +V C CQ+
Sbjct: 253 PNDSQLLKMIMTESHASKVGGHAGTTRTIVR--INAQFYWPKMREDIMKFVQECVICQQA 426
Query: 76 GDMHLAPPVEL 86
H P L
Sbjct: 427 KVTHSLLPAGL 459
>TC219333 homologue to UP|Q42437 (Q42437) HD-ZIP protein, partial (28%)
Length = 751
Score = 28.1 bits (61), Expect = 2.3
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 2/49 (4%)
Frame = +3
Query: 82 PPVELTSLVSPWPFAWWGIDLLGPFPKATG--QLKYLVVAINYSTKWIE 128
PP+ L ++ PWP + +LGP P A G QL + + ST +E
Sbjct: 237 PPLVLPRVIIPWPMP---MPMLGPCPMARGLPQLPFHTGRLMLSTNDLE 374
>TC203529 UP|Q6Y1F1 (Q6Y1F1) Alpha-tubulin (Fragment), partial (26%)
Length = 303
Score = 27.7 bits (60), Expect = 3.0
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = +1
Query: 93 WPFAWWGID---LLGPFPKATGQLKY 115
WP +W D +LGP+ KA GQL +
Sbjct: 217 WPLYYWERDS*SVLGPYQKACGQLHW 294
>BM308155 weakly similar to GP|14279688|gb receptor-like kinase Xa21-binding
protein 3 {Oryza sativa}, partial (28%)
Length = 433
Score = 27.3 bits (59), Expect = 4.0
Identities = 12/38 (31%), Positives = 18/38 (46%)
Frame = -3
Query: 3 GTLYKRGFITPLIKCLGPNETQEVLADVHDGICGQHLD 40
G+ K F+ L +CLGP+ Q + H +C D
Sbjct: 128 GS*EKGVFVAKLQQCLGPHAAQHGIGHAHGSLCNSAPD 15
>TC224002 weakly similar to UP|Q9LG26 (Q9LG26) F14J16.11, partial (8%)
Length = 602
Score = 27.3 bits (59), Expect = 4.0
Identities = 15/51 (29%), Positives = 21/51 (40%)
Frame = +1
Query: 13 PLIKCLGPNETQEVLADVHDGICGQHLDVKSLARKVLRAGYYWPTMLKDAE 63
PL K L T D ++ ICG L A + + G W T L + +
Sbjct: 34 PLSKLLEGGNTCAANCDTYNEICGSPLSEGLFADQAKKVGIKWKTKLTECK 186
>BI786481 similar to GP|10177293|db ATP-dependent RNA helicase-like protein
{Arabidopsis thaliana}, partial (24%)
Length = 421
Score = 26.9 bits (58), Expect = 5.2
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +3
Query: 52 GYYWPTMLKDAEDYVNLCDKCQRHGDM 78
G YW T+ + Y + C C RHG++
Sbjct: 336 GNYWVTIPEGKSIYKSNC*SCNRHGEI 416
>BE058946 similar to SP|Q96556|SPD1_ Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1).,
partial (13%)
Length = 432
Score = 26.9 bits (58), Expect = 5.2
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 88 SLVSPWPFAWWGIDLLGPFPKATGQLKYLVVAINYSTK 125
S+ + WP+++W I L PF K + K V Y++K
Sbjct: 26 SISADWPYSYWHIQL--PF*KQSPMAK*TSVVYQYTSK 133
>BE610004
Length = 373
Score = 26.6 bits (57), Expect = 6.7
Identities = 14/54 (25%), Positives = 22/54 (39%), Gaps = 4/54 (7%)
Frame = -3
Query: 54 YWPTMLKDAEDYVNLCDKCQRHGDMHLAPPVELTSLVSPW----PFAWWGIDLL 103
YW L D ++ LC +C MH L+ + W P+ W G ++
Sbjct: 365 YWDFAL*DIFQHLLLCHQCLSQKQMHQFSHRSLSISLDQWFHNEPWDWQGFQIV 204
>TC222893 similar to UP|Q40543 (Q40543) Protein-serine/threonine kinase ,
partial (46%)
Length = 779
Score = 26.6 bits (57), Expect = 6.7
Identities = 15/47 (31%), Positives = 21/47 (43%), Gaps = 8/47 (17%)
Frame = +3
Query: 87 TSLVSPWP--------FAWWGIDLLGPFPKATGQLKYLVVAINYSTK 125
+S+ S W F+WW F A+G LKYL I +T+
Sbjct: 369 SSIASSWSENLKRNSHFSWWNHQNKDRFASASGILKYLYKEIQKATQ 509
>TC231576 similar to UP|Q9LSN5 (Q9LSN5) Gb|AAD22658.1 (AT3g17120/K14A17_24),
partial (20%)
Length = 647
Score = 26.6 bits (57), Expect = 6.7
Identities = 14/27 (51%), Positives = 19/27 (69%)
Frame = -3
Query: 144 FKRNILARFGVPALVISVLSRFWGIKS 170
F R+ILARF + + VIS++S FW S
Sbjct: 90 FIRSILARFNMASSVISLIS-FWSAAS 13
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.143 0.463
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,966,552
Number of Sequences: 63676
Number of extensions: 127298
Number of successful extensions: 745
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 742
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 742
length of query: 179
length of database: 12,639,632
effective HSP length: 91
effective length of query: 88
effective length of database: 6,845,116
effective search space: 602370208
effective search space used: 602370208
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 56 (26.2 bits)
Medicago: description of AC135504.12